ABSTRACT
PURPOSE: To assess the DNA damage induced by MBRT and BB radiations on glioma cells. METHODS: The analysis of fluorescent intensity emitted per nucleus was plotted versus DNA content 2 and 17 h after irradiations. At around cell-doubling time (17 h) after exposures, the remaining DNA radiation damage could be correlated with cellular death. RESULTS: A higher γH2AX IF intensity per cell could be detected 2 and 17 h after MBRT when compared with BB. 17 h after MBRT, misrepaired damaged cells remained arrested in both G1 and G2 phases. CONCLUSIONS: A pronounced G2 phase arrest was detected at 17 h after MBRT and BB. However, only after MBRT, a dose-dependent increasing number of damaged cells appeared arrested also in the G1 phase, and a higher amount of cells more prone to undergo apoptosis were detected. The threshold dose required to enhance the effectiveness of both synchrotron radiation techniques was 12 Gy.
Subject(s)
DNA Breaks, Double-Stranded/radiation effects , Glioma/genetics , Radiotherapy/methods , Animals , Cell Line, Tumor , Rats , SynchrotronsABSTRACT
During the last 30 years many groups have carried out experiments and trials to develop new imaging and radiotherapy techniques in oncology, based on the use of synchrotron X-rays. There are several synchrotron biomedical stations around the world, which offer an excellent platform to improve either the imaging diagnosis or radiotherapy treatment for different tumour types. In the coming months the first radiotherapy clinical trials will be seen at the Biomedical Beamline at the ESRF synchrotron in Grenoble (France). In this article we highlight the results of some of the techniques and strategies that have been developed at different biomedical synchrotron stations.