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1.
Rev Bras Enferm ; 77(2): e20230383, 2024.
Article in English, Portuguese | MEDLINE | ID: mdl-39045979

ABSTRACT

OBJECTIVES: to analyze the use of spirituality/religiosity by oncology nurse residents in caring for patients with cancer. METHODS: a census, descriptive, sectional study, with 46 nurse residents from three public hospitals in Rio de Janeiro. Data collection took place between August 2020 and January 2021, using a sociodemographic questionnaire, including a question about the use of spirituality/ religiosity to deal with work situations. Descriptive analysis was carried out using SPSS software version 22.0. RESULTS: participants stated that they use religiosity/spirituality in work situations related to patients or themselves. In relation to patients, death was the most mentioned situation among professionals, and for themselves, everyday situations and emotional vulnerability were the most mentioned. FINAL CONSIDERATIONS: spirituality and religiosity are dimensions that guide oncology nurse residents' attitudes.


Subject(s)
Oncology Nursing , Spirituality , Humans , Male , Female , Adult , Surveys and Questionnaires , Oncology Nursing/methods , Oncology Nursing/standards , Brazil , Cross-Sectional Studies , Middle Aged , Nursing Care/methods , Nursing Care/psychology , Attitude of Health Personnel , Neoplasms/psychology , Neoplasms/nursing
2.
Andrology ; 2024 Jun 26.
Article in English | MEDLINE | ID: mdl-38923406

ABSTRACT

BACKGROUND: Androgen insensitivity syndrome (AIS) is a common condition among individuals with differences of sexual development (DSD) and results from germline allelic variants in the androgen receptor (AR) gene. Understanding the phenotypic consequences of AR allelic variants that disrupt the activation function 2 (AF2) region is essential to grasping its clinical significance. OBJECTIVES: This study aims to provide insights into the phenotypic characteristics and clinical impact of AR mutations affecting the AF2 region in AIS patients. We achieve this by reviewing reported AR variants in the AF2 region among individuals with AIS, including identifying a new phenotype associated with the c.2138T>C variant (p.Leu713Pro) in the AR gene. MATERIALS AND METHODS: We comprehensively reviewed AR variants within the AF2 region reported in AIS and applied molecular dynamics simulations to assess the impact of the p.Leu713Pro variant on protein dynamics. RESULTS: Our review of reported AR variants in the AF2 region revealed a spectrum of phenotypic outcomes in AIS patients. Molecular dynamics simulations indicated that the p.Leu713Pro variant significantly alters the local dynamics of the AR protein and disrupts the correlation and covariance between variables. DISCUSSION: The diverse phenotypic presentations observed among individuals with AR variants in the AF2 region highlight the complexity of AIS. The altered protein dynamics resulting from the p.Leu713Pro variant further emphasize the importance of the AF2 region in AR function. CONCLUSION: Our study provides valuable insights into AR mutations' phenotypic characteristics and clinical impact on the AF2 region in AIS. Moreover, the disruption of protein dynamics underscores the significance of the AF2 region in AR function and its role in the pathogenesis of AIS.

3.
Front Oncol ; 14: 1304690, 2024.
Article in English | MEDLINE | ID: mdl-38634051

ABSTRACT

The main objective of the National Project for Research and Incidence of Childhood Leukemias is to reduce early mortality rates for these neoplasms in the vulnerable regions of Mexico. This project was conducted in the states of Oaxaca, Puebla, and Tlaxcala. A key strategy of the project is the implementation of an effective roadmap to ensure that leukemia patients are the target of maximum benefit of interdisciplinary collaboration between researchers, clinicians, surveyors, and laboratories. This strategy guarantees the comprehensive management of diagnosis and follow-up samples of pediatric patients with leukemia, centralizing, managing, and analyzing the information collected. Additionally, it allows for a precise diagnosis and monitoring of the disease through immunophenotype and measurable residual disease (MRD) studies, enhancing research and supporting informed clinical decisions for the first time in these regions through a population-based study. This initiative has significantly improved the diagnostic capacity of leukemia in girls, boys, and adolescents in the regions of Oaxaca, Puebla, and Tlaxcala, providing comprehensive, high-quality care with full coverage in the region. Likewise, it has strengthened collaboration between health institutions, researchers, and professionals in the sector, which contributes to reducing the impact of the disease on the community.

4.
Nursing (Ed. bras., Impr.) ; 27(307): 10068-10073, jan.2024. tab
Article in English, Portuguese | LILACS, BDENF - Nursing | ID: biblio-1531311

ABSTRACT

Objetiva-se analisar as produções cientificas sobre a atenção integral da saúde do homem na Revista Nursing brasileira, São Paulo. Método: trata-se de uma revisão integrativa de literatura, utilizando as bases de dados LILACS e BDENF, o corte epistemológico foi de 2009 a 2019, a amostra final constituiu-se de 10 artigos. Resultados: os dados analisados foram divididos a partir de associações temáticas, em quatro categorias: acesso e acolhimento; saúde sexual e saúde reprodutiva; doenças prevalentes na população masculina e prevenção de violência e acidentes. Conclui-se que o cuidado integral a saúde do homem, e colocá-los como sujeito do cuidado, com autonomia, independência e responsabilidade social.(AU)


The objective is to analyze scientific productions on comprehensive men's health care in the Brazilian Nursing Journal, São Paulo. Method: This is an integrative literature review, using the LILACS and BDENF databases, the epistemological cut was from 2009 to 2019, the final sample consisted of 10 articles. Results: The data analyzed was divided into four categories based on thematic associations: access and reception; sexual health and reproductive health; prevalent diseases in the male population; and prevention of violence and accidents. The conclusion is that comprehensive care for men's health should place them as the subject of care, with autonomy, independence and social responsibility.(AU)


El objetivo es analizar la producción científica sobre atención integral a la salud masculina en la Revista Brasileña de Enfermería, São Paulo. Método: Se trata de una revisión bibliográfica integradora, utilizando las bases de datos LILACS y BDENF, el corte epistemológico fue de 2009 a 2019, la muestra final constó de 10 artículos. Resultados: Los datos analizados se dividieron en cuatro categorías a partir de asociaciones temáticas: acceso y acogida; salud sexual y salud reproductiva; enfermedades prevalentes en la población masculina; y prevención de violencia y accidentes. Se concluye que la atención integral a la salud del hombre debe colocarlo como sujeto de cuidados, con autonomía, independencia y responsabilidad social.(AU)


Subject(s)
Publications for Science Diffusion , Men's Health , Nursing Care
5.
Rev. bras. enferm ; Rev. bras. enferm;77(2): e20230383, 2024. tab
Article in English | LILACS-Express | LILACS, BDENF - Nursing | ID: biblio-1565284

ABSTRACT

ABSTRACT Objectives: to analyze the use of spirituality/religiosity by oncology nurse residents in caring for patients with cancer. Methods: a census, descriptive, sectional study, with 46 nurse residents from three public hospitals in Rio de Janeiro. Data collection took place between August 2020 and January 2021, using a sociodemographic questionnaire, including a question about the use of spirituality/ religiosity to deal with work situations. Descriptive analysis was carried out using SPSS software version 22.0. Results: participants stated that they use religiosity/spirituality in work situations related to patients or themselves. In relation to patients, death was the most mentioned situation among professionals, and for themselves, everyday situations and emotional vulnerability were the most mentioned. Final Considerations: spirituality and religiosity are dimensions that guide oncology nurse residents' attitudes.


