Staphylococcus edaphicus KCB02A11 incorporated with natural adsorbents: first report on its tolerance and removal of hexavalent chromium [Cr(VI)].

Deteriorating the quality of different parts of the ecosystem due to toxic metals is a serious global issue. Hexavalent chromium is a metal that can cause adverse effects on all living beings, including plants, animals, and microorganisms, on exposure to high concentrations for prolonged periods. Removing hexavalent chromium from various types of wastes is challenging; hence the present study investigated the use of bacteria incorporated with selected natural substrates in removing hexavalent chromium from water. Isolated Staphylococcus edaphicus KCB02A11 has shown higher removal efficiency with a wide hexavalent chromium range (0.025-8.5 mg/L) within 96 h. Incorporating the isolated strain with natural substrates commonly found in the environment (hay and wood husk) showed high removal potential [100% removal with 8.5 mg/L of Cr(VI)], even within less than 72 h, with the formation of biofilms on the used substrates applied for metal removal on a large scale for prolonged periods. This study is the first report investigating hexavalent chromium tolerance and removal by Staphylococcus edaphicus KCB02A11.

Sol-Gel Immobilized Optical Microalgal Biosensor for Monitoring Cd, Cu and Zn Bioavailability in Freshwater.

While analytical measurements provide the quantitative estimation of the total amount of metals present in a sample, they do not reflect the truly bioavailable fraction of metal which reflects the adverse biological effect. Hence the development of monitoring tools for detecting bioavailable toxic metals has become a priority in environmental monitoring activities. An optical whole-cell biosensor was constructed using the microalga Scenedesmus subspicatus MM1 immobilizing in inorganic silica hydrogels using the sol-gel technique to detect bioavailable Cadmium (Cd2+), Copper (Cu2+) and Zinc (Zn+) in freshwater. Conditions for optimum biosensor performance have been established regarding effective pH range, cell density, exposure time, and storage stability. The optimum response for the biosensor was dependent on the pH of the matrix, cell concentration and exposure time were derived. The biosensor was operational for four weeks. The limit of detection for the algal biosensor was determined as 9.0 × 10-1, 9.1 × 10-1, and 8.8 × 10-1 mg/L for Cd, Cu and Zn, respectively. Whole-cell cell biosensor will be highly useful since it comprises a single microalgal species able to detect the bioavailable content of Cd2+, Cu2+, and Zn2+ in freshwater.

Potential use of Chlorella vulgaris KCBAL01 from a freshwater stream receiving treated textile effluent in hexavalent chromium [Cr(VI)] removal in extremely acidic conditions.

Remediation of hexavalent chromium with conventional chemical and physical methods is a costly process, while replacing some critical steps in physiochemical remediation with self-sustaining bioremediation agents are expected to be cost-effective and environmentally friendly implementation. In this study, a microalga isolated from a freshwater stream receiving treated textile wastewater was identified up to its molecular level and investigated its ability to tolerate and remove hexavalent chromium from extremely acidic conditions under different temperatures. The ability of microalgae to tolerate and remove Cr(VI) was investigated by growing it in BG11 media with different pH (1, 2, 3 & 7), amended with several concentrations of Cr(VI) and incubated under different temperatures for 96 hrs. Microalga was identified as Chlorella vulgaris and found that the isolated strain has a higher hexavalent chromium removal potential in extremely acidic conditions than in neutral pH conditions at 25 °C. In contrast, its Cr(VI) removal potential is significantly influenced by the pH and temperature of the growth medium. Furthermore, it exhibited a permanent viability loss at extreme acidic conditions (pH 1 - 3) and prolonged exposure to the higher chromium content. The microalga investigated will be a highly useful bioagent in hexavalent chromium remediation in high acidic conditions.

Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems.

Bioavailable content of metals in aquatic systems has become critical in assessing the toxic effect of metals accumulating in the environment. Considering the need for rapid measurements, an optical microalgal-cyanobacterial array biosensor was developed using two strains of microalgae, Mesotaenium sp. and a strain of cyanobacteria Synechococcus sp. to detect Cd2+, Cr6+ and Zn2+ in aquatic systems. Microalgal and cyanobacterial cells were immobilized in a 96-well microplate using sol-gel method using silica. Optimum operational conditions for the biosensor array such as exposure time, storage stability, pH, and multiple metal effect were tested. A 10 min exposure time yielded optimum fluorescence values. Metal toxicity increased with decreasing pH, resulting in low relative fluorescence (%) and decreased with increasing pH, resulting in higher relative fluorescence (%). The optimum storage time for biosensor strains were 4 weeks for microalgal cultures and 8 weeks for cyanobacterial culture, at 4 °C storage temperature. The metal mixtures showed less effect on the inhibition of relative fluorescence (%) of microalgal/cyanobacterial cultures, displaying an antagonistic behavior among the metals tested. As a single unit, this photosynthetic array biosensor will be a valuable tool in detecting multi-metals in aquatic systems.

