Effects of increased chronic loading on articular cartilage material properties in the lapine tibio-femoral joint.

Methods of producing relevant and quantifiable load alterations in vivo with which to study load-induced cartilage degeneration analogous to osteoarthritis are limited. An animal model was used to investigate the effects of increased chronic loads on articular cartilage. Mature rabbits were randomized into one of three experimentally loaded groups and a fourth unoperated control group. A mechanical-loading device was skeletally fixed to the hind limb of animals in the loaded groups. Engaging the device resulted in an additional load of 0%, +22% or +44% body weight to the medial compartment of the experimental knee, while allowing normal joint function. Following a 12-week loading protocol, a creep-indentation test and needle probe test were used to determine the biphasic material properties and thickness of the cartilage at four locations of each femoral and tibial condyle of the experimental and contralateral limbs. Analyses of covariance were performed to compare outcome measures across the treatment groups. The effect of increased load was site and load-level specific with alterations of material properties and thickness most prominent in the posterior region of the medial compartment of the tibia. At this site, permeability increased 128% and thickness increased 28% in the +44% body weight group relative to the 0% body weight group. This model of altered chronic loading initiated changes in the material properties to the articular cartilage at the sites of increased load over 12-weeks that were consistent with early degenerative changes suggesting that increased tibio-femoral loading may be responsible for the alterations. This work begins to elucidate the chronic-load threshold and the time course of cartilage degeneration at different levels of altered loading.

The effect of coordinate system choice and segment reference on RSA-based knee translation measures.

Roentgen stereophotogrammetric analysis (RSA) can be utilized to accurately describe joint kinematics, but even when measuring small displacements within radiographically discernible structures, standardized reference frames are imperative for useful comparison across patients and across studies. In the current paper, accurately controlled laboratory models demonstrated the considerable influence that a mere 1.9-cm offset of the origin of the coordinate system from the rotation axes could exert on translation measures when rotations were occurring. In addition, the use of two different coordinate systems to gauge translation on a radiographic anterior-posterior (A-P) knee laxity exam resulted in a significant correlation (R(2)=0.562) between the two systems; however, differences of up 9.28 mm were found between corresponding measurements. This implies that clinical conclusions can potentially be upheld or refuted, based on the same data set, subject to coordinate system definition. Although the data analyzed presently involved the knee joint, similar issues surround the RSA motion analysis of other joints as well.

Material properties of articular cartilage in the rabbit tibial plateau.

The material properties of articular cartilage in the rabbit tibial plateau were determined using biphasic indentation creep tests. Cartilage specimens from matched-pair hind limbs of rabbits approximately 4 months of age and greater than 12 months of age were tested on two locations within each compartment using a custom built materials testing apparatus. A three-way ANOVA was used to determine the effect of leg, compartment, and test location on the material properties (aggregate modulus, permeability, and Poisson's ratio) and thickness of the cartilage for each set of specimens. While no differences were observed in cartilage properties between the left and right legs, differences between compartments were found in each set of specimens. For cartilage from the adolescent group, values for aggregate modulus were 40% less in the medial compartment compared to the lateral compartment, while values for permeability and thickness were greater in the medial compartment compared to the lateral compartment (57% and 30%, respectively). Values for Poisson's ratio were 19% less in the medial compartment compared to the lateral compartment. There was also a strong trend for thickness to differ between test locations. Similar findings were observed for cartilage from the mature group with values for permeability and thickness being greater in the medial compartment compared to the lateral compartment (66% and 34%, respectively). Values for Poisson's ratio were 22% less in the medial compartment compared to the lateral compartment.

In vivo loads in the medial compartment of the rabbit knee.

BACKGROUND: The compressive loads produced in the medial compartment of a rabbit knee during hopping are unknown, and necessary for the development of an animal model to study unicompartmental osteoarthritis. METHODS: In an effort to obtain such data, a surgical procedure was developed to insert a pressure sensor into the medial tibial compartment of a New Zealand white rabbit knee joint, and an inactive sensor of the same thickness into the lateral tibial compartment. After the rabbits recovered from anesthesia, contact pressure was measured as the rabbits hopped. FINDINGS: The average peak pressure in the medial compartment was 60 N/cm2 (SD 16), a value that corresponds to 39% of the animals' body weight (range: 21-61%, SD 11%). INTERPRETATION: This is the first report of measured in vivo pressures associated with the load transmitted through the medial compartment of the skeletally mature New Zealand White rabbit, a model frequently used to study the progression of osteoarthritis.

Ankle eversion torque response to sudden ankle inversion Torque response in unbraced, braced, and pre-activated situations.

