ABSTRACT
Though it is known that the immune system exerts some influence on the resistance against T. cruzi infection its precise role in this process is not well-understood. Some IL-1B alleles and haplotypes have been associated with susceptibility to inflammatory, autoimmune and infectious diseases. The objective of this study was to determine and compare the distribution of IL-1B and IL-1 receptor antagonist (IL-1RN) polymorphisms among T. cruzi seropositive patients, patients with idiopathic dilated cardiomyopathy (IDC) and healthy individuals. We studied 86 individuals seropositive for T. cruzi (58 patients with chronic chagasic cardiomyopathy (CCC) and 28 asymptomatics), 50 seronegative individuals with IDC and 109 healthy individuals. IL-1B-511, IL-1F10.3 IL-1RN.4, IL-1RN 6/1, and IL-1RN 6/2 polymorphisms were analyzed using real-time PCR allelic discrimination technology. Infected patients presented an increased frequency of the CC genotype of the IL-1RN.4 polymorphism when compared to IDC (pC = 0.028; OR = 11.46). The C allele of this polymorphism was found increased in CCC when compared with IDC (pC = 0.036; OR = 0.5) and with controls (pC = 0.035; OR = 1.87). CC genotype of IL-1RN.4 polymorphism was increased in patients with CCC when compared to IDC (pC = 0.0018; OR = 16.74) and healthy individuals (pC = 0.011; OR = 3.6). There is an evident association between the IL1RN.4 polymorphism, T. cruzi infection and CCC development.
Subject(s)
Chagas Cardiomyopathy/genetics , Genetic Predisposition to Disease , Interleukin 1 Receptor Antagonist Protein/genetics , Interleukin-1beta/genetics , Cardiomyopathy, Dilated/genetics , Chagas Disease/genetics , Female , Gene Frequency , Genome-Wide Association Study , Genotype , Humans , Male , Middle Aged , Polymorphism, Single NucleotideABSTRACT
To test for an association with risk for restenosis after coronary stent placement, the TNF-alpha and IL-10 polymorphisms were analyzed by 5' exonuclease TaqMan assays in 162 patients who initially underwent coronary stenting. Analysis of basal and procedure coronary angiographies revealed a higher proportion of restenosis in lesions treated with bare metal stents compared with those treated with drug-eluting stents (P < 0.001). Distribution of TNF-alpha genotypes was similar in patients with and without restenosis. The IL-10 polymorphisms showed a moderate protective trend of the -819 TT genotype against restenosis when the lesions were analyzed (P = 0.071, OR = 0.471). Multivariate analysis confirmed a protective role for drug-eluting stents (P < 0.001, OR = 0.199) and the -819 TT genotype (P = 0.037, OR = 0.391). These results suggest the IL-10 -819 TT genotype has a protective role against in-stent restenosis.
Subject(s)
American Indian or Alaska Native/genetics , Coronary Restenosis/genetics , Interleukin-10/genetics , Polymorphism, Genetic , Promoter Regions, Genetic/genetics , Stents/adverse effects , Tumor Necrosis Factor-alpha/genetics , Coronary Angiography , Coronary Restenosis/diagnostic imaging , Coronary Restenosis/etiology , Female , Gene Frequency , Genetic Predisposition to Disease , Genotype , Humans , Male , Mexico , Middle Aged , Postoperative Complications/diagnostic imaging , Postoperative Complications/etiologyABSTRACT
Se estudió la presencia de Antígeno y Anticuerpo anti VIH en 129 muestras de suero provenientes de reos, prostitutas y homosexuales de la VI Región del país, siendo el total de las muestras negativas para ambos marcadores.
Subject(s)
Child , Adolescent , Adult , Middle Aged , Humans , Male , Female , Risk Groups , Immunologic Deficiency Syndromes/diagnosis , Chile , Enzyme-Linked Immunosorbent Assay , Homosexuality , Prisoners , Sex WorkABSTRACT
Mediante un estudio abierto, se estudió la eficacia clínica y la tolerancia para tolciclato, en 31 niños con diferentes dermatomicosis, todos menores de 12 años de edad. Las principales dermatomicosis fueron tiñas circinadas (n = 19), tiña cruris (n = 1), tiña palmar (n = 2), pitiriasis versicolor (n = 7) y micosis interortejo (n = 2). En 90% de los casos se demostró desaparición de la evidencia de infección en los exámenes directos y cultivos para hongos y en 94% desaparición de las manifestaciones clínicas. No se observaron síntomas ni signos de reacciones adversas en esta serie