Peroxiredoxin 4 directly affects the male fertility outcome in porcine.

Peroxiredoxins (Prdxs) are known to play a critical role in regulating male fertility as antioxidant enzymes. Although several studies have suggested a close association between Prdxs and male fertility, few studies have explored the efficacy of Prdxs to predict male fertility. Therefore, the current study was designed to discover the most efficient biomarkers among the Prdxs with six isoforms. Our study showed a significant positive correlation between the litter size and the levels of PRDX 4 among all isoforms in spermatozoa. Subsequently, a regression analysis using a combination of markers was conducted to increase efficacy for fertility prediction. Nevertheless, PRDX4 had the highest efficacy compared to other combination models to predict litter size. The prediction accuracy of male fertility was further evaluated through receiver operating characteristic curve analysis, which showed that PRDX 4 could predict the litter size with high overall accuracy of 95%. Moreover, litter size was increased by 1.55 piglets after predicting high litter size using PRDX 4. This is the first study to comprehensively elucidate the role of all isoforms of PRDXs on male fertility to the best of our knowledge. PRDX 4 was tested and evaluated up to a practical level. Data here reported suggesting PRDX 4 marker allowed the highest accuracy for male fertility prediction and diagnosis, leading to a measurable improvement in the male fertility outcome.

Therapeutic protein production in vivo after electroporation-assisted intramuscular gene delivery.

Gene delivery to skeletal muscles assisted by electroporation(EP) is a promising strategy for in vivo production of the therapeutic proteins. But the commonly used procedure required the application of electric pulses with voltages at above 200V/cm and durations at least 40ms, which would result in several damages in the muscle and limited surviving cells expressing transgene. We reported here an optimization study of the various electric pulse parameters to reduce toxicity while maintain transgene expression. In addition, we also found that the secreted transgene product level detected in serum samples may not correlate with the total gene expression level in muscles. Based on our data, we'd propose some less damaging electroporation parameters that may be useful for intramuscular gene delivery and therapeutic protein production.