Elucidating the chemical profile and biological studies of Verbascum diversifolium Hochst. extracts.

The present study was designed to evaluate the chemical composition, antioxidant, enzyme inhibition and cytotoxic properties of different extracts from aerial parts of V. diversifolium (family Scrophulariaceae), a plant that is native to Lebanon, Syria and Turkey. Six extracts, namely, hexane, dichloromethane (DCM), ethyl acetate (EtOAc), ethanol (EtOH), 70% EtOH, and water (aqueous) were prepared by maceration. The EtOH extract was predominated by the presence of rutin (4280.20 µg g-1) and p-coumaric acid (3044.01 µg g-1) while the highest accumulation of kaempferol-3-glucoside (1537.38 µg g-1), caffeic acid (130.13 µg g-1) and 4-hydroxy benzoic acid (465.93 µg g-1) was recorded in the 70% EtOH, aqueous, and EtOAc extracts, respectively. The EtOH (46.86 mg TE/g) and 70% EtOH (46.33 mg TE/g) extracts displayed the highest DPPH radical scavenging result. Both these extracts, along with the aqueous one, exerted the highest ABTS radical scavenging result (73.03-73.56 mg TE/g). The EtOH and 70% EtOH extracts revealed the most potent anti-AChE (2.66 and 2.64 mg GALAE/g) and anti-glucosidase (1.07 and 1.09 mmol ACAE/g) activities. The aqueous extract was the most efficacious in inhibiting the proliferation of prostate cancer (DU-145) cells with an IC50 of 8.71 µg/mL and a Selectivity Index of 3.7. In conclusion, this study appraised the use of V. diversifolium aerial parts as a potential therapeutic source for future development of phytopharmaceuticals that target specific oxidative stress-linked diseases including diabetes, cancer, cardiovascular disease, and Alzheimer's disease among others.

Exploring Sorbus torminalis Leaves: Unveiling a Promising Natural Resource for Diverse Chemical and Biological Applications.

Sorbus torminalis (L.) Crantz has a rich history of versatile applications spanning the fields of medicine and nutrition. It is noteworthy that the decoction obtained from S. torminalis leaves is a traditional treatment method against both diabetes and stomach disorders. Phytochemical profiling determined by HPLC/MS-MS. The effects of the extracts on cell viability were investigated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method against MDA-MB-231 cell line (human breast adenocarcinoma).The ethanol/water extract contained more concentration of total phenolic (91.41 mg gallic acid (GAE) equivalent /gr) and flavanoid (29.10 mg rutin (RE) equivalent/gr) in the tested extract (p<0.05). Resulting of HPLC analysis, the chemical constituents varied depending on the solvents and chlorogenic acid, hyperoside, isoquercetin, delphindin-3,5-diglucoside, procyanidin B2, epicatechin, neochlorogenic acid, 3,5-dicaffeoylquinic acid were identified in all extracts. Overall, ethanol, n-hexane and ethyl acetate extracts showed the highest inhibition for the tyrosinase enzyme. The effect of leaf extracts of S. torminalis on antimicrobial, biofilm inhibitory, and anticancer activities was examined. Based on outcomes of our study recognize this plant as a critical source of medically active chemicals for feasible phytopharmaceutical and nutraceutical applications, providing the first scientific insight into the detailed biological and chemical profiles of S. torminalis.

Chemical Characterization of Different Extracts from Artemisia annua and Their Antioxidant, Enzyme Inhibitory and Anti-Inflammatory Properties.

Artemisia annua L. (Asteraceae Family) is an important plant in Asia that has been used for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation. In this study we aimed to evaluate the effects of different polarity extracts (hexane, dichloromethane, ethyl acetate, ethanol, ethanol/water (70 %) and water) from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS. In parallel, chemical composition, antiradical, and enzyme inhibition effects against α-amylase, α-glucosidase, tyrosinase, and cholinesterases were evaluated. The water extract contained the highest content of the total phenolic with 34.59 mg gallic acid equivalent (GAE)/g extract, while the hexane had the highest content of the total flavonoid (20.06 mg rutin equivalent (RE)/g extract). In antioxidant assays, the polar extracts (ethanol, ethanol/water and water) exhibited stronger radical scavenging and reducing power abilities when compared to non-polar extracts. The hexane extract showed the best AChE, tyrosinase and glucosidase inhibitory effects. All extracts revealed effective anti-inflammatory agents, as demonstrated by the blunting effects on COX-2 and TNFα gene expression. These effects seemed to be not related to the only phenolic content. However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.

The predictive factors that total laser energy consumed during retrograde intrarenal surgery (RIRS): stone area and density.

