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Comp Biochem Physiol B ; 88(1): 81-4, 1987.
Article in English | MEDLINE | ID: mdl-3677617

ABSTRACT

1. An extract from the rodent nematode Nippostrongylus brasiliensis contained putative receptors that bound radiolabeled sex hormones, based on isoelectric focusing. 2. Binding of radiolabeled testosterone by receptors at pH 4.4 was highly inhibited by the androgen analogs, testosterone-3-oxime and 4-aza-5-androsten-3-on-17 beta-ol. 3. Binding of radiolabeled progesterone by receptors at pH 6.4 was highly inhibited by the progesterone analogs 3,5-seco-4-norpregnan-5-on-3-oic acid and 19-norethisterone or 21-deoxycorticosterone. 4. Binding of radiolabeled 17 beta-estradiol by receptors at pH 4.9 was highly inhibited by epiandrosterone. 5. In vivo development of N. brasiliensis to the adult was partially inhibited by selected steroid analogs.


Subject(s)
Nippostrongylus/metabolism , Receptors, Androgen/metabolism , Receptors, Estradiol/metabolism , Receptors, Estrogen/metabolism , Receptors, Progesterone/metabolism , Animals , Binding, Competitive , Female , Kinetics , Male , Sex Factors
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