ABSTRACT
While various methods exist for synthesizing silver nanoparticles (AgNPs), green synthesis has emerged as a promising approach due to its affordability, sustainability, and suitability for biomedical purposes. However, green synthesis is time-consuming, necessitating the development of efficient and cost-effective techniques to minimize reaction time. Consequently, researchers have turned their attention to photo-driven processes. In this study, we present the photoinduced bioreduction of silver nitrate (AgNO3) to AgNPs using an aqueous extract of Ulva lactuca, an edible green seaweed. The phytochemicals found in the seaweed functioned as both reducing and capping agents, while light served as a catalyst for biosynthesis. We explored the effects of different light intensities and wavelengths, the initial pH of the reaction mixture, and the exposure time on the biosynthesis of AgNPs. Confirmation of AgNP formation was achieved through the observation of a surface plasmon resonance band at 428 nm using an ultraviolet-visible (UV-vis) spectrophotometer. Fourier transform infrared spectroscopy (FTIR) revealed the presence of algae-derived phytochemicals bound to the outer surface of the synthesized AgNPs. Additionally, high-resolution transmission electron microscopy (HRTEM) and atomic force microscopy (AFM) images demonstrated that the NPs possessed a nearly spherical shape, ranging in size from 5 nm to 40 nm. The crystalline nature of the NPs was confirmed by selected area electron diffraction (SAED) and X-ray diffraction (XRD), with Bragg's diffraction pattern revealing peaks at 2θ = 38°, 44°, 64°, and 77°, corresponding to the planes of silver 111, 200, 220, and 311 in the face-centered cubic crystal lattice of metallic silver. Energy-dispersive X-ray spectroscopy (EDX) results exhibited a prominent peak at 3 keV, indicating an Ag elemental configuration. The highly negative zeta potential values provided further confirmation of the stability of AgNPs. Moreover, the reduction kinetics observed via UV-vis spectrophotometry demonstrated superior photocatalytic activity in the degradation of hazardous pollutant dyes, such as rhodamine B, methylene orange, Congo red, acridine orange, and Coomassie brilliant blue G-250. Consequently, our biosynthesized AgNPs hold great potential for various biomedical redox reaction applications.
ABSTRACT
Plastics play an essential role in food packaging; their primary function is to preserve the nature of the food, ensure adequate shelf life and ensure food safety. Plastics are being produced on a global scale in excess of 320 million tonnes annually, with demand rising to reflect the material in wide range of applications. Nowadays, the packaging industry is a significant consumer of synthetic plastic made from fossil fuels. Petrochemical-based plastics are regarded as the preferred material for packaging. Nonetheless, using these plastics in large quantities results in a long-standing environment. Environmental pollution and the depletion of fossil fuels have prompted researchers and manufacturers to develop eco-friendly biodegradable polymers to replace petrochemical-based polymers. As a result, the production of eco-friendly food packaging material has sparked increased interest as a viable alternative to petrochemical-based polymers. Polylactic acid (PLA) is one of the compostable thermoplastic biopolymers that is biodegradable and renewable in nature. High-molecular-weight PLA can be used to produce fibres, flexible, non-wovens, hard and durable materials (100,000 Da or even higher).The chapter focuses on food packaging techniques, food industry waste, biopolymers, their classification, PLA synthesis, the importance of PLA properties for food packaging, and technologies used to process PLA in food packaging.
Subject(s)
Biodegradable Plastics , Food Packaging , Green Chemistry Technology , Polyesters , Biodegradable Plastics/chemical synthesis , Biodegradable Plastics/chemistry , Biopolymers/chemistry , Fermentation , Food Packaging/methods , Green Chemistry Technology/methods , Polyesters/chemical synthesis , Polyesters/chemistry , Polymerization , Humans , AnimalsABSTRACT
In most developing countries, the availability of drinking water is a major problem. This creates the need for treatment of wastewater, reusability of water, etc. The membrane technology has its place in the market for treating such water. This review compares polymeric membrane fabrication techniques, characteristics, and factors responsible for effective membrane separation for different materials. Although extensive knowledge is available on membrane fabrication, fabricating a membrane is still more challenging, which is more prone to antifouling properties. The competency in different fabrication methods like phase inversion, interfacial polymerization, stretching, track etching and electrospinning are elucidated in the current study. Further, the challenges and adaptability of different application fabrication methods are studied. Important surface parameters like surface wettability, roughness, surface tension, pore size, surface charge, surface functional group and pure water flux are analyzed for different polymeric membranes. In addition, the properties responsible for fouling the membrane are also covered in detail. Flow direction and velocity are the main factors that characterize a membrane's antifouling nature. Antifouling separation can still be achieved by characterizing feed properties such as pH, temperature, diffusivity, ion concentration, and surface content. Understanding fouling properties is a key to progress in membrane technology to develop an effective membrane separation.
