ABSTRACT
The efficiency of direct electron flow from electron donors to electron acceptors in redox reactions is significantly influenced by the spatial separation of these components. Geobatteries, a class of redox-active substances naturally present in soil-water systems, act as electron reservoirs, reversibly donating, storing, and accepting electrons. This capability allows the temporal and spatial decoupling of redox half-reactions, providing a flexible electron transfer mechanism. In this review, we systematically examine the critical role of geobatteries in influencing electron transfer and utilization in environmental biogeochemical processes. Typical redox-active centers within geobatteries, such as quinone-like moieties, nitrogen- and sulfur-containing groups, and variable-valent metals, possess the potential to repeatedly charge and discharge. Various characterization techniques, ranging from qualitative methods like elemental analysis, imaging, and spectroscopy, to quantitative techniques such as chemical, spectroscopic, and electrochemical methods, have been developed to evaluate this reversible electron transfer capacity. Additionally, current research on the ecological and environmental significance of geobatteries extends beyond natural soil-water systems (e.g., soil carbon cycle) to engineered systems such as water treatment (e.g., nitrogen removal) and waste management (e.g., anaerobic digestion). Despite these advancements, challenges such as the complexity of environmental systems, difficulties in accurately quantifying electron exchange capacity, and scaling-up issues must be addressed to fully unlock their potential. This review underscores both the promise and challenges associated with geobatteries in responding to environmental issues, such as climate change and pollutant transformation.
ABSTRACT
Phosphorus (P) losses from tile-drained agricultural fields may degrade surface water quality by accelerating eutrophication. Among the different edge-of-field technologies, compact filter systems using different filter materials have been identified as potentially effective solutions for removing P from drainage water before discharge downstream. This study investigated the long-term (>696 days) P removal efficiency of 5 different filter materials in a column setup, using artificial drainage water (pH 6). Filter materials included two iron-based granulates (calcinated diatomaceous earth (CDE), ferric hydroxide granules (CFH)), and three calcium-based granulates (seashells, limestone, calcinated silicate/calcium oxide (Filtralite-P)). Experiments were performed under variable flow rates (0.037 and 1.52 L h-1; hydraulic retention time of 26-43 min and 18-30 h) and inlet P concentrations (0.14 and 0.7 mg L-1). An overall analysis revealed that the Fe-based materials achieved higher P retention than Ca-based materials. In particular, CFH was capable of retaining 99 and 98 % of the high and low inlet P concentrations, respectively. Conversely, limestone retained only 25 % of the high P load. CDE performed moderately well, independently of the inlet P concentration. Filtralite-P and Seashells performed well at high inlet P concentration but relatively poorly at low P concentration. The sensitivity of filter material P removal efficiency to variations in P loading was generally lowest for CFH and highest for limestone.
Subject(s)
Bays , Phosphorus , Iron , Calcium Carbonate , SilicatesABSTRACT
Phosphorus losses from agriculture have long generated concern due to the ecological impact on surface waters. Here tile-drained agricultural catchments are a critical source for concentrating and transporting phosphorus bioavailable forms or dissolved reactive phosphorus (DRP). Hence, edge-of-field technologies have been introduced to reduce DRP loads. Filter systems have received special attention due to their targeted approach using a permeable filter material (FM) rich in DRP sorbents. This review explores the performance and applicability of FMs in the aforementioned context because of the growing number of studies. An overall analysis revealed that sorption is preferable to precipitation for DRP retention at the edge-of-field, and that FM pH and particle size affect sorption properties and subsequently DRP retention and lifetime. Thus, FMs with predominant amounts of iron and/or aluminium can be recommended. Such materials generally have an appreciable availability of DRP binding sites, strong bonds with DRP and short reaction times, as well as low desorption, which lead to good operation. On the other hand, FMs with predominant amounts of calcium and/or magnesium are restricted to catchments with favourable conditions unless they have optimal reactivity for DRP. The review also found that hydraulic retention time plays a key role in the performance and applicability of FMs, especially in those dependent on precipitation reactions. Therefore, it is crucial that FMs are designed, constructed and managed according to the catchment conditions-including normally varying flow rates and DRP concentrations-in order to ensure successful operation. This reflects in long-term, high and steady net DRP retention along with low costs, thus improving the FM cost-effectiveness, besides discharging non-harmful effluents to aquatic ecosystems.
