Improving antibiotic removal and anaerobic digestion performance of discarded cefradine pellets through thermo-alkaline pretreatment.

Discarded cefradine pellets (DCP) as the hazardous wastes contain lots of bioavailable sucrose. Anaerobic digestion (AD) may be a promising technology for treating DCP, achieving dual goals of waste treatment and resource recovery. However, high concentration of cefradine will inhibit the AD process. This study applied thermo-alkaline pretreatment (TAP) to remove cefradine and improve the AD performance of DCP. Around 95% cefradine could be degraded to different intermediate degradation products (TPs) in TAP at optimal condition, and hydrolysis and hydrogenation were the main degradation pathways. Quantitative structure-activity relationship analysis indicated that the main TPs exhibited lower toxicity than cefradine, and DCP residues after TAP were almost not toxic to E. coli K12 and B. subtilis growth by antibacterial activity analysis. Therefore, TAP promoted the biomethane yield in AD of DCP residues (274.74 mL/g COD), which was 1.91 times that of control group. Besides, compared to control group, final cefradine concentrations in liquids and sludge were significantly decreased in AD system with TAP, lowering environmental risk and indicating stronger prospect for process application. Microbiological analysis revealed that acidogens (Macellibacteroides, Bacteroides), syntrophs (Syntrophobacter, Syntrophorhabdus), and acetoclastic Methanosaeta were enriched in AD system with TAP, which contributed to improving AD performance of DCP.

Behavioral decision-making of the government, farmer-specialized cooperatives, and farmers regarding the quality and safety of agricultural products.

The quality and safety of agricultural products is very important for farmers' professional cooperatives. This study incorporates the government, farmers' professional cooperatives and farmers into the evolutionary game model to explore the game relationship and evolutionary path of decision-making among the three parties related to the quality and safety of agricultural products. Through the dynamic analysis of decision-making replication, the analysis of strategy evolution stability and the verification of numerical simulation experiments, it is shown that the decision-making behavior of the government, farmers' professional cooperatives and farmers under the conditions of agricultural product quality and safety is the result of the game between three stakeholders; the government, farmers' professional cooperatives and farmers The evolution process of the decision-making behavior to the ideal state is affected by many factors, and the value ranges of different factors have different effects on the convergence speed of the three stakeholders to the ideal state; when certain conditions are met, the government, farmers' professional cooperatives and farmers' three The decision-making behavior of each stakeholder can evolve into an ideal state, and effective government supervision can promote the cooperatives to manage the green production of farmers, and then effectively encourage farmers to take the initiative in green production. In order to provide useful suggestions for the government to make safety supervision decisions, to effectively manage cooperatives, and to stimulate active green production behaviors by farmers.

Driving mechanism of farmers' green production behavior under normalization of COVID-19 prevention and control: A case study in China.

China's public health emergency COVID-19 has brought great challenges to food safety. Among them, the quality and safety of agricultural products under the normalization of the COVID-19 prevention and control has become a hot issue of general concern. This study attempts to reveal the driving factors and mechanisms of farmers' green production behavior. The empirical research by collecting 673 sample data shows that: individual characteristics of farmers, government guiding factors, an industrial organization promoting factors, and market adjustment factors have a positive driving effect on farmers' green production behavior. And farmers' green production behavior has a positive influence on the quality and safety of agricultural products. Farmers' green production behavior plays an intermediary role between the quality and safety of agricultural products and individual characteristics of farmers, government guidance factors, industrial organization promotion factors, and market regulation factors. The results of the study have guiding significance for ensuring the quality and safety of agricultural products, promoting ecological environmental protection, and sustainable agricultural development under the normalization of COVID-19 prevention and control.

Proteomics reveals region-specific hemostatic alterations in response to mechanical ventilation in a preterm lamb model of lung injury.

INTRODUCTION: Preterm infants often require assisted ventilation, however ventilation when applied to the immature lung can initiate ventilator-induced lung injury (VILI). The biotrauma which underscores VILI is largely undefined, and is likely to involve vascular injury responses, including hemostasis. We aimed to use a ventilated, preterm lamb model to: (1) characterize regional alterations in hemostatic mediators within the lung and (2) assess the functional impact of protein alterations on hemostasis by analyzing temporal thrombin generation. MATERIALS AND METHODS: Preterm lambs delivered at 124 to 127 days gestation received 90 min of mechanical ventilation (positive end-expiratory pressure = 8 cm H2O, VT = 6-8 ml/kg) and were compared with unventilated control lambs. At study completion, lung tissue was taken from standardized nondependent and gravity-dependent regions, and Orbitrap-mass spectrometry and KEGG were used to identify and map regional alterations in hemostasis pathway members. Temporal alterations in plasma thrombin generation were assessed. RESULTS: Ventilation was distributed towards the nondependent lung. Significant changes in hemostatic protein abundance, were detected at a two-fold higher rate in the nondependent lung when compared with the gravity-dependent lung. Seven proteins were uniquely altered in non-dependent lung (SERPINA1, MYL12A, RAP1B, RHOA, ITGB1, A2M, GNAI2), compared with a single proteins in gravity-dependent lung (COL1A2). Four proteins were altered in both regions (VTN, FGG, FGA, and ACTB). Tissue protein alterations were mirrored by plasma hypocoagulability at 90-minutes of ventilation. CONCLUSIONS: We observed regionally specific, hemostatic alterations within the preterm lung together with disturbed fibrinolysis following a short period of mechanical ventilation.

Preterm Lung Exhibits Distinct Spatiotemporal Proteome Expression at Initiation of Lung Injury.

The development of regional lung injury in the preterm lung is not well understood. This study aimed to characterize time-dependent and regionally specific injury patterns associated with early ventilation of the preterm lung using a mass spectrometry-based proteomic approach. Preterm lambs delivered at 124-127 days gestation received 15 or 90 minutes of mechanical ventilation (positive end-expiratory pressure = 8 cm H2O, Vt = 6-8 ml/kg) and were compared with unventilated control lambs. At study completion, lung tissue was taken from standardized nondependent and dependent regions, and assessed for lung injury via histology, quantitative PCR, and proteomic analysis using Orbitrap-mass spectrometry. Ingenuity pathway analysis software was used to identify temporal and region-specific enrichments in pathways and functions. Apoptotic cell numbers were ninefold higher in nondependent lung at 15 and 90 minutes compared with controls, whereas proliferative cells were increased fourfold in the dependent lung at 90 minutes. The relative gene expression of lung injury markers was increased at 90 minutes in nondependent lung and unchanged in gravity-dependent lung. Within the proteome, the number of differentially expressed proteins was fourfold higher in the nondependent lung than the dependent lung. The number of differential proteins increased over time in both lung regions. A total of 95% of enriched canonical pathways and 94% of enriched cellular and molecular functions were identified only in nondependent lung tissue from the 90-minute ventilation group. In conclusion, complex injury pathways are initiated within the preterm lung after 15 minutes of ventilation and amplified by continuing ventilation. Injury development is region specific, with greater alterations within the proteome of nondependent lung.