In 13 young ankle stable subjects, ankle eversion torque and peroneal EMG were simultaneously recorded in response to sudden ankle inversion. The eversion torque response was bi-phasic. The initial development of torque, which was responsible for 30% of the maximal eversion torque response, was observed 135 ms after the start of platform rotation and correlated well with the onset of the automatic postural peroneal EMG response. The remaining eversion torque response commenced after 305 ms, strongly correlating with the onset of the peroneal long latency voluntary EMG activity. With the ankle unbraced, 66% of the maximal torque level was reached in 326 ms. While braced, the same torque magnitude was reached using 230 ms (p<0.02), and pre-activation of the peroneal muscles allowed the subjects to reach the same level of torque in 89 ms (p<0.0005). Prior to the study, a common reaction pattern to sudden inversion was expected in an ankle stable population, but review of the eversion torque and EMG data from the 13 subjects revealed three different voluntary reaction patterns: 10 subjects showed an efficient activation of evertor muscles; two subjects stiffened their ankles with activation of both in- and evertor muscles; and one subject showed a marginal voluntary activation of the ankle evertors. The results of the study indicate that the reaction to sudden ankle inversion is not solely automatic. The main part of the torque response is voluntarily mediated and inter-individual differences in strategy seem to exist in healthy subjects.

Strain on the anterior cruciate ligament during closed kinetic chain exercises.

PURPOSE: The purpose of this investigation was to characterize the ACL strains produced during four commonly prescribed CKC exercises; the step-up, the step-down, the lunge, and the one-legged sit to stand. We hypothesized that the ACL strains produced during the lunge and one-legged sit to stand exercises (the exercises that challenge the leg musculature to a greater extent and utilize greater hip flexion) would be less than those produced during the step-up and step-down exercises. METHODS: The strains in the anteromedial bundle of the ACL were measured while nine subjects, who had normal ligaments, performed the four exercises. Peak ACL strain values and the ACL strain patterns as a function of knee flexion angle were compared between exercises. RESULTS: No significant differences were found between the peak ACL strain values (mean +/- SEM) between exercises (step-up: 2.5 +/- 0.36; step-down: 2.6 +/- 0.34; lunge 1.9 +/- 0.50; one-legged sit to stand: 2.8 +/- 0.27). The mean ACL strain values as a function of knee flexion angle were not significantly different. On average, there was a significant increase in ACL strain as the knee was extended for each exercise. CONCLUSIONS: The ACL strain responses produced during these CKC exercises were equal and similar to those produced during other rehabilitation exercises (i.e., squatting, active extension of the knee) previously tested.

The effects of compressive load and knee joint torque on peak anterior cruciate ligament strains.

BACKGROUND: High graft strains incurred during rehabilitation after anterior cruciate ligament reconstruction may be minimized if an external compressive load is simultaneously applied to the joint during closed kinetic chain exercises. HYPOTHESES: Peak anterior cruciate ligament strains will 1). increase with an increase in resistance torque during an exercise that involves concentric contraction of the extensor mechanism, 2). decrease with an increase in resistance torque during an exercise that involves concentric contraction of the flexors, and 3). decrease when an external compressive load is applied to the knee during both exercises relative to the no external compressive load condition. STUDY DESIGN: Controlled laboratory study. METHODS: Strains in the anteromedial bundle were measured in 10 subjects with normal ligaments. Flexor and extensor exercises were performed against controlled resistance torques with and without a compressive load applied to the foot. RESULTS: An increase in resistance produced an increase in peak anterior cruciate ligament strain for the extensor exercise with no compressive load applied. During the flexor exercise without a compressive load, an increase in resistance produced a decrease in peak strains. During the extensor exercise, the peak anterior cruciate ligament strain was not reduced with the application of the external compressive force. CONCLUSIONS: Extensor and flexor exercises that incorporate an external compressive load do not shield the anterior cruciate ligament from strain. However, no additional increase in strain occurs with an increase in resistance when the external compressive load is applied. Thus, it may be possible to increase the activity of the quadriceps muscles without increasing the strain by applying a compressive load (as with closed kinetic chain exercises).

Measurement of anterior-posterior knee laxity: a comparison of three techniques.

Several non-invasive techniques have been developed to assess anterior-posterior (A-P) laxity of the knee, however, their accuracy remains unclear. Roentgen Stereophotogrammetry Analysis (RSA) is a three-dimensional motion analysis method that has been shown to be an accurate tool for evaluating joint kinematics. Thus. RSA provides a means that can be used to evaluate other less invasive techniques. The objectives of this study were to compare A-P laxity values as measured using the KT-1000 Knee Arthrometer, planar stress radiography and RSA, and to determine if they detect similar changes in A-P laxity over time in 15 subjects following anterior cruciate ligament reconstruction with patellar tendon autografts. The A-P laxity values of the injured knee were measured immediately following surgery and at their 3-, 6-, and 12-month follow-up visits. A-P laxity was defined as the A-P translation of the tibia relative to the femur that occurred between the posterior and anterior shear load limits of -90 to +130 N, respectively. The values of A-P laxity across all time points were 11.4 +/- 3.0, 10.2 +/- 3.3, and 6.9 +/- 3.0 mm (mean +/- standard deviation) for the KT-1000, planar stress radiography and the RSA methods, respectively. These values were significantly different from each other (p < 0.001). The two-dimensional analyses techniques (planar stress radiography and the KT-1000) consistently over-estimated the true laxity values that were obtained using the three-dimensional RSA technique. Significant increases in A-P laxity values over time were also detected with the KT-1000 (p = 0.04) and the RSA technique (p = 0.04). However, this increase was not evident when using planar stress radiography (p = 0.89). This study determined that the KT-1000 and RSA document temporal changes in A-P laxity following ACL reconstruction that were not documented by planar stress radiography.