Hepatic regulator of G protein signaling 14 ameliorates NAFLD through activating cAMP-AMPK signaling by targeting Giα1/3.

OBJECTIVE: Nonalcoholic fatty liver disease (NAFLD) is an emerging public health threat as the most common chronic liver disease worldwide. However, there remains no effective medication to improve NAFLD. G protein-coupled receptors (GPCRs) are the most frequently investigated drug targets family. The Regulator of G protein signaling 14 (RGS14), as an essential negative modulator of GPCR signaling, plays important regulatory roles in liver damage and inflammatory responses. However, the role of RGS14 in NAFLD remains largely unclear. METHODS AND RESULTS: In this study, we found that RGS14 was decreased in hepatocytes in NAFLD individuals in a public database. We employed genetic engineering technique to explore the function of RGS14 in NAFLD. We demonstrated that RGS14 overexpression ameliorated lipid accumulation, inflammatory response and liver fibrosis in hepatocytes in vivo and in vitro. Whereas, hepatocyte specific Rgs14-knockout (Rgs14-HKO) exacerbated high fat high cholesterol diet (HFHC) induced NASH. Further molecular experiments demonstrated that RGS14 depended on GDI activity to attenuate HFHC-feeding NASH. More importantly, RGS14 interacted with Guanine nucleotide-binding protein (Gi) alpha 1 and 3 (Giα1/3, gene named GNAI1/3), promoting the generation of cAMP and then activating the subsequent AMPK pathways. GNAI1/3 knockdown abolished the protective role of RGS14, indicating that RGS14 binding to Giα1/3 was required for prevention against hepatic steatosis. CONCLUSIONS: RGS14 plays a protective role in the progression of NAFLD. RGS14-Giα1/3 interaction accelerated the production of cAMP and then activated cAMP-AMPK signaling. Targeting RGS14 or modulating the RGS14-Giα1/3 interaction may be a potential strategy for the treatment of NAFLD in the future.

Association between endothelin-1, nitric oxide, and Gensini score in chronic coronary syndrome.

BACKGROUND: Chronic coronary syndrome (CCS) is a major public health burden; its pathogenesis involves atherosclerosis and endothelial dysfunction. Endothelin-1 (ET-1) and nitric oxide (NO) are vasoactive substances synthesized by endothelial cells that play a crucial role in CCS development. The Gensini score (GS) is used for evaluating CCS severity based on lumen segment changes, stenosis degree, and coronary stenosis site. METHODS: This prospective study included 71 patients with CCS; we evaluated the relationships between GS and ET-1 and NO serum levels were evaluated in these patients. The GS was calculated for all patients. Serum ET-1 & NO levels among other laboratory parameters were measured. RESULTS: The high GS group had higher ET-1 and relatively NO expressions in the than the low GS group. GS was positively correlated with ET-1 and negatively correlated with NO, T4, and TSH levels. The results of the multiple linear regression analysis showed that ET-1 had the most significant effect on GS. CONCLUSIONS: We found a strong association between ET-1, NO, and CCS severity. A combination of ET-1, NO, and GS is an essential predictor of CCS disease severity.

Anchoring tungsten oxide nanorods on TiO2 nanowires coupled with carbon for efficient lithium-ion storage.

Reasonable construction of hierarchical electrode materials is verified as a promising way to improve the electrochemical performance due to the synergistic effect between unique components and constructions. Hence, a hierarchical nanostructure composed of tungsten oxide nanorods anchored on TiO2 nanowires coupled with a carbon layer (TiO2@WOx-C NWs) was synthesized as an electrode material by exploiting the self-assembly function of dopamine and carbonization. The inner one-dimensional TiO2 nanowires served as the stable substrate with WOx anchored on the surface of TiO2 NWs and the tightly coupled carbon nanosheets, which can not only facilitate electron transport but also provide more active sites for electrochemical reactions. As a result, benefitting from the synergistic effects between three functional components and the multi-dimensional hierarchical structures, the as-prepared TiO2@WOx-C NWs displayed excellent lithium storage performance with a specific capacity of 651.4 mA h g-1 after 500 cycles at 1.0 A g-1, which is superior to most Ti-based structures. The enhanced electrochemical performance is mainly attributed to the synergistic effect of the different dimensional structures, the high capacity of tungsten oxide and the surface coating of the conductive carbon material. This work provides a simple and effective approach to designing functional hierarchical structures for energy storage and conversion.