Subject(s)
Membranes, Artificial , Water Purification , Polymers/chemistry , Wastewater/chemistry , Water/chemistry , Water Purification/methodsABSTRACT
Contamination of aquatic systems with pharmaceuticals, personal care products, steroid hormones, and agrochemicals has been an immense problem for the earth's ecosystem and health impacts. The environmental issues of well-known persistence pollutants, their metabolites, and other micro-pollutants in diverse aquatic systems around the world were collated and exposed in this review assessment. Waste Water Treatment Plant (WWTP) influents and effluents, as well as industrial, hospital, and residential effluents, include detectable concentrations of known and undiscovered persistence pollutants and metabolites. These components have been found in surface water, groundwater, drinking water, and natural water reservoirs receiving treated and untreated effluents. Several studies have found that these persistence pollutants, and also similar recalcitrant pollutants, are hazardous to a variety of non-targeted creatures in the environment. In human and animals, they can also have severe and persistent harmful consequences. Because these pollutants are harmful to aquatic organisms, microbial degradation of these persistence pollutants had the least efficiency. Fortunately, only a few wild and Genetically Modified (GMOs) microbial species have the ability to degrade these PPCPs contaminants. Hence, researchers have been studying the degradation competence of microbial communities in persistence pollutants of Pharmaceutical and Personal Care Products (PPCPs) and respective metabolites for decades, as well as possible degradation processes in various aquatic systems. As a result, this review provides comprehensive information about environmental issues and the degradation of PPCPs and their metabolites, as well as other micro-pollutants, in aquatic systems.
Subject(s)
Cosmetics , Environmental Pollutants , Water Pollutants, Chemical , Animals , Cosmetics/analysis , Ecosystem , Environmental Monitoring , Pharmaceutical Preparations , Wastewater/chemistry , Water Pollutants, Chemical/analysisABSTRACT
Conversion of CO2 into carbonaceous fuels with the aid of solar energy has been an important research subject for decades. Owing to their excellent electron-accepting capacities, fullerene derivatives have been extensively used as n-type semiconductors. This work reports that the fulleropyrrolidine functionalized with 4,7-di(thiophen-2-yl)benzo[c][1,2,5]thiadiazole, abbreviated as DTBT-C60 , could efficiently catalyze the photoreduction of CO2 to CO. The novel C60 -chromophore dyad structure facilitated better usage of solar light and effective dissociation of excitons. Consequently, the DTBT-C60 exhibited a promising CO yield of 144â µmol gcat -1 under AM1.5G solar illumination for 24â h. Moreover, the isotope experiments demonstrated that water molecules could function as an electron source to reactivate DTBT-C60 . Impressively, DTBT-C60 exhibited an extremely durable catalytic activity for more than one week, facilitating the practical application of photochemical CO2 reaction.
ABSTRACT
A study was carried out on the possibility of orderly and spontaneous dimerization at room temperature of C60 cages in fullerene liquid crystal fullerene dyads (R-C60). For this purpose, dyads with a structural elements feature supporting π-stacking and Van der Waals interactions were tested, due to the presence of terthiophene donors linked through an α-position or dodecyloxy chains. In addition, this possibility was also tested and compared to dyads with shorter substituents and the pristine C60. Research has shown that only in dyads with the features of liquid crystals, π-dimerization of C60 units occurs, which was verified by electrochemical and spectroelectrochemical (ESR) measurements. Cyclic voltammetry and differential voltammetry studies reveal π-dimerization in liquid crystal dyad solution even without the possibility of previous polymerization (cathodic or anodic) under conditions in the absence of irradiation and without the availability of reaction initiators, and even with the use of preliminary homogenization. These dyads undergo six sequential, one-electron reductions of π-dimer (R-C60···C60-R), where two electrons are added successively to each of the two fullerene cages and first form two radical anion system (R-C60)â¢-(R-C60)â¢- without pairing with the characteristics of two doublets. Similarly, the second reductions of π-dimer occur at potentials that are close to the reduction potential for the conversion to a system of two triplet dianions (R-C60)2-(R-C60)2-. Electron paramagnetic resonance spectra indicate a significant interaction between C60 cages. Interestingly, the strength of intermolecular bonds is so significant that it can overcome Coulombic repulsion, even with such highly charged particles as dianions and trianions. Such behavior has been revealed and studied so far only in covalently bonded C60 dimers.
