Variation in the microbiota across different developmental stages of Aedes albopictus is affected by ampicillin exposure.

The microbiota plays an important role in the growth of mosquitoes and the transmission of mosquito-borne pathogens. The effects of changes in aquatic habitats in which mosquitoes live, as one of the major factors closely associated with the microbial communities of mosquitoes, on the microbiota of different developmental stages remain to be elucidated. Here, we compared the microbiota of larvae and pupae of Aedes albopictus exposed to different ampicillin concentrations and investigated the bacterial composition of adult females. The results demonstrate that the microbial community differed substantially between developmental stages and that samples of the same stages shared similarities, whereas differences were observed between adult females. Based on all observations, we hypothesize that the use of ampicillin caused dysbiosis rather than excluding bacteria from mosquitoes and that the disturbing effect of ampicillin was obvious in adults. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that most of the bacteria identified in this study were significantly associated with metabolism. Taken together, our results indicate that ampicillin can change the abundance of bacteria, while microbial communities of Ae. albopictus showed obvious stage-specific characteristics. Further investigations are needed to characterize specific bacterial components that are affected by ampicillin exposure and to quantify their functions, thereby providing a better understanding of the influence of antibiotics on microbial communities at different life stages.

Population movement, city closure and spatial transmission of the 2019-nCoV infection in China

The outbreak of pneumonia caused by a novel coronavirus (2019-nCoV) in Wuhan City of China obtained global concern, the population outflow from Wuhan has contributed to spatial expansion in other parts of China. We examined the effects of population outflow from Wuhan on the 2019-nCoV transmission in other provinces and cities of China, as well as the impacts of the city closure in Wuhan. We observed a significantly positive association between population movement and the number of cases. Further analysis revealed that if the city closure policy was implemented two days earlier, 1420 (95% CI: 1059, 1833) cases could be prevented, and if two days later, 1462 (95% CI: 1090, 1886) more cases would be possible. Our findings suggest that population movement might be one important trigger of the 2019-nCoV infection transmission in China, and the policy of city closure is effective to prevent the epidemic.

Short term effect of PM2.5 on cardiovascular mortality in residents in Changping district, Beijing / 中华流行病学杂志

Objective@#To make a quantitative evaluation on the short term effect of particulate matter with aerodynamic diameter no more than 2.5 μm (PM2.5) on cumulative excess mortality rate (CER) and years of life lost (YLL) in residents in Changping district of Beijing.@*Methods@#The death data in local residents, daily mortality, meteorology data and air pollution data (PM2.5, SO2 and NO2 concentrations) in Changping from 2014 to 2017 were collected. Distributed lag non-linear model was used to assess the age and gender specific cumulative lag effects of PM2.5 on cardiovascular CER and daily YLL in Changping.@*Results@#The effects of PM2.5 on cardiovascular CER and YLL were obvious on lag 7 days and lag 9 days, respectively, peaking on day 14, and lasting for 21 days. On lag0-21 days, for a 10 μg/m3 increase in PM2.5, the population based CER of cardiovascular disease death was 0.021% (95%CI: 0.004%-0.038%), and the YLL was 1.47 (95%CI: 0.23-2.70) years. Greater PM2.5 effect were observed in males and the elderly.@*Conclusion@#PM2.5 increased the risk of cardiovascular disease death and YLL.

Molecular identification of main vector fleas in Qinghai plague foci by DNA barcoding / 中华地方病学杂志

Objective To make up the limitations of traditional morphological classification methods,we identified vector fleas by DNA barcoding in Qinghai Province.Methods The mt DNA cytochrome c oxidase subunit Ⅰ (CO Ⅰ) gene was amplified by PCR from 36 muscle tissues of fleas in 3 states,2 cities and 5 counties of Qinghai Province,and the obtained CO Ⅰ gene fragments were sequenced and aligned.The intra-and inter-species genetic distances were calculated with Mega 6 software using K2-P model and a phylogenetic tree was constructed with neighbor-joining (NJ) method.Results Totally 36 CO Ⅰ gene sequences of 2 superfamilies,4 genera and 6 kinds of vector fleas were measured,the average genetic distance was 0.119,and the intraspecific distance was 0.002-0.027,the interspecific distance was 0.039-0.207,and the interspecific genetic distance was significantly greater than the intraspecific genetic distance.NJ tree showed the same species had formed a single line with high support rate and interspecific branch was clear.Conclusion DNA barcoding is suitable for identification of vector fleas in Qinghai Province,may make up the limitations of traditional morphological classification methods.

