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1.
Prog Orthod ; 24(1): 4, 2023 Feb 06.
Article in English | MEDLINE | ID: mdl-36740663

ABSTRACT

BACKGROUND: Smile attractiveness is a primary factor for patients to seek orthodontic treatment, however, there is yet no systematic evaluation of this topic in the literature. OBJECTIVES: To assess the current evidence on the effect of orthodontic treatment on smile attractiveness. SEARCH METHODS: Seven electronic databases (MEDLINE, Cochrane Library, Virtual Health Library, SCOPUS, Web of Science, Google Scholar and Embase) were searched on 14 September 2022. SELECTION CRITERIA: Studies evaluating smile attractiveness before and after orthodontic treatment or only after completion of orthodontic treatment. DATA COLLECTION AND ANALYSIS: Extracted data included study design and setting, sample size and demographics, malocclusion type, treatment modality and method for outcome assessment. Risk of bias was assessed with the ROBINS-I tool for non-randomised studies. Random-effects meta-analyses of mean differences and their 95% confidence intervals (CIs) were planned a priori. METHODS: After elimination of duplicate studies, data extraction and risk of bias assessment according to the Cochrane guidelines, an evaluation of the overall evidence was performed. The included studies were evaluated based on the characteristics of their study and control groups and based on their main research question. Also, all outcome measures were standardized into a common assessment scale (0-100), in order to obtain more easily interpretable results. RESULTS: Ten studies were included in this review, nine of which were assessed as being at serious risk of bias and one at moderate risk of bias. The large heterogeneity between the included studies did not allow for a meta-analysis. Orthodontic treatment has a moderately positive effect on smile attractiveness. When compared to no treatment, orthodontic treatment with premolar extractions improves smile attractiveness by 22%. Also, surgical correction of Class III cases increases smile attractiveness by 7.5% more than camouflage treatment. No other significant differences were shown between different types of treatment. CONCLUSION: Based on the available data, orthodontic treatment seems to moderately improve the attractiveness of the smile. There is significant bias in the current literature assessing the effect of orthodontics on smile attractiveness; therefore, the results cannot be accepted with certainty.


Subject(s)
Malocclusion , Orthodontics , Humans , Malocclusion/therapy , Extraoral Traction Appliances
2.
J Dent Res ; 101(1): 30-36, 2022 01.
Article in English | MEDLINE | ID: mdl-34237225

ABSTRACT

Differences in effect estimates between early primary trials included in a meta-analysis and the pooled estimate of meta-analysis might indicate potential novelty bias. The objective of this study was to assess the presence of novelty bias in a sample of studies published in periodontology and implant dentistry. On August 7, 2020, we searched the PubMed database for meta-analyses of clinical studies published between August 2015 and August 2020. Meta-analyses with at least 4 primary studies were selected for assessment. We fitted logistic regression models using trial characteristics as predictors to assess the association between these characteristics and 1) the odds of the first trial's estimate to be included in the meta-analysis confidence interval (CI) and 2) the odds of overlap between the first trial's CI and the meta-analysis prediction interval (PI). Ninety-two meta-analyses provided data for assessment. In absolute values, 70% of the meta-analyses have a pooled estimate smaller than the corresponding estimate of the first trial, although there was overlap of the CI of estimates from the first trial and the meta-analysis in 87% of the cases. This is probably due to the small number of trials in most meta-analyses and the subsequently large uncertainty associated with the pooled effect estimate. As the number of trials in the meta-analysis increased, the odds of the treatment effect estimate of the first trial to be included in the meta-analysis CI decreased by 15% for every additional trial (odds ratio, 0.85; 95% CI, 0.73 to 0.96). Meta-analytic effect estimates appear to be more conservative than those from the first trial in the meta-analysis. Our findings show evidence of novelty bias in periodontology and implant dentistry; therefore, clinicians should be aware of the risk of making decisions based on the information reported in new trials because of the risk of exaggerated estimates in these trials.


