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1.
Plant Cell ; 36(5): 1655-1672, 2024 May 01.
Article in English | MEDLINE | ID: mdl-38242840

ABSTRACT

SUPPRESSOR OF MAX2 (SMAX)1-LIKE (SMXL) proteins are a plant-specific clade of type I HSP100/Clp-ATPases. SMXL genes are present in virtually all land plant genomes. However, they have mainly been studied in angiosperms. In Arabidopsis (Arabidopsis thaliana), 3 functional SMXL subclades have been identified: SMAX1/SMXL2, SMXL345, and SMXL678. Of these, 2 subclades ensure endogenous phytohormone signal transduction. SMAX1/SMXL2 proteins are involved in KAI2 ligand (KL) signaling, while SMXL678 proteins are involved in strigolactone (SL) signaling. Many questions remain regarding the mode of action of these proteins, as well as their ancestral roles. We addressed these questions by investigating the functions of the 4 SMXL genes in the moss Physcomitrium patens. We demonstrate that PpSMXL proteins are involved in the conserved ancestral MAX2-dependent KL signaling pathway and negatively regulate growth. However, PpSMXL proteins expressed in Arabidopsis cannot replace SMAX1 or SMXL2 function in KL signaling, whereas they can functionally replace SMXL4 and SMXL5 and restore root growth. Therefore, the molecular functions of SMXL proteins are conserved, but their interaction networks are not. Moreover, the PpSMXLC/D clade positively regulates SL signal transduction in P. patens. Overall, our data reveal that SMXL proteins in moss mediate crosstalk between the SL and KL signaling pathways.


Subject(s)
Arabidopsis Proteins , Bryopsida , Gene Expression Regulation, Plant , Plant Proteins , Bryopsida/genetics , Bryopsida/growth & development , Bryopsida/metabolism , Plant Proteins/genetics , Plant Proteins/metabolism , Arabidopsis Proteins/metabolism , Arabidopsis Proteins/genetics , Arabidopsis/genetics , Arabidopsis/metabolism , Arabidopsis/growth & development , Signal Transduction , Phylogeny , Lactones/metabolism
2.
J Nat Prod ; 85(8): 1976-1992, 2022 08 26.
Article in English | MEDLINE | ID: mdl-35776904

ABSTRACT

Strigolactones (SLs) are plant hormones exuded in the rhizosphere with a signaling role for the development of arbuscular mycorrhizal (AM) fungi and as stimulants of seed germination of the parasitic weeds Orobanche, Phelipanche, and Striga, the most threatening weeds of major crops worldwide. Phelipanche ramosa is present mainly on rape, hemp, and tobacco in France. P. ramosa 2a preferentially attacks hemp, while P. ramosa 1 attacks rapeseed. The recently isolated cannalactone (14) from hemp root exudates has been characterized as a noncanonical SL that selectively stimulates the germination of P. ramosa 2a seeds in comparison with P. ramosa 1. In the present work, (-)-solanacol (5), a canonical orobanchol-type SL exuded by tobacco and tomato, was established to possess a remarkable selective germination stimulant activity for P. ramosa 2a seeds. Two cannalactone analogues, named (±)-SdL19 and (±)-SdL118, have been synthesized. They have an unsaturated acyclic carbon chain with a tertiary hydroxy group and a methyl or a cyclopropyl group instead of a cyclohexane A-ring, respectively. (±)-SdL analogues are able to selectively stimulate P. ramosa 2a, revealing that these minimal structural elements are key for this selective bioactivity. In addition, (±)-SdL19 is able to inhibit shoot branching in Pisum sativum and Arabidopsis thaliana and induces hyphal branching in the AM fungus Rhizophagus irregularis, like SLs.