Imaging and component analysis of pumpkin stem tissue with simultaneous SF-CARS and TPEF microscopy.

A multimodal nonlinear optical imaging platform based on a single femtosecond oscillator is built for simultaneous TPEF and SF-CARS imaging. TPEF microscopy and SF-CARS microscopy is utilized for mapping the distribution of the lignin component and the polysaccharide component, respectively. Visualization of vessel structure is realized. And the relative distribution of lignin and polysaccharide of vessel structure is mapped. Two pumpkin stem tissue areas with different degrees of lignification are observed with simultaneous TPEF and SF-CARS imaging, and two types of cell walls are identified. The different distribution patterns of lignin and polysaccharide in these two types of cell walls, induced by different degrees of lignification, are analyzed in detail.

Refractive index SPR sensor with high sensitivity and wide detection range using tapered silica fiber and photopolymer coating.

This paper introduces a surface plasmon resonance (SPR) sensor using tapered silica fiber and photopolymer coating for enhanced refractive index (RI) detection. Tapering the silica fiber to a diameter of 10 µm ensures the evanescent wave leaks into a 1.8-µm thick photopolymer film, which increases the average waveguide RI and broadens the RI detection range accordingly. A 50-nm thick single-side gold film is coated on the photopolymer film, exciting SPR and causing less light transmission loss than a double-side gold film. The method avoids the complex microfabrication processes of conventional polymer optical fiber SPR sensors, while the waveguide RI can be controlled by altering the curing time of the photopolymer during fabrication. The sensor has an overall sensitivity of 3686.25 nm/RIU, enabling RI detection of 1.333 - 1.493. Moreover, the sensor has an ultrahigh sensitivity of 6422.9 nm/RIU in the RI range of 1.423 - 1.493. The temperature response is about 1.43 nm/°C at 20 - 50 °C, which has little impact on RI detection. Finally, we demonstrate that the sensor can grade the severity of hepatic steatosis by measuring the RIs of cytoplasm/triglyceride emulsions with superior sensing performance.

Wearable Fiber SPR Respiration Sensor Based on a LiBr-Doped Silk Fibroin Film.

Respiration is essential for supporting human body functions. However, a biocompatible fiber respiration sensor has rarely been discussed. In this study, we propose a wearable fiber surface plasmon resonance (SPR) respiration sensor using a LiBr-doped silk fibroin (SF) film. The SPR sensor monitors respiration by responding to airway humidity variation during inhalation and exhalation. We fabricated the SPR respiration sensor by depositing the core of a plastic-clad optical fiber with a gold film and an SF-LiBr composite film. The SF-LiBr composite film can absorb water through the interaction between water molecules and hydrogen bonds linking fibroin chains. Thus, humidity variation can change the SF-LiBr composite film's refractive index (RI), altering the phase-matching condition of the surface plasmon polaritons and shifting the SPR spectral dip. In experiments, we test the effect of the LiBr doping ratio on humidity response and confirm that the SF-22.1 wt % LiBr sensor has balanced performances. The SF-22.1 wt % LiBr sensor has a broad sensing range of 35-99% relative humidity (RH), a reasonable overall sensitivity of -6.5 nm/% RH, a fast response time of 135 ms, a quick recovery time of 150 ms, good reversibility, and good repeatability, which is capable of tracking different respiration states and patterns. Finally, we encapsulate this sensor in a conventional nasal oxygen cannula for wearable respiration monitoring, proving that the sensor is suitable for high-sensitivity, real-time, and accurate respiration monitoring.

The Effect of Caudal Ropivacaine and Morphine on Postoperative Analgesia in Total Laparoscopic Hysterectomy: A Prospective, Double-Blind, Randomized Controlled Trial.

