FUNCTIONING OF NO-CYCLE IN THE ORAL FLUID IN CHILDREN OF PRIMARY SCHOOL AGE WITH TYPE 1 DIABETES MELLITUS IN THE TREATMENT OF CHRONIC CATARRHAL GINGIVITIS.

OBJECTIVE: The aim: To determine the activity of NO-synthase and arginase in oral fluid in children with type 1 diabetes mellitus and to evaluate the efficacy of the treatment scheme we elaborated in the treatment of chronic catarrhal gingivitis. PATIENTS AND METHODS: Materials and methods: 82 children were examined, they were divided into groups by presence of gingivitis and diabetes mellitus. NO-synthase (NOS) activity was determined in oral fluid by the difference in nitrite concentration before and after incubation. The arginase activity was determined in oral fluid by the difference in the concentration of L-ornithine before and after incubation. RESULTS: Results: Use our treatment scheme in children with chronic catarrhal gingivitis and type 1 diabetes mellitus lead to a change in the polarization of oral macrophages towards the predominance of M2 polarization in 1 month. The polarization of macrophages changed to the predominance of M1 polarization activity in 1 year. CONCLUSION: Conclusions: We have elaborated a scheme for the treatment of chronic catarrhal gingivitis in children with type 1 diabetes mellitus. It normalizes the polarization of oral macrophages caused by exposure to chronic catarrhal gingivitis as a local pathogenetic factor.

[The reactions of lipid's free radical oxidation, hemocoagulant properties of oral fluid in patients with galvanic currents in the mouth].

OBJECTIVE: Introduction: Oral manifestation of galvanic currents due to the presence of two and more dissimilar metals in dental restorations is rather important in prosthodontics. The aim of our study was to assess the reactions lipid's free radical oxidation and hemocoagulant properties of oral fluid in patients with disorders in oral cavity due to galvanic currents. PATIENTS AND METHODS: Materials and methods: The study had got some groups. The first one consisted of 7 healthy patients without dental prosthesis. The other one included 22 patients with dental prosthesis and they were suffered from short circuiting due dissimilar metallic restorations in oral cavity. This group we were divided into two groups: the second one - for 7 patients were produced metal ceramic fixed dental prosthesis, the third one - for 15 patients were produced whole casted fixed dental prosthesis and pressing brazed fixed dental prosthesis. To assess the process of lipid's free radical oxidation of oral fluid we studied kinetic concentration of malone dialdehyde, activity level of superoxide dismutase and catalase. Also we found out hemocoagulant activity data of oral fluid: thrombin time, prothrombin time, fibrinolytic activity, recalcification time. RESULTS: Results: In our study we observed increasing of lipid's free radical oxidation in patients of the second and third groups, which led to increasing malone dialdehyde data in third group compared with second group. In patients of second and third groups was recorded the increasing of the CAT activity in the oral fluid. Also there is the in fact decreasing in hemocoagulant properties of oral fluid, such as reducing in recalcification time, increasing in prothrombin time in the second and especially in the third groups of patients. It was marked the significant increasing of fibrinolytic activity of oral fluid in the second and third group of patients. CONCLUSION: Conclusions: The oral manifestation of electrochemical interactions due to presence of dissimilar metallic restorations led to shift in oral cavity's homeostasis. This statement we can verify with results of our study. In patients with oral manifestation of galvanic currents were observed the increasing in activity of the reactions of lipid's free radical oxidation in oral fluid, changes in antioxidative enzymes and in local homeostasis of oral fluid.