Subject(s)
Dental Implants , Periodontics , Bias , Clinical Trials as Topic , Humans , Meta-Analysis as Topic
3.
J Dent Res ; 100(3): 245-252, 2021 03.
Article in English | MEDLINE | ID: mdl-33054504

ABSTRACT

Research waste is highly prevalent across biomedical investigations. We aimed to assess the evidence on the extent of research waste in dental research. We performed a scoping review of empirical evaluations of dental studies assessing the prevalence and impact of limitations in design, conduct, analysis, and reporting of research. PubMed was searched using specific terms to retrieve studies dealing with design, conduct, analysis, and reporting of studies in dentistry, with no year or language restrictions. Of the 1,807 publications identified from the search and from manual searches, 71 were included in this review. The topic and article selection was based on the expert opinion of the authors. The existing evidence suggests that, although there are improvements over time, substantial deficiencies in all areas (design, conduct, analysis, reporting) were prevalent in dental research publications. Waste in research is a multifaceted problem without a simple solution. However, an appreciation of optimal research design and execution is a prerequisite and should be underpinned by policies that include appropriate training in research methods and properly aligned incentives.


Subject(s)
Dental Research , Research Design
4.
Int J Oral Maxillofac Surg ; 49(3): 403-410, 2020 Mar.
Article in English | MEDLINE | ID: mdl-31466829

ABSTRACT

An assessment of the quality of oral and maxillofacial surgery clinical practice guidelines is lacking. The aim of this investigation was to assess the quality of guidelines using the RIGHT (Reporting Items for practice Guidelines in HealThcare) checklist. The primary outcome was to assess the score (quality) of guidelines based on the RIGHT checklist and to identify predictors (region, type, single or multi-centre, and speciality/non-speciality) influencing the quality score. In this review, following a search of electronic databases and national society websites, a total of 25 guidelines were independently assessed by two assessors against the 22-item RIGHT checklist. Inter-assessor reliability was assessed. Deficiencies in the reporting of items relating to limitations, funding, declaration and management of interests, healthcare questions, and quality assurance were evident. The median overall score for the guidelines was 28 (range 14-66). Guidelines produced by multiple centres (ß=57.15, 95% confidence interval -26.62 to 87.68, P= 0.001, multivariate analysis) and non-speciality societies (ß=20, 95% confidence interval -0.03 to 40.03, P=0.05, univariate analysis) tended to have higher quality scores. Overall, the quality of clinical practice guidelines used in oral and maxillofacial surgery was deemed suboptimal. If clinical practice guidelines are to be used in making treatment decisions for patients, clinicians should be aware of their possible limitations.


Subject(s)
Checklist , Surgery, Oral , Humans , Practice Guidelines as Topic , Reproducibility of Results , Research Design
6.
J Orofac Orthop ; 81(1): 22-29, 2020 Jan.
Article in English | MEDLINE | ID: mdl-31720733

ABSTRACT

PURPOSE: To evaluate the residual surface roughness of 5 common diamond-coated interproximal reduction (IPR) systems after consecutive in vitro applications in relation to system, diamond grain size, and instrument thickness. METHODS: IPR was performed on 80 extracted human incisors using motor-driven strips and discs under predefined conditions. The IPR auxiliaries were applied at 5 consecutive sessions of 20 s on intact interproximal surfaces, and the surface profile (Ra, Rz, Rmax) was analyzed at baseline and after each session with an optical profilometer. RESULTS: No overall significant difference in the roughness values was found between systems (P = 0.07 for Ra, P = 0.33 for Rz, and P = 0.48 for Rmax). There was a significant average decrease of Ra, Rz, and Rmax for all systems for every unit increase in time by -0.171 µm (P < 0.001), -3.297 (P ≤ 0.001), and -2.788 µm (P = 0.001), respectively. Ra, Rz, and Rmax values increased significantly, i.e., by 0.194 µm (P = 0.003), 5.890 µm (P = 0.001), and 5.319 µm (P = 0.010) as instrument thickness increased by one unit. No significant reductions in Ra, Rz, and Rmax were observed across grain sizes (-0.008 µm [P > 0.05], -0.244 µm [P > 0.05], and -0.179 µm [P > 0.05], respectively). There was no evidence of interaction between system and time as the P values for Ra, Rz, and Rmax were 0.88, 0.51, and 0.70, respectively. CONCLUSIONS: All IPR materials presented significant gradual decrease of surface roughness after repeated applications. There were no significant roughness changes among auxiliaries of different grain sizes. Thinner auxiliaries showed significantly more roughness reduction, possibly requiring more frequent replacement than thick auxiliaries in clinical practice.


