Explore the Mechanism of Jiawei Shengji Yuhong Ointment in Promoting Wound Healing after Anal Fistula of Damp-heat Type Based on NF-κB/VEGF Signaling Pathway / 浙江中医药大学学报

[Objective]To discuss the effect of Jiawei Shengji Yuhong Ointment on wound healing of rat model after anal fistula operation and its internal mechanism.[Methods]Seventy SD rats were randomly divided into blank group,Vaseline group(model group),Kangfuxin liquor group(control group),Jiawei Shengji Yuhong Ointment group(experimental group),ammonium pyrrolidine dithiocarbamate(PDTC)+vaseline group(PDTC+model group),PDTC+Kangfuxin liquid group(PDTC+control group),PDTC+Jiawei Shengji Yuhong Ointment group(PDTC+experimental group).The damp-heat wound model of rats after anal fistula operation was established and treated with different drugs.At the same time,PDTC intervention group was given intraperitoneal injection of nuclear factor-κB(NF-κB)signaling pathway inhibitor PDTC for 5 consecutive days on the basis of drug intervention.The wound healing of rats in each group was recorded,and the wound healing rate of rats in each group was calculated.On the 3rd,7th and 14th days,the granulation tissues of rats in each group were taken for Western blot and immunohistochemistry to detect the protein expression of NF-κB p65.The expression of interleukin-1β(IL-1β)and vascular endothelial growth factor(VEGF)was detected by enzyme-linked immunosorbent assay(ELISA).Hematoxylin-eosin(HE)staining was used for histomorphological observation.[Results]Compared with other groups,the experimental group and PDTC+experimental group could significantly shorten the wound healing time,and the wound healing rates of PDTC+experimental group and experimental group were higher(P＜0.05).HE staining results also confirmed that PDTC+experimental group can effectively promote the rapid growth of granulation tissue.The results of immunohistochemistry and Western blot showed that was inhibited significantly in experimental group and PDTC+experimental group the protein expression of NF-κB p65 in rat wound tissue,and the difference was statistically significant compared with the other groups(P＜0.05).The results of ELISA showed that the content of IL-1 β in each group decreased gradually at 3,7 and 14 d after treatment,and the content of IL-1[3 in PDTC+experimental group was the lowest(P＜0.05).The content of VEGF in each group increased gradually,and the content of VEGF in PDTC+experimental group and experimental group was the highest(P＜0.05).[Conclusion]Jiawei Shengji Yuhong Ointment can inhibit the expression of NF-κB p65 and IL-1 β protein and up-regulate the expression of VEGF protein through NF-κB signaling pathway,thereby promoting the angiogenesis of vascular endothelial cells,reducing inflammation,and promoting the wound healing after anal fistula surgery.

Clinical research progress in Traditional Chinese Medicine in treating wound healing after anal fistula surgery.

In the study of the mechanism of wound healing after anal fistula surgery, how to scientifically and efficiently promote wound healing is of great significance. At present, modern medical treatment of wounds after anal fistula surgery mostly focuses on physical therapy intervention, new wound dressing and packing, and external application of growth factors. However, these therapies have many problems, and there is still no consensus on their clinical use. Traditional Chinese Medicine (TCM) has several methods to promote wound healing, such as oral administration, rubbing, and fumigation, which have a long history and obvious efficacy, but research in this area is relatively scattered and lacks classification and summarizing. Therefore, this paper analyzes and summarizes the existing research on TCM for promotion of wound healing after anal fistula surgery, carries out targeted analyses according to different clinical syndromes and treatment methods, and analyzes the defects in current research and anticipates future research trends in order to provide theoretical support for the advantages of TCM in promoting wound healing after anal fistula surgery.

Exploration on specialty construction of nursing specialty (elderly nursing direction) based on 1+X certificate system / 中国实用护理杂志

Objective:To explore the "Trinity" professional construction path of 1 + X elderly care and 1+ X dementia elderly care and nursing (elderly nursing direction), solve the problem that the three are difficult to be effectively compatible in the process of professional talent training, and cultivate more compound technical talents with "one specialty and multiple abilities" to meet the needs of aging society.Methods:In June 2021, 50 students from class 1 of 2020 nursing major (elderly nursing direction) of Pingxiang health vocational college were selected as the experimental group and 50 students from class 2 were selected as the control group. The nursing students in the experimental group were taught and trained by the newly revised talent training scheme, curriculum standards and a series of corresponding teaching reform measures. The students in the control group were trained by the conventional mode. After the course of the third semester, the passing rate of 1+X elderly care, the passing rate of 1+ X dementia elderly care and the score of geriatric nursing core competence evaluation scale were compared and analyzed between the two groups.Results:The total score of the test group was 156.60 ± 6.00 and that of the control group was 140.86 ± 9.20. The difference was statistically significant ( t=10.13, P<0.05). The passing rate of 1+ X elderly care in the experimental group was 96.43%(27/28), and that of 1 + X elderly care in the control group was 11/15. The difference was statistically significant ( χ2=5.07, P<0.05). The passing rate of care for the elderly with 1 + X dementia was 95.45%(21/22), and the passing rate of care for the elderly with 1 + X dementia was 8/13, the difference was statistically significant ( χ2=4.45, P<0.05). Conclusions:Based on the "Trinity" professional construction mode of nursing specialty (geriatric nursing direction) under the 1 + X certificate system, it can improve students′ core competence of geriatric nursing, improve the passing rate of vocational skill level certificate, and provide reference for the professional construction of nursing specialty (geriatric nursing direction) in medical and health higher vocational colleges.