ABSTRACT
Rivers are the most significant natural resources that afford outstanding habitation and nourishment for numerous living organisms. Urbanization and industrialization pollute rivers rendering their water unhealthy for consumption. Hence, this work was designed to find a potential native pollutant removing algae from polluted water. The physicochemical properties of the tested river water such as Electric Conductivity (EC), turbidity, total hardness, Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Ca, SO2-, and NH3, NO3, NO2, PO4, Mg, F- and Cl- contents were not within the permissible limits. Lab-scale and field-based phycoremediation treatments with the indigenous native microalgal species, Chlorella sp. from the Thirumanimutharu river water sample were set up for 15 days with three different (Group I, II, and III) biomass densities (4 × 104, 8 × 104, and 12 × 104 cells mL-1). Group III of both the lab-scale and field based treatments showed the maximum reduction in the physicochemical parameters compared to the other groups. Further, the group III of the field based study showed an extensive reduction in BOD (34.51%), COD (32.53%), NO3, NO2, free NH3 (100%) and increased dissolved oxygen (DO) (88.47%) compared to the lab scale study. In addition, the trace elements were also reduced significantly. The pollutant absorbing active functional moieties (O-H, CO, and CN) found on Chlorella sp. had been confirmed by Fourier-Transform Infrared Spectroscopy (FTIR) analysis. In the Scanning Electron Microscope (SEM) study, significant morphological changes on the surface of the treated Chlorella sp. were noticed compared with the untreated Chlorella sp. biomass, which also confirmed the absorption of the pollutants during treatment.
Subject(s)
Chlorella , Water Pollutants, Chemical , Biological Oxygen Demand Analysis , Environmental Monitoring , Fresh Water , Rivers , Water , Water Pollutants, Chemical/analysis , Water QualityABSTRACT
Cyanobacteria play a vital role in supplying nitrogen into the soil and aquatic ecosystem. It has an extra chromosomal DNA, whose role is not yet defined well. Isolation and characterization of extra chromosomal DNA in cyanobacteria might help to understand its survival mechanism. Cylindrospermum stagnale isolated (and deposited in NRMCF 3001) from soil showed presence of four plasmids namely pCYLM01, pCYLM02, pCYLM03, and pCYLM04. The following plasmids pCYLM01 and pCYLM02 were subjected to restriction digestion using HindIII restriction enzyme and cloned into pBlueScriptSK(-) vector. The sequence of pCYLM01 contained 4 potential open reading frames (ORFs) that have amino acids in the range of 59-299. Among them, ORF1 shows high sequence homology to the bacterial replication initiator family protein as evident from BLASTP analysis. The analysis of 4359 bp plasmid pCYLM02 sequence revealed 7 ORFs which are longer than 50 amino acids in length. The ORF2 of pCYLM02 has 243 amino acids and is represented in the plasmid sequence from 3045 to 3776 bp. The ORF3 of pCYLM02 corresponds to the plasmid sequence from 2323 to 2976 and codes for a putative protein of 217 amino acids long. A number of small ORFs below 50 bp were also found in the sequence analysis.
ABSTRACT
Microalgal isolates (Chlorella sp. and Spirulina sp.) and yeast isolates (Candida albicans and Saccharomyces sp.) were employed as the resources of biodiesel production and silver nanoparticle synthesis. The prominent peaks of the FTIR spectrum accustomed the efficient lipid property. The developed profile containing fatty acid methyl ester (FAME) displayed the elevated amount of both saturated (C15:0, C17:0, C21:0) and unsaturated (C17:1, C18:2, C20:4) fatty acids. The physicochemical properties analyzed by using Biodiesel analyzer V1.1.software, confirmed the competency of the isolates for sustainable biodiesel production. Biosynthesis of silvernanoparticles (AgNPs) were accomplished extracellularly by using supernatant of microalgal and yeast culture. The maximum absorbance at 420 and 421â¯nm under UV-visible spectra showed the presence of nanoparticles. The purity of the synthesized AgNPs were analyzed by XRD analysis. The elemental silver presence was affirmed by EDAX, SEM and AFM, the results revealed spherical crystalline shaped nanoparticles of size ranging from 2.0 to 7.3â¯nm. The antimicrobial efficacy of the silver nanoparticles (AgNPs) against various clinical pathogens which includes Bacillus sp., E. coli, Klebsiella sp., Proteus sp. and Staphylococcus aureus were observed. However, enhanced antimicrobial activity was displayed by the AgNPs, produced by Candida albicans (12â¯mm) against Bacillus sp., and E.coli, the nanoparticle produced by Chlorella sp. showed the least antagonistic activity (07â¯mm).