Subject(s)
Phosphorus , Water Movements , Ecosystem , Agriculture , Particle SizeABSTRACT
Only 20% of children worldwide reach the suggested physical activity (PA) levels, and the COVID-19 restrictions seemed to have worsened this situation. In addition, physical fitness (PF) is a crucial marker of health and combined with PA could predict future health status. The aim of this study was to compare reported PA and PF levels in a sample of Italian adolescents. We administered the International PA Questionnaire and International Fitness Enjoyment Scale to 208 adolescents aged 16.0 ± 1.5 (N = 166 females, 16 ± 2.0 years) recruited from a high school in the province of Milan (Italy). The majority of the subjects were "Minimally active" but reported adequate PF levels. In particular, subjects who reported a "Very good" PF perception, had a lower PA level. The misperception of reported PA and PF from our sample could reduce the future PA level in adolescents and lead to a negative spiral of disengagement in PA. These findings should lead to more attention on and improvements to PA promotion in the adolescent population after the restrictions caused by the COVID-19 pandemic.
ABSTRACT
BACKGROUND: Squatting is a core exercise for many purposes. However, there is still controversy surrounding the practice of targeting specific muscle groups when performing the back squat with different stance widths or foot positions. Therefore, this study aimed to assess lower limb muscle activation during different form of back squat when adopting three different foot angles. METHODS: Eight male active participants (age: 24.0±0.8 years, height: 1.80±0.63 m and mass: 85.8±8.7kg) performed maximal isometric squat, back squat with an overalod of 80% of 1 repetition maximum, and countermovement jump (CMJ) when adopting three foot rotation angles: parallel (0°), +10° outward (external rotation), and +20° outward (external rotation). We calculated the root mean square of the electromyographic signals recorded from eight participant's dominant leg muscles. RESULTS: During the descending phase of the back squat, the 20° external foot rotation elicited greater activation of the biceps femoris (+35%; P=0.027) and gastrocnemius medialis (+70%; P=0.040) compared to parallel foot. There were no significant differences among the other muscles and exercise conditions. CONCLUSIONS: The +20° foot position increased BF and GasM muscle activity only during the downward phase of the back squat. Strength coaches should consider the present findings when selecting specific resistance exercises aiming to improve athletes' strength and physical fitness.
Subject(s)
Movement , Posture , Adult , Electromyography , Foot , Humans , Leg , Male , Muscle, Skeletal/physiology , Young AdultABSTRACT
Intensive agriculture requires increasing application of fertilizers in order to sustain food production. Improper use of these substances in combination with increasing seawater intrusion results in long-term and nonpoint soil and groundwater contamination. In this work, a 3-D groundwater and solute transport numerical model was created to simulate the effect of excessive fertilizers application along the Bahr El Baqar drain system, in the eastern Nile Delta, Egypt. The geotechnical properties of the soils, hydrologic parameters, and unconfined compressive strength were determined at different sites and used as input parameters for the model. Model results showed that silty clay soils are able to contain the contaminations and preserve the groundwater quality. Nevertheless, sandy soils primarily located at the beginning of the Bahr El Baqar drain allow leakage of fertilizers to the groundwater. Thus, fertilizer application should be properly managed in the top sandy layers to protect the groundwater and soil, as increasing aquifer by excess irrigation water increased the groundwater contamination in confined layers due to the high value of cumulative salt for the current situation while the unconfined zone decreased groundwater and soil contamination. A mass transport 3-D multi-species (MT3D) model was set to identify the optimal measure to tackle soil and groundwater contamination along the Bahr El-Baqar drain system. A potential increase of the abstraction rates in the study area has a positive impact in reducing the transfer of fertilizer contamination to groundwater while it has a negative impact for soil contamination. The scenario analysis further indicated that the installation of a drainage network decreases the groundwater and soil contamination. Both solutions are potentially effective for protection against nonpoint contamination along the Bahr El Baqar drain system. However, a more sustainable management approach of fertilizer application is needed to adequately protect the receptors located further downstream in the Nile Delta.
