ABSTRACT
Cytokines are thought to regulate prostaglandin (PG) secretion in the bovine endometrium. However, there is no consensus about the role of interleukin-1alpha (IL1A) on PG secretion. The objective of this study was to examine the influence of IL1A on basal and interferon-tau (IFNT)-regulated PG in vitro secretion, as well its effects on PG secretion, progesterone (P(4)) output, and corpus luteum (CL) in vivo lifespan. Explants of bovine endometrium (days 16-17 of the estrous cycle or early pregnancy) were stimulated with IL1A (10 ng/ml), IFNT (30 ng/ml), or IL1A combined with IFN. IL1A alone strongly stimulated luteotrophic PGE(2) secretion by endometrial tissues of both pregnant and nonpregnant cows. IL1A also stimulated luteolytic PGF(2alpha) output in the late luteal phase. IFNT augmented the stimulatory effect of IL1A on PGE(2) secretion. In an in vivo experiment, saline or IL1A at different doses (0.001-10 microg/per animal) was applied to the uterine lumen on day 16 of the cycle. Only the highest dose of IL1A caused a temporal increase in PGF(2alpha) secretion, while it had no effect on P(4) secretion or CL lifespan. Application of 0.1 and 1 microg IL1A stimulated P(4) and PGE(2) output and prolonged the CL lifespan. Although IL1A may stimulate in vitro luteolytic PGF(2alpha) secretion during the estrous cycle, it only acts as a luteotrophic factor in vivo. IL1A increased luteotrophic PGE(2) and P(4) output, inhibiting spontaneous luteolysis. These luteotrophic effects may result in appropriate luteal development and function in cows during the estrous cycle and early pregnancy.
Subject(s)
Cattle/physiology , Corpus Luteum/drug effects , Interleukin-1alpha/pharmacology , Luteolytic Agents/pharmacology , Pregnancy, Animal , Animals , Cells, Cultured , Corpus Luteum/physiology , Dose-Response Relationship, Drug , Endometrium/drug effects , Endometrium/metabolism , Estrous Cycle/drug effects , Estrous Cycle/metabolism , Female , Insemination, Artificial , Interferon Type I/pharmacology , Luteolysis/drug effects , Male , Pregnancy , Pregnancy Proteins/pharmacology , Pregnancy, Animal/drug effects , Prostaglandins/metabolismABSTRACT
The aim of this study was to examine whether active metabolites of phytoestrogens (equol and para-ethyl-phenol) inhibit sensitivity of bovine corpus luteum (CL) to luteinizing hormone (LH) and to auto/paracrine luteotropic factors (prostaglandin E2-PGE2 and prostaglandin F(2alpha)-PGF(2alpha)), and whether they influence pulsatile progesterone (P4) secretion by the bovine CL. In in vivo experiments, high levels of equol and para-ethyl-phenol were found in plasma and in the CL tissue of heifers and cows fed a soy bean diet (2.5 kg/animal/day), along with lower concentrations of P4 (P < 0.05). Both Prostaglandins (PG) and LH strongly stimulated P4 secretion in cultured pieces of CL that were collected from cows fed a standard diet (P < 0.01). There was no effect of PGs and LH on P4 stimulation in CLs obtained from cows fed a diet rich in soy bean. Finally, we examined whether active metabolites of phytoestrogens participated in regulation of pulsatile P4 secretion and LH-stimulated P4 secretion in vitro using a microdialysis system. Equol and para-ethyl-phenol had no effect on basic and pulsatile P4 secretion in CLs during 240 min of perfusion when compared to the control (P < 0.05). However, they inhibited LH-stimulated P4 secretion (P < 0.05). Phytoestrogens and their metabolites may disrupt CL function by inhibiting PG- and LH-stimulated P4 secretion.