Subject(s)
Arabidopsis , Mycorrhizae , Orobanchaceae , Orobanche , Striga , Germination , Heterocyclic Compounds, 3-Ring , Lactones/chemistry , Lactones/pharmacology , Plant Roots/chemistry , Plant Weeds , Seeds
3.
Front Plant Sci ; 13: 887347, 2022.
Article in English | MEDLINE | ID: mdl-35720613

ABSTRACT

Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (Striga, Orobanche, and Phelipanche) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clavé (GC) probes and performed structure-activity relationship studies on pea, Arabidopsis thaliana, and Physcomitrium (formerly Physcomitrella) patens. The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants.

4.
Commun Biol ; 5(1): 126, 2022 02 11.
Article in English | MEDLINE | ID: mdl-35149763

ABSTRACT

KAI2 proteins are plant α/ß hydrolase receptors which perceive smoke-derived butenolide signals and endogenous, yet unidentified KAI2-ligands (KLs). The number of functional KAI2 receptors varies among species and KAI2 gene duplication and sub-functionalization likely plays an adaptative role by altering specificity towards different KLs. Legumes represent one of the largest families of flowering plants and contain many agronomic crops. Prior to their diversification, KAI2 underwent duplication resulting in KAI2A and KAI2B. Here we demonstrate that Pisum sativum KAI2A and KAI2B are active receptors and enzymes with divergent ligand stereoselectivity. KAI2B has a higher affinity for and hydrolyses a broader range of substrates including strigolactone-like stereoisomers. We determine the crystal structures of PsKAI2B in apo and butenolide-bound states. The biochemical, structural, and mass spectra analyses of KAI2s reveal a transient intermediate on the catalytic serine and a stable adduct on the catalytic histidine, confirming its role as a bona fide enzyme. Our work uncovers the stereoselectivity of ligand perception and catalysis by diverged KAI2 receptors and proposes adaptive sensitivity to KAR/KL and strigolactones by KAI2B.


Subject(s)
Arabidopsis Proteins , Arabidopsis , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Catalysis , Pisum sativum/genetics , Pisum sativum/metabolism , Perception , Plant Growth Regulators/genetics
5.
New Phytol ; 232(5): 1909-1916, 2021 12.
Article in English | MEDLINE | ID: mdl-34498760

ABSTRACT

The timing of leaf emergence at the shoot apical meristem, or plastochron, is highly regulated in plants. Among the genes known to regulate the plastochron in Arabidopsis (Arabidopsis thaliana), KLUH (KLU), orthologous to the rice (Oryza sativa) PLASTOCHRON1, encodes the cytochrome P450 CYP78A5, and is thought to act through generation of a still unknown mobile signal. As klu mutants display not only a short plastochron but also a branching phenotype reminiscent of strigolactone (SL) mutants, we investigated whether KLU/CYP78A5 is involved in SL biosynthesis. We combined a genetic approach, a parasitic plant seed germination bioassay to test klu root exudates, and analysis of transcript abundances of SL-biosynthesis genes in the Arabidopsis klu mutants. We demonstrate that KLU is not involved in the SL-biosynthesis pathway. Moreover, this work allowed us to uncover a new role for SL during Arabidopsis development in modulating plastochron via a KLU-dependent pathway. Globally our data reveal that KLU is required for plastochron-specific SL responses, a first indication of crosstalk between SL and the KLU-derived signal.


Subject(s)
Arabidopsis Proteins , Arabidopsis , Arabidopsis/genetics , Arabidopsis/metabolism , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Gene Expression Regulation, Plant , Heterocyclic Compounds, 3-Ring , Lactones
6.
Plant J ; 107(6): 1756-1770, 2021 09.
Article in English | MEDLINE | ID: mdl-34245626

