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[Fragment of the gene encoding chymotrypsin and trypsin inhibitor protein of potato tubers].
Parfenov, I A; Revina, T A; Pashkovskii, P P; Radiukina, N L; Valueva, T A.
Prikl Biokhim Mikrobiol ; 47(4): 402-7, 2011.
Article in Russian | MEDLINE | ID: mdl-21950113

ABSTRACT

Product of polymerase chain reaction designated as PKPIJ-B was isolated after amplification from genomic DNA of potato (Solarium tuberosum L., Zhukov Jubilee cultivar) using the designed primers. Nucleotide sequence of PKPIJ-B was determined and amino acid sequence of protein was restored. Analysis of this sequence has allowed us to suggest that isolated gene fragment encodes chymotrypsin and trypsin inhibitor protein (PKCI-23 potato Kunitz-type chymotrypsin inhibitor) of potato tubers.


Subject(s)
DNA/chemistry , Plant Proteins/chemistry , Plant Tubers/genetics , Protein Isoforms/chemistry , Solanum tuberosum/genetics , Trypsin Inhibitors/chemistry , Amino Acid Sequence , Amino Acid Substitution , Chymotrypsin/metabolism , DNA Primers , Molecular Sequence Data , Phylogeny , Plant Proteins/genetics , Plant Proteins/metabolism , Plant Tubers/chemistry , Plant Tubers/enzymology , Polymorphism, Genetic , Protein Isoforms/genetics , Protein Isoforms/metabolism , Sequence Homology, Amino Acid , Solanum tuberosum/chemistry , Solanum tuberosum/enzymology , Trypsin/metabolism , Trypsin Inhibitors/genetics , Trypsin Inhibitors/metabolism
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