Subject(s)
Fertilizers , Groundwater , Agriculture , Egypt , Fertilizers/analysis , SoilABSTRACT
PURPOSE: Badminton is characterized by bouts of high intensity interspersed by short recovery periods. Aerobic assessment via indirect calorimetry is impractical on court because of the encumbrance of portable metabolic devices. When the relationship between heart rate (HR) and pulmonary oxygen uptake [Formula: see text] [Formula: see text] is linear, HR monitoring can provide an indirect estimation of metabolic demands on court. However, owing to the intermittent nature of badminton, the [Formula: see text] relationship will differ from that obtained in the laboratory, making its use on court questionable. The aims of this study were to (i) assess cardiorespiratory and metabolic responses during on-court badminton rally simulations at different intensities and (ii) compare [Formula: see text] relationships obtained from laboratory and on-court measurements. METHODS: The study sample was seven professional badminton players (age 16.9 ± 2.1 years; body mass 62.8 ± 9.2 kg; stature 1.71 ± 0.09 m). [Formula: see text] HR, and other respiratory and metabolic parameters were assessed in the laboratory with an incremental intermittent Astrand-type test (IIAT) and on court during rally simulations at three different intensities. RESULTS: Cardiorespiratory parameters measured during the rallies reached 95% of maximal IIAT values. The [Formula: see text] slope and intercept differed in the on-court and the IIAT conditions (P = 0.012 and P = 0.008, respectively). CONCLUSIONS: The difference in [Formula: see text] regression lines between the IIAT and the on-court condition indicates that HR monitoring may not provide accurate data on the aerobic demands of specific on-court badminton tasks. HR monitoring should be preceded by an indirect calorimetry test on court to assess aerobic demands more precisely.
Subject(s)
Exercise/physiology , Heart Rate/physiology , Lung/physiology , Oxygen Consumption/physiology , Racquet Sports/physiology , Adolescent , Exercise Test , Female , Humans , Male , Young AdultABSTRACT
Purpose: The aim of this study was to compare the effects of continuous moderate-intensity and discontinuous high-intensity training on running performance in master runners. Methods: Thirty-four male master runners (47.2 ± 7.4 years) were assigned to three different groups: continuous moderate-intensity training (CMIT), discontinuous high-intensity training (DHIT), and control group (CON). CMIT and DHIT performed 8-week of supervised training (3 session·wk-1; ~35 km·wk-1) while CON maintained their normal training habits (3-4 session·wk-1; ~50 km·wk-1). Peak oxygen consumption ([Formula: see text]O2peak) and peak running speed (vpeak) during incremental treadmill exercise, gas exchange threshold (GET), speed at GET, energy cost of running (Cr), and 5-km performance were evaluated before and after training. Results: Following the training period, both CMIT and DHIT significantly reduced Cr (-4.4 and -4.9%, respectively, P < 0.05), increased speed at GET (3.4 and 5.7%, P < 0.05) and improved 5-km time-trial performance (3.1 and 2.2%, P < 0.05) whereas no differences were found for [Formula: see text]O2peak and GET (as %[Formula: see text]O2peak). After training, vpeak improved only for DHIT (6%, P < 0.05). No differences were found in any variable for CON. Conclusions: This study indicates that both CMIT and DHIT may positively affect running performance in middle-aged master runners. This improvement was achieved despite a significant reduction of the amount of weekly training volume.
ABSTRACT
Biogeochemical processes in subsurface flow constructed wetlands are influenced by flow direction, degree of saturation and influent loading position. This study presents a simulation tool, which aims to predict the performance of the unit and improve the design. The model was developed using the HYDRUS program, calibrated and verified on previously measured bromide (Br-) pulse tracer tests. Three different hydraulic designs (Horizontal (HF), Vertical upward (VF-up), Vertical downward (VF-down) and two different flow rates: Low (L), and High (H)) were investigated. The model simulated well the Br- transport behaviour and the results underline the importance of the hydraulic design. Calibrated model parameters (longitudinal dispersivity, immobile liquid phase, mass transfer coefficient) showed a common trend for all the designs, for increasing flow rates within the investigated range. The VF-down performed best, i.e. had the highest hydraulic retention time.