ABSTRACT

DWARF53 (D53) in rice (Oryza sativa) and its homologs in Arabidopsis (Arabidopsis thaliana), SUPPRESSOR OF MAX2-LIKE 6 (SMXL6), SMXL7 and SMXL8, are well established negative regulators of strigolactone (SL) signalling in shoot branching regulation. Little is known of pea (Pisum sativum) homologs and whether D53 and related SMXLs are specific to SL signalling pathways. Here, we identify two allelic pea mutants, dormant3 (dor3), and demonstrate through gene mapping and sequencing that DOR3 corresponds to a homolog of D53 and SMXL6/SMXL7, designated PsSMXL7. Phenotype analysis, gene expression, protein and hormone quantification assays were performed to determine the role of PsSMXL7 in regulation of bud outgrowth and the role of PsSMXL7 and D53 in integrating SL and cytokinin (CK) responses. Like D53 and related SMXLs, we show that PsSMXL7 can be degraded by SL and induces feedback upregulation of PsSMXL7 transcript. Here we reveal a system conserved in pea and rice, whereby CK also upregulates PsSMXL7/D53 transcripts, providing a clear mechanism for SL and CK cross-talk in the regulation of branching. To further deepen our understanding of the branching network in pea, we provide evidence that SL acts via PsSMXL7 to modulate auxin content via PsAFB5, which itself regulates expression of SL biosynthesis genes. We therefore show that PsSMXL7 is key to a triple hormone network involving an auxin-SL feedback mechanism and SL-CK cross-talk.


Subject(s)
Heterocyclic Compounds, 3-Ring/metabolism , Lactones/metabolism , Pisum sativum/growth & development , Plant Proteins/genetics , Arabidopsis/genetics , Arabidopsis/metabolism , Co-Repressor Proteins/genetics , Co-Repressor Proteins/metabolism , Cytokinins/metabolism , Feedback, Physiological , Gene Expression Regulation, Plant , Indoleacetic Acids/metabolism , Loss of Function Mutation , Oryza , Pisum sativum/genetics , Pisum sativum/metabolism , Phylogeny , Plant Proteins/metabolism , Plants, Genetically Modified , Signal Transduction/genetics
7.
Methods Mol Biol ; 2309: 115-127, 2021.
Article in English | MEDLINE | ID: mdl-34028683

ABSTRACT

Shoot branching is a highly variable trait that evolves during plant development and is influenced by environmental and endogenous cues such as hormones. In particular, strigolactones (SLs) are hormones that play a key role in the control of shoot branching. Branch primordia, axillary buds formed in the leaf axils, display differential growth depending on their position in the plant and also respond to hormone signaling. In this chapter, we will describe how to quantify the degree of shoot branching in two plant model species, Arabidopsis and pea, commonly used to decipher the control of this complex trait. We will also propose several methods to perform treatments of SL or SL analogs, to investigate their bioactivity and effect on the shoot branching patterns of plants of different genotypes.


Subject(s)
Arabidopsis/drug effects , Biological Assay , Heterocyclic Compounds, 3-Ring/pharmacology , Lactones/pharmacology , Pisum sativum/drug effects , Plant Development/drug effects , Plant Growth Regulators/pharmacology , Plant Shoots/drug effects , Plants, Genetically Modified/drug effects , Arabidopsis/genetics , Arabidopsis/growth & development , Hydroponics , Mutation , Pisum sativum/genetics , Pisum sativum/growth & development , Phenotype , Plant Shoots/genetics , Plant Shoots/growth & development , Plants, Genetically Modified/genetics , Plants, Genetically Modified/growth & development
8.
Phytochemistry ; 168: 112112, 2019 Dec.
Article in English | MEDLINE | ID: mdl-31499274

ABSTRACT

Strigolactone (SL) plant hormones control plant architecture and are key players in both symbiotic and parasitic interactions. GR24, a synthetic SL analog, is the worldwide reference compound used in all bioassays for investigating the role of SLs in plant development and in rhizospheric interactions. In 2012, the first characterization of the SL receptor reported the detection of an unknown compound after incubation of GR24 samples with the SL receptor. We reveal here the origin of this compound (P270), which comes from a by-product formed during GR24 chemical synthesis. We present the identification of this by-product, named contalactone. A proposed chemical pathway for its formation is provided as well as an evaluation of its bioactivity on pea, Arabidopsis, root parasitic plant seeds and AM fungi, characterizing it as a SL mimic. Quality of GR24 samples can be easily checked by carrying out microscale hydrolysis in a basic aqueous medium to easily detect P270 as indicator of the presence of the contalactone impurity. In all cases, before being used for bioassays, GR24 must be careful purified by preparative HPLC.


