Do Concurrent Peri-Implantitis and Periodontitis Share Their Microbiotas? A Pilot Study.

The microbial compositions from concurrent peri-implant and periodontal lesions were compared, since the results reported in the literature on the etiological relationship between these oral pathologies are contradictory. Microbial compositions from nine patients were evaluated using Illumina MiSeq sequencing of 16S rRNA gene amplicons and Principal Components Analysis. Comparisons between the use of curettes or paper points as collection methods and between bacterial composition in both pathologies were performed. Paper points allowed the recovery of a higher number of bacterial genera. A higher bacterial diversity was found in peri-implantitis compared to periodontal samples from the same patient, while a greater number of operational taxonomic units (OTUs) were present in the corresponding periodontal samples. A higher abundance of oral pathogens, such as Porphyromonas or Treponema, was found in peri-implantitis sites. The opposite trend was observed for Aggregatibacter abundance, which was higher in periodontal than in peri-implantitis lesions, suggesting that both oral pathologies could be considered different but related diseases. Although the analysis of a higher number of samples would be needed, the differences regarding the microbial composition provide a basis for further understating the pathogenesis of peri-implant infections.

Time-loss Injuries among Female and Male Spanish Rink Hockey Players.

The present study describes the time-loss injuries among female and male athletes of the Spanish rink hockey league during the 2021/22 season.We performed a retrospective cohort study on time-loss injuries, whereby the athlete is prevented from participating in a training session or game because of the injury.A total of 463 athletes were included, with 326 (70.4%) senior male and 137 (29.6%) female. Two hundred and eighty-two time-loss injuries were recorded, the most common form being muscle injuries (112 episodes, 39.7%), especially those affecting the hip adductor muscles (52 episodes, 46.4% of muscle injuries). Most injuries were classified as mild (1-7 days of time-loss) and the median return-to-play was 9.5 days (range 1-180).Injury patterns were compared according to gender, position and moment: the results showed significant differences between senior males and females, between field players and goalkeepers, as well as between training and game, in terms of injury nature and type. The injury incidence proportion was significantly higher for field players compared to goalkeepers), and senior males had a significantly higher risk than senior females.The present study provides a starting point for studying and preventing injuries in rink hockey athletes.

An FPGA Based Tracking Implementation for Parkinson's Patients.

This paper presents a study on the optimization of the tracking system designed for patients with Parkinson's disease tested at a day hospital center. The work performed significantly improves the efficiency of the computer vision based system in terms of energy consumption and hardware requirements. More specifically, it optimizes the performances of the background subtraction by segmenting every frame previously characterized by a Gaussian mixture model (GMM). This module is the most demanding part in terms of computation resources, and therefore, this paper proposes a method for its implementation by means of a low-cost development board based on Zynq XC7Z020 SoC (system on chip). The platform used is the ZedBoard, which combines an ARM Processor unit and a FPGA. It achieves real-time performance and low power consumption while performing the target request accurately. The results and achievements of this study, validated in real medical settings, are discussed and analyzed within.

Three-D Wide Faces (3DWF): Facial Landmark Detection and 3D Reconstruction over a New RGB⁻D Multi-Camera Dataset.

Latest advances of deep learning paradigm and 3D imaging systems have raised the necessity for more complete datasets that allow exploitation of facial features such as pose, gender or age. In our work, we propose a new facial dataset collected with an innovative RGB⁻D multi-camera setup whose optimization is presented and validated. 3DWF includes 3D raw and registered data collection for 92 persons from low-cost RGB⁻D sensing devices to commercial scanners with great accuracy. 3DWF provides a complete dataset with relevant and accurate visual information for different tasks related to facial properties such as face tracking or 3D face reconstruction by means of annotated density normalized 2K clouds and RGB⁻D streams. In addition, we validate the reliability of our proposal by an original data augmentation method from a massive set of face meshes for facial landmark detection in 2D domain, and by head pose classification through common Machine Learning techniques directed towards proving alignment of collected data.

[Validation of the Montreal Cognitive Assessment (MoCA) as a screening tool for mild cognitive impairment in the population of Buenos Aires, Argentina]. / Validez de la prueba de Evaluación Cognitiva de Montreal (MoCA) como una herramienta de screening para detectar Deterioro Cognitivo Leve en población de Buenos Aires, Argentina.