Holmium: YAG (Ho: YAG) laser lithotripsy with flexible ureterorenoscopy can be used with high stone-free and low complication rates for renal stones. This study aimed to determine the factors affecting the total laser energy in cases with provided stone-free status after a single session of retrograde intrarenal surgery (RIRS). Data of 222 patients who underwent RIRS between October 2017 and March 2020 were evaluated retrospectively. After exclusion criteria, the study was carried out with 184 stone-free cases. All cases were performed without using a ureteral access sheath (UAS), and dusting was preferred as the lithotripsy method. The effects of age, gender, body mass index (BMI), previous RIRS history, previous shock wave lithotripsy (SWL) history, stone localization, number of stones, stone surface area, and stone density on total laser energy were analyzed. There was no significant correlation between total laser energy with gender, BMI, previous RIRS history, previous SWL history, stone localization, and the number of stones (p:0.347, p:0.482, p:0.119, p:0.167, p:0.907, p:0.933 respectively). There was a significant correlation between age and total laser energy (p = 0.032), but it was not observed when the effect of the stone surface area was removed (p = 0.354). There were significant correlations between total laser energy and stone surface area, stone density, and total laser time (p<0.001, p<0.001, and p <0.001, respectively). Stone area and stone density affect the total energy consumed during laser lithotripsy. Urologists should consider the stone area, stone density, and the power of the laser device to determine which surgical technic to prefer.

Two new eudesmane-type sesquiterpene derivatives from Lecokia cretica (Lam.) DC.

Two new sesquiterpene glucosides, 1α,6ß,9ß-trihydroxy-eudesm-4(15)-en-1,6-O-ß-diglucopyranoside (1) and 1α,6ß,9ß-trihydroxy-eudesm-3-en-1,6-O-ß-diglucopyranoside (2) were obtained along with the 1α,6ß,9ß-trihydroxy-5,10-bis-epi-eudesm-3-en-6-O-ß-D-glucopyranoside (3), chlorogenic acid (4), luteolin 7-O-rutinoside (5) and luteolin 7-O- glucoside (6) from the whole plant parts of Lecokia cretica. Their structures were determined on the basis of 1 D, 2 D NMR and HRMS analyses. The in vitro cytotoxic activity of compounds 1-3 against human lung cancer cells (A549) and normal human lung cells (BEAS-2B) was determined using the MTT colorimetric assay. All the tested eudesmane derivatives were found to be inactive.

Detailed Chemical Characterization and Biological Propensities of Malabaila lasiocarpa Extracts: An Endemic Plant to Turkey.

This study focused on the biological evaluation and chemical characterization of Malabaila lasiocarpa Boiss. (M. lasiocarpa) (Family: Apiaceae). The phytochemical profile, antioxidant, enzyme inhibitory of the methanolic, aqueous, dichloromethane, hexane extracts were investigated. Based on UHPLC-HRMS analyses, a total of 101 peaks were annotated or identified for the first time in M. lasiocarpa extracts. They include hydroxybenzoic, hydroxycinnamic, acylquinic acids and their glycosides, C- and O-glycosyl and O-diglycosyl flavonoids. In addition, 10 simple mono- and disubstituted coumarins together with 10 furanocoumarins were tentatively annotated. The methanolic extract possessing the highest phenolic (24.36±0.60 mg gallic acid equivalent/g extract) and flavonoid (69.15±0.37 mg rutin equivalent/g extract) content also exhibited the strongest radical scavenging potential against 2,2-diphenyl-1 picrylhydrazyl (21.73±0.42 mg Trolox equivalent/g extract, respectively), and highest reducing capacity (57.81±0.97 and 28.00±0.40 mg Trolox equivalent/g extract, for cupric reducing antioxidant capacity and ferric reducing antioxidant power, respectively). The dichloromethane extract substantially depressed the tyrosinase (73.92±5.37 mg kojic acid equivalent/g extract), α-amylase (0.63±0.01 mmol acarbose equivalent/g extract) and α-glucosidase (0.69±0.02 mmol acarbose equivalent/g extract) enzymes. This study has produced critical scientific data on M. lasiocarpa which are potential contenders for the development of novel phyto-pharmaceuticals.

Chemical Profiling and Biological Evaluation of Nepeta baytopii Extracts and Essential Oil: An Endemic Plant from Turkey.

