Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
STAR Protoc ; 5(1): 102875, 2024 Mar 15.
Article in English | MEDLINE | ID: mdl-38386547

ABSTRACT

Here, we present a protocol for isolating and culturing mouse photoreceptors in a minimal, chemically defined medium free from serum. We describe steps for retina dissection, enzymatic dissociation, photoreceptor enrichment, cell culture, extracellular vesicles (EVs) enrichment, and EV ultrastructural analysis. This protocol, which has been verified for cultured cells derived from multiple murine strains, allows for the study of several aspects of photoreceptor biology, including EV isolation and nanotube formation. For complete details on the use and execution of this protocol, please refer to Kalargyrou et al. (2021).1.


Subject(s)
Extracellular Vesicles , Retina , Animals , Mice , Cell Culture Techniques , Dissection
SELECTION OF CITATIONS
SEARCH DETAIL