Moving forward in microplastic research: A Norwegian perspective.

Given the increasing attention on the occurrence of microplastics in the environment, and the potential environmental threats they pose, there is a need for researchers to move quickly from basic understanding to applied science that supports decision makers in finding feasible mitigation measures and solutions. At the same time, they must provide sufficient, accurate and clear information to the media, public and other relevant groups (e.g., NGOs). Key requirements include systematic and coordinated research efforts to enable evidence-based decision making and to develop efficient policy measures on all scales (national, regional and global). To achieve this, collaboration between key actors is essential and should include researchers from multiple disciplines, policymakers, authorities, civil and industry organizations, and the public. This further requires clear and informative communication processes, and open and continuous dialogues between all actors. Cross-discipline dialogues between researchers should focus on scientific quality and harmonization, defining and accurately communicating the state of knowledge, and prioritization of topics that are critical for both research and policy, with the common goal to establish and update action plans for holistic benefit. In Norway, cross-sectoral collaboration has been fundamental in supporting the national strategy to address plastic pollution. Researchers, stakeholders and the environmental authorities have come together to exchange knowledge, identify knowledge gaps, and set targeted and feasible measures to tackle one of the most challenging aspects of plastic pollution: microplastic. In this article, we present a Norwegian perspective on the state of knowledge on microplastic research efforts. Norway's involvement in international efforts to combat plastic pollution aims at serving as an example of how key actors can collaborate synergistically to share knowledge, address shortcomings, and outline ways forward to address environmental challenges.

Occurrence and trophic transport of organic compounds in sedimentation ponds for road runoff.

Sedimentation ponds have been shown to accumulate several groups of contaminants, most importantly polycyclic aromatic compounds (PACs) and metals. But also, other urban organic pollutants have shown to be present, including polybrominated diphenyl ethers (PBDEs), organophosphate compounds (OPCs) and benzothiazoles (BTs). This investigation aimed at determining the occurrence of these four groups of contaminants in sedimentation ponds and determine their transport from water/sediment to organisms. PACs, including alkylated PACs, PBDEs; OPCs and BTs were determined in water, sediment, plants, dragonfly larvae and fish from two sedimentation ponds and one reference site. Fish were analysed for PAC metabolites. Overall, higher concentrations of all four pollutant groups were detected in water and sediment from sedimentation ponds compared to two natural lakes in rural environments (reference sites). The concentration difference was highest in sediments, and >20 higher concentration was measured in sedimentation ponds (3.6-4.4 ng/g ww) compared to reference (0.2 ng/g ww) for sum BDE6. For PACs and PBDEs a clear transport from water/sediment to organisms were observed. Fish were the highest trophic level organism (3.5-5) in our study, and all four pollutant groups were detected in fish. For PBDEs a trophic biomagnification (TMF) was found both in sedimentation ponds and reference, but higher concentrations in all matrices were measured in sedimentation ponds. TMF was not calculated for PACs since they are metabolised by vertebrates, but a transfer from water/sediment to organisms was seen. For BTs and OPCs, no consistent transfer to plants and dragonfly larvae could be seen. One OPC and two BTs were detected in fish, but only in fish from sedimentation ponds. It is therefore concluded that sedimentation ponds are hotspots for urban and traffic related contaminants, of which especially PACs and PBDEs are transferred to organisms living there.

The organic pollutant status of rivers in Bosnia and Herzegovina as determined by a combination of active and passive sampling methods.

There is an overall lack of data concerning the pollution status of Bosnia Herzegovina, which is confounded by fragmented national environmental management. The present study aimed to provide some initial data for concentrations of priority substances in two major Bosnian Rivers, using two types of passive sampler (PS) as well as by using high volume water sampling (HVWS). Overall, concentrations of most persistent organic pollutants (POPs), including polychlorinated biphenyls (PCBs) and legacy pesticides, were shown to be low. However, around the town of Doboj on the Bosna River, concentrations of polycyclic aromatic hydrocarbons (PAH) breached European standards for several compounds and reached 67 ng L-1 for freely dissolved concentrations and 250 ng L-1 for total concentrations. In general, contamination was lower in the Neretva River compared to the Bosna, although for brominated diphenyl ethers (PBDEs), results suggested an active source of PBDEs at one location based on the ratio of congeners 47 and 99. Direct comparisons between the different sampling techniques used are not straightforward, but similar patterns of PAH contamination were shown by HVWS and PS in the Bosna River. There are both scientific and practical considerations when choosing which type of sampling technique to apply, and this should be decided based on the goals of each individual study.