Subject(s)
Anti-Bacterial Agents/chemistry , Biofuels/analysis , Candida albicans/chemistry , Metal Nanoparticles/chemistry , Microalgae/chemistry , Saccharomyces/chemistry , Silver/chemistry , Anti-Bacterial Agents/pharmacology , Candida albicans/metabolism , Chromatography, Thin Layer , Fatty Acids/chemistry , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Green Chemistry Technology , Metal Nanoparticles/toxicity , Microalgae/metabolism , Microbial Sensitivity Tests , Microscopy, Atomic Force , Saccharomyces/metabolism , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform Infrared , X-Ray DiffractionABSTRACT
Novel two-dimensional conjugated copolymer, abbreviated as PDTBSeVTT-2TF, containing electron-deficient 4,7-di(thiophen-2-yl)benzo[c][1,2,5]selenodiazole (DTBSe) unit, conjugated vinyl-terthiophene (VTT) side chain and 3,3'-difluoro-2,2'-bithiophene (2TF) was designed and synthesized using microwave-assisted Stille cross-coupling polymerization. UVâ»visible absorption and cyclic voltammetry studies revealed that this copolymer possesses a strong and broad absorption in the range of 300â»800 nm and a narrow optical bandgap (Eg) of 1.57 eV with low-lying HOMO and LUMO energy levels. Further, the bulk heterojunction polymer solar cells (PSCs) were fabricated using PDTBSeVTT-2TF as donor and [6,6]-phenyl-C71-butyric acid methyl ester (PC71BM) as acceptor with an inverted device structure of ITO/ZnO/PDTBSeVTT-2TF:PC71BM/V2O5/Ag. The processing temperature of blend solution for preparing PDTBSeVTT-2TF:PC71BM active layer showed obvious impact on the photovoltaic performance of solar devices. The cell fabricated from the blend solution at 65 °C exhibited enhanced power conversion efficiencies (PCE) of 5.11% with a Jsc of 10.99 mA/cm-2 compared with the one at 50 °C, which had a PCE of 4.69% with a Jsc of 10.10 mA/cm-2. This enhancement is due to the dissolution of PDTBSeVTT-2TF clusters into single molecules and small aggregates, improving the miscibility between the polymer and PC71BM and thus increasing the donor/acceptor interface.
ABSTRACT
The cyanobacteria (blue-green algae) are photosynthetic prokaryotes having applications in human health with numerous biological activities and as a dietary supplement. It is used as a food supplement because of its richness in nutrients and digestibility. Many cyanobacteria (Microcystis sp, Anabaena sp, Nostoc sp, Oscillatoria sp., etc.) produce a great variety of secondary metabolites with potent biological activities. Cyanobacteria produce biologically active and chemically diverse compounds belonging to cyclic peptides, lipopeptides, fatty acid amides, alkaloids and saccharides. More than 50% of the marine cyanobacteria are potentially exploitable for extracting bioactive substances which are effective in killing cancer cells by inducing apoptotic death. Their role as anti-viral, anti-tumor, antimicrobial, anti-HIV and a food additive have also been well established. However, such products are at different stages of clinical trials and only a few compounds have reached to the market.