RESUMEN Objetivos: analizar el uso de la espiritualidad/religiosidad por parte de enfermeras residentes de oncología en el cuidado del paciente con cáncer. Métodos: estudio censal, descriptivo, seccional, con 46 enfermeros residentes de tres hospitales públicos de Río de Janeiro. La recolección de datos se realizó entre agosto de 2020 y enero de 2021, mediante un cuestionario sociodemográfico, que incluía una pregunta sobre el uso de la espiritualidad/religiosidad para afrontar situaciones laborales. El análisis descriptivo se realizó mediante el software SPSS, versión 22.0. Resultados: los participantes afirmaron utilizar la religiosidad/espiritualidad en situaciones laborales relacionadas con el cliente o con ellos mismos. En relación al cliente, la muerte fue la situación más mencionada entre los profesionales, y para usted, las situaciones cotidianas y su vulnerabilidad emocional fueron las más mencionadas. Consideraciones Finales: espiritualidad y religiosidad son dimensiones que orientan las actitudes de los enfermeros residentes en oncología.


RESUMO Objetivos: analisar o uso da espiritualidade/religiosidade por enfermeiros residentes em oncologia na assistência ao paciente oncológico. Métodos: estudo censitário, descritivo, do tipo seccional, com 46 enfermeiros residentes de três hospitais públicos do Rio de Janeiro. A coleta de dados ocorreu entre agosto de 2020 e janeiro de 2021, utilizando um questionário sociodemográfico, incluindo uma pergunta acerca do uso da espiritualidade/religiosidade para lidar com situações do trabalho. A análise descritiva foi realizada por meio do software SPSS, versão 22.0. Resultados: os participantes afirmaram fazer uso da religiosidade/espiritualidade em situações de trabalho relacionadas ao cliente ou a si próprio. Em relação ao cliente, a morte foi a situação mais referida entre os profissionais, e para si, as situações do dia a dia e sua vulnerabilidade emocional foram as mais referidas. Considerações Finais: a espiritualidade e a religiosidade são dimensões que guiam as atitudes dos enfermeiros residentes em oncologia.

6.
Mem Inst Oswaldo Cruz ; 118: e220259, 2023.
Article in English | MEDLINE | ID: mdl-37531506

ABSTRACT

BACKGROUND: Chikungunya is a mosquito-borne virus that has been causing large outbreaks in the Americas since 2014. In Brazil, Asian-Caribbean (AC) and East-Central-South-African (ECSA) genotypes have been detected and lead to large outbreaks in several Brazilian states. In Rio Grande do Sul (RS), the southernmost state of Brazil, the first cases were reported in 2016. OBJECTIVES AND METHODS: We employed genome sequencing and epidemiological investigation to characterise the Chikungunya fever (CHIKF) burden in RS between 2017-2021. FINDINGS: We detected an increasing CHIKF burden linked to travel associated introductions and communitary transmission of distinct lineages of the ECSA genotype during this period. MAIN CONCLUSIONS: Until 2020, CHIKV introductions were most travel associated and transmission was limited. Then, in 2021, the largest outbreak occurred in the state associated with the introduction of a new ECSA lineage. CHIKV outbreaks are likely to occur in the near future due to abundant competent vectors and a susceptible population, exposing more than 11 million inhabitants to an increasing infection risk.


Subject(s)
Chikungunya Fever , Chikungunya virus , Animals , Humans , Chikungunya virus/genetics , Brazil/epidemiology , Travel , Phylogeny , Mosquito Vectors , Disease Outbreaks , Genotype
7.
J Clin Endocrinol Metab ; 109(1): 68-79, 2023 Dec 21.
Article in English | MEDLINE | ID: mdl-37572362

ABSTRACT

CONTEXT: Despite high abundance of small indels in human genomes, their precise roles and underlying mechanisms of mutagenesis in Mendelian disorders require further investigation. OBJECTIVE: To profile the distribution, functional implications, and mechanisms of small indels in the androgen receptor (AR) gene in individuals with androgen insensitivity syndrome (AIS). METHODS: We conducted a systematic review of previously reported indels within the coding region of the AR gene, including 3 novel indels. Distribution throughout the AR coding region was examined and compared with genomic population data. Additionally, we assessed their impact on the AIS phenotype and investigated potential mechanisms driving their occurrence. RESULTS: A total of 82 indels in AIS were included. Notably, all frameshift indels exhibited complete AIS. The distribution of indels across the AR gene showed a predominance in the N-terminal domain, most leading to frameshift mutations. Small deletions accounted for 59.7%. Most indels occurred in nonrepetitive sequences, with 15.8% situated within triplet regions. Gene burden analysis demonstrated significant enrichment of frameshift indels in AIS compared with controls (P < .00001), and deletions were overrepresented in AIS (P < .00001). CONCLUSION: Our findings underscore a robust genotype-phenotype relationship regarding small indels in the AR gene in AIS, with a vast majority presenting complete AIS. Triplet regions and homopolymeric runs emerged as prone loci for small indels within the AR. Most were frameshift indels, with polymerase slippage potentially explaining half of AR indel occurrences. Complex frameshift indels exhibited association with palindromic runs. These discoveries advance understanding of the genetic basis of AIS and shed light on potential mechanisms underlying pathogenic small indel events.