Subject(s)
Arabidopsis/chemistry , Heterocyclic Compounds, 3-Ring/analysis , Lactones/analysis , Chromatography, High Pressure Liquid , Drug Contamination , Heterocyclic Compounds, 3-Ring/chemical synthesis , Lactones/chemical synthesis , Molecular Structure
9.
Phytochem Anal ; 29(1): 59-68, 2018 Jan.
Article in English | MEDLINE | ID: mdl-28851101

ABSTRACT

INTRODUCTION: Strigolactones (SLs) are important plant hormones. They are difficult to analyse because they occur in very small concentrations especially in comparison with other plant hormones and other substances can interfere with their detection. OBJECTIVE: To develop a procedure for the extraction, purification and quantification of SLs from plant roots. METHODOLOGY: Samples were prepared by extraction of plant root tissues with ethyl acetate. Then the extracts were further purified with silica column chromatography. The natural SLs in the final extracts were quantified using novel deuterium labelled SLs. The results of the methodology were compared with those of the procedure of Yoneyama and coworkers. RESULTS: This procedure required about 1-g root samples to detect and quantify simultaneously the SLs (orobanchyl acetate and fabacyl acetate) concentration with high reliability. CONCLUSION: A method was developed for determining endogenous fabacyl acetate and orobanchyl acetate in plant tissue based on novel deuterium labelled standards. A method of orobanchol quantification using a synthetic SL GR24 as internal standard was proposed. Copyright © 2017 John Wiley & Sons, Ltd.


Subject(s)
Chromatography, Liquid/methods , Deuterium , Isotope Labeling , Lactones/chemistry , Spectrometry, Mass, Electrospray Ionization/methods , Tandem Mass Spectrometry/methods , Pisum sativum/chemistry , Plant Roots/chemistry , Reproducibility of Results
10.
PLoS Genet ; 13(12): e1007089, 2017 Dec.
Article in English | MEDLINE | ID: mdl-29220348

ABSTRACT

Strigolactones (SLs) are well known for their role in repressing shoot branching. In pea, increased transcript levels of SL biosynthesis genes are observed in stems of highly branched SL deficient (ramosus1 (rms1) and rms5) and SL response (rms3 and rms4) mutants indicative of negative feedback control. In contrast, the highly branched rms2 mutant has reduced transcript levels of SL biosynthesis genes. Grafting studies and hormone quantification led to a model where RMS2 mediates a shoot-to-root feedback signal that regulates both SL biosynthesis gene transcript levels and xylem sap levels of cytokinin exported from roots. Here we cloned RMS2 using synteny with Medicago truncatula and demonstrated that it encodes a putative auxin receptor of the AFB4/5 clade. Phenotypes similar to rms2 were found in Arabidopsis afb4/5 mutants, including increased shoot branching, low expression of SL biosynthesis genes and high auxin levels in stems. Moreover, afb4/5 and rms2 display a specific resistance to the herbicide picloram. Yeast-two-hybrid experiments supported the hypothesis that the RMS2 protein functions as an auxin receptor. SL root feeding using hydroponics repressed auxin levels in stems and down-regulated transcript levels of auxin biosynthesis genes within one hour. This auxin down-regulation was also observed in plants treated with the polar auxin transport inhibitor NPA. Together these data suggest a homeostatic feedback loop in which auxin up-regulates SL synthesis in an RMS2-dependent manner and SL down-regulates auxin synthesis in an RMS3 and RMS4-dependent manner.