Landscape of emerging and re-emerging infectious diseases in China: impact of ecology, climate, and behavior / 医学前沿

For the past several decades, the infectious disease profile in China has been shifting with rapid developments in social and economic aspects, environment, quality of food, water, housing, and public health infrastructure. Notably, 5 notifiable infectious diseases have been almost eradicated, and the incidence of 18 additional notifiable infectious diseases has been significantly reduced. Unexpectedly, the incidence of over 10 notifiable infectious diseases, including HIV, brucellosis, syphilis, and dengue fever, has been increasing. Nevertheless, frequent infectious disease outbreaks/events have been reported almost every year, and imported infectious diseases have increased since 2015. New pathogens and over 100 new genotypes or serotypes of known pathogens have been identified. Some infectious diseases seem to be exacerbated by various factors, including rapid urbanization, large numbers of migrant workers, changes in climate, ecology, and policies, such as returning farmland to forests. This review summarizes the current experiences and lessons from China in managing emerging and re-emerging infectious diseases, especially the effects of ecology, climate, and behavior, which should have merits in helping other countries to control and prevent infectious diseases.

A Survey of Insecticide Resistance in Aedes albopictus (Diptera: Culicidae) During a 2014 Dengue Fever Outbreak in Guangzhou, China.

A dengue fever outbreak in Guangzhou, Guangdong Province, China, in 2014 resulted in ∼37,000 cases and five deaths. Insecticides were sprayed to control the vector of this outbreak, Aedes albopictus (Skuse), a species of mosquito. Aedes albopictus specimens collected from Huadu District (HD), Huangpu District (HP), Luogang District (LG), and Nansha District (NS) in Guangzhou were evaluated using WHO-recommended bioassays for both larvae and adult mosquitoes to determine population resistance to deltamethrin, beta-cypermethrin, cypermethrin, permethrin, dichlorvos, temephos, propoxur, and DDT. Compared with a susceptible laboratory strain of Ae. albopictus (S-lab), all populations showed decreased sensitivities to the eight insecticides, with resistance ratios (RRs) ranging from 2.2 to 275. The RRs were 6.8-275 for pyrethroids, 2.2-4.4 for organophosphates, 5.7-9.0 for carbamates, and 5.3-94.3 for organochlorines. For adult mosquitoes, all populations were sensitive to dichlorvos with 100% mortalities. Mosquitoes from HP, LG, and NS were also sensitive to propoxur. But for other tested insecticides, different degrees of resistance (mortality rate ranging from 11.7% to 94.7%) were observed. Among the four field populations, the resistance levels are presented as follows in descending order: HP > HD > NS > LG. The levels among insecticides classes were pyrethroids > organochlorines > carbamates > organophosphates.

Influence of Bloodmeal Host on Blood Feeding, Egg Production, and Offspring Sex Ratio of Ctenocephalides felis felis (Siphonaptera: Pulicidae).

The cat flea, Ctenocephalides felis felis (Bouché), feeds on different host species, causing annoyance or transmitting disease agents. In this study, the influence of the host of the cat flea on blood feeding, egg production, and sex ratio of the offspring was investigated. Two strains of C. felis were domesticated on either rats or mice for >10 yr in the laboratory, and in this study, these fleas were placed in the following groups and fed on rats or mice continuously: Group A (rat-domesticated C. felis with rats as host); Group B (rat-domesticated C. felis with mice as host); Group C (mouse-domesticated C. felis with rats as host); and Group D (mouse-domesticated C. felis with mice as host). In total, 240 adult fleas were in each group at a sex ratio of female:male = 1.7:1. The mean egg production per flea of Groups A, B, C, and D was 55.0, 19.2, 62.5, and 13.2, respectively. A significant correlation between egg production and the volume of blood consumed was detected for Groups A, B, C, and D. The sex ratio (F:M) of the offspring in Groups A and C was 2.07 and 2.11, respectively, whereas in Groups B and D, the ratio was 1.04 and 1.03, respectively. In conclusion, the C. felis with rats as host consumed more blood, produced more eggs, and had higher sex ratios of the offspring than those with mice as the host.