Subject(s)
Diamond , Incisor , Humans , Materials Testing , Surface Properties
7.
Eur Arch Paediatr Dent ; 21(4): 387-406, 2020 Aug.
Article in English | MEDLINE | ID: mdl-31602555

ABSTRACT

PURPOSE: To systematically review the evidence of the diagnostic efficacy of panoramic radiographs (PRs) in the following six clinical situations: caries, acute dental infections, dental trauma, dental anomalies, Developmental disorders and pathological conditions in children. The research question posed by the EAPD was: "In which clinical situations and age groups is it indicated/contra-indicated to prescribe PRs in relation to these six clinical situations, in children". METHODS: An electronic search of the following databases was conducted: MEDLINE via OVID, EMBASE via OVID, The Cochrane Oral Health Group's Trials Register, the Cochrane Central Register of Controlled Trials (CENTRAL), The Cochrane Database of Systematic Reviews (CDSR), the Database of Abstracts of Reviews of Effects (DARE) and the Cochrane Health Technology Assessment (HTA), to identify relevant studies published from 1990 to 2018. The systematic review was performed according to the PRISMA guidelines. Study screening, data extraction and risk of bias assessment of included studies was carried out independently and in duplicate using the QUADAS 2 tool. RESULTS: The search resulted in 3420 potentially relevant studies, and following screening 175 full texts were obtained, of which 16 were finally included in the review. Meta-analysis was not possible for any of the aforementioned clinical situations, therefore the included studies were tabulated in terms of qualitative descriptive hierarchy of evidence for diagnostic. CONCLUSION: Based on currently available evidence there is a need for high quality diagnostic accuracy efficacy studies to address important diagnostic tasks in these six clinical situations in children, as there is a lack of scientific evidence concerning PRs in children, as there would support the production of guidelines for prescribing PRs in children along with their relative indications and contra-indications.


Subject(s)
Dental Caries , Radiography, Panoramic , Child , Humans , Oral Health
8.
Br J Oral Maxillofac Surg ; 57(5): 412-418, 2019 06.
Article in English | MEDLINE | ID: mdl-31054792

ABSTRACT

The aim and objective of this study was to evaluate the quality and readability of leaflet and online Oral and Maxillofacial Surgery patient information leaflets (PILs). The quality, readability and grade level of each PIL was assessed using the DISCERN, Flesch Reading Ease and Flesh-Kincaid Grade Level instruments respectively. In total, 140 patient information leaflets were assessed. For both leaflet and online PILs, many items of the DISCERN instrument were deemed of low quality and poorly reported. The median overall quality score was 30.2. Variation in the quality and readability scores between leaflet and online PILs and those produced by various societies was evident. Overall, PILs were deemed to be of moderate quality. Online PILs were of lower quality, more difficult to read and aimed at a higher reading age level.