Roads and motorized transport as major sources of priority substances? A data register study.

A data register study was performed in order to identify the amounts of hazardous substances in products related to motorized transport in Norway during 2012. The hazardous substances were selected from legislative investigations performed by the European Chemicals Agency (ECHA), European Union (EU), and Norwegian Environment Agency (NEA). Information regarding hazardous substances in 52 selected product categories associated with traffic-related activities was obtained from the Norwegian Product Register administrated by the NEA. Substances present on ECHA list of substances of very high concern (SVHC), NEA national priority list, and priority substances under the EU Water Framework Directive (WFD) were given most attention, with substances from ECHA community rolling action plan (CoRAP) also included. Results showed that selected products contained a diverse range of substances that were classified as hazardous to either human or environmental health. The quantities of hazardous substances in the selected products were 120 tons (SVHC), 280 tons (Norway priority list), and 2,400 tons (WFD). It proved difficult to pinpoint these quantities only to traffic-related operations since product categories included compounds used for other activities. However, data illustrate that large quantities of hazardous substances are employed concurrent with being prioritized for reduction/elimination by national and international authorities. A list of substances with annual use in 2012 >1 ton was prepared to aid a prioritization for further actions such as substitution, phasing out, or environmental monitoring. The list contains substances that are toxic to humans, especially as adverse reproductive/carcinogenic agents, and/or pose a threat to the environment.

Identification of non-regulated polycyclic aromatic compounds and other markers of urban pollution in road tunnel particulate matter.

A combination of silicone rubber extraction and non-target and suspect screening by gas chromatography coupled to high-resolution time-of flight mass spectrometry was used for the identification of compounds in particulate matter (PM). Tunnel PM is a proxy for local road pollution that constitutes a hazard to the urban environment and human health. The use of silicone rubber for the extraction of PM allowed the pre-concentration of a wide range of compounds for non-target analysis while minimising the effects of the sample matrix. As expected, polycyclic aromatic compounds (PACs) constituted the major group of compounds identified, but only 5 of 50 PACs identified were amongst those regularly monitored and many of them were alkylated or contained a heteroatom. Urban markers of contamination such as organophosphate flame-retardants, phthalates, benzothiazoles, musk compounds and a plasticiser were also identified. The level of confidence for the identifications was high based on accurate mass, the pattern of fragmentation and retention. The unequivocal identification of 16 compounds, from all groups, was confirmed by co-chromatography with standards and the compounds semi-quantified. Most of the PACs identified are not regularly monitored, and the hazards they pose are therefore unknown. Some of these PACs are known to be more persistent and mobile in the environment than the EPA PAH16.

In vivo and in vitro effects of tunnel wash water and traffic related contaminants on aquatic organisms.

In order to maintain the construction and safety of road tunnels, they are routinely washed. The wash water appears to be highly polluted with a plethora of contaminants in elevated concentrations. In addition, new and emerging compounds are likely to occur. The discharge water has shown acute toxic and sub-lethal effects in several organisms. In this study, ecotoxicity tests with algae (Pseudokirchneriella subcapitata) and in vitro tests with primary rainbow trout (Oncorhynchus mykiss) hepatocytes were used to characterize the effect of TWW from three different tunnels. In addition, selected N- and Cl-PAHs were tested for cytotoxicity, EROD activity and CYP1A protein production. TWW samples and/or extracts from two tunnels reduced the algal growth and induced cytotoxicity, EROD activity and CYP1A protein production in vitro. Four of the eight tested Cl- and N-substituted PAHs induced EROD activity and CYP1A protein production at micro-molar concentrations. N-PAHs were detected in samples from the tunnel wash, highlighting substituted PAHs as potentially important traffic-related contaminants.

PAH related effects on fish in sedimentation ponds for road runoff and potential transfer of PAHs from sediment to biota.