Analysis of ecological environment factors of Himalayan marmot based on global positioning system, remote sensing and geographical information system in Ynshu City, Qinghai Province / 中华地方病学杂志

Objective To analyze the ecological environmental differences of hibernation hole and temporary hole of Himalayan marmot based on global positioning system (GPS),remote sensing (RS) and geographical information system (GIS) in Yushu City,Qinghai Province.Methods Hibernation holes and temporary holes were located based on GPS.Ecological environment factors related with Himalayan marmot,such as land surface temperature (LST),digital elevation model (DEM),normalized different vegetation index (NDVI),were extracted based on RS and GIS.The ecological environment differences between hibernation hole and temporary hole were explored based on epidemiological statistical method.Results Monthly LST (degree Kelvin,K) of hibernation hole was slightly higher than that of temporary hole in the same period.Yearly day LST of hibernation hole was 1.1 K higher than that of temporary hole in the same period.Yearly night LST of hibernation hole was 0.3 K higher than that of temporary hole in the same period.Monthly NDVI of temporary hole was slightly higher than that of hibernation hole in the same period.Yearly NDVI of temporary hole was 0.001 higher than that of hibernation hole in the same period.DEM of temporary hole was about 40 m higher than that of hibernation hole.The top two ratios of soil type factors were both alpine meadow soil and dark felty soil for hibernation hole and temporary hole,which were 62.1％ and 29.3％,64.4％ and 25.6％.The top two ratios of land cover factors were both rainfed cropland and mosaic cropland for hibernation hole and temporary hole,which were 43.3％ and 25.8％,40.0％ and 26.2％.Conclusions Soil type factors and land cover factors for hibernation hole and temporary hole are similar.Lower DEM and higher LST are chose for hibernation hole,and better NDVI is chose for temporary hole,which is good for food intake.

Influence of daily ambient temperature on mortality and years of life lost in Chongqing / 中华流行病学杂志

<p><b>OBJECTIVE</b>To evaluate the influence of extreme ambient temperature on mortality and years of life lost (YLL) in Chongqing.</p><p><b>METHODS</b>The daily mortality, meteorology and air pollution index data in Chongqing from the 1(st) January 2010 to the 31(st) December 2013 were collected. Distributed lag non-linear model (DLNM) was used to assess the influence of daily ambient temperature on daily number of deaths and daily YLL respectively. The delayed and cumulative effects of extreme temperature on sex, age, and cause-specific mortality were also assessed.</p><p><b>RESULTS</b>The relationships between ambient temperature and non-accidental, cardiovascular disease and respiratory disease mortalities and YLL were U-shaped or W-shaped. The effect of heat was obvious on that day, peaked on day 7, and lasted for two weeks, whereas the effect of cold was obvious a week later and lasted for a month. As 1 ℃ increase of ambient temperature, the cumulative relative risks (CRR) of high temperature across lag 0-7 days on non-accidental, respiratory disease and cardiovascular disease mortalities were 1.05 (95%CI: 1.03-1.07), 1.08 (95%CI: 1.05-1.11) and 1.05 (95%CI: 1.01-1.09) respectively. The effects of heat on YLL for each cause were 23.81 (95%CI: 12.31-35.31), 14.34 (95%CI: 8.98-19.70) and 4.43 (95%CI: 1.64-7.21), respectively. On cold days, 1 ℃ decrease of ambient temperature was correlated with an increase in CRR of 1.06 (95%CI: 1.04-1.08), 1.09 (95%CI:1.06-1.12) and 1.06 (95%CI: 1.02-1.11) from lag 0 to 14 for non-accidental, respiratory disease and cardiovascular disease mortalities, respectively. The estimated YLL were 23.34 (95%CI: 10.04-36.64), 16.39 (95%CI: 10.19-22.59) and 2.61 (95%CI: -0.61-5.82). People aged ≥65 years tend to have higher CRR and YLL than those aged <65 years. On high temperature days, the CRR in women was higher than that in men, while the YLL in women was lower than that in men. On low temperature days, both the CRR and YLL in women were higher than those in men.</p><p><b>CONCLUSIONS</b>Both high and low ambient temperature have adverse health effects. People aged ≥65 years are more sensitive to both high and low ambient temperature. Younger men are more sensitive to high ambient temperature and women and elder men are sensitive to low ambient temperature. It is necessary to take targeted measures to protect the population in Chongqing from the adverse influence of extreme ambient temperature.</p>

Impact of temperature on non-accidental deaths and cause-specific mortality in four districts of Jinan / 中华流行病学杂志

