Natural Polysaccharide Delivery Platforms with Multiscale Structure Used for Cancer Chemoimmunotherapy.

Chemoimmunotherapy is an effective cancer treatment method. Drugs are always combined and used in treating cancer. However, the characteristic of drugs varies, making it challenging to control their release kinetics utilizing delivery devices with a single microstructure. In this study, we attempted to uniformly size drugs of varying molecular weights and confine them in a compartment where immune cells may be recruited and moved freely. Dextran microgels were created as modular drug libraries to address the cryogel burst release of small molecule drugs. Then, modular drug libraries and granulocyte-macrophage colony-stimulating factor (GM-CSF) were integrated into cryogels for a combined treatment. Herein, alginate was zwitterion modified to avoid the immune reaction generated by the material. Because of its macroporous structure, the cryogel could be injected into the body, eliminating invasive surgical procedures. Results demonstrated that multiscale delivery platforms could improve the synergistic effect of various medications on tumor treatment.

"Self-Defensive" Antifouling Zwitterionic Hydrogel Coatings on Polymeric Substrates.

In biomedicine fields, biofouling can easily occur on devices such as sensors and catheters, causing some iatrogenic infections, which menace the lives and health of patients greatly. Therefore, it is of great significance to solve the problems of bacterial infection on the surfaces of medical devices. In this paper, "self-defensive" and antifouling zwitterionic hydrogel coatings were prepared by network interpenetration of the hydrogel and the polymeric substrates. The zwitterionic polysulfobetaine methacrylate (PSBMA) hydrogel coatings resisted most of the bacteria to adhere on the substrates. When a few bacteria were lucky to escape the antifouling defense and adhered to the coatings, gentamicin sulfate (GS) would be released under the trigger of a weakly acidic environment caused by bacterial metabolism to kill these bacteria. Simultaneously, the coatings of the bacteria-adhering sites would be degraded by hyaluronidase secreted by these bacteria and peeled off to remove the bacteria and renew the antifouling surfaces. The antifouling properties and mechanism of the self-defensive behavior of the hydrogel coatings on polymeric substrates were investigated. Furthermore, the in vitro and in vivo antibacterial performances, as well as the biocompatibility of the coatings, were demonstrated. The results suggested that the self-defensive antifouling zwitterionic hydrogel coatings hold great potential to be used on the surfaces of polymeric medical devices.

Highly antifouling, biocompatible and tough double network hydrogel based on carboxybetaine-type zwitterionic polymer and alginate.

Because of resistance to bio-macromolecular adhesion, antifouling hydrogels have attracted great attention in biomedical field. But traditional antifouling hydrogels made by hydrophilic polymers are always poor of mechanical properties. Herein, a new hybrid ionic-covalent cross-linked double network (DN) hydrogel was prepared by a simple one-pot method based on sodium alginate and the zwitterionic material carboxybetaine acrylamide (CBAA). The DN hydrogel has good mechanical properties, including high elastic modulus (0.28 MPa), high tensile strength (0.69 MPa), as well as good self-recovery capability. More importantly, the DN hydrogel is highly resistance to the adsorption of non-specific protein, cells, bacteria and algae, exhibiting an outstanding antifouling property. The in vitro and in vivo experiments prove that the DN hydrogel is highly biocompatible. This study provides a new strategy for the preparation of antifouling DN hydrogels with good mechanical properties for different needs, such as tissue scaffolds, wound dressings, implantable devices, and other fields.