Road runoff is an important source of pollution to the aquatic environment, and sedimentation ponds have been installed to mitigate effects on the aquatic environment. The purpose of this study was to investigate if a) fish from sedimentation ponds were affected by road pollution and; b) the transfer of PAHs from road runoff material to aquatic organisms was substantial. Minnow from a sedimentation pond (Skullerud) near Oslo (Norway) had higher levels of CYP1A enzyme and DNA stand breaks than minnow from the nearby river, but high concentrations of PAH-metabolites in bile revealed that both populations were highly exposed. Principal component analysis revealed that CYP1A and age of fish were correlated, while levels of PAH-metabolites were not correlated to CYP1A or DNA damage. Minnow from a lake un-affected by traffic had much lower levels of PAH-metabolites than the exposed fish, and also an improved condition. The latter results indicate that fish health was affected by road runoff. A closer investigation of PAH levels of the ecosystems of two sedimentation ponds (Skullerud and Vassum) and nearby environments were conducted. The concentration of the 16 EPA PAHs in sediments of the sedimentation ponds were high (1900-4200ngg(-1)), and even higher levels were observed in plants. Principal component analysis of selected ion chromatograms of PAHs showed a clear separation of plants vs. sediments. The plants preferentially accumulated the high molecular PAHs, both from sedimentation ponds with a petrogenic PAH isomer ratio in sediments; and from a lake with pyrogenic PAH isomer ratio in sediments.

PAH Accessibility in Particulate Matter from Road-Impacted Environments.

Snowmelt, surface runoff, or stormwater releases in urban environments can result in significant discharges of particulate matter-bound polycyclic aromatic hydrocarbons (PAHs) into aquatic environments. Recently, more-specific activities such as road-tunnel washing have been identified as contributing to contaminant load to surface waters. However, knowledge of PAH accessibility in particulate matter (PM) of urban origin that may ultimately be released into urban surface waters is limited. In the present study, we evaluated the accessibility of PAHs associated with seven distinct (suspended) particulate matter samples collected from different urban sources. Laboratory-based infinite sink extractions with silicone rubber (SR) as the extractor phase demonstrated a similar pattern of PAH accessibility for most PM samples. Substantially higher accessible fractions were observed for the less-hydrophobic PAHs (between 40 and 80% of total concentrations) compared with those measured for the most-hydrophobic PAHs (<5% of total concentrations). When we focused on PAHs bound to PM from tunnel-wash waters, first-order desorption rates for PAHs with log Kow > 5.5 were found in line with those commonly found for slowly or very slowly desorbing sediment-associated contaminants. PAHs with log Kow < 5.5 were found at higher desorbing rates. The addition of detergents did not influence the extractability of lighter PAHs but increased desorption rates for the heavier PAHs, potentially contributing to increases in the toxicity of tunnel-wash waters when surfactants are used. The implications of total and accessible PAH concentrations measured in our urban PM samples are discussed in a context of management of PAH and PM emission to the surrounding aquatic environment. Although we only fully assessed PAHs in this work, further study should consider other contaminants such as OPAHs, which were also detected in all PM samples.

Passive sampling for target and nontarget analyses of moderately polar and nonpolar substances in water.

The applicability of silicone rubber and low-density polyethylene (LDPE) as passive sampling materials for target and nontarget analyses of moderately polar and nonpolar substances was assessed through a field deployment of samplers along a small, polluted stream in Oslo, Norway. Silicone and LDPE samplers of identical surface area (but different volumes) were deployed at 6 sites in the River Alna for 49 d. Quantitative target analysis by gas chromatography-mass spectrometry (quadrupole, single-ion monitoring mode) demonstrated that masses of polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and organochlorine compounds absorbed in the 2 polymeric materials were consistent with the current understanding of the control and mode of accumulation in these sampler materials. Some deviation was observed for decabromodiphenyl ether (BDE-209) and may be linked to the large molecular size of this substance, resulting in lower diffusivity in the LDPE. Target and nontarget analyses with gas chromatography coupled to high resolution time-of-flight mass spectrometry allowed the identification of a wide range of chemicals, including organophosphate compounds (OPCs) and musk compounds (galaxolide and tonalid). Semiquantitative analysis revealed enhanced quantities of the OPCs in silicone material, indicating some limitation in the absorption and diffusion of these substances in LDPE. Overall, silicone allows nontarget screening analysis for compounds with a wider range of log octanol-water partition coefficient values than what can be achieved with LDPE.

Screening for Stockholm Convention persistent organic pollutants in the Bosna River (Bosnia and Herzogovina).