Purpose: Multiple regional nerve blocks proved no additional benefit in total laparoscopic hysterectomy in multimodal analgesia, as postoperative pain may mainly originate from the vaginal cuff. Theoretically, caudal block can relieve pain from the vaginal cuff by a sacral spinal nerve block. We aimed to verify whether a caudal block with ropivacaine and morphine can achieve an analgesic effect without additional adverse effects after a total laparoscopic hysterectomy. Patients and Methods: Forty-eight patients undergoing total laparoscopic hysterectomy were randomly allocated to receive preoperative caudal block with 20 mL of mixture including 0.25% ropivacaine and 2 mg morphine (caudal block group) or sham block (sham group). The primary outcome was the postoperative 24 h cumulative sufentanil consumption. Results: Median (IQR) sufentanil consumption in the first 24 postoperative hours of the caudal block group and the sham group was 0.00 (0.00 to 0.05) µg/kg vs 0.13 (0.04 to 0.21) µg/kg, respectively, p < 0.001. The majority of patients felt that visceral pain was more intense than incisional pain at 1, 6, 12, and 24 h post-surgery in the sham group (95.8% at 1 h, 95.8% at 6 h, 95.8% at 12 h, and 75% at 24 h post-surgery). Compared to the sham group, the caudal block reduced visceral pain scores at rest and during movement at 1 h (p < 0.001), 6 h (p < 0.001), 12 h (p < 0.001), and 24 h (p < 0.001) post-surgery. Intraoperative remifentanil consumption was significantly lower in the caudal block group than in the sham group (p = 0.004). There were no significant differences in other secondary outcomes between the two groups. Conclusion: A caudal block with ropivacaine and morphine could provide a satisfactory analgesic effect for 24 h postoperatively without additional adverse effects after total laparoscopic hysterectomy.

Fiber laser strain sensor based on an optical phase-locked loop.

In this Letter, we present a high-strain resolution fiber laser-based sensor (FLS) by a novel optical phase-locked loop (OPLL) interrogation technique based on a root mean square detector (RMSD). The sensor consists of a distributed feedback (DFB) fiber laser as a master laser for strain sensing and a fiber Fabry-Perot interferometer (FFPI) as a reference. The laser carrier locks to the reference by the PDH technique, and the single sideband laser working as a slave laser locks to the DFB sensing element using the OPLL technique, respectively. A strain resolution of 8.19 pε/√Hz at 1 Hz and 35.5 pε in 10 s is achieved in the demonstrational experiments. Significantly, the noise behaves a 1∕f distribution below 0.2 Hz due to the very low pump power for the DFB sensor and an active thermostat testing environment. The proposed OPLL interrogation brings new thinking for the demodulation of FLS. This strain sensor based on FLS has a great performance in strain measurement and can be a powerful tool for geophysical research.

Identification of Potential Gene Targets for Suppressing Oviposition in Holotrichia parallela Using Comparative Transcriptome Analysis.

Holotrichia parallela is an important plant pest. Comparative feeding experiments showed that the egg production, oviposition duration and survival rate of H. parallela beetles were significantly higher when they fed on elm leaves than when they fed on willow or purpus privet leaves. RNA sequencing was used to determine transcriptomic changes associated with oviposition. Comparative transcriptome analysis revealed that the beetles that fed on elm and willow had a total of 171 genes with differential expression. When the beetles fed on elm and purpus privet, 3568 genes had differential expression. The vitellogenesis, ovarian serine protease, odorant-binding proteins, acyl-CoA synthetase and follicle cell proteins were commonly upregulated genes in elm-fed beetles compared with those fed on willow/purpus privet leaves. The involvement of the follicle cell protein 3C gene in the regulation of oviposition was confirmed using RNA interference. The results provide insights into the molecular mechanisms underlying oviposition in H. parallela feeding on different host plants. This study also describes a method for identifying potentially effective genes for pest control.

Anterior Quadratus Lumborum Block for Total Laparoscopic Hysterectomy: A Randomized, Double-Blind, Placebo-Controlled Trial.

OBJECTIVES: This study aimed to investigate the perioperative analgesic effects of anterior quadratus lumborum block (QLB) for total laparoscopic hysterectomy (TLH). MATERIALS AND METHODS: One hundred patients undergoing TLH were randomized to receive an anterior QLB or placebo before general anesthesia. The primary and secondary outcomes were postoperative sufentanil consumption, intraoperative remifentanil demand, time to first opioid demand, numeric rating scale (NRS) pain scores, heart rate, mean arterial pressure, and complications within 24 hours after surgery. RESULTS: No significant intergroup differences were observed in sufentanil consumption within 24 hours after surgery. Remifentanil consumption during surgery was lower in the QLB group than in the placebo group. At 1 hour after surgery, the NRS scores of abdominal visceral pain at rest and during activity were 1.26 and 1.41 points lower than those in the placebo group. In other time points, the differences in abdominal visceral pain were neither statistically significant nor clinically significant (pain difference <1) or both. No significant differences in NRS scores of shoulder pain, abdominal incisional pain, and perineal pain were observed between the 2 groups, at rest or during activity. There were no significant differences in other secondary outcome variables between the 2 groups. DISCUSSION: Preoperative bilateral anterior QLB only reduced intraoperative opioid demand and postoperative abdominal visceral pain scores at 1 hour after surgery. Thus, the clinical significance of anterior QLB in TLH may be limited.