Subject(s)
Androgen-Insensitivity Syndrome , Receptors, Androgen , Humans , Male , Androgen-Insensitivity Syndrome/genetics , Genome, Human , Mutagenesis , Mutation , Phenotype , Receptors, Androgen/genetics
8.
Enferm. foco (Brasília) ; 14: 1-7, mar. 20, 2023. tab
Article in Portuguese | LILACS, BDENF - Nursing | ID: biblio-1433852

ABSTRACT

Objetivo: Caracterizar os estudos de intervenção cadastrados por enfermeiros no Registro Brasileiro de Ensaios Clínicos. Métodos: Estudo descritivo, com abordagem quantitativa, realizado mediante acesso virtual ao Registro Brasileiro de Ensaios Clínicos, para busca dos estudos cadastrados na plataforma. Após leitura dos 3350 resumos disponíveis, os 320 cadastrados por enfermeiros foram selecionados para compor a amostra. Foi realizada a análise descritiva, no software R. Resultados: Foram encontrados estudos registrados entre 2010 e 2019, dos quais 49,7% localizavam-se na região Sudeste, 49,3% cadastrados por doutores, 46,3% filiados a universidades federais. A área da enfermagem mais estudada (18,8%) tratou-se de saúde do adulto, 72,9% possuíam dois braços, 53,1% com recrutamento concluído, 50,3% realizados com cegamento e o tipo de intervenções mais estudado (21,9%) foi a educativa. Conclusão: A maioria dos estudos de intervenção cadastrados por enfermeiros no Registro Brasileiro de Ensaios Clínicos testaram intervenções educativas, em adultos, possuíram cegamento e foram randomizados. (AU)


Objective: To characterize the intervention studies registered by nurses in the Brazilian Registry of Clinical Trials. Methods: Descriptive study, with quantitative approach, carried out through virtual access to the Brazilian Registry of Clinical Trials, to search for the studies registered on the platform. After reading the 3350 abstracts available, the 320 registered by nurses were selected to make up the sample. Descriptive analysis was performed in the software R. Results: Studies registered between 2010 and 2019 were found, of which 49.7% were in the Southeast region, 49.3% registered by doctors, 46.3% affiliated to federal universities. The most studied nursing area (18.8%) it was adult health, 72.9% had two arms, 53.1% with completed recruitment, 50.3% performed blinding and the most studied type of interventions (21.9%) was the educational. Conclusion: Most intervention studies registered by nurses in the Brazilian Registry of Clinical Trials tested educational interventions in adults, blinded and randomized. (AU)


Objetivo: Caracterizar los estudios de intervención registrados por enfermeros en el Registro Brasileño de Ensayos Clínicos. Métodos: Estudio descriptivo, con enfoque cuantitativo, realizado a través del acceso virtual al Registro Brasileño de Ensayos Clínicos, para buscar estudios registrados en la plataforma. Después de leer los 3350 resúmenes disponibles, los 320 registrados por enfermeros fueron seleccionados para componer la muestra. Se realizó análisis descriptivo en el software R. Resultados: Se encontraron estudios registrados entre 2010 y 2019, de los cuales el 49,7% se ubicaron en la región Sudeste, el 49,3% fueron registrados por médicos, el 46,3% fueron afiliados a universidades federales. El área de enfermería más estudiada (18,8 %) fue la salud del adulto, el 72,9 % tenía dos brazos, el 53,1 % había completado el reclutamiento, el 50,3 % estaba cegada y el tipo de intervenciones más estudiadas (21,9 %) fue la educativa. Conclusión: La mayoría de los estudios de intervención registrados por enfermeros en el Registro Brasileño de Ensayos Clínicos probaron intervenciones educativas en adultos, fueron ciegos y aleatorizados. (AU)


Subject(s)
Clinical Trial , Nursing Research , Nursing , Evidence-Based Practice
9.
Int J Afr Nurs Sci ; 18: 100518, 2023.
Article in English | MEDLINE | ID: mdl-36530550

ABSTRACT

Introduction: The treatment of COVID-19 is still challenge. So convalescent plasma can be an important alternative of treatment. Protocols with nursing care during infusion is very important to guide an effective and safety care. Objective: to analyze the evidence in the literature on the action of convalescent plasma, of the use of protocols with nursing care to use convalescent plasma and build a nursing care protocol for transfusion in patients with COVID-19. Methods: Methodological study carried out in two stages: scoping review. The search was done using the descriptors: convalescent plasma transfusion, convalescent plasma, and acute respiratory syndromes or COVID-19, to found protocols and effectiveness of convalescent plasm. Beside was done a specialist panel to build the protocol. Results: Low-evidence studies have shown improvement in the clinical signs of COVID-19 using Convalescent Plasma, reduction or elimination of viral load, benefits in the production of lymphocytes, decreases C-reactive protein, increases titers of anti-SARS-CoV-2 antibodies, positive evolution in lung involvement identified by X-rays, decrease in hospitalization. No studies were found in the databases on the protocol for clinical nursing care in plasma transfusion. Therefore, a protocol was developed with the description of clinical nursing care to be performed before, during and after the transfusion by plasma: checking of vital signs and indicative signs of transfusion reaction, measurement of oxygen saturation, assessment of venous access and checking of the level of consciousness. Conclusion: There are no evidence studies to support the use of plasma, nor anything related to bundles.

10.
Mem. Inst. Oswaldo Cruz ; 118: e220259, 2023. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1448699

ABSTRACT

BACKGROUND Chikungunya is a mosquito-borne virus that has been causing large outbreaks in the Americas since 2014. In Brazil, Asian-Caribbean (AC) and East-Central-South-African (ECSA) genotypes have been detected and lead to large outbreaks in several Brazilian states. In Rio Grande do Sul (RS), the southernmost state of Brazil, the first cases were reported in 2016. OBJECTIVES AND METHODS We employed genome sequencing and epidemiological investigation to characterise the Chikungunya fever (CHIKF) burden in RS between 2017-2021. FINDINGS We detected an increasing CHIKF burden linked to travel associated introductions and communitary transmission of distinct lineages of the ECSA genotype during this period. MAIN CONCLUSIONS Until 2020, CHIKV introductions were most travel associated and transmission was limited. Then, in 2021, the largest outbreak occurred in the state associated with the introduction of a new ECSA lineage. CHIKV outbreaks are likely to occur in the near future due to abundant competent vectors and a susceptible population, exposing more than 11 million inhabitants to an increasing infection risk.