Subject(s)
Comprehension , Pamphlets , Patient Education as Topic/methods , Reading , Surgery, Oral , Humans , Internet , Patient Education as Topic/standards , Publications/standards , Records
9.
Br J Dermatol ; 180(3): 534-552, 2019 03.
Article in English | MEDLINE | ID: mdl-30609010

ABSTRACT

Evidence shows that the quality of reporting of randomised controlled trials (RCTs) is not optimal. The lack of transparent reporting impedes readers from judging the reliability and validity of trial findings and researchers from extracting information for systematic reviews and results in research waste. The Consolidated Standards of Reporting Trials (CONSORT) statement was developed to improve the reporting of RCTs. Within person trials are used for conditions that can affect two or more body sites, and are a useful and efficient tool because the comparisons between interventions are within people. Such trials are most commonly conducted in ophthalmology, dentistry, and dermatology. The reporting of within person trials has, however, been variable and incomplete, hindering their use in clinical decision making and by future researchers. This document presents the CONSORT extension to within person trials. It aims to facilitate the reporting of these trials. It extends 16 items of the CONSORT 2010 checklist and introduces a modified flowchart and baseline table to enhance transparency. Examples of good reporting and evidence based rationale for CONSORT within person checklist items are provided.


Subject(s)
Checklist/standards , Publishing/standards , Randomized Controlled Trials as Topic/standards , Research Design/standards , Humans , Reproducibility of Results
10.
Prog Orthod ; 19(1): 16, 2018 Jun 11.
Article in English | MEDLINE | ID: mdl-29888375

ABSTRACT

BACKGROUND: To assess patterns of research collaboration in orthodontics and possible relationships with sample size and funding status. METHODS: Orthodontic randomised and non-randomised controlled clinical trials published between 2013 and 2017 were identified through electronic searching. The nature of collaboration, author institutions, study setting, sample size, and funding status were assessed. Linear and logistic regression analyses were applied. RESULTS: Of 1153 studies, 217 met the selection criteria. The majority of studies were authored by university academics (86%), were conducted in a single centre (71.9%) and in at least one university hospital (68.2%). The number of practice-based trials (10.1%), as well as the involvement of specialist practitioners (5.2%) in co-authorship, was limited. Multi-centred studies within a single country were associated with a significantly larger sample size compared to single-centred trials (P = 0.00; 95% confidence interval [CI] 33.59, 106.93). However, authorship collaboration either nationally (odds ratio [OR] 2.37; 95% CI 0.85, 6.57) or internationally across different continents (OR 5.54; 95% CI 0.62, 49.52) did not translate into increased funding. CONCLUSIONS: Most orthodontic studies were undertaken in university hospital settings within a single country. Collaboration is common in orthodontics but involvement of practice settings remains limited, suggesting a need for stimulation of practice-based research and research partnerships.


Subject(s)
Controlled Clinical Trials as Topic , Cooperative Behavior , Dental Research , Orthodontics , Research Support as Topic , Humans , Research Design , Sample Size
11.
Orthod Craniofac Res ; 2018 Apr 06.
Article in English | MEDLINE | ID: mdl-29624856

ABSTRACT

OBJECTIVES: To compare the relationship between Riedel and Wits appraisal in skeletal III malocclusions and to assess the effect of variation in the vertical skeletal measurements. SETTING AND SAMPLE POPULATION: One-hundred and twenty consecutive subjects with Class III malocclusions attending orthognathic clinics were analysed. MATERIALS AND METHODS: Lateral cephalograms were hand-traced under standardized conditions. Cephalometric measurements included Riedel analysis, Wits appraisal, A point/B point to Frankfurt plane (AF-BF) and vertical measurements. RESULTS: The mean ANB and Wits values were -3.22° and -11.39 mm, respectively. A positive correlation was found between ANB and Wits overall (r = -.49; P < .001). However, there was poor agreement between both Wits (r = .087) and Riedel (r = .089) with AF-BF (P > .05). A negative correlation existed between Wits and LAFH% (r = -.64, P-value < .001); however, no relationship was observed between ANB and LAFH% (P > .05) based on linear regression analysis. CONCLUSIONS: There is a positive relationship between ANB and Wits; however, neither correlated well with a purer antero-posterior assessment (AF-BF). Furthermore, variations in the vertical skeletal measurements affect the strength of agreement. Caution is advised in the interpretation of antero-posterior analysis especially in the presence of vertical discrepancy.