Spinal cord stimulation and deep brain stimulation for disorders of consciousness: a systematic review and individual patient data analysis of 608 cases.

The application of spinal cord stimulation (SCS) and deep brain stimulation (DBS) for disorders of consciousness (DoC) has been increasingly reported. However, there is no sufficient evidence to determine how effective and safe SCS and DBS are for DoC owing to various methodological limitations. We conducted a systematic review to elucidate the safety and efficacy of SCS and DBS for DoC by systematically reviewing related literature by searching PubMed, EMBASE, Medline, and Cochrane Library. Twenty eligible studies with 608 patients were included in this study. Ten studies with 508 patients reported the efficacy of SCS for DoC, and the estimated overall effectiveness rate was 37%. Five studies with 343 patients reported the efficacy of SCS for VS, and the estimated effectiveness rate was 30%. Three studies with 53 patients reported the efficacy of SCS for MCS, and the estimated effectiveness rate was 63%. Five studies with 92 patients reported the efficacy of DBS for DoC, and the estimated overall effectiveness rate was 40%. Four studies with 63 patients reported the efficacy of DBS for VS, and the estimated effectiveness rate was 26%. Three studies with 19 patients reported the efficacy of DBS for MCS, and the estimated effectiveness rate was 74%. The adverse event rate of DoC was 8.1% and 18.2% after SCS and DBS, respectively. These results suggest that SCS and DBS can be considered reasonable treatments for DoC with considerable efficacy and safety.

Runners with better cardiorespiratory fitness had higher prefrontal cortex activity during both single and exercise-executive function dual tasks: an fNIRS study.

Objective: This study investigated the relationship between executive function and prefrontal cortex oxygenation during exercise in young adults with different Cardiorespiratory fitness (CRF) levels. Methods: A total of 28 amateur runners (n = 14) and sedentary college students (n = 14) were recruited. The maximum oxygen uptake estimated for the sub-maximal intensity run (4.97 miles/h) was used to indicate the different CRF levels. After 1 week, participants must complete the Stroop and 2-Back tasks in silence while performing moderate-intensity exercise. Using 19-channel functional near-infrared spectroscopic (fNIRS) to examine changes in prefrontal cortex oxyhemoglobin. Results: There was no significant difference in the correctness of the Stroop and 2-Back tasks between the two groups during exercise, but the amateur runner group showed an acceleration in reaction time. fNIRS results showed that during the exercise 2-Back task, the left dorsolateral prefrontal cortex oxyhemoglobin was higher in the amateur runner group than in the sedentary group. Conclusion: Executive function during exercise was similarly improved in participants with better fitness, suggesting that CRF provides an excellent metabolic reserve and directed allocation for cognitive tasks during exercise.

Fiber liquid-pressure sensor with high sensitivity and a wide measurement range using photopolymer.

This paper presents a novel fiber liquid-pressure sensor that uses photopolymer glue to generate Fabry-Perot (F-P) interference, resulting in high sensitivity and a wide measurement range. The sensor comprises a single-mode fiber and photopolymer glue; the latter adheres to the fiber's end face and is decomposed by a 405-nm laser to create an air channel with a diameter of 5.9 µm and a length of 50 µm. When the air channel is placed underwater, a 17.5-µm air cavity forms between the fiber core and the air-liquid boundary due to the pressure balance, creating an F-P interferometer. Based on experimental results, the sensor has an average pressure sensitivity of 5.68 nm/kPa over 0.49-2.94 kPa. The sensitivity can be maintained at this level across different pressure measurement ranges (up to about 500 kPa) by using a 980-nm laser's radiation pressure to reset the air-liquid boundary. Besides its high sensitivity and wide measurement range, the sensor's straightforward structure, durability, affordability, compactness, and simple construction make it an appealing choice for liquid pressure measurement applications in various fields.

Functional network segregation is associated with higher functional connectivity in endurance runners.

