ABSTRACT
Among the foremost persistent heavy metal ions in the ecosystem, mercury (Hg2+) remains intimidating to the environment by producing a catastrophic effect on the environment as well as on mankind due to the exacerbation of anthropogenic activities. Therefore, it has become necessary to develop superlative techniques for its detection even at low concentrations. The conventional approaches for Hg2+ ions are quite laborious, and expensive, and require expertise in operating sophisticated instruments. To overcome these limitations, aptamer-based biosensors emerged as a promising tool for its detection. DNA-based aptamers have evolved as a significant technique by detecting them even in ppb levels. This review outlines the progress in aptamer-based biosensors from the year 2019-2023 by inducing changes in the electrochemical signal or by fluorescent/colorimetric approaches. The electrochemical sensors used nanomaterial electrodes for increasing the sensitivity whereas fluorescent and colorimetric sensors exhibit quenching or strong fluorescence in the presence of Hg2+ ions depending upon the prevailing mechanism or visible color changes. This perturbation in the signals could be attributed to the formation of the T-Hg2+ -T complex with the aptamers in the presence of ions revealing its real-time and biological applications in living or cancerous cells. Furthermore, next-generation biosensors are suggested to bring a paradigm shift to the integration of high-end smartphones, machine learning, artificial intelligence, etc.
ABSTRACT
In recent years, the field of Supramolecular Chemistry has witnessed tremendous progress owing to the development of versatile optical sensors for the detection of harmful biological analytes. Nitrobenzoxadiazole (NBD) is one such scaffold that has been exploited as fluorescent probes for selective recognition of harmful analytes and their optical imaging in various cell lines including HeLa, PC3, A549, SMMC-7721, MDA-MB-231, HepG2, MFC-7, etc. The NBD-derived molecular probes are majorly synthesized from the chloro derivative of NBD via nucleophilic aromatic substitution. This general NBD moiety ligation method to nucleophiles has been leveraged to develop various derivatives for sensing analytes. NBD-derived probes are extensively used as optical sensors because of remarkable properties like excellent stability, large Stoke's shift, high efficiency and stability, visible excitation, easy use, low cost, and high quantum yield. This article reviewed NBD-based probes for the years 2017-2023 according to the sensing of analyte(s), including cations, anions, thiols, and small molecules like hydrogen sulfide. The sensing mechanism, designing of the probe, plausible binding mechanism, and biological application of chemosensors are summarized. The real-time application of optical sensors has been discussed by various methods, such as paper strips, molecular logic gates, smartphone detection, development of test kits, etc. This article will update the researchers with the in vivo and in vitro biological applicability of NBD-based molecular probes and challenges the research fraternity to design, propose, and develop better chemosensors in the future possessing commercial utility.