Subject(s)
Biological Factors/pharmacology , Chlorophyta/chemistry , Cyanobacteria/chemistry , Dietary Supplements/analysis , Animals , Biological Factors/metabolism , Clinical Trials as Topic , Cyanobacteria/metabolism , Drug Therapy/trends , HumansABSTRACT
A series of novel p-type conjugated copolymers, PTTVBDT, PTTVBDT-TPD, and PTTVBDT-DPP, cooperating benzo[1,2-b:4,5-b']dithiophene (BDT) and terthiophene-vinylene (TTV) units with/without thieno[3,4-c]pyrrole-4,6-dione (TPD) or pyrrolo[3,4-c]pyrrole-1,4-dione (DPP) via Stille polymerization were synthesized and characterized. Copolymer PTTVBDT shows a low-lying HOMO energy level and ordered molecular-packing behavior. Furthermore, two terpolymers, PTTVBDT-TPD and PTTVBDT-DPP, display stronger absorption ability, alower-lying HOMO energy level, and preferred molecular orientation, due to the replacement TTV-monomer units with electron-deficient groups. Furthermore, bulk-heterojunction organic solar cells were fabricated using blends of the PTTVBDT-TPD, and PC61BM gave the best power conversion efficiency of 5.01% under the illumination of AM 1.5G, 100 mW·cm-2; the short circuit current (Jsc) was 11.65 mA·cm-2 which displayed a 43.8% improvement in comparison with the PTTVBDT/PC61BM device. These results demonstrate a valid strategy combining the two-dimensional molecular structure with random copolymerization strikes promising conjugated polymers to achieve highly efficient organic photovoltaics.
ABSTRACT
The effects of the spatial arrangement of the conjugated side chains of two-dimensional polymers on their optical, electrochemical, molecular-packing, and photovoltaic characteristics were investigated. Accordingly, novel polythiophenes with horizontally (PBTTTV-h) and vertically (PBTTTV-v) grafted terthiophenevinylene (TTV) conjugated side chains were synthesized that display two and one UV-vis peaks, respectively; the difference is due to the different constitutions of the conjugated side-chains. Because the spatial arrangement affects the molecular self-assembly, PBTTTV-h shows stronger crystallinity than PBTTTV-v, which enhances the charge mobility in devices. Moreover, PBTTTV-h has a lower HOMO energy level (−5.49 eV) than PBTTTV-v (−5.40 eV). Bulk heterojunction solar cells fabricated from PBTTTV-h/PC71BM and PBTTTV-v/PC71BM exhibit power conversion efficiencies of 4.75% and 4.00%, respectively, and Voc values of 800 and 730 mV, respectively, under AM1.5G illumination (100 mW cm(−2)). Thus, the architecture of the TTV conjugated side chains affects the optical, electrochemical, and photovoltaic properties; this study provides more ideas for improving 2-D conjugated polymers for semiconductor devices.
ABSTRACT
India is the fifth largest primary energy consumer and fourth largest petroleum consumer after USA, China, and Japan. Despite the global economic crisis, India's economy is expected to grow at 6 to 8 %/year. There is an extreme dependence on petroleum products with considerable risks and environmental issues. Petroleum-derived transport fuels are of limited availability and contribute to global warming, making renewable biofuel as the best alternative. The focus on biogas and biomass-based energy, such as bioethanol and biohydrogen, will enhance cost-effectiveness and provide an opportunity for the rural community. Among all energy sources, microalgae have received, so far, more attention due to their facile adaptability to grow in the photobioreactors or open ponds, high yields, and multiple applications. Microalgae can produce a substantial amount of triacylglycerols as a storage lipid under photooxidative stress or other adverse environmental conditions. In addition to renewable biofuels, they can provide different types of high-value bioproducts added to their advantages, such as higher photosynthetic efficiency, higher biomass production, and faster growth compared to any other energy crops. The viability of first-generation biofuels production is, however, questionable because of the conflict with food supply. In the future, biofuels should ideally create the environmental, economic, and social benefits to the communities and reflect energy efficiency so as to plan a road map for the industry to produce third-generation biofuels.
Subject(s)
Biofuels , Microalgae/metabolism , Renewable Energy , IndiaABSTRACT
Synthesis of novel quinoline based dicationic benzimidazolophanes and imidazolophanes incorporating various spacer units is described. Some of the quinolinophanes 1b, 3a,3b and 4a exhibit good antibacterial activity against most of the human pathogenic bacteria in the tested concentrations as compared to the other cyclophanes as well as the test control, streptomycin.
