Transcriptomic and Lipidomic Analysis Reveals Complex Regulation Mechanisms Underlying Rice Roots' Response to Salt Stress.

Rice (Oryza sativa L.), a crucial food crop that sustains over half the world's population, is often hindered by salt stress during various growth stages, ultimately causing a decrease in yield. However, the specific mechanism of rice roots' response to salt stress remains largely unknown. In this study, transcriptomics and lipidomics were used to analyze the changes in the lipid metabolism and gene expression profiles of rice roots in response to salt stress. The results showed that salt stress significantly inhibited rice roots' growth and increased the roots' MDA content. Furthermore, 1286 differentially expressed genes including 526 upregulated and 760 downregulated, were identified as responding to salt stress in rice roots. The lipidomic analysis revealed that the composition and unsaturation of membrane lipids were significantly altered. In total, 249 lipid molecules were differentially accumulated in rice roots as a response to salt stress. And most of the major phospholipids, such as phosphatidic acid (PA), phosphatidylcholine (PC), and phosphatidylserine (PS), as well as major sphingolipids including ceramide (Cer), phytoceramide (CerP), monohexose ceramide (Hex1Cer), and sphingosine (SPH), were significantly increased, while the triglyceride (TG) molecules decreased. These results suggested that rice roots mitigate salt stress by altering the fluidity and integrity of cell membranes. This study enhances our comprehension of salt stress, offering valuable insights into changes in the lipids and adaptive lipid remodeling in rice's response to salt stress.

Effect of plant growth regulators DA-6 and COS on drought tolerance of pineapple through bromelain and oxidative stress.

BACKGROUND: Due to global warming, drought climates frequently occur on land, and despite being drought resistant, pineapples are still subjected to varying degrees of drought stress. Plant growth regulators can regulate the stress tolerance of plants through hormonal effects. This experiment aims to investigate the regulatory effects of different plant growth regulators on Tainong- 16 and MD-2 Pineapple when subjected to drought stress. RESULTS: In this experiment, we examined the regulatory effects of two different plant growth regulators, sprayed on two pineapple varieties: MD-2 Pineapple and Tainong-16. The main component of T1 was diethyl aminoethyl hexanoate (DA-6) and that of T2 is chitosan oligosaccharide (COS). An environment similar to a natural drought was simulated in the drought stress treatments. Then, pineapples at different periods were sampled and a series of indicators were measured. The experimental results showed that the drought treatments treated with T1 and T2 plant growth regulators had a decrease in malondialdehyde, an increase in bromelain and antioxidant enzyme indicators, and an increase in phenotypic and yield indicators. CONCLUSION: This experiment demonstrated that DA-6 and COS can enhance the drought resistance of pineapple plants to a certain extent through bromelain and oxidative stress. Therefore, DA-6 and COS have potential applications and this experiment lays the foundation for further research.

Physiological mechanisms of ABA-induced salinity tolerance in leaves and roots of rice.

Abscisic acid (ABA) plays a crucial role in response to abiotic stress as important small molecules in regulating metabolism. This study aimed to evaluate the ability of foliar spraying ABA to regulate growth quality at rice seedling stage under salt stress. Results demonstrated that salt stress strongly reduced all the growth parameters of two rice seedlings ('Chaoyouqianhao' and 'Huanghuazhan'), caused prominent decrease in the levels of photosynthetic pigments (mainly in Huanghuazhan), photosynthesis and fluorescence parameters. Salinity treatment increased the concentration of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in roots, whereas significant decreased H2O2 was found in leaves of Huanghuazhan. Additionally, salinity triggered high Na+ content particularly in leaves and enhanced catalase (CAT) activities, ascorbate peroxidase (APX) and peroxidase (POD) activities of the two rice seedlings. Nevertheless, salinity-induced increased root ascorbic acid (AsA) and glutathione (GSH) levels while decreased in leaves, which depended on treatment time. Conversely, ABA application partially or completely mitigated salinity toxicity on the seedlings. ABA could reverse most of the changed physiological parameters triggered by salt stress. Specially, ABA treatment improved antioxidant enzyme levels and significantly reduced the Na+ content of two varieties as well as increased the K+, Mg2+ and Ca2+ content in leaves and roots. ABA treatment increased the hormone contents of 1-aminocclopropane carboxylic acid (ACC), trans-zeatin (TZ), N6-isopentyladenosine (IPA), Indole-3-acetic acid (IAA), and ABA in leaves of two rice varieties under salt stress. It is suggested that ABA was beneficial to protect membrane lipid peroxidation, the modulation of antioxidant defense systems and endogenous hormonal balance with imposition to salt stress.

Ultrasonic seed treatment improved morpho-physiological and yield traits and reduced grain Cd concentrations in rice.

Rice cultivation under cadmium (Cd) contaminated soil often results in reduced growth with excess grain Cd concentrations. A pot experiment was conducted to assess the potential of ultrasonic seed treatment to alleviate Cd stress in rice. Seeds of two aromatic rice cultivars i.e., Xiangyaxiangzhan and Meixiangzhan 2 and two non-aromatic rice cultivars i.e., Huahang 31 and Guangyan 1 were exposed to ultrasonic waves for 1.5 min in 20-40 KHz mixing frequency. The experimental treatments were comprised of untreated seeds (U0) and ultrasonic treated seeds (U1) transplanted in un-contaminated soil (H0) and Cd-contaminated soil (H1). Results revealed that Cd contents and Cd accumulation in grain in U1 were 33.33-42.31% and 12.86-57.58% lower than U0 for fragrant rice cultivars under H1. Meanwhile, biomass production was higher in U1 than U0 under H0 and better yield was assessed in U1 for all cultivars under H1. The activity of peroxidase (POD) in flag leaves was increased by 8.28-115.65% for all cultivars while malondialdehyde (MDA) contents were significantly decreased in U1 compared with U0 under H0. Conclusively, ultrasonic treatment modulated Cd distribution and accumulation in different parts while improved physiological performance as well as yield and grain quality of rice under Cd contaminated conditions.