The Stockholm Convention, which aspires to manage persistent organic pollutants (POPs) at the international level, was recently ratified in Bosnia and Herzegovina (BiH). Despite this fact, there is in general a paucity of data regarding the levels of POPs in the environment in BiH. In the present study, screening for POPs was conducted in one of the country's major rivers, the Bosna. A two-pronged approach was applied using passive samplers to detect the freely dissolved and bioavailable concentrations in the water phase and sediment analysis to provide an integrated measure of historical contamination. At several places along the river, the concentrations of polycyclic aromatic hydrocarbons (PAH) were high and exhibited potential for both chronic and acute effects to biota. River water also showed elevated concentrations of PAH, up to 480 ng L(-1) near the city of Doboj, and diagnostic ratios suggested combustion sources for the contamination present in both types of sample. The levels of the other contaminants measured-polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs) and polybrominated diphenyl ethers--were generally low in the water phase. However, PCBs and some OCPs were present in river sediments at levels which breach the international criteria and thus suggest potential for ecological damage. Additionally, the levels of heptachlor breached these criteria in many of the sites investigated. This study presents the first screening data for some of these Stockholm Convention relevant compounds in BiH and reveals both low concentrations of some chemical groups, but significant point sources and historic contamination for others.

Occurrence of PAHs and PCBs in the Alna River, Oslo (Norway).

Thirty two polychlorinated biphenyl congeners (PCBs), hexachlorobenzene (HCB) and pentachlorobenzene (PeCB) were analysed in passive sampler extracts from surface water-exposed semipermeable membrane devices (SPMDs) and in bed sediment samples from a small urban watercourse, the River Alna (Oslo, Norway). Performance reference compound-corrected data from the passive samplers deployed at three sites along the river were used to track PCB contamination in the overlying water. SPMDs were able to detect an increase in dissolved PCB concentrations at the site furthest downstream that was corroborated by bed sediment concentrations. In comparison, no major increase in concentration of HCB, PeCB or PAHs could be observed. Comparison of passive sampling-based overlying water concentrations with total concentrations measured in bed sediments supports the possibility of further PCB sources upstream of the study area. Diagnostic PAH ratios (from SPMDs) and PCB congener pattern (from sediments) were used in an attempt to identify possible contaminant sources to the Alna River. Selected PAH diagnostic ratios support a multiple emission source scenario and demonstrate the complexity of identifying specific sources of these compounds to surface waters. PCB congener patterns in sediments from all three sites tend to indicate a source of highly chlorinated PCBs (of the Archlor 1260 type) and either a source of lower chlorinated PCBs or the less-likely occurrence of dechlorination in sediment. Information collected during the present screening study also confirms the Alna River as a continuous source of PCBs to the Oslofjord.

Use of phospholipid fatty acids to detect previous self-heating events in stored peat.

The use of the phospholipid fatty acid (PLFA) composition of microorganisms to detect previous self-heating events was studied in naturally self-heated peat and in peat incubated under temperature-controlled conditions. An increased content of total PLFAs was found in self-heated peat compared to that in unheated peat. Two PLFAs, denoted T1 and T2, were detected only in the self-heated peat. Incubation of peat samples at 25 to 55 degrees C for 4 days indicated that T1 and T2 were produced from microorganisms with different optimum temperatures. This was confirmed by isolation of bacteria at 55 degrees C, which produced T2 but not T1. These bacteria produced another PLFA (denoted T3) which coeluted with 18:1omega7. T2 and T3 were identified as omega-cyclohexyltridecanoic acid and omega-cyclohexylundecanoic acid, respectively, indicating that the bacteria belonged to the genus Alicyclobacillus: T1 was tentatively identified as omega-cycloheptylundecanoic acid. T2 was detected 8 h after the peat incubation temperature was increased to 55 degrees C, and maximum levels were found within 5 days of incubation. The PLFA 18:1omega7-T3 increased in proportion to T2. T1 was detected after 96 h at 55 degrees C, and its level increased throughout the incubation period, so that it eventually became one of the dominant PLFAs after 80 days. In peat samples incubated at 55 degrees C and then at 25 degrees C, T1 and T2 disappeared slowly. After 3 months, detectable levels were still found. Incubation at 25 degrees C after heating for 3 days at 55 degrees C decreased the amounts of T2 and 18:1omega7-T3 faster than did incubation at 5 degrees C. Thus, not only the duration and temperature during the heating event but also the storage temperature following heating are important for the detection of PLFAs indicating previous self-heating.