11.
Rev. Bras. Cancerol. (Online) ; 69(4)out-dez. 2023.
Article in Portuguese | Sec. Est. Saúde SP, LILACS | ID: biblio-1526068

ABSTRACT

Introdução: O novo coronavírus SARS-CoV-2, causa principal da covid-19, foi identificado em dezembro de 2019 em Wuhan, na China. Desde então, espalhou-se pelo mundo e infectou milhares de pessoas. Serviços de saúde ficaram sobrecarregados com o atendimento de pacientes acometidos pela infecção. A enfermagem neste contexto é a categoria profissional que permanece mais tempo assistindo ao paciente. Diante desses fatos, é importante apresentar as opiniões, vivências e percepções dos enfermeiros atuantes na pandemia. Objetivo: Identificar, na perspectiva de enfermeiros, os impactos da covid-19 em seu cotidiano de trabalho no cuidado às pessoas diagnosticadas com câncer. Método: Estudo descritivo qualitativo baseado na teoria das representações sociais. O cenário do estudo ocorreu em uma instituição pública federal, referência no atendimento ao câncer, com 25 enfermeiros. Os dados foram coletados por meio da técnica de entrevista semiestruturada e de um questionário sociodemográfico. A análise dos dados foi realizada pela técnica de análise de conteúdo temática. Resultados: Participaram da pesquisa 22 enfermeiras (88%) com atuação na área oncológica de oito a 30 anos. A análise resultou em quatro categorias centrais: conceitos atribuídos pelos enfermeiros à covid-19; impactos da pandemia no cotidiano dos profissionais; impactos da pandemia na instituição; e impactos da pandemia em pessoas com câncer. Conclusão: Diante de um evento pandêmico, os enfermeiros foram essenciais para o enfrentamento da covid-19. Percebeu-se que as mudanças na rotina da instituição, o uso de equipamentos de proteção individual (EPI), a redução do número de leitos e o índice de absenteísmo afetaram tanto a vida do profissional quanto a do paciente


Introduction: The new coronavirus SARS-CoV-2 the main cause of the COVID-19, was identified in December 2019 in Wuhan, China. Since then the virus has spread around the world and infected millions of people. Healthcare services have become overwhelmed with the care of patients affected by the infection. Nursing is the health professional who stays longer with the patients. Given these facts, it is essential to know the opinions, experiences, knowledge and perception of nurses while providing care to patients during the pandemic. Objective: Identify, from the perspective of nurses, the impacts of COVID-19 on their daily work in caring for individuals diagnosed with cancer. Method: Descriptive, qualitative approach study based on the theory of social representation. The study scenario was a reference federal public institution in cancer care with 25 nurses. Data were collected through a semi-structured interview technique and a sociodemographic questionnaire. Data analysis was performed using the thematic content analysis technique. Results: The participants were 22 female nurses (88%), with between eight and 30 years of experience in oncology. The analysis resulted in 4 core categories: concepts attributed by nurses to COVID-19; impacts of the pandemic on the daily lives of professionals, impacts of the pandemic on the institution and impacts of the pandemic on oncology patients. Conclusion: Even in a pandemic scenario, the nurses were essential to deal with COVID-19. Changes of the institution routine, the use of personal protective equipment (PPE), reduction of beds and absenteeism affected the lives of professionals and patients


Introducción: El nuevo coronavirus SARS-CoV-2, causa principal del COVID-19, fue identificado en diciembre de 2019 en Wuhan, China. Desde entonces, el virus se ha propagado por todo el mundo y ha infectado a millones de personas. Los servicios sanitarios fueron desbordados por la atención a los pacientes afectados por la infección. La enfermería es la clase profesional sanitaria que permanece más tiempo con los pacientes. Ante estos hechos, es fundamental presentar las opiniones, experiencias, conocimientos y percepción de los enfermeros durante la asistencia a los pacientes durante la pandemia. Objetivo: Identificar, desde la perspectiva de los enfermeros, los impactos del COVID-19 en su trabajo diario en el cuidado de personas diagnosticadas con cáncer. Método: Estudio descriptivo con enfoque cualitativo basado en la teoría de la representación social. El escenario de estudio fue en una institución pública federal de referencia en atención al cáncer, con 25 enfermeros. Los datos fueron recolectados a través de la técnica de entrevista semiestructurada y un cuestionario sociodemográfico. El análisis de los datos se realizó mediante la técnica de análisis de contenido temático. Resultados: Participaron del estudio 22 enfermeras (88%), con experiencia en el campo de la oncología entre ocho y 30 años. El análisis resultó en cuatro categorías centrales: conceptos atribuidos por los enfermeros al COVID-19; impactos de la pandemia en la vida diaria de los profesionales, impactos de la pandemia en la institución e impactos de la pandemia en los pacientes de oncología. Conclusión: Con este estudio se pudo comprobar que incluso ante un evento pandémico, los enfermeros fueron profesionales esenciales para enfrentar el COVID-19. Se percibió que los cambios en la rutina de la institución, el uso de equipos de protección personal (EPP), la reducción del número de camas y el índice de ausentismo, afectaron tanto la vida del profesional como la del paciente


Subject(s)
Social Perception , Coronavirus Infections , Social Representation , Medical Oncology , Nursing, Team
12.
Rev. Bras. Cancerol. (Online) ; 68(3)Jul-Set. 2022.
Article in Portuguese | LILACS, Coleciona SUS | ID: biblio-1398723

ABSTRACT

Introdução: O diagnóstico de câncer atinge diversos aspectos, como emocionais, de relacionamento, espirituais e financeiros, tornando-o um importante problema de saúde pública mundialmente. Objetivo: Analisar a estrutura das representações sociais do câncer para pacientes oncológicos hospitalizados adultos e apontar sua relação com aspectos do cotidiano de enfrentamento do diagnóstico e do adoecimento por essa patologia. Método: De natureza qualitativa, descritiva e exploratória, embasado na Teoria das Representações Sociais. O cenário foi um hospital federal de câncer, no município do Rio de Janeiro, com 111 participantes vivendo o adoecimento oncológico. Foram coletadas as evocações livres ao termo indutor "câncer" e aplicado o questionário para caracterização dos pacientes. A análise se deu por intermédio do software EVOC 2005. Resultados: Participantes, majoritariamente homens (69,3%), com ensino fundamental (57,6%). Apresentaram-se como provável núcleo central: doença, tristeza e morte. O conhecimento reificado do câncer ocorreu por meio da palavra "doençaË®. Reconhece-se o predomínio de elementos negativos, ainda que hajam alguns positivos como tratamento, cura e Deus na representação do grupo. A doença em voga carreia estigma, tornando o enfrentamento um processo doloroso e triste. Conclusão: É possível observar o pesar de estar com câncer na visão dos pacientes, ao passo que a esperança existe e os move em direção à cura. Identifica-se a necessidade de o profissional enfermeiro dar enfoque no que diz respeito à dimensão espiritual e religiosa em seu cuidado, entendendo o indivíduo como um ser único e complexo que demanda cuidados individuais.


