Resistance Profile of Bovine Mastitis Isolates, Presence of the mecA Gene and Identification of ESBL Producing Strains from Small Rural Dairy Properties.

Bovine mastitis is an inflammation of the mammary gland in response to invasion by opportunistic agents. Due to the high economic importance of dairy production and the complexity related to animal health, the objective of this work was to identify and evaluate the antibacterial resistance profile of samples of mastitis milk, milking hand and milking equipment from small rural dairy farms belonging to the northwest region of the state of Paraná, Brazil. Five small, non-technical dairy farms in the municipalities of Boa Esperança, Juranda and Tapejara, all belonging to the northwest region of the state of Paraná, Brazil, were selected. The properties had Holstein and/or crossbred herds, carried out a bucket-by-foot milking system and all had the presence of animals with subclinical mastitis confirmed by the California Mastitis Test. Samples of sterile swabs from the milking insufflators, the milking hand and milk samples were collected-and later, isolation tests and phenotypic characterization of the samples, sensitivity tests to antimicrobials and phenotypic tests for the detection of beta-producing strains were performed with extended-spectrum beta-lactamase (ESBL), molecular identification of Staphylococcus aureus isolates and mecA gene research. Of the 199 samples collected from the 15 selected properties in the municipalities of Boa Esperança, Tapejara and Juranda, 72 (36.20%) were classified as multiresistant. Isolated from milkers' hands and milking machines, which phenotypically produce extended-spectrum beta-lactamase (ESBL), the presence of the mecA gene was also observed in 11 isolates of Staphylococcus spp. of milk samples, machines and milking hands. Mastitis can be spread to the herd through the milking process by the milkers' instruments and hands, and adequate management measures can prevent its transmission and the conscious use of antibiotics decreases the prevalence of multidrug-resistant pathogens. In this work, different pathogenic bacteria were detected in mastitic milk, milking equipment and milking hand with a high percentage (36.20%) of isolates classified as multidrug resistant. In addition, the presence phenotypically (ESBL) and molecularly (mecA gene) of isolates carrying resistance genes was also verified. These results directly reflect on the health of the animals, the health of the workers and the health of the respective environment, which can enable the continuity of the propagation of the etiological agents involved in the mastitis infection. The awareness of producers and workers on these properties about the disease, transmission, sanitary aspects and adequate management and treatment are essential for improving milk production and production efficiency.

Periodontite em crianças portadoras da síndrome de Papillon-Lefèvre: revista da literatura / Periodontitis in children with Papillon-Lefèvre Syndrome: literature review

A periodontite é uma das várias manifestações patológicas produzidas pela síndrome de Papillon-Lefèvre. Assim, reconhecendo que a periodontite de acometimento precoce pode levar à mortalidade dentária e suas seqüelas indesejáveis, é fundamental que o odontopediatra estabeleça um diagnóstico em tempo hábil e implemente um tratamento preventivo e/ou curativo em crianças portadoras de tal síndrome. Desta forma, o presente estudo apresenta através de revisão de literatura, as manifestações clínicas e sistêmicas da síndrome de Papillon-Lefèvre e repercussões para a cavidade bucal, facilitando o diagnóstico e elaboração de atitudes preventivas e terapêuticas

Doenças periodontais destrutivas na dentadura decídua: I. Periodontite associada à neutropenia / Destructive periodontal diseases in the primary dentition: I. Periodontitis associated with neutropenia

As doenças periodontais na dentadura decídua ainda são pouco estudadas e esclarecidas, principalmente as de características destrutivas. Enquanto a gengivite é freqüentemente relatada, a periodontite é tratada de forma tangencial. Dentre as doenças periodontais relevantes na Odontopediatria, destaca-se a periodontite associada à neutropenia. Nessa linha de racioncínio, o presente artigo apresenta o conceito da neutropenia e sua relação com as seqüelas indesejáveis ao periodonto do paciente infantil, tais como, perdas ósseas extensas e generalizadas, retração gengival, formação de bolsas periodontais e conseqüente perda precoce de dentes decíduos. Frente a essas considerações, é fundamental que o odontopediatra tenha conhecimento dessas alterações, para que se possam incluir esses conceitos em seus exames clínicos de rotina, bem como estabelecer um plano de tratamento preventivo e curativo, visando o bem-estar no contexto da saúde geral da criança