Subject(s)
Anti-Bacterial Agents/chemical synthesis , Imidazoles/chemistry , Quinolines/chemical synthesis , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Imidazoles/chemical synthesis , Microbial Sensitivity Tests , Quinolines/chemistry , Quinolines/pharmacologyABSTRACT
Although gene expression profile of pollen has been described, there is limited information regarding a particular phase during anther/pollen development. This work characterizes gene expression pattern at desiccation in lily (Lilium longiflorum Thunb. cv Snow Queen) anthers. We have applied a suppression-subtractive hybridization (SSH) strategy, through which 90 clones were identified and sequenced. These clones resulted in the identification of 42 individual cDNAs among which 33 genes were specifically expressed at the desiccation phase of anthers of >150-mm buds. Fourteen cDNAs were chosen for further examination. Six genes were both dehydration- and abscisic acid (ABA)-inducible whereas the other eight genes were apparently dehydration-irrelevant. The group of dehydration- and ABA-induced genes was also induced by desiccation that developmentally occurs in the anther. The application of fluridone has a significant effect of inhibition on mRNA accumulation of these genes in maturing anthers during which desiccation occurs. Pollen germination analysis indicated that, of those dehydration-irrelevant genes, three were ABA-responsive and the other five were not. Thus, three separate signal pathways that function in the activation of late genes at desiccation during anther development are established. The first is the ABA-dependent pathway induced by environmental stress of dehydration. The other two pathways of signaling triggered by developmental cues, through which one is ABA-dependent and another is ABA-independent. The 14 gene proteins showed spatial and temporal expression patterns and may participate in membrane/cell wall synthesis, cytoskeletal organization, signaling, RNA binding, ubiquitin-mediated degradation and transportation during germination and tube growth.
Subject(s)
Desiccation , Gene Expression Regulation, Plant , Lilium/genetics , Plant Proteins/genetics , Abscisic Acid/pharmacology , Flowers/drug effects , Flowers/genetics , Flowers/physiology , Gene Expression Profiling , Gene Expression Regulation, Plant/drug effects , Germination/drug effects , Germination/genetics , Lilium/drug effects , Lilium/physiology , Nucleic Acid Hybridization , Plant Proteins/metabolism , Signal TransductionABSTRACT
The beta-carotene-yielding microalga, Dunaliella salina (Dunal) Teod. maintained in De Walne's medium was harvested and lyophilized. Fibrosarcoma was induced in rats by 20-methylcholanthrene. 0.5 g and 1.0 g of lyophilized D. salina powder was administered to the rats orally through carboxy methyl cellulose. Cisplatin was administered along with vitamin E to compare the protective effect of D. salina against fibrosarcoma. Administration of D. salina decreased the levels of cholesterol and lactate dehydrogenase as well as the activities of catalase, superoxide dismutase, serum aspartate aminotransaminase, serum alanine aminotransferase, when compared to control. A significant reduction in the levels of hepatic and renal RNA and DNA was observed in the sarcoma rats when treated with D. salina powder. Histopathological studies of tumor tissues showed regenerative and regressive changes. beta-carotene globules isolated from the powder of Dunaliella salina confirmed the presence of 9-cis-beta-carotene and all-trans-beta-carotene.
Subject(s)
Chlorophyta/chemistry , Fibrosarcoma/drug therapy , beta Carotene/administration & dosage , Alanine Transaminase/blood , Animals , Aspartate Aminotransferases/blood , Catalase/blood , Cholesterol/blood , DNA, Neoplasm/metabolism , Fibrosarcoma/metabolism , Fibrosarcoma/pathology , Freeze Drying , Male , RNA, Neoplasm/metabolism , Rats , Rats, Wistar , Superoxide Dismutase/blood , beta Carotene/isolation & purificationABSTRACT
The saline pond microalga, Dunaliella salina (Dunal) Teod. maintained in De Walne's (basal) medium under laboratory conditions was confirmed by amplifying the chromosomal DNA of the microalga by PCR with specific primers MA1 and MA2. Seaweed extracts obtained from Sargassum wightii and Ulva lactuca were amended separately at 1.0%, 1.5%, 2.0% and 2.5% levels to the basal medium in order to assess their potential on the growth and concentration of pigments, viz. Chl a, Chl b and beta-carotene of the alga. beta-Carotene was isolated and visible absorption spectrum was taken at 443 and 475 nm confirmed the presence of 9-cis-beta-carotene and all-trans-beta-carotene isomers. Maximum yield, highest division rate (mu) and highest pigment concentrations were observed in the cells grown in 1.5% S. wightii and 2.0% U. lactuca amended medium and these cells were subjected to DAPI staining. The results of epifluorescence microscopy and image analysis revealed a significant enhancement of the cell and nuclear area of the microalgae.