Subject(s)
Arabidopsis Proteins/genetics , Pisum sativum/genetics , Plant Proteins/genetics , Receptors, Cell Surface/genetics , Arabidopsis/genetics , Arabidopsis Proteins/metabolism , Gene Expression Regulation, Plant/drug effects , Gene Expression Regulation, Plant/genetics , Indoleacetic Acids/metabolism , Medicago truncatula/genetics , Pisum sativum/growth & development , Picloram/pharmacology , Plant Growth Regulators/genetics , Plant Growth Regulators/metabolism , Plant Proteins/metabolism , Plant Shoots/drug effects , Plant Shoots/genetics , Plant Shoots/growth & development , Receptors, Cell Surface/metabolism , Signal Transduction
11.
Nat Chem Biol ; 12(10): 787-794, 2016 10.
Article in English | MEDLINE | ID: mdl-27479744

ABSTRACT

Strigolactone plant hormones control plant architecture and are key players in both symbiotic and parasitic interactions. They contain an ABC tricyclic lactone connected to a butenolide group, the D ring. The DWARF14 (D14) strigolactone receptor belongs to the superfamily of α/ß-hydrolases, and is known to hydrolyze the bond between the ABC lactone and the D ring. Here we characterized the binding and catalytic functions of RAMOSUS3 (RMS3), the pea (Pisum sativum) ortholog of rice (Oryza sativa) D14 strigolactone receptor. Using new profluorescent probes with strigolactone-like bioactivity, we found that RMS3 acts as a single-turnover enzyme that explains its apparent low enzymatic rate. We demonstrated the formation of a covalent RMS3-D-ring complex, essential for bioactivity, in which the D ring was attached to histidine 247 of the catalytic triad. These results reveal an undescribed mechanism of plant hormone reception in which the receptor performs an irreversible enzymatic reaction to generate its own ligand.


Subject(s)
4-Butyrolactone/analogs & derivatives , Heterocyclic Compounds, 3-Ring/metabolism , Histidine/metabolism , Lactones/metabolism , Pisum sativum/metabolism , Plant Growth Regulators/metabolism , Plant Proteins/metabolism , 4-Butyrolactone/chemistry , 4-Butyrolactone/metabolism , Histidine/chemistry , Ligands , Molecular Structure , Pisum sativum/enzymology , Plant Growth Regulators/chemistry , Plant Proteins/chemistry
12.
Mol Plant ; 7(4): 675-90, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24249726

ABSTRACT

Strigolactones (SLs) are known not only as plant hormones, but also as rhizosphere signals for establishing symbiotic and parasitic interactions. The design of new specific SL analogs is a challenging goal in understanding the basic plant biology and is also useful to control plant architectures without favoring the development of parasitic plants. Two different molecules (23 (3'-methyl-GR24), 31 (thia-3'-methyl-debranone-like molecule)) already described, and a new one (AR36), for which the synthesis is presented, are biologically compared with the well-known GR24 and the recently identified CISA-1. These different structures emphasize the wide range of parts attached to the D-ring for the bioactivity as a plant hormone. These new compounds possess a common dimethylbutenolide motif but their structure varies in the ABC part of the molecules: 23 has the same ABC part as GR24, while 31 and AR36 carry, respectively, an aromatic ring and an acyclic carbon chain. Detailed information is given for the bioactivity of such derivatives in strigolactone synthesis or in perception mutant plants (pea rms1 and rms4, Arabidopsis max2 and, max4) for different hormonal functions along with their action in the rhizosphere on arbuscular mycorrhizal hyphal growth and parasitic weed germination.


Subject(s)
Lactones/metabolism , Plant Growth Regulators/metabolism , Rhizosphere , Arabidopsis/metabolism , Gene Expression Regulation, Plant , Glomeromycota/metabolism
13.
Plant Physiol ; 163(2): 1012-25, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23943865

ABSTRACT

Strigolactone (SL) mutants in diverse species show reduced stature in addition to their extensive branching. Here, we show that this dwarfism in pea (Pisum sativum) is not attributable to the strong branching of the mutants. The continuous supply of the synthetic SL GR24 via the root system using hydroponics can restore internode length of the SL-deficient rms1 mutant but not of the SL-response rms4 mutant, indicating that SLs stimulate internode elongation via RMS4. Cytological analysis of internode epidermal cells indicates that SLs control cell number but not cell length, suggesting that SL may affect stem elongation by stimulating cell division. Consequently, SLs can repress (in axillary buds) or promote (in the stem) cell division in a tissue-dependent manner. Because gibberellins (GAs) increase internode length by affecting both cell division and cell length, we tested if SLs stimulate internode elongation by affecting GA metabolism or signaling. Genetic analyses using SL-deficient and GA-deficient or DELLA-deficient double mutants, together with molecular and physiological approaches, suggest that SLs act independently from GAs to stimulate internode elongation.