INTRODUCTION: The Montreal Cognitive Assessment (MoCA) test is a brief tool for neuropsychological assessment. OBJECTIVE: to validate the MoCAin the population of Buenos Aires, Argentina, to allow for the use of the test for the detection of Mild Cognitive Impairment (MCI). METHODS: The sample consisted of 269 adults over 60 years old and of schooling of more than 6 years (healthy adults n = 115 and MCIn = 154). Receiver operating characteristic (ROC) analysis was used to establish the relationship between the diagnoses of the patients and the scores obtained at MoCA. The optimal cut-off points were selected, and the positive and negative predictive value were calculated for them. RESULTS: The area under the curve (AUC) was 0,741 (p <0001, 95% CI:.682 -.800) for the MMSE and 0.810 (p <0001, 95% CI:.759 -. 861) for the MoCA test. The cut point suggested using the MoCA test is 26 points, which throws .727 of sensitivity and a specificity of. 748. CONCLUSION: The MoCA test is a useful test for clinical consultation. Its brevity and simplicity place it as an interesting instrument for neuropsychological screening in the Argentinian population.

ICT Services for Life Improvement for the Elderly.

Integrated care ICT Platform to support patients, care-givers and health/social professionals in the care of dementia and Parkinson's disease with training, empowerment, sensor-based data analysis and cooperation services based on user-friendly interfaces.

Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice.

Several glycoproteins in mammalian brains contain α2,8-linked disialic acid residues. We previously showed a constant expression of disialic acid (DiSia) in the hippocampus, olfactory bulb and cortex, and a gradual decrease of expression in the cerebellum from neonatal to senile mice. Previous publications indicate that neurite extension of neuroblastoma-derived Neuro2A cells is inhibited in the presence of DiSia antibody. Based on this, we treated Neuro2A cell cultures with RNA interference for ST8SiaIII mRNA, the enzyme responsible for DiSia formation. We observed that neurite extension was inhibited by this treatment. Taking this evidence into consideration and the relationship of the cerebellum with learning and memory, we studied the role of DiSia expression in a learning task. Through delivery of pST8SiaIII into the brains of C57BL/6 neonatal mice, we inhibited the expression of ST8SiaIII. ST8SiaIII mRNA and protein expressions were analyzed by real-time PCR and western blot, respectively. In this work, we showed that pST8SiaIII-treated mice presented a significantly reduced level of ST8SiaIII mRNA in the cerebellum (p<0.01) in comparison to control mice at 8 days after treatment. It is also noted that these levels returned to baseline values in the adulthood. Then, we evaluated behavioural performance in the T-Maze, a learning task that estimates procedural memory. At all ages, pST8SiaIII-treated mice showed a lower performance in the test session, being most evident at older ages (p<0.001). Taken all together, we conclude that gene expression of ST8SiaIII is necessary for some cognitive tasks at early postnatal ages, since reduced levels impaired procedural memory in adult mice.

Efectos del destete temprano sobre el comportamiento adulto en un modelo animal / Early weaning and adult behavioral disorders in an animal model

El destete temprano ha demostrado ser un factor que disminuye la resistencia orgánica de los animales contra condiciones adversas. La prueba de natación forzada se diseñó para experimentar la defensa del animal frente a un estímulo estresante. La conducta activa como el escalar se interpreta como una modelización experimental de voluntad o capacidad activa de escape. Bajo la hipótesis de que el destete temprano podría tener manifestaciones en la vida adulta relacionadas con la defensa frente al estrés, nuestro objetivo fue analizar la conducta de escalado o escape, durante una prueba de natación forzada,en animales adultos que habían sido destetados precozmente. Se utilizaron 20 ratas Wistar (4 meses de edad), divididas en dos grupos: G1 con destete temprano y G2 control. La unidad de análisis o tip, se refirió a la cantidad de veces que el animal presentó la conducta de escape a lo largo de 5 minutos. Se hallaron diferencias estadísticamente significativas entre los tips de G1 y G2 (15,8 más menos 4,15 contra 21,4 más menos 4,14, p< 0,05). Por otra parte, se encontró también una diferencia significativa entre las ratas hembra y los machos. El presente estudio permitiría elucidar experimentalmente las acciones que en la vida adulta del individuo ocasionan alteraciones tempranas en los vínculos primarios.(AU)

Early weaning (EW) has proven to be a factor that decreases the animals organic resistance against adverse conditions. Climbing in the forced swimming test (FST) is the active behavior that expresses the will of the rat to escape the stressful situation. A possible association between these two stress factors (EW and FST) has not yet been reported as a plausible model for the study of stress and depression. Under the working hypothesis that EW has possible adult behavioral correlateswhen associated with the FST, our objective was to analyze the levels of alteration in the active behaviors among groups. 20 Wistar rats (4 month old), were divided into two groups, the early weaned group (G1) and a control group (G2). The unit of analysis (tip), was defined as the number of intended escapes the animal performs in five minutes. We have found the differences stated by our hypothesis, comparing the frequency of scale or escape (tips), from a group with EW to a control group (15.8 ± 4.15 vs. 21.4 ± 4.14, p< 0.05). In turn, we found that, among females, the difference was significantly wider.The present study could elucidate under experimental conditions the effect that in adult behavior induces an alteration inthe more primary bonds.(AU)

