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Asian Pacific Journal of Tropical Biomedicine
; (12): 116-120, 2017.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-950638
ABSTRACT
Objective To standardize an ELISA protocol to quantify total immunoglobulin A (IgA) from different biological samples. Methods Two independent experiments were conducted. In Experiment 1, total IgA levels were quantified from the lachrymal fluid, tracheal swab, and cloacal swab at various time points from Days 30 to 89 in white Leghorn chickens. Experiment 2 was conducted to evaluate the effect of 50 or 500 ppb of aflatoxin B