ABSTRACT
To verify the influence of ozone (O3) therapy on an experimental model of rheumatoid arthritis (RA), 30 male Wistar rats were randomly allocated to 2 groups, control (C) and treatment (T), and subdivided into control (C12, C48, C72) and treatment (T12, T48, T72) groups. RA was induced by administration of collagenase plus complete Freud's adjuvant in the knee joint region. The animals were treated with ozone therapy (1 ml O3 injection in the knee i.a.) according to group assignment: T12, 2 h; T48, 2 and 24 h; and T72, 2, 24, and 48 h post-RA induction. The different animal groups were euthanized 12, 24, or 72 h post-RA induction, respectively. Synovial exudate levels of IL-10, IL-12p70, TNF-α, INF-γ, and MCP-1 were assessed by flow cytometry, and histopathological analysis of the knee cartilage was conducted. Ozone therapy effectively decreases inflammation, reducing IL-12 and TNF-α, and increasing IL10. O3 did not statistically affect INF-γ or MCP-1 levels. More expressive results were obtained with group T72, i.e., treated 2, 24, and 48 h post-RA induction, which indicates that longer-term ozone treatment is more effective than a single acute application. Ozone therapy effectively reduced inflammation with effects, at least in part, mediated through reduction of pro-inflammatory cytokines and activation of IL-10 anti-inflammatory cytokine.
Subject(s)
Anti-Inflammatory Agents/administration & dosage , Arthritis, Experimental/metabolism , Arthritis, Experimental/therapy , Inflammation Mediators/antagonists & inhibitors , Inflammation Mediators/metabolism , Ozone/administration & dosage , Animals , Arthritis, Experimental/chemically induced , Disease Models, Animal , Freund's Adjuvant/toxicity , Male , Oxidants, Photochemical/administration & dosage , Rats , Rats, WistarABSTRACT
ABSTRACT Alzheimer's is a neurodegenerative disease characterized by cognitive impairment commonly associated with mood disorders, which trigger depressive reactions and compromise mental performance and functionality. The objective of the present study was to verify the effects of L-tryptophan and to analyze motor behavior in an experimental model of depression caused by the Alzheimer's disease process. The sample consisted of 40 Wistar rats divided equally into two groups, 20 animals treated with L-tryptophan and 20 control animals. Both groups received spatial memory training in the water maze task and were submitted to stereotaxic surgery to induce dementia. The treated group achieved better spatial memory activity than the control group in the Morris water maze. Treatment with L-tryptophan had beneficial effects on reactive memory.
RESUMO O Alzheimer é uma doença neurodegenerativa caracterizado pelo comprometimento cognitivo comumente associado a transtornos do humor, os quais desencadeiam reações depressivas, comprometem o desempenho mental e a funcionalidade. O objetivo do presente estudo foi verificar os efeitos do L-triptofano e analisar o comportamento motor em modelo experimental com depressão decorrente do processo de Alzheimer. A amostra foi composta por 40 ratos da linhagem wistar divididos igualmente em dois grupos, 20 animais tratados com L-triptofano e 20 animais pertencentes ao grupo controle. Ambos os grupos receberam treinamento da memória espacial no later water maze e foram submetidos à cirurgia estereotáxica para indução demencial. Verificou-se através do labirinto aquático de Morris que o grupo tratado obteve atividade para memória espacial melhor do que o grupo controle. O tratamento com L-triptofano demonstrou melhor benefício na memória reativa.
Subject(s)
Humans , Physical Education and Training , Tryptophan , Self Efficacy , Depression , Alzheimer Disease , Intellectual Disability , Teaching , FacultyABSTRACT
Introdução: O acidente vascular cerebral é a doença neurológica que mais frequentemente acomete o sistema nervoso e é a principal causa de incapacidades físicas e mentais. Objetivo: Avaliar o comportamento motor em modelos de animais após cirurgia de oclusão da carótida comum. Método: Foram utilizados 16 ratos Wistars, e foi realizado cirurgia anteriormente na cervical para exposição da carótida comum, sendo obstruída por pinça hemostática. A amostra foi dividida em dois grupos, GI e GII Resultados: Na análise estatística, os valores da média e desvio-padrão para a reação normal foi de 42,5±0,70 e para a reação de flexão 0,0±0,0 para o Grupo I. Para o grupo II, a média e o desvio-padrão para reação normal foi de 36,0±1,41 e para a reação de flexão, de 20,0±2,82. Conclusão: A atividade física é essencial para o dia a dia em pacientes portadores de lesão neurológica, proporcionando melhora locomotora.
Introduction: The stroke is a neurological disease that most commonly affects the nervous system and is the leading cause of mental and physical disabilities. Objective: To evaluate motor behavior in animal models after coronary occlusion of the common carotid. Method: 16 Wistar rats, and surgery was performed earlier in the neck to expose the common carotid artery, being obstructed by a hemostat. The sample was divided into two groups GI and GII Results: Statistical analysis, the mean and standard deviation for the normal reaction was 42.5 ± 0.70 and for the reaction of flexion 0.0 ± 0, 0 for Group I. In group II, the mean and standard deviation for normal reaction was 36.0 ± 1.41 and for the reaction of flexion, 20.0 ± 2.82. Conclusion: Physical activity is essential for daily life in patients with neurological injury and improved the locomotor activity.
Subject(s)
Animals , Male , Rats , Stroke/surgery , Stroke Rehabilitation , Exercise , Cross-Sectional Studies , Rats, Wistar , Stroke/complicationsABSTRACT
Discutem-se alguns dos principais aspectos que envolvem o idoso, as transformações acontecidas durante o processo do envelhecimento no campo biológico e social e sua relação como participante na Universidade Aberta da Terceira Idade da Unicentro.
Subject(s)
Aged , Aging , GeriatricsABSTRACT
Discutem-se alguns dos principais aspectos que envolvem o idoso, as transformações acontecidas durante o processo do envelhecimento no campo biológico e social e sua relação como participante na Universidade Aberta da Terceira Idade da Unicentro