12.
Orthod Craniofac Res ; 21(1): 27-32, 2018 Feb.
Article in English | MEDLINE | ID: mdl-29205804

ABSTRACT

OBJECTIVES: To investigate the appearance of three esthetic nickel titanium (NiTi) wires after 6 weeks of intra-oral cycling and to determine the association between objective and subjective measures of esthetics. SETTING AND SAMPLE POPULATION: A prospective cohort study was undertaken involving participants undergoing upper fixed orthodontic appliance treatment with ceramic brackets. MATERIALS AND METHODS: Fifty participants were assigned to one of three groups of NiTi esthetic wires (American Orthodontics Ever White™, Forestadent Biocosmetic™ and GAC High Aesthetic™), with wires retrieved after 6 weeks in situ. Participants completed a bespoke questionnaire exploring perceptions of wire esthetics. Objective measurement of coating loss was undertaken using a custom arch wire jig. RESULTS: American Orthodontics Ever White™ had the greatest mean coating loss (50.7%) followed by Forestadent Biocosmetic™ (6%), with GAC High Aesthetic TM undergoing minimal loss (0.07%) (P < .001). The majority of coating loss with the American Orthodontics Ever White™ wires arose in the anterior region while Forestadent Biocosmetic™ wires and GAC High Aesthetic™ wires exhibited coating loss posteriorly (P < .001). These findings were reflected in the subjective assessment with a negative correlation found between coating loss and final Visual Analogue Scale scores (P < .001). CONCLUSIONS: Considerable esthetic variation between arch wires following 6 weeks of intraoral cycling was identified in this prospective cohort study. Intraoral cycling has a negative impact on participant perception of arch wire esthetics, and objective and subjective assessment of wire esthetics appears to be consistent.


Subject(s)
Esthetics, Dental , Orthodontic Appliance Design , Orthodontic Wires , Adult , Device Removal , Female , Humans , London , Male , Materials Testing , Nickel , Orthodontic Brackets , Prospective Studies , Surface Properties , Surveys and Questionnaires , Titanium
13.
J Periodontal Res ; 53(3): 267-287, 2018 Jun.
Article in English | MEDLINE | ID: mdl-29211309

ABSTRACT

The cholesterol-lowering drugs, statins, possess anti-inflammatory, antimicrobial and pro-osteogenic properties, and thus have been tested as an adjunct to periodontal treatment. The present systematic review aimed to answer the following focused research question: What is the effect of local and/or systemic statin use on periodontal tissues in preclinical in vivo studies of experimentally induced periodontitis (EIP) and/or acute/chronified periodontal defect (ACP) models? A literature search (of Medline/PubMed, Embase/Ovid, CENTRAL/Ovid) using the following main eligibility criteria was performed: (i) English or German language; (ii) controlled preclinical in vivo trials; (iii) local and/or systemic statin use in EIP and/or ACP models; and (iv) quantitative evaluation of periodontal tissues (i.e., alveolar bone level/amount, attachment level, cementum formation, periodontal ligament formation). Sixteen studies in EIP models and 7 studies in ACP models evaluated simvastatin, atorvastatin or rosuvastatin. Thirteen of the EIP (81%) and 2 of the ACP (29%) studies presented significantly better results in terms of alveolar bone level/amount in favor of statins. Meta-analysis based on 14 EIP trials confirmed a significant benefit of local and systemic statin use (P < .001) in terms of alveolar bone level/amount; meta-regression revealed that statin type exhibited a significant effect (P = .014) in favor of atorvastatin. Three studies reported a significantly higher periodontal attachment level in favor of statin use (P < .001). Complete periodontal regeneration was never observed; furthermore, statins did not exert any apparent effect on cementum formation. Neither local nor systemic use of statins resulted in severe adverse effects. Statin use in periodontal indications has a positive effect on periodontal tissue parameters, supporting the positive results already observed in clinical trials. Nevertheless, not all statins available have been tested so far, and further research is needed to identify the maximum effective concentration/dose and optimal carrier.