Subject(s)
Gibberellins/metabolism , Lactones/pharmacology , Pisum sativum/growth & development , Plant Shoots/growth & development , Arabidopsis/drug effects , Arabidopsis/metabolism , Cell Count , Cell Size/drug effects , Flowers/drug effects , Flowers/growth & development , Hydroponics , Meristem/drug effects , Meristem/metabolism , Models, Biological , Molecular Sequence Data , Mutation/genetics , Pisum sativum/anatomy & histology , Pisum sativum/drug effects , Pisum sativum/metabolism , Phenotype , Plant Proteins/metabolism , Plant Roots/drug effects , Plant Roots/physiology , Plant Shoots/anatomy & histology , Plant Shoots/drug effects , Plant Stems/cytology , Plant Stems/drug effects , Plant Stems/growth & development , Proteolysis/drug effects
14.
Chemistry ; 19(15): 4849-57, 2013 Apr 08.
Article in English | MEDLINE | ID: mdl-23420702

ABSTRACT

A new general access to A-ring aromatic strigolactones, a new class of plant hormones, has been developed. The key transformations include in sequence ring-closing metathesis, enzymatic kinetic resolution and a radical cyclization with atom transfer to install the tricyclic ABC-ring system. The activity as plant hormones for the inhibition of shoot branching in pea of various analogues synthesized by this strategy is reported.


Subject(s)
Lactones/chemical synthesis , Pisum sativum/chemistry , Plant Growth Regulators/chemical synthesis , Lactones/chemistry , Lactones/metabolism , Molecular Structure , Pisum sativum/metabolism , Plant Growth Regulators/chemistry , Plant Growth Regulators/metabolism , Structure-Activity Relationship
15.
Plant Physiol ; 159(4): 1524-44, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22723084

ABSTRACT

Initially known for their role in the rhizosphere in stimulating the seed germination of parasitic weeds such as the Striga and Orobanche species, and later as host recognition signals for arbuscular mycorrhizal fungi, strigolactones (SLs) were recently rediscovered as a new class of plant hormones involved in the control of shoot branching in plants. Herein, we report the synthesis of new SL analogs and, to our knowledge, the first study of SL structure-activity relationships for their hormonal activity in garden pea (Pisum sativum). Comparisons with their action for the germination of broomrape (Phelipanche ramosa) are also presented. The pea rms1 SL-deficient mutant was used in a SL bioassay based on axillary bud length after direct SL application on the bud. This assay was compared with an assay where SLs were fed via the roots using hydroponics and with a molecular assay in which transcript levels of BRANCHED1, the pea homolog of the maize TEOSINTE BRANCHED1 gene were quantified in axillary buds only 6 h after application of SLs. We have demonstrated that the presence of a Michael acceptor and a methylbutenolide or dimethylbutenolide motif in the same molecule is essential. It was established that the more active analog 23 with a dimethylbutenolide as the D-ring could be used to control the plant architecture without strongly favoring the germination of P. ramosa seeds. Bold numerals refer to numbers of compounds.