Nepeta baytopii is a poorly studied, endemic Nepeta species (Lamiaceae) of Turkey. For the first time, the biological activities (antioxidant, enzyme inhibition, and cytotoxicity properties) of the hexane, ethyl acetate, methanol, water/methanol, and water extracts and essential oil prepared from N. baytopii aerial parts were assessed. Hydro-methanol (41.25 mg gallic acid equivalent (GAE)/g) and water extracts (50.30 mg GAE/g), respectively showed the highest radical scavenging (94.40 and 129.22 mg Trolox equivalent (TE)/g, for 2,2-diphenyl-1-picrylhydrazyl radical and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid radical scavenging assays) and reducing (229.37 and 129.55 mg TE/g, for ferric-reducing antioxidant power and cupric-reducing antioxidant capacity assays) capacities in vitro. An interestingly high inhibition was observed for ethyl acetate extract against butyrylcholinesterase (10.85 mg galantamine equivalent/g). The methanol extract showed high cytotoxicity (31.7%) against HepG2 cells. Caryophyllene oxide was identified in high concentrations in the essential oil (39.3%). Luteolin and apigenin and their derivatives were identified from the methanol and water extracts. The results obtained from this study highlighted that the abundance of highly bioactive compounds from Nepeta baytopii ensures the multiple biological activities of the tested extracts, and this suggests a potential use in the pharmaceutical and nutraceutical fields, and therefore should be investigated further.

An ethnobotanical study on medicinal plants in Espiye and its surrounding (Giresun-Turkey).

ETHNOPHARMACOLOGICAL RELEVANCE: This paper provides significant ethnobotanical information on medicinal plants in Espiye in the Black Sea Region. Recording such data calls for urgency. This is the first ethnobotanical study in which statistical calculations about plants are carried out by means of FIC method in Northern (Espiye-Giresun) part of Turkey. This study aims to identify the wild plants collected for medicinal purposes by locals of Espiye which is located in the Black Sea Region of Turkey, and to identify the uses and local names of these wild plants. MATERIALS AND METHODS: A field study had been carried out for a period of approximately 2 years (2012-2014). During this period, information about medicinal use of 55 wild and 15 cultivated plants were collected. Demographic characteristics of participants, names of the local plants, their utilized parts and preparation methods were investigated and recorded. Through face-to-face interviews, we identified and recorded demographic characteristics of the respondents. We interviewed 128 persons who are over the age of 29. The plant taxa were collected within the scope of the study; and herbarium materials were prepared. In addition, the relative significance value of the taxa was determined, and informant consensus factor (FIC) was calculated for the medicinal plants included in the study. RESULTS: We have found out in the literature review of the plants included in our study that 70 plant taxa are already used for medicinal purposes while 3 plants are not available among the records in the literature. The most common families are Asteraceae, Rosaceae, Lamiaceae, Malvaceae, and Ericaceae. We include in our study and report for the first time the medicinal uses of Alchemilla crinita Buser, Stachys sylvatica L., and Thymus leucotrichus Hal. var. leucotrichus Hal. Names of local plants in Turkey vary especially due to vernaculars. The plants that the locals of Espiye use are called with the same or different local names in various parts of Anatolia. CONCLUSION: We found out that locals living in the research area use for therapeutic purpose 70 plants taxa which belong to 36 families. Turkish citizens with different ethnic backgrounds took the questionnaire. These people use these wild plants in treatment of several diseases. Comparison of the data obtained in this study with the experimental data obtained in the previous laboratory studies on the wild plants which grow in Espiye proved ethnobotanical usages to a great extent. Literature review indicated that the therapeutic plants that grow in Espiye are used in different parts of the world for the treatment of similar diseases.

Traditional uses of medicinal plants in Elazig (Turkey).

ETHNOPHARMACOLOGICAL RELEVANCE: This study has identified not only the wild plants collected for medical purposes by local people of Elazig Province in the Eastern Anatolia Region, but also the uses and local names of these plants. These resources are usually regarded as part of a culture׳s traditional knowledge. Therefore, the aim of this study is to collect information from local population concerning the use of medicinal plants of the Elazig region; identify the most important medicinal plants used; determine the relative importance of the species surveyed and calculate the informant consensus factor (FIC) in relation to medicinal plant use. MATERIALS AND METHODS: A field study had been carried out for a period of approximately 2 years (2012-2013). A questionnaire was administered to the local people, through face-to-face interviews (Appendix A). Demographic characteristics of participants, names of the local plants, their utilized parts and preparation methods were investigated and recorded. The plant species were collected within the scope of the study; herbarium materials were prepared; and the specimens were entitled. The collected data were used to calculate the FIC and the plant use values. RESULTS: 74 Plants were found to be used for medical purposes before in the literature analysis of the plants used in our study, while 6 plants were found to have no literature records. Our results showed that the highest use values were recorded for the species Urtica dioica L. (0.46) and Rosa canina L. (0.42), while the highest FIC was cited for skin diseases (0.60). CONCLUSION: Data obtained showed that in the studied area the folk use of plants is alive and still derives from daily practice. Evaluation of pharmacological activity for the promising medicinal plants is suggested.