Subject(s)
Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use , Periodontitis/drug therapy , Periodontitis/surgery , Animals , Humans , Hydroxymethylglutaryl-CoA Reductase Inhibitors/pharmacology , Randomized Controlled Trials as Topic
15.
Eur Arch Paediatr Dent ; 17(6): 445-454, 2016 Dec.
Article in English | MEDLINE | ID: mdl-27866328

ABSTRACT

AIM: This in vitro study was to classify questionable for caries occlusal surfaces (QCOS) of permanent teeth according to ICDAS codes 1, 2, and 3 and to compare them in terms of enamel mineral composition with the areas of sound tissue of the same tooth. METHODS: Partially impacted human molars (60) extracted for therapeutic reasons with QCOS were used in the study, photographed via a polarised light microscope and classified according to the ICDAS II (into codes 1, 2, or 3). The crowns were embedded in clear self-cured acrylic resin and longitudinally sectioned at the levels of the characterised lesions and studied by SEM/EDX, to assess enamel mineral composition of the QCOS. Univariate and multivariate random effect regressions were used for Ca (wt%), P (wt%), and Ca/P (wt%). RESULTS: The EDX analysis indicated changes in the Ca and P contents that were more prominent in ICDAS-II code 3 lesions compared to codes 1 and 2 lesions. In these lesions, Ca (wt%) and P (wt%) concentrations were significantly decreased (p = 0.01) in comparison with sound areas. Ca and P (wt%) contents were significantly lower (p = 0.02 and p = 0.01 respectively) for code 3 areas in comparison with codes 1 and 2 areas. Significantly higher (p = 0.01) Ca (wt%) and P (wt%) contents were found on sound areas compared to the lesion areas. CONCLUSIONS: The enamel of occlusal surfaces of permanent teeth with ICDAS 1, 2, and 3 lesions was found to have different Ca/P compositions, necessitating further investigation on whether these altered surfaces might behave differently on etching preparation before fissure sealant placement, compared to sound surfaces.


Subject(s)
Dental Caries/pathology , Dental Enamel/chemistry , Dental Enamel/diagnostic imaging , Dental Enamel/pathology , Dentition, Permanent , Calcium/analysis , Composite Resins/chemistry , Dental Caries/diagnosis , Dental Caries/diagnostic imaging , Humans , Molar, Third/diagnostic imaging , Molar, Third/pathology , Phosphorus/analysis , Radiography, Dental, Digital , Surface Properties
16.
J Oral Rehabil ; 42(12): 914-25, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26132229

ABSTRACT

The purpose of this study was to examine the reporting quality of randomised controlled trials (RCTs) published in prosthodontic and implantology journals. Thirty issues of nine journals in prosthodontics and implant dentistry were searched for RCTs, covering the years 2005-2012: The Journal of Prosthetic Dentistry, Journal of Oral Rehabilitation, The International Journal of Prosthodontics, The International Journal of Periodontics & Restorative Dentistry, Clinical Oral Implants Research, Clinical Implant Dentistry & Related Research, The International Journal of Oral & Maxillofacial Implants, Implant Dentistry and Journal of Dentistry. The reporting quality was assessed using a modified Consolidated Standards of Reporting Trials (CONSORT) statement checklist. Data were analysed using descriptive statistics followed by univariable and multivariable examination of statistical associations (α = 0·05). A total of 147 RCTs were identified with a mean CONSORT score of 69·4 (s.d. = 9·7). Significant differences were found among journals with the Journal of Oral Rehabilitation achieving the highest score (80·6, s.d. = 5·5) followed by Clinical Oral Implants Research (73·7, s.d. = 8·3). Involvement of a statistician/methodologist was significantly associated with increased CONSORT scores. Overall, the reporting quality of RCTs in major prosthodontic and implantology journals requires improvement. This is of paramount importance considering that optimal reporting of RCTs is an important prerequisite for clinical decision-making.