Introduction: The diagnosis of cancer affects several aspects, whether emotional, relationships, spiritual and financial, making it an important public health problem worldwide. Objective: To analyze the structure of social representations of cancer for adult hospitalized cancer patients and reveal its relations with daily aspects of coping with the diagnosis and sickening by this pathology. Method: Qualitative, descriptive and exploratory based in the Theory of Social Representations. The scenario was a federal cancer hospital in the city of Rio de Janeiro, with 111 participants living the oncological sickening. Free evocations related to the inducing term "cancer" were collected and a questionnaire was applied to characterize the patients. The EVOC 2005 software was utilized for the analyses. Results: Mostly male participants (69.3%), with elementary education (57.6%) appeared as probable core nucleus: disease, sadness and death. The reified knowledge of cancer is given through the word "diseaseË®. The predominance of negative elements is recognized, although there are some positive ones such as treatment, cure and God in the representation of the group. The current disease carries stigma, making coping a painful and sad process. Conclusion: It is possible to detect the regret the patients express for being with cancer, while hope exists and moves them towards the cure. It is identified the need for the nurse to focus on the spiritual and religious dimensions during the caring process, understanding the individual as a unique and complex being that demands centered care


Introducción: El diagnóstico de cáncer incide en diversos aspectos, ya sean emocionales, relacionales, espirituales y económicos, convirtiéndolo en un importante problema de salud pública a nivel mundial. Objetivo: Analizar la estructura de las representaciones sociales del cáncer en pacientes adultos con cáncer hospitalizados y señalar su relación con aspectos cotidianos del afrontamiento del diagnóstico y la enfermedad por esta patología. Método: Cualitativo, descriptivo y exploratorio, basado en la Teoría de las Representaciones Sociales. El escenario fue un hospital federal de cáncer, en la ciudad de Río de Janeiro, 111 participantes viviendo enfermarse de cáncer. Se recogieron evocaciones gratuitas del término inductor "cáncer" y se aplicó un cuestionario para caracterizar a los pacientes. El análisis se realizó mediante el software EVOC 2005. Resultados: Mayoritariamente varones (69,3%), con educación básica (57,6%). Aparecen como un probable núcleo central: enfermedad, tristeza y muerte. El conocimiento cosificado del cáncer se da a través de la palabra "enfermedadË®. Se reconoce el predominio de elementos negativos, aunque existen algunos positivos como el tratamiento, la cura y Dios en la representación del grupo. La enfermedad actual conlleva un estigma, lo que hace que el afrontamiento sea un proceso doloroso y triste. Conclusión: Es posible observar el arrepentimiento de estar con cáncer en la mirada de los pacientes, mientras existe la esperanza y los mueve hacia la cura. Identifica la necesidad del profesional de enfermería de centrarse en la dimensión espiritual y religiosa en su cuidado, entendiendo al individuo como un ser único y complejo que demanda cuidados individuales


Subject(s)
Humans , Male , Female , Oncology Nursing , Psychology, Social , Adaptation, Psychological , Patient Care , Neoplasms
13.
Rev. epidemiol. controle infecç ; 11(1): 65-68, jan.-mar. 2021. ilus
Article in English | LILACS | ID: biblio-1362431

ABSTRACT

Since its detection in December of 2020, the SARS-CoV2 lineage P.1, descendent of B.1.1.28 lineage, has been identified in several places in Brazil and abroad. This Variant of Concern was considered highly prevalent in Northern Brazil and now is rapidly widening its geographical range. Here, we present epidemiological and genomic information of the first case of P1 lineage in Rio Grande do Sul state, in a patient without reported travel history and a tracked transmission chain. These findings occurred in a tourist destination representing an important hub receiving tourists from diverse places.(AU)


Desde a sua detecção em dezembro de 2020, a linhagem P.1 do SARS-CoV2, descendente da linhagem B.1.1.28, foi identificada em diversos locais no Brasil e no mundo. Essa variante de preocupação era considerada altamente frequente no Norte do Brasil e agora está ampliando rapidamente sua distribuição geográfica. Aqui, apresentamos informações epidemiológicas e genômicas do primeiro caso da linhagem P.1 no Rio Grande do Sul em um paciente sem histórico de viagens relatado e com cadeia de transmissão identificada. Esses achados ocorreram em um destino turístico que representa um importante pólo de recepção de turistas de diversas localidades.(AU)


Desde su detección en diciembre de 2020, del linaje P.1 del SARS-CoV2, derivada de la B.1.1.28, hay sido ampliamente identificada en Brasil y en todo el mundo. Esta variante preocupante es muy frecuente en el norte de Brasil y ahora está ampliando rápidamente su distribución geográfica. Aquí, presentamos información epidemiológica y genómica del primer caso de P.1 en Rio Grande do Sul en un paciente sin antecedentes de viaje y con una cadena de transmisión identificada. Estos datos se han obtenido en un destino turístico que representa un importante centro de acogida de turistas de diferentes lugares.(AU)


Subject(s)
COVID-19/transmission , COVID-19/epidemiology
14.
Rev. Enferm. UERJ (Online) ; 28: e51476, jan.-dez. 2020.
Article in English, Portuguese | BDENF - Nursing, LILACS | ID: biblio-1119621

ABSTRACT

Objetivo: desenvolver um modelo de máscara de tecido, com aplicação do elemento filtrante em celulose, para fins de utilização como barreira física segura para aerossóis, como estratégia de resposta emergencial à pandemia provocada pelo SARS-CoV-2. Método: pesquisa laboratorial realizada por meio de protótipos, testagens empíricas e análises e discussões junto a expertises. Resultados: a condução da pesquisa demonstrou que os aerossóis são retidos pela barreira física de celulose introduzida à estrutura das máscaras, o que motivou a segunda fase do estudo em unidade da Rede Brasileira de Laboratórios Analíticos de Saúde sobre a eficácia desses materiais. Conclusão: a confecção de máscaras de tecido é um fenômeno mundial importante e urgente frente à pandemia da COVID-19. Em função da crise de abastecimento e dos parâmetros ressaltados neste estudo, acredita-se que o uso desse equipamento possa ser estendido a setores não críticos de unidades de saúde, além da população em geral.


