ABSTRACT
OBJECTIVE: To analyze the impact of moderate physical exercise on the total and differential leukocyte counts and red blood cell count of 36 sixty-day-old adult male Wistar rats subjected to early malnourishment. METHODS: The rats were divided in nourished (N - casein 17%) and malnourished groups (M - casein 8%) and thesegroups were then subdivided in trained (T) untrained (U) creating four groups NT, NU, MT and MU. The NT and MTgroups were submitted to moderate physical exercise using a treadmill (60 min/day, 5 days/week for 8 weeks). Onthe 1st day, before the training started T0 and 24 hours after the last training day of the week (T1 until T8), a 1 mLaliquot of blood was collected from the animals' tails for analysis. The total leukocyte count was evaluated in a cellcounter with an electronic microscope. The cyanmethemoglobin technique was used to measure the hemoglobin level. The hematocrit values were determined as a percentage using the micro-hematocrit technique with a microcapillaryreader and a cell counter was used to determine the red blood cell count. The t-test was used for statistical analysis and a p-value < 0.05 was considered significant. Data are expressed as means ± standard deviation. RESULTS: There was a significant difference in the total leukocyte count between the NT (9.1 ± 0.1) and MT groups (8.0 ± 0.1) from T1 and in neutrophils between the NT (22.1 ± 0.6) and MT groups (24.6 ± 1.8) from T7 (p < 0.05). There was no statistical significance in the hemoglobin, hematocrit and red blood cell count from T1. CONCLUSIONS: According to the results of this study, moderate physical exercise seems to have induced physiologic adaptation in adult rats from T1.
ABSTRACT
OBJECTIVE: To analyze the impact of moderate physical exercise on the total and differential leukocyte counts and red blood cell count of 36 sixty-day-old adult male Wistar rats subjected to early malnourishment. METHODS: The rats were divided in nourished (N - casein 17%) and malnourished groups (M - casein 8%) and thesegroups were then subdivided in trained (T) untrained (U) creating four groups NT, NU, MT and MU. The NT and MTgroups were submitted to moderate physical exercise using a treadmill (60 min/day, 5 days/week for 8 weeks). Onthe 1st day, before the training started T0 and 24 hours after the last training day of the week (T1 until T8), a 1 mLaliquot of blood was collected from the animals' tails for analysis. The total leukocyte count was evaluated in a cellcounter with an electronic microscope. The cyanmethemoglobin technique was used to measure the hemoglobin level. The hematocrit values were determined as a percentage using the micro-hematocrit technique with a microcapillaryreader and a cell counter was used to determine the red blood cell count. The t-test was used for statistical analysis and a p-value < 0.05 was considered significant. Data are expressed as means ± standard deviation. RESULTS: There was a significant difference in the total leukocyte count between the NT (9.1 ± 0.1) and MT groups (8.0 ± 0.1) from T1 and in neutrophils between the NT (22.1 ± 0.6) and MT groups (24.6 ± 1.8) from T7 (p < 0.05). There was no statistical significance in the hemoglobin, hematocrit and red blood cell count from T1. CONCLUSIONS: According to the results of this study, moderate physical exercise seems to have induced physiologic adaptation in adult rats from T1.
Subject(s)
Animals , Rats , Exercise , Malnutrition , Physical Exertion , LeukocytesABSTRACT
Este estudo teve o objetivo de propor modelos estatísticos preditores dos níveis de pressão arterial sistólica e diastólica, em individuos do sexo masculino e feminino. Para as análises de regressão, foram selecionadas variáveis: idade (ID), frequência cardíaca de repouso (FCr), estatura (EST), sedentarismo (DS), não sedentarismo (NS), perímetros da cintura (CT), do quadril (QD), massa corporal total (MCT) para ambos os sexos e dobras cutâneas para homens: abdominal (AB), triciptal(TR) e subescapular (SE) e para mulheres: abdominal (AB), supraíliaca(SI) e coxa(CX), Utilisou-se o processo de Stepwise para selecionar as variáveis significativas aos modelos de regressão linear múltipla, com fator de transformação de Box e Cox(1964) simplificado, (p<0,05). Obteve-se uma alta correlação das variáveis: FCr,EST, CT, QD, MCT, para ambos os sexos e a ID só para o feminino. Os modelos estatísticos propostos, inferem sobre a necessidade de considerar as variáveis acima citadas, sobre as reais medidas de pressão arterial em indivíduos normotensos de ambos os sexos