Subject(s)
Dental Implantation , Dental Research , Periodicals as Topic , Prosthodontics , Randomized Controlled Trials as Topic/standards , Bibliometrics , Checklist , Humans
17.
J Dent ; 42(10): 1277-83, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25123352

ABSTRACT

OBJECTIVES: To evaluate the effect of biannual fluoride varnish applications in preschool children as an adjunct to school-based oral health promotion and supervised tooth brushing with 1000ppm fluoride toothpaste. METHODS: 424 preschool children, 2-5 year of age, from 10 different pre schools in Athens were invited to this double-blind randomized controlled trial and 328 children completed the 2-year programme. All children received oral health education with hygiene instructions twice yearly and attended supervised tooth brushing once daily. The test group was treated with fluoride varnish (0.9% diflurosilane) biannually while the control group had placebo applications. The primary endpoints were caries prevalence and increment; secondary outcomes were gingival health, mutans streptococci growth and salivary buffer capacity. RESULTS: The groups were balanced at baseline and no significant differences in caries prevalence or increment were displayed between the groups after 1 and 2 years, respectively. There was a reduced number of new pre-cavitated enamel lesions during the second year of the study (p=0.05) but the decrease was not statistically significant. The secondary endpoints were unaffected by the varnish treatments. CONCLUSIONS: Under the present conditions, biannual fluoride varnish applications in preschool children did not show significant caries-preventive benefits when provided as an adjunct to school-based supervised tooth brushing with 1000ppm fluoride toothpaste. CLINICAL SIGNIFICANCE: In community based, caries prevention programmes, for high caries risk preschool children, a fluoride varnish may add little to caries prevention, when 1000ppm fluoride toothpaste is used daily.


Subject(s)
Cariostatic Agents/therapeutic use , Dental Caries/prevention & control , Fluorides, Topical/therapeutic use , Health Promotion , Oral Health , Toothbrushing/methods , Buffers , Child, Preschool , DMF Index , Dental Caries/microbiology , Dental Caries Susceptibility/physiology , Dental Plaque Index , Double-Blind Method , Drug Combinations , Fluorides/therapeutic use , Follow-Up Studies , Health Education, Dental , Humans , Oral Hygiene/education , Periodontal Index , Placebos , Polyurethanes/therapeutic use , Saliva/microbiology , Saliva/physiology , School Dentistry/methods , Silanes/therapeutic use , Streptococcus mutans/isolation & purification , Toothpastes/therapeutic use , Treatment Outcome
18.
Tumour Biol ; 35(10): 9597-602, 2014 Oct.
Article in English | MEDLINE | ID: mdl-24961351

ABSTRACT

The Ets-related gene fusions are among the most common molecular alterations in prostate cancer (PCa) and are detected in more than 50 % of PCas. Transmembrane protease serine 2 and Ets-related gene fusion (TMPRSS2-ERG) is the most frequently identified chimeric gene and has been associated with undifferentiated and invasive phenotypes. TMPRSS2-ERG has also been detected in prostate intraepithelial neoplasia (PIN) lesions and more rarely in benign prostatic hyperplasia (BPH) regions mainly in PCa-bearing glands. The possibility that the fusion TMPRSS2-ERG may be present in BPH samples in the absence of apparent PCa was addressed. Out of 115 BPH samples, three were found positive employing RT-PCR. The presence of the fusion gene was confirmed by FISH for these samples, and an additional four samples were found to carry the TMPRSS2-ERG fusion out of 43 tested by the later approach. The presence of the TMPRSS2-ERG fusion did not result in altered expression of 12 putative downstream targets. These findings indicate that TMPRSS2-ERG may or may not lead to PCa development.