Neuroimaging studies have identified significant differences in brain structure, function, and connectivity between endurance runners and healthy controls. However, the topological organization of large-scale functional brain networks remains unexplored in endurance runners. Using resting-state functional magnetic resonance imaging data, this study examined the differences in the topological organization of functional networks between endurance runners (n = 22) and healthy controls (n = 20). Endurance runners had significantly higher clustering coefficients in the whole-brain functional network than healthy controls, but the two did not differ regarding the shortest path length or small-world index. Using network-based statistics, we identified one subnetwork in endurance runners with higher functional connectivity than healthy controls, and the mean functional connectivity of the subnetwork significantly correlated with the three aforementioned small-world parameters. In this subnetwork, the mean clustering coefficient of nodes associated with short-range connections was higher in endurance runners than in healthy controls, but the mean clustering coefficient of nodes associated with long-range connections did not differ between the two groups. In conclusion, using graph theoretical approaches, we revealed significant differences in the topological organization of the whole-brain functional network and functional connectivity between endurance runners and healthy controls. The relationship between these two features suggests that a more segregated network may arise from the optimization of the identified subnetwork in endurance runners. These findings are possibly the neural basis underlying the good performance of endurance runners in endurance running.

Life table study of Spodoptera frugiperda at different wheat stages and the effect of larval population density on wheat yield.

BACKGROUND: Spodoptera frugiperda is an invasive, widespread agricultural pest in China. However, there have been no reports assessing feeding damage on wheat caused by S. frugiperda. To clarify the fitness and potential damage of S. frugiperda to wheat, this study analyzed the population parameters of S. frugiperda fed on wheat in a laboratory and simulated the potential damage in field conditions. RESULTS: The population parameters of S. frugiperda were compared using life tables on wheat at the seedling and adult plant stages. The adult female longevity of S. frugiperda varied from 12.29 days on seedling plants to 16.60 days on adult plants. Egg production was significantly higher when fed on wheat at the seedling stage (646.34 eggs) than when fed on adult plants (495.86 eggs). On wheat at the seedling and adult plant stages, the mean generation times were 35.42 and 38.34 days, respectively, and the intrinsic rates of increase were 0.15 and 0.14, respectively. Spodoptera frugiperda completed development and increased its population in wheat at both plant growth stages. In the field, the effect of different larval densities on the 1000-kernel weight of wheat was significantly different. An action threshold of 40 larvae per m2 was estimated, and the higher population densities caused a yield loss of 17.7%. CONCLUSION: Spodoptera frugiperda can complete its life cycle on wheat at different stages. Wheat can serve as an alternative host for S. frugiperda. If S. frugiperda reaches 320 larvae per m2 density during wheat growth, it will cause yield loss exceeding 17%. © 2023 Society of Chemical Industry.

Tuning Chirality of Self-Assembled PTCDA Molecules on a Au(111) Surface by Na Coordination.

Chiral nanostructures are much desired in many applications, such as chiral sensing, chiroptics, chiral electronics, and asymmetric catalysis. In building chiral nanostructures, the on-surface metal-organic self-assembly is naturally suitable in obtaining atomically precise structures, but that is under the premise that there are enantioselective assembly strategies to create large-scale homochiral networks. Here, we report an approach to build chiral metal-organic networks using 3,4,9,10-perylene tetracarboxylic dianhydride (PTCDA) molecules and low-cost sodium chloride (NaCl) in a controllable manner on Au(111). The chirality induction and transfer processes during network evolution with increased Na ion ratios were captured by scanning tunneling microscopy (STM), X-ray photoelectron spectroscopy (XPS), and density functional theory (DFT) methodologies. Our findings show that Na ion incorporation into achiral PTCDA molecules partially breaks intermolecular hydrogen bonds and coordinates with carboxyl oxygen atoms, which initiates a collective sliding motion of PTCDA molecules along specific directions. Consequently, "molecular columns" linked by hydrogen bonds were formed in the rearranged Na-PTCDA networks. Notably, the direction of Na ion incorporation determines the chiral characteristic by guiding the sliding direction of the molecular columns, and chirality can be transferred from Na0.5PTCDA to Na1PTCDA networks. Furthermore, our results indicate that the chirality transferring process is disrupted when intermolecular hydrogen bonds are entirely replaced by Na ions at a high Na dopant concentration. Our study provides fundamental insights into the mechanism of coordination-induced chirality in metal-organic self-assemblies and offers potential strategies for synthesizing large homochiral metal-organic networks.

Short-term exposure to enrofloxacin causes hepatic metabolism disorder associated with intestinal flora dysbiosis in adult marine medaka (Oryzias melastigma).