Objective To study the relationship between daily temperature and non-accidental deaths in four districts of Jinan,and to investigate the impact of temperature on cause-specific mortality. Methods Data on daily mortality of the four districts(Shizhong,Huaiyin,Tianqiao,Lixia) as well as data related to meteorology and air pollution index were collected from January 1,2008 to December 31,2012. Distributed lag non-linear model(DLNM)was then used to assess the effects of temperature on all non-accidental deaths and deaths caused by cardiovascular diseases (CVD), respiratory diseases(RD),digestive diseases,urinary diseases,and also subcategories to hypertension, ischemic heart diseases(IHD),acute myocardial infarction(AMI),cerebro-vascular diseases(CBD) and chronic lower respiratory diseases. Results A W-shaped relationship was noticed between daily average temperature and non-accidental deaths. The effect of low temperature last for more than 30 days,much longer than that of high temperature,in which presented a harvesting effect less than 5 days. As to the cause-specific mortality,short-term heat effects were seen in CVD and RD as well as related subgroups as IHD,CBD and AMI,with RRs at lag 0 as 1.12(95%CI:1.07-1.17),1.06 (95%CI:1.02-1.31),1.08(95%CI:1.003-1.16),1.10(95%CI:1.02-1.20) and 1.13 (95%CI:1.003-1.26). Relatively higher RRs were seen in urinary diseases and hypertension under extremely high temperature,reaching as high as 2.30(95%CI:1.18-4.51)and 1.65(95%CI:1.02-2.69). Cold weather presented a delayed effect for 30 days,with cumulative RRs as 1.51(95%CI:1.42-1.60),1.90 (95%CI:1.64-2.20),2.12(95%CI:1.67-2.69),1.48(95%CI:1.08-2.03),1.60(95%CI:1.46-1.75), 1.40(95%CI:1.26-1.55),1.68(95%CI:1.45-1.95)for CVD,RD,chronic lower respiratory diseases, hypertension,IHD,CBD and AMI,on sequence. Conclusion A relationship was seen between daily temperature and non-accidental deaths as well as cause-specific mortality. Either high or low temperature seemed to be detrimental. Related measures on disease prevention should be taken during the cold and hot seasons.

Heat vulnerability assessment in Jinan city:a comparison between residents living in urban centers and urban-fringe areas / 中华流行病学杂志

Objective To find out the differences in regional characteristics of heat vulnerability between people living in urban centers and urban-fringe areas of Jinan city so as to provide basis for the development of adaptation measures to heat. Methods A cross-sectional survey on heat vulnerability was conducted in urban center and urban-fringe areas of Jinan city,using a self-designed questionnaire among 801 residents at the age of 16 years or older in August 2013. Data of 23 indicators related to heat vulnerability were collected and aggregated to 7 dimensions:health and medical insurance,social networks,heat perception and adaptive behavior,economic status,resources, living environment and working environment. An index score was calculated using a balanced weighted average approach for each dimension,ranging from 0 to 1,with the closer to 1 as greater vulnerability. Results The scores on heat perception and adaptive behavior,economic status, resources and working environment dimensions for urban-fringe areas were 0.42,0.63,0.55 and 0.62, statistically significantly higher than the urban center area of 0.41,0.51,0.26 and 0.41. Scores of living environment,social networks and health/medical insurance dimensions for urban center area were 0.57,0.49 and 0.31,which were all higher than the urban-fringe areas of 0.50,0.46 and 0.25, with differences statistically significant. Conclusion Residents living in the urban center might be more vulnerable to heat in terms of living environment,health/medical insurance and social networks while residents living in the urban-fringe areas might more be vulnerable in terms of heat perception and adaptive behavior,economic status,life resources and working environment. These facts indicated that heat vulnerability among residents could be quite different,even at a fine geographic sale. We would thus suggest that intervention strategies on protecting people from heat,should be more targeted.

Impact of temperature on non-accidental deaths and cause-specific mortality in four districts of Jinan / 中华流行病学杂志