Subject(s)
Oncogene Proteins, Fusion/genetics , Prostatic Hyperplasia/genetics , Humans , In Situ Hybridization, Fluorescence , Male , Middle Aged , Reverse Transcriptase Polymerase Chain Reaction
19.
Eur J Orthod ; 36(5): 522-30, 2014 Oct.
Article in English | MEDLINE | ID: mdl-23197574

ABSTRACT

The purpose of this long-term follow-up study was twofold-firstly, to assess prevalence of relapse after treatment of deep bite malocclusion and secondly, to identify risk factors that predispose patients with deep bite malocclusion to relapse. Sixty-one former patients with overbite more than 50% incisor overlap before treatment were successfully recalled. Clinical data, morphometrical measurements on plaster casts before treatment, after treatment and at long-term follow-up, as well as cephalometric measurements before and after treatment were collected. The median follow-up period was 11.9 years. Patients were treated by various treatment modalities, and the majority of patients received at least a lower fixed retainer and an upper removable bite plate during retention. Relapse was defined as increase in incisor overlap from below 50% after treatment to equal or more than 50% incisor overlap at long-term follow-up. Ten per cent of the patients showed relapse to equal or larger than 50% incisor overlap, and their amount of overbite increase was low. Among all cases with deep bite at follow-up, gingival contact and palatal impingement were more prevalent in partially corrected noncompliant cases than in relapse cases. In this sample, prevalence and amount of relapse were too low to identify risk factors of relapse.


Subject(s)
Overbite/therapy , Adolescent , Adult , Cephalometry/methods , Child , Female , Follow-Up Studies , Gingiva/pathology , Humans , Incisor/pathology , Longitudinal Studies , Male , Malocclusion, Angle Class II/pathology , Malocclusion, Angle Class II/therapy , Models, Dental , Orthodontic Retainers , Overbite/pathology , Palate/pathology , Patient Compliance , Recurrence , Retrospective Studies , Risk Factors , Young Adult
20.
Orthod Craniofac Res ; 12(4): 305-11, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19840283

ABSTRACT

OBJECTIVES: To comparatively evaluate the extrusive forces and torquing moments on the posterior dentition generated during anterior intrusion with different intrusion techniques in the maxillary and mandibular dental arch. MATERIAL AND METHODS: Seven wire specimens were used for each of the following intrusive arches: Utility arch 0.016 x 0.016' Blue Elgiloy, Utility arch 0.017 x 0.025' TMA and Burstone Intrusion arch 0.017 x 0.025' TMA. The wires were inserted on bracketed dental arches constructed on maxillary Frasaco models, segmented mesially to the maxillary canines. Simulated intrusion from 0.0-3.0 mm was performed on the Orthodontic Measurement and Simulation System (OMSS). The forces and moments were recorded in all three planes of space at 0.1 mm increments and the values at 3.0 mm for all wires were used for all statistical evaluations. The data were analyzed, separately for the forces and moments, by means of two-way analysis of variance (ANOVA) with forces and moments serving as the dependent variables and intrusion technique and jaw (maxilla or mandible) as the independent variable. Post hoc multiple comparisons were performed using the Tukey test at .05 error rate. RESULTS: The 0.016 x 0.016' Blue Elgiloy utility arch exerted the highest posterior extrusive forces, 15% higher than the 0.017 x 0.025' TMA utility and 40% higher in comparison with the 0.017 x 0.025' TMA Burstone intrusion arch.The lowest posterior moment in the sagittal plane was generated by the 0.017 x 0.025' TMA Burstone intrusion arch. The 0.016 x 0.016' Blue Elgiloy utility arch exerted 15% higher posterior moments and the 0.017 x 0.025' TMA utility 25% higher. Forces and moments were consistently larger for the mandible compared to the maxilla for the same intrusion technique. CONCLUSIONS: The upper Burstone 0.017 x 0.025' TMA intrusion arch exerted the lowest forces/moments on posterior teeth. The highest forces were generated by the 0.016 x 0.016-inch Blue Elgiloy utility arch and the highest moments by the lower 0.017 x 0.025-inch TMA utility arch.


Subject(s)
Bicuspid/physiopathology , Dental Stress Analysis/methods , Incisor , Molar/physiopathology , Tooth Movement Techniques/adverse effects , Biomechanical Phenomena , Computer Simulation , Humans , Models, Biological , Models, Dental , Orthodontic Brackets/adverse effects , Orthodontic Wires/adverse effects , Tooth Movement Techniques/instrumentation , Tooth Movement Techniques/methods , Torque
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