Objective: to develop a model of fabric mask, with the application of a cellulose filter element, for use as a safe physical barrier for aerosols, as an emergency response strategy for the SARS-CoV-2 pandemic. Method: laboratory research carried out by means of prototypes, empirical tests and analyses, and discussions with experts. Results: the research demonstrated that aerosols are retained by the physical cellulose barrier introduced into the structure of the masks, which motivated the second phase of the study into the effectiveness of these materials at a unit of the Brazilian Analytical Health Laboratories Network. Conclusion: the making of fabric masks is an important and urgent worldwide phenomenon in tackling the COVID-19 pandemic. In view of the supply crisis and the parameters highlighted in this study, it is believed that the use of this equipment can be extended to non-critical sectors of health units, as well as to the general population.


Objetivo: desarrollar un modelo de mascarilla de tela, con la aplicación de un elemento filtrante de celulosa, para su uso como barrera física segura para aerosoles, como estrategia de respuesta de emergencia para la pandemia SARS-CoV-2. Método: investigación de laboratorio realizada mediante prototipos, pruebas y análisis empíricos y discusiones con expertos. Resultados: la investigación demostró que los aerosoles son retenidos por la barrera física de celulosa introducida en la estructura de las máscaras, lo que motivó la segunda fase del estudio sobre la efectividad de estos materiales en una unidad de la Red Brasileña de Laboratorios Analíticos de Salud. Conclusión: la fabricación de máscaras de tela es un fenómeno mundial importante y urgente para hacer frente a la pandemia de COVID-19. Ante la crisis de oferta y los parámetros resaltados en este estudio, se cree que el uso de este equipamiento puede extenderse a sectores no críticos de las unidades de salud, así como a la población en general.


Subject(s)
Humans , Coronavirus Infections/prevention & control , Personal Protective Equipment/standards , Betacoronavirus , Masks/standards , Materials Testing , Brazil , Cellulose , Filters , Surge Capacity , Pandemics/prevention & control
15.
Texto & contexto enferm ; 29: e20190006, Jan.-Dec. 2020. tab
Article in English | BDENF - Nursing, LILACS | ID: biblio-1145143

ABSTRACT

ABSTRACT Objective: to analyze the sexual practices adopted by university students for the prevention of Sexually Transmitted Infections Method: a quantitative, descriptive and cross-sectional study, conducted in 2016, at a private university in the municipality of Rio de Janeiro, Brazil. A convenience sample was selected, stratified by gender, of 768 students who answered a self-administered questionnaire, structured with variables of sociodemographic characterization and related to knowledge about sexually transmitted infections, sexual practices, prevention practices and care with sexual health. In the analysis, descriptive statistics, chi-square tests and analysis of variance were used, with a significance level of 5%. Results: most of the university students ‒ 654 (85.16%) ‒ had an active sex life and 480 (62.54%) did not use condoms in all their sexual encounters. Among the participants, 509 (84.83%) said they had sexual intercourse with a steady partner, of which 224 (44.01%) used a condom. In the investigated group, 313 (47.86%) had relationships with casual partners, with 199 (63.58%) reporting having used a condom. Among the participants, 174 (26.61%) had their sexual practices classified as adequate/satisfactory. Conclusion: the findings show that the university students investigated present a risk behavior for Sexually Transmitted Infections due to inadequate/unsatisfactory sexual practices. Health education actions should consider cultural and individual aspects of the group in order to encourage reflection on practices for the prevention of sexually transmitted diseases.


RESUMEN Objetivo: analizar las prácticas sexuales adoptadas por estudiantes universitarios para prevenir Infecciones de Transmisión Sexual. Método: estudio cuantitativo, descriptivo y transversal, realizado en el año 2016 en una universidad privada del municipio de Río de Janeiro, Brasil. Ajustada por conveniencia y estratificada por sexo, la muestra estuvo compuesta por 768 estudiantes que respondieron un cuestionario autoaplicado, estructurado con variables de caracterización sociodemográfica y relacionadas con el conocimiento sobre infecciones de transmisión sexual, prácticas sexuales, prácticas de prevención y cuidados con la salud sexual. En el análisis se empleó la estadística descriptiva, pruebas de Chi-cuadrado y análisis de variancia, con un nivel de significancia del 5%. Resultados: la mayoría de los universitarios ‒ 654 (85,16%) ‒ tenía una vida sexual activa y 480 (62,54%) no usaban preservativo en todos sus encuentros sexuales. Entre los participantes, 509 (84,83%) afirmaron tener relaciones sexuales con una pareja fija y, de ellos, 224 (44,01%) utilizaban preservativo. En el grupo investigado, 313 (47,86%) tuvieron relaciones con parejas casuales, y 199 (63,58%) declararon haber usado preservativo. Entre los participantes, las prácticas sexuales de 174 (26,61%) de ellos se clasificaron como adecuadas/satisfactorias. Conclusión: las conclusiones del estudio evidencian que los universitarios investigados presentan un comportamiento de riesgo para contraer Infecciones de Transmisión Sexual como resultado de prácticas sexuales inadecuadas/insatisfactorias. Las medidas de educación en salud deben considerar aspectos culturales e individuales del grupo para favorecer la reflexión sobre las prácticas de prevención de enfermedades de transmisión sexual.


RESUMO Objetivo: analisar as práticas sexuais adotadas por estudantes universitários para prevenção de Infecções Sexualmente Transmissíveis Método: estudo quantitativo, descritivo e transversal, realizado em 2016, em uma universidade privada no município do Rio de Janeiro, Brasil. Selecionou-se amostra por conveniência, estratificada por sexo, de 768 estudantes que responderam a um questionário autoaplicado, estruturado com variáveis de caracterização sociodemográfica e relacionadas ao conhecimento sobre infecções sexualmente transmissíveis, práticas sexuais, práticas de prevenção e cuidados com a saúde sexual. Na análise, empregou-se a estatística descritiva, testes de quiquadrado e análise de variância, com nível de significância de 5%. Resultados: a maioria dos universitários ‒ 654(85,16%) ‒ tinha vida sexual ativa e 480(62,54%) não fazia uso do preservativo em todos os intercursos sexuais. Entre os participantes, 509(84,83%) afirmaram ter relação sexual com parceiro fixo, dos quais 224(44,01%) utilizaram o preservativo. No grupo investigado, 313(47,86%) tiveram relações com parceiros casuais, sendo que 199 (63,58%) informaram ter usado o preservativo. Entre os participantes, 174(26,61%) tiveram suas práticas sexuais classificadas como adequadas/satisfatórias. Conclusão: os achados evidenciam que os universitários investigados apresentam um comportamento de risco para Infecções Sexualmente Transmissíveis decorrente de práticas sexuais inadequadas/insatisfatórias. Ações de educação em saúde devem considerar aspectos culturais e individuais do grupo para favorecer a reflexão sobre as práticas de prevenção de doenças transmitidas pelo sexo.