Objective To study the relationship between daily temperature and non-accidental deaths in four districts of Jinan,and to investigate the impact of temperature on cause-specific mortality. Methods Data on daily mortality of the four districts(Shizhong,Huaiyin,Tianqiao,Lixia) as well as data related to meteorology and air pollution index were collected from January 1,2008 to December 31,2012. Distributed lag non-linear model(DLNM)was then used to assess the effects of temperature on all non-accidental deaths and deaths caused by cardiovascular diseases (CVD), respiratory diseases(RD),digestive diseases,urinary diseases,and also subcategories to hypertension, ischemic heart diseases(IHD),acute myocardial infarction(AMI),cerebro-vascular diseases(CBD) and chronic lower respiratory diseases. Results A W-shaped relationship was noticed between daily average temperature and non-accidental deaths. The effect of low temperature last for more than 30 days,much longer than that of high temperature,in which presented a harvesting effect less than 5 days. As to the cause-specific mortality,short-term heat effects were seen in CVD and RD as well as related subgroups as IHD,CBD and AMI,with RRs at lag 0 as 1.12(95%CI:1.07-1.17),1.06 (95%CI:1.02-1.31),1.08(95%CI:1.003-1.16),1.10(95%CI:1.02-1.20) and 1.13 (95%CI:1.003-1.26). Relatively higher RRs were seen in urinary diseases and hypertension under extremely high temperature,reaching as high as 2.30(95%CI:1.18-4.51)and 1.65(95%CI:1.02-2.69). Cold weather presented a delayed effect for 30 days,with cumulative RRs as 1.51(95%CI:1.42-1.60),1.90 (95%CI:1.64-2.20),2.12(95%CI:1.67-2.69),1.48(95%CI:1.08-2.03),1.60(95%CI:1.46-1.75), 1.40(95%CI:1.26-1.55),1.68(95%CI:1.45-1.95)for CVD,RD,chronic lower respiratory diseases, hypertension,IHD,CBD and AMI,on sequence. Conclusion A relationship was seen between daily temperature and non-accidental deaths as well as cause-specific mortality. Either high or low temperature seemed to be detrimental. Related measures on disease prevention should be taken during the cold and hot seasons.

Heat vulnerability assessment in Jinan city:a comparison between residents living in urban centers and urban-fringe areas / 中华流行病学杂志

Objective To find out the differences in regional characteristics of heat vulnerability between people living in urban centers and urban-fringe areas of Jinan city so as to provide basis for the development of adaptation measures to heat. Methods A cross-sectional survey on heat vulnerability was conducted in urban center and urban-fringe areas of Jinan city,using a self-designed questionnaire among 801 residents at the age of 16 years or older in August 2013. Data of 23 indicators related to heat vulnerability were collected and aggregated to 7 dimensions:health and medical insurance,social networks,heat perception and adaptive behavior,economic status,resources, living environment and working environment. An index score was calculated using a balanced weighted average approach for each dimension,ranging from 0 to 1,with the closer to 1 as greater vulnerability. Results The scores on heat perception and adaptive behavior,economic status, resources and working environment dimensions for urban-fringe areas were 0.42,0.63,0.55 and 0.62, statistically significantly higher than the urban center area of 0.41,0.51,0.26 and 0.41. Scores of living environment,social networks and health/medical insurance dimensions for urban center area were 0.57,0.49 and 0.31,which were all higher than the urban-fringe areas of 0.50,0.46 and 0.25, with differences statistically significant. Conclusion Residents living in the urban center might be more vulnerable to heat in terms of living environment,health/medical insurance and social networks while residents living in the urban-fringe areas might more be vulnerable in terms of heat perception and adaptive behavior,economic status,life resources and working environment. These facts indicated that heat vulnerability among residents could be quite different,even at a fine geographic sale. We would thus suggest that intervention strategies on protecting people from heat,should be more targeted.

DNA barcoding of rodent in Ningxia Hui Autonomous Region,China / 中国人兽共患病学报

ABSTRACT:To improve the ability of correct classification and identification of rodents in different kinds of foci of zoonosis in Ningxia Hui Autonomous Region in China ,rodents samples from different habitats of Ningxia were collected .Sequences of COI gene were amplified and sequenced from 154 samples .Based on these sequences ,the pairwise genetic distance were calcu‐lated ,and a Neighbor Joining tree were constructed .According to the NJ tree ,20 clusters with high bootstrap support were found from 19 morphological species .The striped dwarf hamsters were divided into two clusters ,which suggested that there were two cryptic species with stripe on the back .The pika from Helan Mountain showed close relationship with Ochotona pal‐lasi ,and the genetic distance was as low as 3 .6% .Results show that DNA barcodes could be used to accurately identify speci‐mens of rodents and correct morphological identification errors .It could discover appearance indistinguishable implied species and could better study the classification and evolution of rodents .

