ABSTRACT
BACKGROUND: Running exercise is an effective means to enhance cardiorespiratory fitness and body composition. Besides these health benefits, running is also associated with musculoskeletal injuries that can be more prevalent in individuals with excessive body weight. Little is known regarding the specific effects of overweight and foot pronation on ground reaction force distribution during running. Therefore, this study aimed to investigate the effects of overweight/obesity and foot pronation on running kinetics. METHODS: Eighty-four young adults were allocated to four experimental groups: non-excessive body weight/non-pronated feet; non-excessive body weight/pronated feet; overweight or obesity/ non-pronated feet and overweight or obesity/pronated feet. Biomechanical testing included participants to run at ~ 3.2 m/s over an 18-m walkway with an embedded force plate at its midpoint. Three-dimensional ground reaction forces were recorded and normalized to body mass to evaluate running kinetics from 20 running trials. Test-re-test reliability for running speed data demonstrated ICC > 0.94 for each group and in total. RESULTS: The results indicated significantly lower vertical impact peak forces (p = 0.001, effect size = 0.12), shorter time to reach the vertical impact peak (p = 0.006, effect size = 0.08) and reduced vertical loading rate (p = 0.0007, effect size = 0.13) in individuals with excessive body weight (overweight or obesity/non-pronated feet group and overweight or obesity/pronated feet) compared with individuals non-excessive body weight (non-excessive body weight/non-pronated feet and non-excessive body weight/pronated feet). Moreover, the excessive body weight groups presented lower peak braking (p = 0.01, effect size = 0.06) and propulsion forces (p = 0.003, effect size = 0.09), lower medio-lateral loading rate (p = 0.0009, effect size = 0.12), and greater free moments (p = 0.01, effect size = 0.07) when compared to the non-overweight groups. Moreover, a significant body mass by foot pronation interaction was found for peak medio-lateral loading rate. Non-excessive body weight/pronated feet, excessive body weight/non-pronated feet and excessive body weight/pronation groups presented lower medio-lateral loading rates compared to non-excessive body weight/non-pronated feet (p = 0.0001, effect size = 0.13). CONCLUSIONS: Our results suggest that excessive body weight has an impact on ground reaction forces during running. We particularly noted an increase in medio-lateral and torsional forces during the stance phase. Individuals with excessive body weight appear to adapt their running patterns in an effort to attenuate early vertical impact loading.
ABSTRACT
OBJECTIVE: Three-dimensional (3D) biomimetic nanofiber scaffolds have widespread ap- plications in biomedical tissue engineering. They provide a suitable environment for cel- lular adhesion, survival, proliferation and differentiation, guide new tissue formation and development, and are one of the outstanding goals of tissue engineering. Electrospinning has recently emerged as a leading technique for producing biomimetic scaffolds with mi- cro to nanoscale topography and a high porosity similar to the natural extracellular matrix (ECM). These scaffolds are comprised of synthetic and natural polymers for tissue engi- neering applications. Several kinds of cells such as human embryonic stem cells (hESCs) and mouse ESCs (mESCs) have been cultured and differentiated on nanofiber scaffolds. mESCs can be induced to differentiate into a particular cell lineage when cultured as em- bryoid bodies (EBs) on nano-sized scaffolds. MATERIALS AND METHODS: We cultured mESCs (2500 cells/100 µl) in 96-well plates with knockout Dulbecco's modified eagle medium (DMEM-KO) and Roswell Park Memorial Institute-1640 (RPMI-1640), both supplemented with 20% ESC grade fetal bovine serum (FBS) and essential factors in the presence of leukemia inhibitory factor (LIF). mESCs were seeded at a density of 2500 cells/100 µl onto electrospun polycaprolactone (PCL) nanofibers in 96-well plates. The control group comprised mESCs grown on tissue cul- ture plates (TCP) at a density of 2500 cells/100 µl. Differentiation of mESCs into mouse hematopoietic stem cells (mHSCs) was performed by stem cell factor (SCF), interleukin-3 (IL-3), IL-6 and Fms-related tyrosine kinase ligand (Flt3-L) cytokines for both the PCL and TCP groups. We performed an experimental study of mESCs differentiation. RESULTS: PCL was compared to conventional TCP for survival and differentiation of mESCs to mHSCs. There were significantly more mESCs in the PCL group. Flowcyto- metric analysis revealed differences in hematopoietic differentiation between the PCL and TCP culture systems. There were more CD34+(Sca1+) and CD133+cells subpopulations in the PCL group compared to the conventional TCP culture system. CONCLUSION: The nanofiber scaffold, as an effective surface, improves survival and differentiation of mESCs into mHSCs compared to gelatin coated TCP. More studies are necessary to understand how the topographical features of electrospun fibers af- fect cell growth and behavior. This can be achieved by designing biomimetic scaffolds for tissue engineering.
ABSTRACT
OBJECTIVE: Superparamagnetic iron oxide nanoparticles (SPIONs) have been used to label mammalian cells and to monitor their fate in vivo using magnetic resonance imaging (MRI). However, the effectiveness of phenotype of labeled cells by SPIONs is still a matter of question. The aim of this study was to investigate the efficiency and biological effects of labeled mouse embryonic stem cells (mESCs) using ferumoxide- protamine sulfate complex. MATERIALS AND METHODS: In an experimental study, undifferentiated mESCs, C571 line, a generous gift of Stem Cell Technology Company, were cultured on gelatin-coated flasks. The proliferation and viability of SPION-labeled cells were compared with control. ESCs and embryoid bodies (EBs) derived from differentiated hematopoietic stem cells (HSCs) were analyzed for stage-specific cell surface markers using fluorescence-activated cell sorting (FACS). RESULTS: Our observations showed that SPIONs have no effect on the self-renewal ability of mESCs. Reverse microscopic observations and prussian blue staining revealed 100% of cells were labeled with iron particles. SPION-labeled mESCs did not significantly alter cell viability and proliferation activity. Furthermore, labeling did not alter expression of representative surface phenotypic markers such as stage-specific embryonic antigen 1 (SSEA1) and cluster of differentiation 117 (CD117) on undifferentiated ESC and CD34, CD38 on HSCs, as measured by flowcytometry. CONCLUSION: According to the results of the present study, SPIONs-labeling method as MRI agents in mESCs has no negative effects on growth, morphology, viability, proliferation and differentiation that can be monitored in vivo, noninvasively. Noninvasive cell tracking methods are considered as new perspectives in cell therapy for clinical use and as an easy method for evaluating the placement of stem cells after transplantation.