Subject(s)
Humans , Adult , Primary Prevention , Risk-Taking , Sexual Behavior , Sexually Transmitted Diseases , Young Adult , Sexual Health
16.
Nursing (Ed. bras., Impr.) ; 22(258): 3286-3290, nov.2019.
Article in Portuguese | LILACS, BDENF - Nursing | ID: biblio-1052034

ABSTRACT

Objetivo: identificar e analisar a percepção do homem sobre qualidade de vida relacionada à saúde antes da cirurgia oncológica. Método: estudo descritivo, exploratório, qualitativo, realizado com 30 homens adultos em tratamento cirúrgico, em uma instituição pública federal no município do Rio de Janeiro, Brasil. Aprovação CEP-INCA-CAAE n°52647116430015274. A técnica de coleta de dados se deu no período de maio a julho de 2016, por meio de entrevistas semiestruturadas gravadas em MP3 e apoiadas em um roteiro com questões pertinentes, relacionadas à qualidade de vida e à saúde do homem antes da cirurgia. Utilizou-se a técnica de Análise de Conteúdo de Bardin para investigação dos dados e organização por categorias temáticas. Resultados: emergiu uma categoria temática central denominada "percepção sobre qualidade de vida relacionada à saúde antes da cirurgia", que se caracterizou por dois eixos: atitudes consideradas positivas; e atitudes que levaram o homem ao adoecimento por câncer. Conclusão: a avaliação de qualidade de vida antes da cirurgia torna-se necessária e deve ser incluída no plano de cuidado de enfermagem da saúde do homem.(AU)


Objective: To identify and analyze the perception of men about health-related quality of life before cancer surgery. Method: a descriptive, exploratory, qualitative study conducted with 30 adult men undergoing surgical treatment at a federal public institution in the city of Rio de Janeiro, Brazil. Approval CEP-INCA-CAAE No. 52647116430015274. The data collection technique took place from May to July 2016, through semi-structured interviews recorded in MP3 and supported by a script with pertinent questions related to the quality of life and men's health before surgery. Bardin's Content Analysis technique was used for data investigation and organization by thematic categories. Results: A central thematic category emerged called "health-related perception of quality of life before surgery", which was characterized by two axes: attitudes considered positive; and attitudes that led the man to illness by cancer. Conclusion: The assessment of quality of life before surgery becomes necessary and should be included in the Men's Health Nursing Care Plan.(AU)


Objetivo: identificar y analizar la percepción de los hombres sobre la calidad de vida relacionada con la salud antes de la cirugía del cáncer. Método: estudio descriptivo, exploratorio y cualitativo realizado con 30 hombres adultos sometidos a tratamiento quirúrgico en una institución pública federal de la ciudad de Río de Janeiro, Brasil. Aprobación CEP-INCA-CAAE No. 52647116430015274. La técnica de recopilación de datos tuvo lugar de mayo a julio de 2016, a través de entrevistas semiestructuradas grabadas en MP3 y respaldadas por un guión con preguntas pertinentes relacionadas con la calidad de vida y la salud de los hombres antes de la cirugía. La técnica de análisis de contenido de Bardin se utilizó para la investigación y organización de datos por categorías temáticas. Resultados: surgió una categoría temática central llamada "percepción de la calidad de vida relacionada con la salud antes de la cirugía", que se caracterizó por dos ejes: actitudes consideradas positivas; y actitudes que llevaron al hombre a la enfermedad por cáncer. Conclusión: La evaluación de la calidad de vida antes de la cirugía se hace necesaria y debe incluirse en el Plan de atención de enfermería para hombres.(AU)


Subject(s)
Humans , Male , Quality of Life , Mouth Neoplasms , Men's Health , Surgical Oncology , Health Promotion
17.
rev. cuid. (Bucaramanga. 2010) ; 9(2): 2215-2232, maio-ago. 2018. tab
Article in Portuguese | LILACS, BDENF - Nursing, COLNAL | ID: biblio-963439

ABSTRACT

Resumo Introdução A autonomia profissional corresponde à realização de ações de enfermagem por meio da utilização de habilidades, conhecimentos e atitudes para tomar decisões e resolver situações no seu espaço de atuação. Objetivo analisar as representações sociais da autonomia do enfermeiro para acadêmicos do último período de graduação em enfermagem de uma universidade federal. Materiais e Métodos: Estudo qualitativo, descritivo, exploratório delineado a partir da teoria das representações sociais em sua abordagem processual, realizada com 20 acadêmicos de enfermagem no último período de uma universidade federal localizada no estado do Rio de Janeiro. A coleta de dados se deu através de entrevistas semiestruturadas e a análise das produções discursivas pelo método de análise de conteúdo. Resultados Foram construídas cinco categorias de análise. Discussão Verificou-se que as representações sociais da autonomia profissional dos discentes de enfermagem encontram-se em processo de construção, tendo por fundamento o gerenciamento, as práticas invasivas e a consulta de enfermagem. A autonomia também se relaciona ao cuidado predominantemente individual. Conclusões A representação da autonomia profissional está vinculada a tecnologias atuais e a dimensões históricas da profissão, bem como a necessidade de novos estudos para a compreensão do fenômeno.


Abstract Introduction Professional autonomy corresponds to the performance of nursing actions by using skills, knowledge, and attitudes to make decisions and solve situations in their work setting. Objective to analyze the social representations of the nurse's autonomy for academics of the final graduation period in nursing from a federal university. Materials and Methods: This was a qualitative, descriptive, exploratory study defined from the theory of social representations in its procedural approach, carried out with 20 nursing academics during their last period of a federal university located in the state of Rio de Janeiro, Brazil. Data was collected through semi-structured interviews and discursive productions were analyzed through the content analysis method. Results The study constructed five analysis categories. Discussion It was verified that the social representations of professional autonomy of nursing students are in the construction process, based on management, invasive practices, and nursing consultation. Autonomy is also related to predominantly individual care. Conclusions The representation of professional autonomy is linked to current technologies and to the historical dimensions of the profession, as well as to the need for new studies to understand the phenomena.