Subject(s)
Agriculture , Waste Disposal, Fluid/methods , Water Movements , Water Pollutants, Chemical/chemistry , Wetlands , Water/chemistryABSTRACT
It has been reported that nitrate supplementation can improve exercise performance. Most of the studies have used either beetroot juice or sodium nitrate as a supplement; there is lack of data on the potential ergogenic benefits of an increased dietary nitrate intake from a diet based on fruits and vegetables. Our aim was to assess whether a high-nitrate diet increases nitric oxide bioavailability and to evaluate the effects of this nutritional intervention on exercise performance. Seven healthy male subjects participated in a randomized cross-over study. They were tested before and after 6 days of a high (HND) or control (CD) nitrate diet (~8.2 mmolâday(-1) or ~2.9 mmolâday(-1), respectively). Plasma nitrate and nitrite concentrations were significantly higher in HND (127 ± 64 µM and 350 ± 120 nM, respectively) compared to CD (23 ± 10 µM and 240 ± 100 nM, respectively). In HND (vs. CD) were observed: (a) a significant reduction of oxygen consumption during moderate-intensity constant work-rate cycling exercise (1.178 ± 0.141 vs. 1.269 ± 0.136 L·min(-1)); (b) a significantly higher total muscle work during fatiguing, intermittent sub-maximal isometric knee extension (357.3 ± 176.1 vs. 253.6 ± 149.0 Nm·s·kg(-1)); (c) an improved performance in Repeated Sprint Ability test. These findings suggest that a high-nitrate diet could be a feasible and effective strategy to improve exercise performance.
Subject(s)
Diet, Healthy , Exercise Tolerance , Fruit , Muscle, Skeletal/metabolism , Nitrates/administration & dosage , Nitric Oxide/metabolism , Vegetables , Adult , Bicycling , Cross-Over Studies , Exercise Test , Humans , Isometric Contraction , Italy , Male , Muscle Fatigue , Muscle Strength , Nitrates/blood , Nitrites/blood , Oxygen Consumption , Time Factors , Young AdultABSTRACT
Acute exercise induces an increase in Reactive Oxygen Species (ROS) production dependent on exercise intensity with highest ROS amount generated by strenuous exercise. However, chronic repetition of exercise, that is, exercise training, may reduce exercise-induced oxidative stress. Aim of this study was to evaluate the effects of 6-weeks high-intensity discontinuous training (HIDT), characterized by repeated variations of intensity and changes of redox potential, on ROS production and antioxidant capacity in sixteen master swimmers. Time course changes of ROS generation were assessed by Electron Paramagnetic Resonance in capillary blood by a microinvasive approach. An incremental arm-ergometer exercise (IE) until exhaustion was carried out at both before (PRE) and after (POST) training (Trg) period. A significant (P < 0.01) increase of ROS production from REST to the END of IE in PRE Trg (2.82 ± 0.66 versus 3.28 ± 0.66 µmol·min(-1)) was observed. HIDT increased peak oxygen consumption (36.1 ± 4.3 versus 40.6 ± 5.7 mL·kg(-1)·min(-1) PRE and POST Trg, resp.) and the antioxidant capacity (+13%) while it significantly decreased the ROS production both at REST (-20%) and after IE (-25%). The observed link between ROS production, adaptive antioxidant defense mechanisms, and peak oxygen consumption provides new insight into the correlation between ROS response pathways and muscle metabolic function.
Subject(s)
Electron Spin Resonance Spectroscopy , Reactive Oxygen Species/analysis , Swimming , Adult , Antioxidants/metabolism , Athletes , Exercise Test , Humans , MaleABSTRACT
PURPOSE: Recently, some studies have suggested that overall training intensity may be more important than training volume for improving swimming performance. However, those studies focused on very young subjects, and/or the difference between high-volume and high-intensity training was blurred. The aim of this study was to investigate in masters swimmers the effects of manipulation of training volume and intensity on performance and physiological variables. METHODS: A group of 10 male masters swimmers (age 32.3 ± 5.1 y) performed 2 different 6-wk training periods followed by 1 wk of tapering. The first period was characterized by high training volume performed at low intensity (HvLi), whereas the second period was characterized by low training volume performed at high intensity (LvHi). Peak oxygen consumption (VO2peak) during incremental arm exercise, individual anaerobic threshold (IAT), and 100-m, 400-m, and 2000-m-freestyle time were evaluated before and at the end of both training periods. RESULTS: HvLi training significant increased VO2peak (11.9% ± 4.9% [mean change ± 90%CL], P = .002) and performance in the 400-m (-2.8% ± 1.8%, P = .002) and 2000-m (-3.4% ± 2.9%, P = .025), with a likely change in IAT (4.9% ± 4.7%, P > .05). After LvHi training, speed at IAT (12.4% ± 5.3%, P = .004) and 100-m performance (-1.2% ± 0.8%, P = .001) also improved, without any significant changes in VO2peak, 2000-m, and 400-m. CONCLUSIONS: These findings indicate that in masters swimmers an increase of training volume may lead to an improvement of VO2peak and middle- to long-distance performance. However, a subsequent period of LvHi training maintains previous adjustments and positively affects anaerobic threshold and short-distance performance.
