ABSTRACT
We developed a new test system to detect the omicron variant of SARS-CoV-2 using allele-specific reverse transcription PCR and estimated the frequency of its detection in patients living in the Novosibirsk Region. Clinical samples were divided into 3 groups: samples collected from December 1 to December 30, 2021 (group 1; n=66), from December 30, 2021 to January 10, 2022 (group 2; n=20), and from January 11 to January 22, 2022 (group 3; n=101). Based on the identification of 5 mutations specific to SARS-CoV-2 (B.1.1.529), two systems of oligonucleotide primers and probes were developed for detecting this coronavirus genotype in clinical samples. Limit of detection (LOD95) was 4×103 genome equivalents per 1 ml of clinical sample for the first test system and 2×103 for the for the second test system. The omicron variant of SARS-CoV-2 was absent in group 1 of studied samples, but was detected in 20% (4/20) of group 2 samples and 88% of group 2 samples collected within less than 2 weeks of January 2022. Using developed test system, we showed that in less than 2 weeks the omicron variant has become dominant in patients, which confirms previously published data on its exceptional contagiousness.
Subject(s)
COVID-19 , SARS-CoV-2 , COVID-19/diagnosis , Humans , RNA, Viral , SARS-CoV-2/genetics , Sensitivity and SpecificityABSTRACT
Spindle assembly relies on three main classes of microtubules (MTs): MTs nucleated by the centrosomes, MTs nucleated near the chromosomes/kinetochores and MTs nucleated from preexisting MTs through the augmin-based pathway. Here, we review the roles of these microtubule generation pathways in Drosophila spindle assembly. The extant results indicate that female meiotic cells, male meiotic cells, larval brain cells and S2 tissue culture cells exploit specific pathway combinations for generating the MTs necessary for spindle formation. Thus, different Drosophila cell types have specific modes of spindle assembly, which might be related to specific functional and developmental requirements.
