ABSTRACT
OBJECTIVE: The aim of this study was to present results of the post-pandemic phase of A(H1N1)pdm09 virus infection in pregnant women in Ceará, Brazil, during the January-June 2012 influenza season. RESULTS: One hundred and fifty-four nasopharyngeal swab samples were collected from pregnant women admitted to hospitals with suspected severe acute respiratory infection (SARI). Fifty-three (34·4%) had laboratory-confirmed A(H1N1)pdm09 virus infection with 15 (28·3%) outpatients and 38 (71·7%) hospitalized. Five (9·4%) women were in the first trimester of pregnancy, 20 (37·7%) in the second trimester of pregnancy, and 24 (45·2%) in the third trimester of pregnancy. Three had no information about the time of pregnancy. Six samples from newborns were also analyzed, of which three were nasopharyngeal swab positive for A(H1N1)pdm09. These swabs were collected immediately after birth, with the exception of one that was collected on the day after birth. CONCLUSION: Our findings suggest that transplacental transfer of influenza viruses could occur as a result of severe illness in pregnancy. It is therefore important to encourage women to be vaccinated against influenza in order to avoid pregnancy complications.
ABSTRACT
BACKGROUND: Characterization of the human respiratory syncytial virus (HRSV) season at the local level has important implications for appropriate decisions on the time period for administration of specific prophylaxis. OBJECTIVES: (1) To describe five consecutive epidemic periods of HRSV in an equatorial city of Brazil and (2) to show preliminary data on genomic diversity of circulating HRSV. PATIENTS/METHODS: Nasopharyngeal aspirates of 2885 children attending the emergency room and wards of a public hospital were collected and screened by indirect immunofluorescence for HRSV infections during five consecutive years (from January 2004 to December 2008). In addition, the genetic and antigenic variability of the HRSV strains isolated was evaluated by partial nucleotide sequencing of the protein G gene. RESULTS: HRSV was detected in 15·8% of the analyzed samples. HRSV seasons occurred in a restricted period of each year. The onset of each HRSV season was variable (February to May), but the end always occurred in July. From the 456 HRSV infections found, 86 cases with bronchiolitis were genotyped. Both HRSV subgroups (A and B) cocirculated during the five epidemic periods. The 58 HRSV-A strains grouped into two clades, GA2 and GA5. In respect of the HRSV-B strains, the 28 samples grouped into two clades: GB3 and BA. CONCLUSIONS: HRSV accounts for a substantial proportion of ARI in the study population. As in temperate countries, HRSV infections in this equatorial area of Brazil also cause seasonal yearly epidemics, and this has implications for prophylaxis strategies. The city of Fortaleza follows the same worldwide trend of circulation of genotypes of HRSV.
Subject(s)
Epidemics , Respiratory Syncytial Virus Infections/epidemiology , Respiratory Syncytial Virus, Human/isolation & purification , Adolescent , Brazil/epidemiology , Child , Child, Preschool , Cluster Analysis , Female , Genetic Variation , Humans , Infant , Infant, Newborn , Male , Molecular Sequence Data , Nasopharynx/virology , Phylogeny , RNA, Viral/genetics , Respiratory Syncytial Virus, Human/classification , Respiratory Syncytial Virus, Human/genetics , Seasons , Sequence Analysis, DNA , Viral Envelope Proteins/geneticsABSTRACT
Influenza epidemics occur worldwide annually. The incidence of influenza shows a seasonal pattern in temperate areas, but little is known about influenza seasonality in tropical regions. The objective of this study was to determine the prevalence and the seasonal pattern of influenza infections in children living in the city of Fortaleza in northeastern Brazil. An indirect immunofluorescence assay was performed on nasopharyngeal aspirates collected from children attending in ambulatories, emergency rooms, and wards of the Hospital Infantil Albert Sabin with suspicion of acute respiratory infection during 7 consecutive years (2001-2007). Influenza viruses were detected in 6.3% (234/3,708) of specimens. Laboratory-based surveillance data showed a clear annual epidemic cycle of influenza, with a peak usually occurring in the rainy periods. In Fortaleza, flu infections occurred at a low level throughout the year but exhibit a marked seasonal increase during the rainy season.