Subject(s)
Lactones/chemistry , Lactones/pharmacology , Morphogenesis/drug effects , Pisum sativum/drug effects , Pisum sativum/growth & development , Plant Shoots/drug effects , Plant Shoots/growth & development , Biological Assay , Gene Expression Regulation, Plant/drug effects , Hydroponics , Lactones/chemical synthesis , Models, Biological , Oxidation-Reduction/drug effects , Pisum sativum/genetics , Pisum sativum/metabolism , Plant Proteins/genetics , Plant Proteins/metabolism , Plant Shoots/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Stereoisomerism , Structure-Activity Relationship
16.
Plant Physiol ; 158(1): 225-38, 2012 Jan.
Article in English | MEDLINE | ID: mdl-22045922

ABSTRACT

The function of PsBRC1, the pea (Pisum sativum) homolog of the maize (Zea mays) TEOSINTE BRANCHED1 and the Arabidopsis (Arabidopsis thaliana) BRANCHED1 (AtBRC1) genes, was investigated. The pea Psbrc1 mutant displays an increased shoot-branching phenotype, is able to synthesize strigolactone (SL), and does not respond to SL application. The level of pleiotropy of the SL-deficient ramosus1 (rms1) mutant is higher than in the Psbrc1 mutant, rms1 exhibiting a relatively dwarf phenotype and more extensive branching at upper nodes. The PsBRC1 gene is mostly expressed in the axillary bud and is transcriptionally up-regulated by direct application of the synthetic SL GR24 and down-regulated by the cytokinin (CK) 6-benzylaminopurine. The results suggest that PsBRC1 may have a role in integrating SL and CK signals and that SLs act directly within the bud to regulate its outgrowth. However, the Psbrc1 mutant responds to 6-benzylaminopurine application and decapitation by increasing axillary bud length, implicating a PsBRC1-independent component of the CK response in sustained bud growth. In contrast to other SL-related mutants, the Psbrc1 mutation does not cause a decrease in the CK zeatin riboside in the xylem sap or a strong increase in RMS1 transcript levels, suggesting that the RMS2-dependent feedback is not activated in this mutant. Surprisingly, the double rms1 Psbrc1 mutant displays a strong increase in numbers of branches at cotyledonary nodes, whereas branching at upper nodes is not significantly higher than the branching in rms1. This phenotype indicates a localized regulation of branching at these nodes specific to pea.


Subject(s)
Lactones/metabolism , Pisum sativum/growth & development , Plant Shoots/growth & development , Transcription Factors/genetics , Transcription Factors/metabolism , Arabidopsis Proteins/metabolism , Benzyl Compounds , Cytokinins/genetics , Cytokinins/metabolism , Gene Expression Regulation, Plant , Isopentenyladenosine/analogs & derivatives , Isopentenyladenosine/metabolism , Kinetin/pharmacology , Molecular Sequence Data , Mutation , Pisum sativum/drug effects , Pisum sativum/genetics , Pisum sativum/metabolism , Plant Growth Regulators/pharmacology , Plant Proteins/genetics , Plant Proteins/metabolism , Plant Shoots/genetics , Plant Shoots/metabolism , Purines , Signal Transduction/genetics , Up-Regulation , Xylem/genetics , Xylem/metabolism
17.
Langmuir ; 25(10): 5526-35, 2009 May 19.
Article in English | MEDLINE | ID: mdl-19378931

ABSTRACT

Novel glycidyl-terminated organosilicon coupling agents possessing a trialkoxysilyl head group and a very long hydrocarbon chain (C22) were synthesized. Their ability to afford densely packed self-assembled monolayers (SAMs) grafted on silica-based surfaces was investigated. Transmission FT-IR spectra showed that the most regular films were obtained by using trichloracetic acid as the catalyst (10 M%). Atomic force microscopy (AFM) and optical ellipsometry were consistent with well ordered monolayers exhibiting a marked decrease of the surface roughness. Epifluorescence microscopy revealed that these SAMs possessed a better surface reactivity than monolayers obtained with the commercially available (3-glycidoxypropyl) trimethoxysilane (GPTS) upon grafting of a fluorescent probe (dansylcadaverin). Moreover, direct attachment of fluorescent antibodies (RAG-TRITC) through covalent binding led to higher mean fluorescence intensities, showing that these new SAMs possess high potential for the immobilization of biological molecules.