Heat vulnerability assessment in Jinan city: a comparison between residents living in urban centers and urban-fringe areas / 中华流行病学杂志

<p><b>OBJECTIVE</b>To find out the differences in regional characteristics of heat vulnerability between people living in urban centers and urban-fringe areas of Jinan city so as to provide basis for the development of adaptation measures to heat.</p><p><b>METHODS</b>A cross-sectional survey on heat vulnerability was conducted in urban center and urban-fringe areas of Jinan city, using a self-designed questionnaire among 801 residents at the age of 16 years or older in August 2013. Data of 23 indicators related to heat vulnerability were collected and aggregated to 7 dimensions:health and medical insurance, social networks, heat perception and adaptive behavior, economic status, resources, living environment and working environment. An index score was calculated using a balanced weighted average approach for each dimension, ranging from 0 to 1, with the closer to 1 as greater vulnerability.</p><p><b>RESULTS</b>The scores on heat perception and adaptive behavior, economic status, resources and working environment dimensions for urban-fringe areas were 0.42,0.63,0.55 and 0.62, statistically significantly higher than the urban center area of 0.41,0.51,0.26 and 0.41. Scores of living environment, social networks and health/medical insurance dimensions for urban center area were 0.57,0.49 and 0.31, which were all higher than the urban-fringe areas of 0.50,0.46 and 0.25, with differences statistically significant.</p><p><b>CONCLUSION</b>Residents living in the urban center might be more vulnerable to heat in terms of living environment, health/medical insurance and social networks while residents living in the urban-fringe areas might more be vulnerable in terms of heat perception and adaptive behavior, economic status, life resources and working environment. These facts indicated that heat vulnerability among residents could be quite different, even at a fine geographic sale. We would thus suggest that intervention strategies on protecting people from heat, should be more targeted.</p>

Impact of temperature on non-accidental deaths and cause-specific mortality in four districts of Jinan / 中华流行病学杂志

<p><b>OBJECTIVE</b>To study the relationship between daily temperature and non-accidental deaths in four districts of Jinan, and to investigate the impact of temperature on cause-specific mortality.</p><p><b>METHODS</b>Data on daily mortality of the four districts (Shizhong, Huaiyin, Tianqiao, Lixia) as well as data related to meteorology and air pollution index were collected from January 1, 2008 to December 31, 2012. Distributed lag non-linear model (DLNM) was then used to assess the effects of temperature on all non-accidental deaths and deaths caused by cardiovascular diseases (CVD), respiratory diseases (RD), digestive diseases, urinary diseases, and also subcategories to hypertension, ischemic heart diseases (IHD), acute myocardial infarction (AMI), cerebro-vascular diseases (CBD) and chronic lower respiratory diseases.</p><p><b>RESULTS</b>A W-shaped relationship was noticed between daily average temperature and non-accidental deaths. The effect of low temperature last for more than 30 days, much longer than that of high temperature, in which presented a harvesting effect less than 5 days. As to the cause-specific mortality, short-term heat effects were seen in CVD and RD as well as related subgroups as IHD, CBD and AMI, with RRs at lag 0 as 1.12 (95%CI:1.07-1.17), 1.06 (95% CI:1.02-1.31), 1.08 (95% CI:1.003-1.16), 1.10 (95% CI:1.02-1.20) and 1.13 (95% CI: 1.003-1.26). Relatively higher RRs were seen in urinary diseases and hypertension under extremely high temperature, reaching as high as 2.30 (95%CI:1.18-4.51) and 1.65 (95%CI:1.02-2.69). Cold weather presented a delayed effect for 30 days, with cumulative RRs as 1.51 (95%CI:1.42-1.60), 1.90 (95%CI:1.64-2.20), 2.12 (95%CI:1.67-2.69), 1.48 (95%CI:1.08-2.03), 1.60 (95%CI:1.46-1.75), 1.40 (95%CI:1.26-1.55), 1.68 (95%CI:1.45-1.95) for CVD, RD, chronic lower respiratory diseases, hypertension,IHD, CBD and AMI, on sequence.</p><p><b>CONCLUSION</b>A relationship was seen between daily temperature and non-accidental deaths as well as cause-specific mortality. Either high or low temperature seemed to be detrimental. Related measures on disease prevention should be taken during the cold and hot seasons.</p>

Permanent genetic resources added to Molecular Ecology Resources Database 1 June 2012-31 July 2012.

This article documents the addition of 96 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Clarias batrachus, Marmota himalayana, Schizothorax richardsonii, Sitophilus zeamais and Syagrus romanzoffiana. These loci were cross-tested on the following species: Clarias dussumeri, Clarias gariepinus, Heteropneustus fossilis, Sitophilus granarius and Sitophilus oryzae.