Enrofloxacin (ENR) is a widely used fluoroquinolone antibiotic that is frequently detected in the environment. Our study assessed the impact of short-term ENR exposure on the intestinal and liver health of marine medaka (Oryzias melastigma) using gut metagenomic shotgun sequencing and liver metabolomics. We found that ENR exposure resulted in imbalances of Vibrio and Flavobacteria and enrichments of multiple antibiotic resistance genes. Additionally, we found a potential link between the host's response to ENR exposure and the intestinal microbiota disorder. Liver metabolites, including phosphatidylcholine, lysophosphatidylcholine, taurocholic acid, and cholic acid, in addition to several metabolic pathways in the liver that are closely linked to the imbalance of intestinal flora were severely maladjusted. These findings suggest that ENR exposure has the potential to negatively affect the gut-liver axis as the primary toxicological mechanism. Our findings provide evidence regarding the negative physiological impacts of antibiotics on marine fish.

The effects of circularly polarized light on mating behavior and gene expression in Anomala corpulenta (Coleoptera: Scarabaeidae).

Light is an important abiotic factor affecting insect behavior. In nature, linearly polarized light is common, but circularly polarized light is rare. Left circularly polarized (LCP) light is selectively reflected by the exocuticle of most scarab beetles, including Anomala corpulenta. Despite our previous research showing that this visual signal probably mediates their mating behavior, the way in which it does so is not well elucidated. In this study, we investigated how LCP light affects not only mating behavior but also gene expression in this species using RNA-seq. The results indicated that disruption of LCP light reflection by females of A. corpulenta probably affects the process by which males of A. corpulenta search for mates. Furthermore, the RNA-seq results showed that genes of the environmental signaling pathways and also of several insect reproduction-related amino acid metabolic pathways were differentially expressed in groups exposed and not exposed to LCP light. This implies that A. corpulenta reproduction is probably regulated by LCP light-induced stress. Herein, the results show that LCP light is probably perceived by males of the species, further mediating their mating behavior. However, this hypothesis needs future verification with additional samples.

Efficient Lithium/Sodium-Ion Storage by Core-Shell Carbon Nanospheres@TiO2 Decorate by Epitaxial WSe2 Nanosheets Derived from Bimetallic Polydopamine Composites.

Metal-polydopamine coordination chemistry attracts great attention owing to the synergistic effect of adjustable components and advantageous structures. However, few efforts have been devoted to exploring bimetal-polydopamine composites, especially for multistructural composites with high-capacity components and high stability. In this regard, the TiO2 @C-WSe2 core-shell nanospheres are designed and fabricated based on Ti-W-polydopamine composites after selenization, in which the TiO2 nanoparticles are encapsulated or embedded in the carbon nanospheres and the external WSe2 nanosheets are grown epitaxially on the carbon surfaces, featuring multiple channels for ion diffusion and abundant active edges for electrochemical reactions. The introduction of WSe2 not only greatly improves the capacity but also results in exponential growth of the active edge. As a result, the as-prepared TiO2 @C-WSe2 displayed long-term cycling performance in lithium-ion batteries. Furthermore, the anode is assembled into sodium-ion batteries, manifesting a stable capacity of 352 mA h g-1 at 1.0 A g-1 even after 2000 cycles, one of the best performances for polydopamine-based composites. Enhanced performance can be attributed to the synergies of high-capacity components and different dimensional materials. This work highlights that the rational design of functional structures provides a novel inspiration for electrodes with effective nanoarchitectures.

Review of the injectate dispersion pattern during anterior quadratus lumborum block.

Since its introduction in 2013, the anterior quadratus lumborum (QL) block (QLB) has been widely used for analgesia in abdominal, pelvic, and hip surgeries, based on the assumption that it has analgesic effects on both incisional and visceral pain. However, the mechanism of the anterior QLB remains unclear, and the results of relevant studies are contradictory. This review aimed to summarize the dispersion patterns of injectates in anterior QLB. We conducted literature searches using PubMed, Cochrane, and Embase databases. A total of 10 cadaveric or radiological studies meeting the inclusion and exclusion criteria were summarized. The dye or contrast agent spread to the thoracic paravertebral space in only 5 of the studies reviewed. Variability in the selection of injection sites relative to the anterior layer of the thoracolumbar fascia during anterior QLB implementation may explain the dispersion difference. The correct injection site of an anterior QLB is anterior to the QL muscle and between the QL muscle and the anterior layer of the thoracolumbar fascia. Further studies are needed to verify the injectate dispersion pattern during anterior QLB.