Mithramycin A Improves Functional Recovery by Inhibiting BSCB Disruption and Hemorrhage after Spinal Cord Injury.

After spinal cord injury (SCI), blood-spinal cord barrier (BSCB) disruption and progressive hemorrhage lead to secondary injury, subsequent apoptosis and/or necrosis of neurons and glia, causing permanent neurological deficits. Growing evidence indicates that mithramycin A (MA), an anti-cancer drug, has neuroprotective effects in ischemic brain injury and Huntington's disease (HD). However, the precise mechanism underlying its protective effects is largely unknown. Here, we examined the effect of MA on BSCB breakdown and hemorrhage as well as subsequent inflammation after SCI. After moderate spinal cord contusion injury at T9, MA (150 µg/kg) was immediately injected intraperitoneally (i.p.) and further injected once a day for 5 days. Our data show that MA attenuated BSCB disruption and hemorrhage, and inhibited the infiltration of neutrophils and macrophages after SCI. Consistent with these findings, the expression of inflammatory mediators was significantly alleviated by MA. MA also inhibited the expression and activation of matrix metalloprotease-9 (MMP-9) after injury, which is known to disrupt BSCB and the degradation of tight junction (TJ) proteins. In addition, the expression of sulfonylurea receptor 1 (SUR1) and transient receptor potential melastatin 4 (TRPM4), which are known to mediate hemorrhage at an early stage after SCI, was significantly blocked by MA treatment. Finally, MA inhibited apoptotic cell death and improved functional recovery after injury. Thus, our results demonstrated that MA improves functional recovery by attenuating BSCB disruption and hemorrhage through the downregulation of SUR1/TRPM4 and MMP-9 after SCI.

A simple kinetic analysis of syngas during steam hydrogasification of biomass using a novel inverted batch reactor with instant high pressure feeding.

A newly designed inverted batch reactor equipped with a pressure-driven feeding system was built for investigating the kinetics of syngas during the steam hydrogasification (SHR) of biomass. The system could instantly load the feedstock into the reactor at high temperature and pressure, which simulated the way to transport the feedstock into a hot and pressurized gasifier. Experiments were conducted from 600°C to 700°C. The inverted reactor showed very high heating rate by enhancing the carbon conversion and syngas production. The kinetic study showed that the rates of CH4, CO and CO2 formation during SHR were increased when the gasification temperature went up. SHR had comparatively lower activation energy for CH4 production. The activation energies of CH4, CO and CO2 during SHR were 42.8, 51.8 and 14kJ/mol, respectively.

Digestible and metabolizable energy in corn grains from different origins for growing pigs.

An experiment was conducted to determine the digestible energy (DE) and metabolizable energy (ME) concentrations in nine sources of corn grains fed to growing pigs and to compare the energy values among their countries of origin. A total of nine sources of corn grains including five sources of yellow corn from the United States (USY), two sources of yellow corn from South Africa (SAY), and two sources of white corn from South Africa (SAW) were used. Nine barrows with an initial body weight of 37.1 ± 8.6 kg were allotted to a 9 × 9 Latin square design with nine diets and nine periods. The DE concentration in SAY (3347 kcal/kg) was greater (P < 0.001) than in USY (3269 kcal/kg), but was less (P < 0.001) than in SAW (3436 kcal/kg) on an as-fed basis. Similarly, the ME concentration in SAY (3291 kcal/kg) was greater (P < 0.001) than in USY (3209 kcal/kg), but was less (P < 0.001) than in SAW (3386 kcal/kg). In conclusion, the DE and ME concentrations in nine sources of corn grains are different among their countries of origin.

Relationship between the course of depression symptoms and the left ventricular mass index and left ventricular filling pressure in chronic haemodialysis patients.

AIM: Multiple measurements of depression symptoms over time were more predictive of cardiovascular mortality than a single time measurement performed at baseline. The aim of this study is to evaluate the association of the course of depression symptoms, based on repeated assessments of depression symptoms over time, with left ventricular mass index (LVMI) and left ventricular filling pressure (LVFP) in patients on haemodialysis (HD). METHODS: The level of depression symptoms in 61 patients on HD were prospectively assessed using the Beck Depression Inventory (BDI) at baseline and at three intervals (5, 10, 15 months). Doppler echocardiographic examinations were performed at the end of follow up. RESULTS: At the end of follow up, the patients were divided into three groups according to their course of depression symptoms: non-depression (n = 21), intermittent depression (n = 23) and persistent depression (n = 17). LVMI and LVFP were significantly increased in the persistent depression symptoms group compared to those of the non-depression symptoms group and the intermittent depression symptoms group. Persistent depression symptoms were independently associated with LVMI (ß-coefficient = 0.347, P = 0.017) and LVFP (ß-coefficient = 0.274, P = 0.048) after adjustment for age, sex, systolic blood pressure, diastolic blood pressure, diabetes and interdialytic weight gain. CONCLUSION: In our study, persistent depression symptoms were associated with left ventricular hypertrophy and diastolic dysfunction. Our data may provide a more complete understanding of cardiovascular risk associated with depression symptoms in patients on HD.