Resumen Introducción La autonomía profesional corresponde a la realización de acciones de enfermería por medio de la utilización de habilidades, conocimientos y actitudes para tomar decisiones y resolver situaciones en su espacio de actuación. Objetivo analizar las representaciones sociales de la autonomía del enfermero con estudiantes de último semestre de enfermería de una universidad federal. Materiales y Métodos: Estudio cualitativo, descriptivo, exploratorio, diseñado a partir de la teoría de las representaciones sociales con enfoque procesal, realizado con 20 alumnos de último semestre de una universidad federal localizada en el estado de Rio de Janeiro. La recolección de datos se dio a través de entrevistas semiestructuradas y el análisis de las producciones discursivas por el método de análisis de contenido. Resultados Fueron construidas cinco categorías de análisis. Discusión Se verificó que las representaciones sociales de la autonomía profesional de los alumnos de enfermería se encuentran en proceso de construcción teniendo por base el gerenciamiento, las prácticas invasivas y la consulta de enfermería. La autonomía también se relaciona al cuidado predominantemente individual. Conclusiones La representación de la autonomía profesional está vinculada a las tecnologías actuales y las dimensiones históricas de la profesión, así como la necesidad de nuevos estudios para la comprensión del fenómeno.


Subject(s)
Humans , Male , Female , Education, Nursing , Nursing Care , Social Perception , Professional Autonomy
18.
Rev Gaucha Enferm ; 38(3): e0006, 2018 Mar 12.
Article in Portuguese, English | MEDLINE | ID: mdl-29538604

ABSTRACT

OBJECTIVE: To analyze the maternal perception about the paternal care and to understand how this care Is made effective In practical actions in the care of the child/adolescent with chronic disease in the family routine. METHODS: Exploratory-descriptive, qualitative study, carried out at the pediatric outpatient clinic of a public hospital in the state of Paraíba, between November/2013 and April/2014, with 10 mothers whose children had more than six months of diagnosis. Semi-structured interview subsidized the data collection, which was submitted to thematic analysis. RESULTS: Parents participated in activities such as routine care of the child, medication management, leisure, binomial support and household chores. They took on different roles in the care depending on the availability of time and the maternal freedom given for their participation. Among the separated couples, the distance between parents in the care reflected on the child's health. CONCLUSION: It is important to involve fathers in the care of the child with chronic diseases. However, there is a need for a greater involvement of fathers in the performance of this care.


Subject(s)
Child Care , Chronic Disease , Fathers , Mothers/psychology , Parent-Child Relations , Adolescent , Brazil , Child , Child Care/psychology , Female , Humans , Interviews as Topic , Male , Qualitative Research
19.
Korean J Parasitol ; 56(1): 11-19, 2018 Feb.
Article in English | MEDLINE | ID: mdl-29529845

ABSTRACT

In Brazil, visceral leishmaniasis (VL) is expanding and becoming urbanized, especially in non-endemic areas such as the State of Rio Grande do Sul. Considering that infected dogs are the main reservoir for zoonotic VL, this study evaluated the prevalence of canine visceral leishmaniasis (CVL) in the metropolitan area of Porto Alegre, a new area of expansion of VL in Brazil. Serum and plasma from 405 asymptomatic dogs from the municipalities of Canoas (n=107), São Leopoldo (n=216), and Novo Hamburgo (n=82) were tested for CVL using immunochromatographic (DPP®) and ELISA EIE® assays (2 assays officially adopted by the Brazilian government for the diagnosis of CVL) and real-time PCR to confirm the results. There was no agreement among serological and real-time PCR results, indicating that the Leishmania infection in asymptomatic animals with low parasite load, confirmed by negative parasitological tests (smears and parasite culture), need to be evaluated by molecular methods. The prevalence of LVC in the metropolitan region of Porto Alegre, confirmed by real-time PCR was 4% (5.6% in Canoas and 4.6% in São Leopoldo). The use of molecular method is essential for accurate diagnosis of CVL, especially in asymptomatic dogs in non-endemic areas.


Subject(s)
Antibodies, Protozoan/blood , Dog Diseases/diagnosis , Dog Diseases/parasitology , Leishmaniasis/diagnosis , Leishmaniasis/veterinary , Parasitology/methods , Animals , Biomarkers/blood , Brazil/epidemiology , Chromatography, Affinity , Cross-Sectional Studies , DNA, Protozoan/isolation & purification , Dog Diseases/epidemiology , Dogs , Enzyme-Linked Immunosorbent Assay , Leishmania infantum/immunology , Leishmaniasis/epidemiology , Prevalence , Real-Time Polymerase Chain Reaction
20.
Epidemiol Infect ; 146(5): 571-576, 2018 04.
Article in English | MEDLINE | ID: mdl-29477155

ABSTRACT

Although serological assays have been widely used for the diagnosis of canine visceral leishmaniasis (CVL), they present different performances depending on the clinical profile of the dogs. This study evaluated the accuracy of serological tests, immunochromatographic (Dual Path Platform: DPP®) and enzyme-linked immunosorbent (ELISA EIE®), for CVL in relation to the detection of Leishmania DNA through real-time polymerase chain reaction (real-time PCR) in samples from symptomatic and asymptomatic dogs from a non-endemic area in the state of Rio Grande do Sul, Southern Brazil. Serum from 140 dogs (39 symptomatic and 101 asymptomatic) was tested by DPP and ELISA followed by real-time PCR. From a total of 140 samples evaluated, Leishmania DNA was detected by real-time PCR in 41.4% (58/140). Moreover, 67.2% of samples positive in real-time PCR were positive in both DPP and ELISA (39/58), showing moderate agreement between methods. In the symptomatic group, one sample non-reactive in both serological assays was positive in real-time PCR, whereas in the asymptomatic group, 17.8% non-reactive or undetermined samples in serological assays were positive in the molecular method. Leishmania DNA was not detected in 17.9% reactive samples by serological assays from the symptomatic group, and in 3.9% from asymptomatic dogs. Real-time PCR demonstrated greater homogeneity between symptomatic and asymptomatic groups compared with DPP and ELISA. The molecular method can help to establish the correct CVL diagnosis, particularly in asymptomatic dogs, avoiding undesirable euthanasia.


Subject(s)
Chromatography, Affinity/veterinary , Dog Diseases/diagnosis , Enzyme-Linked Immunosorbent Assay/veterinary , Leishmania infantum/isolation & purification , Leishmaniasis, Visceral/diagnosis , Real-Time Polymerase Chain Reaction/veterinary , Animals , Asymptomatic Infections , Brazil , Chromatography, Affinity/methods , Dog Diseases/parasitology , Dogs , Enzyme-Linked Immunosorbent Assay/methods , Leishmaniasis, Visceral/parasitology , Real-Time Polymerase Chain Reaction/methods
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