Subject(s)
Cross-Linking Reagents/chemistry , Membranes, Artificial , Silanes/chemistry , Silicon Dioxide/chemistry , Cadaverine/analogs & derivatives , Cadaverine/chemistry , Catalysis , Fluorescent Dyes/chemistry , Microscopy, Atomic Force , Spectroscopy, Fourier Transform Infrared/methods , Surface Properties
18.
Nature ; 455(7210): 189-94, 2008 Sep 11.
Article in English | MEDLINE | ID: mdl-18690209

ABSTRACT

A carotenoid-derived hormonal signal that inhibits shoot branching in plants has long escaped identification. Strigolactones are compounds thought to be derived from carotenoids and are known to trigger the germination of parasitic plant seeds and stimulate symbiotic fungi. Here we present evidence that carotenoid cleavage dioxygenase 8 shoot branching mutants of pea are strigolactone deficient and that strigolactone application restores the wild-type branching phenotype to ccd8 mutants. Moreover, we show that other branching mutants previously characterized as lacking a response to the branching inhibition signal also lack strigolactone response, and are not deficient in strigolactones. These responses are conserved in Arabidopsis. In agreement with the expected properties of the hormonal signal, exogenous strigolactone can be transported in shoots and act at low concentrations. We suggest that endogenous strigolactones or related compounds inhibit shoot branching in plants. Furthermore, ccd8 mutants demonstrate the diverse effects of strigolactones in shoot branching, mycorrhizal symbiosis and parasitic weed interaction.


Subject(s)
Lactones/metabolism , Pisum sativum/metabolism , Plant Growth Regulators/metabolism , Plant Shoots/growth & development , Plant Shoots/metabolism , Arabidopsis/drug effects , Arabidopsis/genetics , Arabidopsis/growth & development , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Dioxygenases , Genes, Plant/genetics , Lactones/analysis , Lactones/chemistry , Lactones/pharmacology , Mutation , Mycorrhizae/physiology , Oxygenases/genetics , Oxygenases/metabolism , Pisum sativum/drug effects , Pisum sativum/growth & development , Pisum sativum/parasitology , Phenotype , Plant Growth Regulators/analysis , Plant Growth Regulators/chemistry , Plant Growth Regulators/pharmacology , Plant Proteins/genetics , Plant Proteins/metabolism , Plant Roots/drug effects , Plant Roots/metabolism , Plant Shoots/drug effects , Plant Shoots/parasitology , Symbiosis , Terpenes/analysis , Terpenes/chemistry , Terpenes/metabolism , Terpenes/pharmacology
19.
Biosens Bioelectron ; 22(9-10): 2145-50, 2007 Apr 15.
Article in English | MEDLINE | ID: mdl-17097870

ABSTRACT

The efficiency of a monomolecular film of (3-glycidoxypropyl) trimethoxysilane (GPTS) on a shear horizontal guided (Love) acoustic wave immunosensor to detect whole Escherichia coli (E. coli) bacteria is demonstrated. Direct anti-E. coli antibodies grafting onto the sensor surface did not lead to a significant bacteria immobilisation, partially attributed to the SiO2 sensor surface roughness. An innovative method has been set up to get around this difficulty and to detect whole bacteria. It consists in grafting goat anti-mouse antibodies (GAM) onto the sensor surface in a first step and introducing E. coli bacteria mixed with anti-E. coli antibodies onto the sensor in a second step. We describe the characteristics of such a technique like sample preparation time (lower than 30 min) and temperature improvements. A 37 degrees C experimental temperature led to the fastest bacteria binding kinetic, reducing the total analysis time. This method enables to keep the specificity of the antibody/antigen interaction and provides significant results in less than 1h. This leads to a detection threshold of 10(6) bacteria/ml in a 500 microl chamber.


Subject(s)
Biosensing Techniques/instrumentation , Escherichia coli/isolation & purification , Animals , Escherichia coli/immunology , Immunoassay/instrumentation , Mice
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