ABSTRACT
Phylogenetic studies with Zika virus (ZIKV) have been conducted in Brazil. In this study, we sequenced 8 new sequences of the ZIKV envelope (E) gene from strains of cases from the Paraná and Mato Grosso do Sul states in 2016. A low phylogenetic signal was observed, with more than 40% of unresolved quartets, and the Maximum Likelihood Tree grouped all sequences in the Brazilian branches within the Asian genotype. In addition, a Shannon entropy analysis was conducted, showing a high stability in the E protein through the ZIKV polyprotein. Taken together, these results suggest a high degree of conservation in the ZIKV E gene from the recent American outbreaks.
Subject(s)
Disease Outbreaks , Viral Envelope Proteins/genetics , Zika Virus Infection/virology , Zika Virus/genetics , Brazil/epidemiology , Genes, Viral , Genome, Viral , Genotype , Humans , Viral Envelope/metabolismABSTRACT
kin17 has been described as a protein involved in the processes of DNA replication initiation, DNA recombination, and DNA repair. kin17 has been studied as a potential molecular marker of breast cancer. This work reports the detection and localization of this protein in the murine melanoma cell line B16F10-Nex2 and in two derived subclones with different metastatic potential, B16-8HR and B16-10CR. Nuclear and chromatin-associated protein fractions were analyzed, and kin17 was detected in all fractions, with an elevated concentration observed in the chromatin-associated fraction of the clone with low metastatic potential, suggesting that the kin17 expression level could be a marker of melanoma.
Subject(s)
DNA-Binding Proteins/metabolism , Melanoma/metabolism , Nuclear Proteins/metabolism , Animals , Cell Movement , Chromatin/metabolism , DNA Repair , DNA Replication , Isografts , Melanoma/genetics , Melanoma/pathology , Melanoma, Experimental , Mice , Neoplasm MetastasisABSTRACT
ABSTRACT Urban ants are considered a serious problem for public health due to their capacity to occupy all environments and for their being mechanical vectors of microorganisms. The present work was aimed to verify the occurrence of ant species at a hospital, at the General Office of Health, and at a laboratory of clinical analysis, in the county of Bandeirantes, PR, Brazil. A total of 1,629 collections were made in the period from October to December 2007, using nonpoisonous baits. The species recorded were: Tapinoma melanocephalum (Fabricius, 1793); Monomorium pharaonis (Linnaeus, 1758), Paratrechina fulva (Mayr, 1862), Pheidole triconstricta Forel, 1886, Pheidole sp. 1, Pheidole sp. 2, Pheidole sp. 3, Pheidole sp. 4, Camponotus atriceps (Fr. Smith, 1858), Brachymyrmex sp., and Dorymyrmex sp. The microorganisms included the bacteria Staphylococcus sp., Serratia sp., Klebsiella sp., Escherichia coli, Salmonella sp., and Pseudomonas sp.; the fungi Cladosporium sp., Fusarium sp., Trichophyton sp., Aspergillus sp. and Penicillium sp.; as well as yeasts.
RESUMO As formigas urbanas são consideradas um problema grave na saúde pública pela capacidade de ocupar todos os ambientes e por serem vetores mecânicos de micro-organismos. O presente trabalho teve por objetivo verificar as espécies que ocorrem em estabelecimentos ligados a área da saúde, bem como os microrganismos veiculados por essas espécies. Em um total de 1.629 coletas realizadas de outubro a dezembro de 2007em um hospital, na Secretaria de Saúde e em um laboratório de análises clinicas, utilizando-se iscas atrativas não tóxicas, as seguintes espécies foram registradas foram registradas: Tapinoma melanocephalum (Fabricius, 1793); Monomorium pharaonis (Linnaeus, 1758); Paratrechina fulva (Mayr, 1862); Pheidole triconstricta Forel, 1886; Pheidole sp.1; Pheidole sp.2; Pheidole sp.3; Pheidole sp.4; Camponotus atriceps (Fr.Smith, 1858); Brachymyrmex sp.; Dorymyrmex sp. Como micro-organismos constatou-se a presença de bactérias: Staphylococcus sp.; Serratia sp.; Klebsiella sp.; Escherichia coli; Salmonella sp., Pseudomonas sp. e de fungos Cladosporium sp.; Fusarium sp.; Trichophyton sp.; Aspergillus sp. e Penicillium sp. além de leveduras.