Application of inorganic passivators reduced Cd contents in brown rice in oilseed rape-rice rotation under Cd contaminated soil.

Immobilization of heavy metals by the application of chemical amendments is an eco-friendly, economical and effective method to remediate cadmium (Cd) -contaminated soils. Field experiments were conducted during 2016-2018 following oilseed rape-rice rotation with the application of inorganic passivators i.e., slaked lime (CaOH2>95%) and sepiolite (SiO2>50%, MgO>20%). The experimental treatments were comprised of: rice planting in winter fallow and without passivator (F-PA0); fallow with slaked lime (F-PA1); fallow with sepiolite (F-PA2); oilseed rape-rice rotation without passivator (R-PA0); rotation with slaked lime (R-PA1); and rotation with sepiolite (R-PA2). The slaked lime and sepiolite were applied after the harvest of rape at 2000 and 5000 kg ha-1, respectively. Results revealed that the Cd contents were reduced by 47.44-49.03% in brown rice for F-PA2 and 9.54-42.66% in soil for R-PA2. The superoxide dismutase (SOD) activity was decreased by 10.65-17.98%, the malondialdehyde (MDA) contents were reduced by 28.57% whereas the proline content was decreased by 32.61% under R-PA2. In addition, the filled grain percentage was improved by 6.87% (F-PA2) and 3.70% (R-PA1), respectively. Overall, rice sown after oilseed rape gave better yields than sown after fallow fields while application of slaked lime and sepiolite as passivator could be a potential management option to grow crops in metal polluted soils.

Ultrasonic seed treatment improved cadmium (Cd) tolerance in Brassica napus L.

Cadmium (Cd) affects crop growth and productivity by disrupting normal plant metabolism. To determinate whether ultrasonic (US) seed treatment can alleviate Cd stress in rape (Brassica napus L.), the seeds of two oilseed rape cultivars i.e., 'Youyanzao18' and 'Zaoshu104' were exposed to ultrasonic waves for 1 min at 20 KHz frequency. Seeds without US treatment were taken as control (CK). Results revealed that the germination rate of both cultivars was significantly (P < 0.05) higher in US treatment than CK only at 0 and 10 mg Cd L-1. The shoot and root length of both cultivars were significantly higher in US treatment than CK at all Cd treatments except the root length of Youyanzao18 at 50 mg Cd L-1. The fresh weight Youyanzao18 was significantly (P < 0.05) higher in US than CK except for Youyanzao18 at 25 mg Cd L-1. Moreover, the superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) activities and the proline, glutathione (GSH), and soluble protein contents in Youyanzao18 were relatively higher in the US treatment than CK. The malondialdehyde (MDA) contents were prominently reduced in US treatment than CK. The pods per plant, seeds per pod and rapeseed yield were increased by 15.9, 11.4, and 16.4% in Youyanzao18 and 10.3, 9.5, and 11.5% in Zaoshu104, respectively in US treatment, compared to CK. Moreover, the contents of Cd in root, stem, leaf, rape pod shell, and rapeseeds were comparatively less in US treatment than CK whereas the Cd concentrations in different plant parts of both rape cultivars were recorded as: leaf ˃ root ˃ stem ˃ rape pod shell ˃ rapeseed. In sum, the US treatment improved the morphological growth and rapeseed yield whereas reduced the Cd accumulation in different plant parts of rapeseed under Cd contaminated soil.

Ultrasonic seed treatment improved physiological and yield traits of rice under lead toxicity.

Lead (Pb) is a highly toxic metal that damages normal plant metabolism and often results in reduced crop growth and yield. To measure whether or not ultrasonic seed (US) treatment alleviates Pb stress in rice, the seeds of two rice cultivars, i.e., Guangyan1 and Huahang31, were exposed to 30 min of ultrasonic vibration and then the seedlings were transplanted to Pb-contaminated soil. Results revealed that the Pb contents in roots, stems, leaves, panicles (at heading), and brown rice (at maturity) were lower in US treatment than control (CK; without US treatment); the trend for accumulation of Pb in different plant parts was recorded as: root ˃ stems ˃ leaves ˃ panicles ˃ brown rice in both rice cultivars. Overall, the Pb contents in the brown rice of Huahang31 were higher than those in Guangyan1. Moreover, the contents of proline and soluble protein as well as the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) were significantly higher while the malondialdehyde (MDA) contents were significantly reduced in US treatment compared to CK. The effective panicle number, seed set percentage, grains per panicle, 1000-grain weight, and grain yield were increased by 8.9%, 5.3%, 6.8%, 4.4%, and 26.6% in Guangyan1 and 3.2%, 5.7%, 5.7%, 5.0%, and 24.2% in Huahang31, respectively in US treatment compared to CK. Hence, seed treatment with ultrasonic waves could improve rice performance and reduce brown rice Pb accumulation under Pb-polluted soils.