Subject(s)
Athletic Performance/physiology , Physical Education and Training/methods , Swimming/physiology , Adult , Anaerobic Threshold , Humans , Male , Muscle, Skeletal/physiology , Oxygen Consumption , Young AdultABSTRACT
PURPOSE: Dietary nitrate supplementation has been shown to reduce O2 cost of submaximal exercise, improve exercise tolerance, and enhance performance in moderately trained individuals. In contrast, data have been provided that elite athletes do not benefit from nitrate supplementation. The aim of this study was to evaluate the effects of short-term nitrate supplementation on endurance performance in subjects with different levels of aerobic fitness. METHODS: Twenty-one subjects (mean age, 22.7 ± 1.8 yr) with different aerobic fitness level (VËO2peak value ranging from 28.2 to 81.7 mL·kg·min) participated in a crossover double-blind placebo-controlled study. Subjects were tested after 6 d of supplementation with either 0.5 l per day of nitrate (5.5 mmol)-containing water (NITR) or nitrate-free water (PLA). Participants performed an incremental running test until exhaustion and four repetitions of 6-min submaximal (approximately 80% of gas exchange threshold) constant load exercise on a motorized treadmill. Moreover, subjects performed a 3-km running time trial on the field. RESULTS: After NITR, a negative correlation between reduction of O2 cost of submaximal exercise and individual aerobic fitness level was observed (r = 0.80; P < 0.0001). A significant inverse correlation was also found between aerobic fitness level and improvement in performance for 3-km time trial after NITR (r = 0.76; P < 0.0001). Additionally, subjects responded differently to dietary nitrate supplementation according to aerobic fitness level with higher-fit subjects showing a lower increase in plasma [NO3] (r = 0.86; P < 0.0001) and [NO2] (r = 0.75; P < 0.0001). CONCLUSIONS: The results of the present study suggest that the individual aerobic fitness level affects the ergogenic benefits induced by dietary nitrate supplementation. The optimal nitrate loading regimen required to elevate plasma [NO2] and to enhance performance in elite athletes is different from that of low-fit subjects and requires further studies.
Subject(s)
Athletic Performance , Dietary Supplements , Nitrates/pharmacology , Physical Endurance/drug effects , Physical Fitness/physiology , Athletes , Cross-Over Studies , Double-Blind Method , Humans , Nitrates/administration & dosage , Oxygen Consumption , Young AdultABSTRACT
Traditionally, Live High-Train High (LHTH) interventions were adopted when athletes trained and lived at altitude to try maximising the benefits offered by hypoxic exposure and improving sea level performance. Nevertheless, scientific research has proposed that the possible benefits of hypoxia would be offset by the inability to maintain high training intensity at altitude. However, elite athletes have been rarely recruited as an experimental sample, and training intensity has almost never been monitored during altitude research. This case study is an attempt to provide a practical example of successful LHTH interventions in two Olympic gold medal athletes. Training diaries were collected and total training volumes, volumes at different intensities, and sea level performance recorded before, during and after a 3-week LHTH camp. Both athletes successfully completed the LHTH camp (2090 m) maintaining similar absolute training intensity and training volume at high-intensity (> 91% of race pace) compared to sea level. After the LHTH intervention both athletes obtained enhancements in performance and they won an Olympic gold medal. In our opinion, LHTH interventions can be used as a simple, yet effective, method to maintain absolute, and improve relative training intensity in elite endurance athletes. Key PointsElite endurance athletes, with extensive altitude training experience, can maintain similar absolute intensity during LHTH compared to sea level.LHTH may be considered as an effective method to increase relative training intensity while maintaining the same running/walking pace, with possible beneficial effects on sea level performance.Training intensity could be the key factor for successful high-level LHTH camp.