Traditional uses of medicinal plants in Solhan (Bingöl-Turkey).

ETHNOPHARMACOLOGICAL RELEVANCE: This study has identified not only the wild plants collected for medical purposes by local people of Solhan District in the Eastern Anatolia Region, but also the uses and local names of these plants. It tried to provide a source for researchers studying in ethnobotany, pharmacology and chemistry by comparing the information obtained from traditionally used herbs with previous laboratory studies. AIM OF THE STUDY: This study aims to identify wild plants collected for medical purposes by the local people of Solhan District located in the Eastern Anatolia Region of Turkey and to determine the uses and local names of these plants. MATERIALS AND METHODS: A field study had been carried out for a period of approximately 2 years (2011-2012). During this period, 214 vascular plant specimens were collected. Demographic characteristics of participants, names of the local plants, their utilized parts and preparation methods were investigated and recorded. The plant species were collected within the scope of the study; herbarium materials were prepared; and the specimens were entitled. In addition, the relative importance value of the species was determined and informant consensus factor (FIC) was calculated for the medicinal plants included in the study. Our research area also includes people with Kurdish and Zaza ethnic origins. RESULTS: 82 plants were found to be used for medical purposes before in the literature analysis of the plants used in our study, while 9 plants were found to have no literature records. The most common families are Asteraceae (12 plants), Rosaceae (10 plants), and Lamiaceae (9 plants). The medicinal uses of Anthriscus cerefolium (L.) Hoffm., Arum elongnatum Steven, Astragalus lamarckii Boiss., Chaerophyllum bulbosum L., Crataegus atrosanguinea Pojark., Hordeum bulbosum L., Pastinaca armena Fisch. & Mey., Prunus kurdica Fenzl ex Fritsch, Sium sisarum L. var. lancifolium (M. Bieb.) Thell. that we found were used in our study area and recorded for the first time. No information could be obtained regarding the names of two wild plants that are being used in Solhan. In Turkey, local plant names display differences especially due to local dialects. The plants used in Solhan are known by the same or different local names in various parts of Anatolia. CONCLUSION: In the research area, local people were found to use 82 plants from 31 families for curative purposes. The respondents of the questionnaire are Turkish citizens, with various ethnic backgrounds. Mean age of the respondents was 55 years. These plants are used in the treatment of many diseases. Comparison of the data obtained in this study with the experimental data obtained in the previous laboratory studies derived from the plants growing in Solhan proved ethnobotanical usages to a great extent. Literature review indicated that the curative plants that grow in Solhan are used in different parts of the world for the treatment of similar diseases. These plants, used for the treatment of various diseases, are abundantly found in this region. Drying of the medicinal plants enabled the local people to use them in every season of the year.

An ethnobotanical survey of medicinal plants in Edremit Gulf (Balikesir-Turkey).

ETHNOPHARMACOLOGICAL RELEVANCE: This paper provides significant ethnobotanical information on medical plants in the Western Region of Turkey. There is urgency in recording such data. This is the first ethnobotanical study in which statistical calculations about plants are done by FIC method in western part of Turkey. AIM OF STUDY: This study aimed to identify plants collected for medical purposes by the local people of Edremit Gulf, located in the Western Region of Turkey, and to document the uses and local names of these plants. MATERIALS AND METHODS: This study, conducted between 2007 and 2010, gathered information on the medicinal plant species traditionally used in Edremit Bay, Turkey and the local names of these plants. In the scope of the study, medicinals plant species and related information were collected; herbarium materials were prepared; and the specimens were entitled. Field research was conducted by collecting ethnobotanical information during structured and semi-structured interviews with native knowledgeable people in territory. In addition, the relative importance value of species was determined and informant consensus factor (FIC) was calculated for the medicinal plants included in the study. RESULTS: A total of 118 medical plants belonging to 50 families were identified in the region. Among them, 99 species are wild and 19 species are cultivated plant. The most common medicinal plant families were Lamiaceae (>18%), Asteraceae (>11%), Rosaceae (>7%); the most common preparations were infusion and decoction. It was found that Hypericum perforatum, Lavandula stoechas, Salviatomentosa, Origanum onites, Origanum vulgare, and Teucrium polium were the most commonly used species. A total of 218 medicinal uses (remedies) were recorded. The traditional medicinal plants have been mostly used for the treatment of abdominal and stomach pain (17%), cough and cold (12%), diabetes (6%), kidney ailments (5%), wounds (4%). CONCLUSION: The use of traditional medicine was still widespread among the people interviewed during this study. Due to the increasing health service facilities in the area, herbal medicine, seemed to be more related to health care and disease prevention than cure.