Efectos del destete temprano sobre el comportamiento adulto en un modelo animal / Early weaning and adult behavioral disorders in an animal model

El destete temprano ha demostrado ser un factor que disminuye la resistencia orgánica de los animales contra condiciones adversas. La prueba de natación forzada se diseñó para experimentar la defensa del animal frente a un estímulo estresante. La conducta activa como el escalar se interpreta como una modelización experimental de voluntad o capacidad activa de escape. Bajo la hipótesis de que el destete temprano podría tener manifestaciones en la vida adulta relacionadas con la defensa frente al estrés, nuestro objetivo fue analizar la conducta de escalado o escape, durante una prueba de natación forzada,en animales adultos que habían sido destetados precozmente. Se utilizaron 20 ratas Wistar (4 meses de edad), divididas en dos grupos: G1 con destete temprano y G2 control. La unidad de análisis o tip, se refirió a la cantidad de veces que el animal presentó la conducta de escape a lo largo de 5 minutos. Se hallaron diferencias estadísticamente significativas entre los tips de G1 y G2 (15,8 más menos 4,15 contra 21,4 más menos 4,14, p< 0,05). Por otra parte, se encontró también una diferencia significativa entre las ratas hembra y los machos. El presente estudio permitiría elucidar experimentalmente las acciones que en la vida adulta del individuo ocasionan alteraciones tempranas en los vínculos primarios.

Early weaning (EW) has proven to be a factor that decreases the animal’s organic resistance against adverse conditions. Climbing in the forced swimming test (FST) is the active behavior that expresses the will of the rat to escape the stressful situation. A possible association between these two stress factors (EW and FST) has not yet been reported as a plausible model for the study of stress and depression. Under the working hypothesis that EW has possible adult behavioral correlateswhen associated with the FST, our objective was to analyze the levels of alteration in the active behaviors among groups. 20 Wistar rats (4 month old), were divided into two groups, the early weaned group (G1) and a control group (G2). The unit of analysis (tip), was defined as the number of intended escapes the animal performs in five minutes. We have found the differences stated by our hypothesis, comparing the frequency of scale or escape (tips), from a group with EW to a control group (15.8 ± 4.15 vs. 21.4 ± 4.14, p< 0.05). In turn, we found that, among females, the difference was significantly wider.The present study could elucidate under experimental conditions the effect that in adult behavior induces an alteration inthe more primary bonds.

[Early weaning and adult behavioral disorders in an animal model]. / Efectos del destete temprano sobre el comportamiento adulto en un modelo animal.

Early weaning (EW) has proven to be a factor that decreases the animal's organic resistance against adverse conditions. Climbing in the forced swimming test (FST) is the active behavior that expresses the will of the rat to escape the stressful situation. A possible association between these two stress factors (EW and FST) has not yet been reported as a plausible model for the study of stress and depression. Under the working hypothesis that EW has possible adult behavioral correlates when associated with the FST, our objective was to analyze the levels of alteration in the active behaviors among groups. 20 Wistar rats (4 month old), were divided into two groups, the early weaned group (G1) and a control group (G2). The unit of analysis (tip), was defined as the number of intended escapes the animal performs in five minutes. We have found the differences stated by our hypothesis, comparing the frequency of scale or escape (tips), from a group with EW to a control group (15.8 +/- 4.15 vs. 21.4 +/- 4.14, p< 0.05). In turn, we found that, among females, the difference was significantly wider. The present study could elucidate under experimental conditions the effect that in adult behavior induces an alteration in the more primary bonds.

A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation.

Stem cells were derived from hatched blastocyst-stage mouse embryos of the C57BL/6 strain employing a knockout serum replacement instead of the traditional fetal calf serum, thereby avoiding the use of immunosurgery. Although fetal calf serum was not good for isolation of stem cells, a combination of this serum plus knockout serum increased the expansion rate of the cell culture. The derived cells were capable of maintaining an undifferentiated state during several passages, as demonstrated by the presence of alkaline phosphatase activity, stage-specific embryonic antigen 1 (SSEA-1), and octamer binding protein 4 (Oct-4). Suspension culture in bacteriological dishes gave better results than the hanging drop method for differentiation by means of embryoid body formation. Mouse embryonic stem cells showed spontaneous differentiation into derivatives of the 3 germ layers in culture media supplemented with fetal calf serum but not with knockout serum.