ABSTRACT
Beneficial systemic effects of regular physical exercise have been demonstrated to reduce risks of a number of age-related disorders. Antioxidant capacity adaptations are amongst these fundamental changes in response to exercise training. However, it has been claimed that acute physical exercise performed at high intensity (>60% of maximal oxygen uptake) may result in oxidative stress, due to reactive oxygen species being generated excessively by enhanced oxygen consumption. The aim of this study was to evaluate the effect of high-intensity discontinuous training (HIDT), characterized by repeated variations of intensity and changes of redox potential, on oxidative damage. Twenty long-distance masters runners (age 47.8 ± 7.8 yr) on the basis of the individual values of gas exchange threshold were assigned to a different 8-weeks training program: continuous moderate-intensity training (MOD, n = 10) or HIDT (n = 10). In both groups before (PRE) and after (POST) training we examined the following oxidative damage markers: thiobarbituric acid reactive substances (TBARS) as marker of lipid peroxidation; protein carbonyls (PC) as marker of protein oxidation; 8-hydroxy-2-deoxy-guanosine (8-OH-dG) as a biomarker of DNA base modifications; and total antioxidant capacity (TAC) as indicator of the overall antioxidant system. Training induced a significant (p<0.05) decrease in resting plasma TBARS concentration in both MOD (7.53 ± 0.30 and 6.46 ± 0.27 µM, PRE and POST respectively) and HIDT (7.21 ± 0.32 and 5.85 ± 0.46 µM, PRE and POST respectively). Resting urinary 8-OH-dG levels were significantly decreased in both MOD (5.50 ± 0.66 and 4.16 ± 0.40 ng mg(-1)creatinine, PRE and POST respectively) and HIDT (4.52 ± 0.50 and 3.18 ± 0.34 ng mg(-1)creatinine, PRE and POST respectively). Training both in MOD and HIDT did not significantly modify plasma levels of PC. Resting plasma TAC was reduced in MOD while no significant changes were observed in HIDT. In conclusion, these results suggest that in masters runners high-intensity discontinuous does not cause higher level of exercise-induced oxidative stress than continuous moderate-intensity training, inducing similar beneficial effects on redox homeostasis.
Subject(s)
Exercise/physiology , Homeostasis/physiology , Lipid Peroxidation/physiology , Oxidative Stress/physiology , Reactive Oxygen Species/blood , Running/physiology , Adolescent , Adult , Biomarkers/blood , Humans , MaleABSTRACT
A recent study has demonstrated that neuromuscular electrical stimulation (NMES) determines, in vitro, a fast-to-slow shift in the metabolic profile of muscle fibers. The aim of the present study was to evaluate if, in the same subjects, these changes would translate, in vivo, into an enhanced skeletal muscle oxidative metabolism. Seven young men were tested (cycle ergometer) during incremental exercises up to voluntary exhaustion and moderate and heavy constant-load exercises (CLE). Measurements were carried out before and after an 8-wk training program by isometric bilateral NMES (quadriceps muscles), which induced an â¼25% increase in maximal isometric force. Breath-by-breath pulmonary O(2) uptake (Vo(2)) and vastus lateralis oxygenation indexes (by near-infrared spectroscopy) were determined. Skeletal muscle fractional O(2) extraction was estimated by near-infrared spectroscopy on the basis of changes in concentration of deoxygenated hemoglobin + myoglobin. Values obtained at exhaustion were considered "peak" values. The following functional evaluation variables were unaffected by NMES: peak Vo(2); gas exchange threshold; the Vo(2) vs. work rate relationship (O(2) cost of cycling); changes in concentration of deoxygenated hemoglobin + myoglobin vs. work rate relationship (related to the matching between O(2) delivery and Vo(2)); peak fractional O(2) extraction; Vo(2) kinetics (during moderate and heavy CLE) and the amplitude of its slow component (during heavy CLE). Thus NMES did not affect several variables of functional evaluation of skeletal muscle oxidative metabolism. Muscle hypertrophy induced by NMES could impair peripheral O(2) diffusion, possibly counterbalancing, in vivo, the fast-to-slow phenotypic changes that were observed in vitro, in a previous work, in the same subjects of the present study.