ABSTRACT
The Korean endemic Eranthis byunsanensis B.Y. Sun, 1993 (Ranunculaceae) is a rare plant distributed in the southwestern part of the Korean Peninsula. The complete chloroplast (cp) genome of E. byunsanensis was sequenced by next-generation sequencing (NGS) using an Illumina HiSeq X platform. The cp genome of E. byunsanensis is 160,324 bp in length with 37.9% GC content. It showed a typical quadripartite structure consisting of a pair of inverted repeats (IRs; 28,356 bp), a large single-copy region (LSC; 87,671 bp), and a small single-copy region (SSC; 15,941 bp). The cp genome comprises 130 genes including 85 protein-coding genes (PCGs), 37 tRNA genes, and eight rRNA genes. The molecular phylogenetic analysis indicates that E. byunsanensis is closely related to Eranthis stellata, both of which belong to the genus Eranthis.
ABSTRACT
Berchemia racemosa Siebold & Zucc., 1845 is a rare species distributed in restricted areas in the western Korean peninsula. In this study, the complete chloroplast genome (plastome) of B. racemosa was sequenced and assembled by Illumina paired-end sequencing. The plastome of B. racemosa was 161,187 bp in length and was quadripartite in structure, including a large single-copy (LSC) region of 89,503 bp, a small single-copy (SSC) region of 18,214 bp, and two inverted repeats of 26,735 bp. The GC content was 37.2%. The plastome of B. racemosa contains 130 genes, including eight ribosomal RNA (rRNA) genes, 37 transfer RNA (tRNA) genes, and 85 protein-coding genes. Phylogenetic analysis using complete genome sequences showed that B. racemosa is most closely related to Berchemia flavescens.
ABSTRACT
Spergularia marina (L.) Griseb, 1843 (Caryophyllaceae) is a halophytic plant widely distributed along the southwestern coast of the Korean Peninsula. In this study, the complete chloroplast genome sequence of S. marina was determined using next-generation sequencing (NGS). The chloroplast genome of S. marina is 152,460 bp in length with 36.7% GC content. It comprises a large single-copy (LSC; 83,321), a small single copy (SSC; 17,205 bp), and a pair of inverted repeats (IRs; 25,967 bp) with a typical quadripartite structure. It consists of 131 genes, including 86 protein-coding genes, 8 ribosomal RNAs, and 37 transfer RNAs. Phylogenetic analysis using complete chloroplast genomes showed that among the 17 Caryophyllaceae species, S. marina is most closely related to Spergula arvensis. Since no complete chloroplast genome of the genus Spergularia has been reported to date, our study provides useful genetic information for determining phylogenetic relationships within the Caryophyllaceae.
ABSTRACT
Rhodococcus fascians is an important pathogen that infects various herbaceous perennials and reduces their economic value. In this study, we examined R. fascians isolates carrying a virulence gene from symptomatic lily plants grown in South Korea. Phylogenetic analysis using the nucleotide sequences of 16S rRNA, vicA, and fasD led to the classification of the isolates into four different strains of R. fascians. Inoculation of Nicotiana benthamiana with these isolates slowed root growth and resulted in symptoms of leafy gall. These findings elucidate the diversification of domestic pathogenic R. fascians and may lead to an accurate causal diagnosis to help reduce economic losses in the bulb market.
ABSTRACT
The acaricidal activities of major constituents from the oil of Juniperus chinensis (var. globosa) leaves were compared with those of DEET (N,N-diethyl-m-toluamide) by using impregnated fabric disk bioassay against Dermatophagoides spp. and Tyrophagus putrescentiae. Toxicity varied with doses as well as chemical composition. The 50% lethal doses (LD50) of J. chinensis oil against Dermatophagoides farinae, Dermatophagoides pteronyssinus, and T. putrescentiae were 21.60, 19.89, and 38.10 microg/cm2, respectively. The active constituent was purified using silica gel chromatography and high-performance liquid chromatography. The acaricidal component was identified as bomyl acetate through gas chromatography-mass spectrometry, 1H nuclear magnetic resonance (NMR), 13C-NMR, 1H-13C shift correlation spectrum-NMR, and distortionless enhancement by polarization transfer-NMR. The LD50 of bornyl acetate (2.94 microg/cm2) against D. farinae was significantly lower than those of DEET (37.13 microg/cm2) and alpha-eudesmol (29.72 microg/cm2). Similar results were observed when bomyl acetate and alpha-eudesmol were tested against D. pteronyssinus and T. putrescentiae. The lower LD50 of bornyl acetate indicates that it may be responsible for the major acaricidal activity against house dust and stored food mites, even though it constitutes only 19.5% of J. chinensis oil. Overall, these findings indicated that bornyl acetate and c-eudesmol have potential for use as control agents against house dust and stored food mites.