Comparison of Sensitivity of Tropical Freshwater Microalgae to Environmentally Relevant Concentrations of Cadmium and Hexavalent Chromium in Three Types of Growth Media.

Sensitivity of tropical freshwater microalgae (Mesotaenium sp., Chlorococcum sp. and Scenedesmus sp.) to environmentally relevant concentrations of hexavalent chromium (Cr6+) and cadmium (Cd2+) was compared individually in three growth media viz. Bold's Basal Medium (BBM), Test Medium 1 (TM1) and Test Medium 2 (TM2) based on fluorescence reduction. Free metal content of growth media was determined by Visual MINTEQ (version 3.1). After 24 h, relative fluorescence of microalgae in the three media decreased with increased metal concentration showing a concentration dependent graded toxicity response. All microalgae were more sensitive to the metals when grown in TM1, when compared, more sensitive to Cr6+ than Cd2+. Metal speciation indicated that TM1 and TM2 media have higher percentage of bioavailable Cd2+ than BBM, and chromium was present mainly as CrO42- and HCrO4-. The results suggest that the TM1 medium is more suitable under short term exposure of microalgae to metals in environmental monitoring.

Toward Sustainable Environmental Quality: Priority Research Questions for Asia.

Environmental and human health challenges are pronounced in Asia, an exceptionally diverse and complex region where influences of global megatrends are extensive and numerous stresses to environmental quality exist. Identifying priorities necessary to engage grand challenges can be facilitated through horizon scanning exercises, and to this end we identified and examined 23 priority research questions needed to advance toward more sustainable environmental quality in Asia, as part of the Global Horizon Scanning Project. Advances in environmental toxicology, environmental chemistry, biological monitoring, and risk-assessment methodologies are necessary to address the adverse impacts of environmental stressors on ecosystem services and biodiversity, with Asia being home to numerous biodiversity hotspots. Intersections of the food-energy-water nexus are profound in Asia; innovative and aggressive technologies are necessary to provide clean water, ensure food safety, and stimulate energy efficiency, while improving ecological integrity and addressing legacy and emerging threats to public health and the environment, particularly with increased aquaculture production. Asia is the largest chemical-producing continent globally. Accordingly, sustainable and green chemistry and engineering present decided opportunities to stimulate innovation and realize a number of the United Nations Sustainable Development Goals. Engaging the priority research questions identified herein will require transdisciplinary coordination through existing and nontraditional partnerships within and among countries and sectors. Answering these questions will not be easy but is necessary to achieve more sustainable environmental quality in Asia. Environ Toxicol Chem 2020;39:1485-1505. © 2020 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Sensitivity of Four Cyanobacterial Isolates from Tropical Freshwaters to Environmentally Realistic Concentrations of Cr(6+), Cd(2+) and Zn(2.).

Sensitivity of four tropical cyanobacteria viz. Coelosphaerium sp., Synechococcus sp., Oscillatoria sp. and Chroococcus sp. to environmentally relevant concentrations of Cr(6+), Cd(2+) and Zn(2+)was assessed based on fluorescence change as a proxy for growth reduction. At 24 h exposure, the growth reduction inthe cyanobacteria followed the order: Zn(2+) < Cr(6+) ≤ Cd(2+). Of the four cyanobacteria, Synechococcus was the most sensitive for Cr(6+), where as Chroococcus was the most sensitive for Cd(2+)and Zn(2+). Sensitivity was gradually decreased by 96 h implying the acquisition of tolerance by cyanobacteria to heavy metal ions with prolonged exposure.

Heavy metal toxicity to bacteria - are the existing growth media accurate enough to determine heavy metal toxicity?

A new minimal medium was formulated considering the limitations of the existing media for testing heavy metal sensitivity to bacteria. Toxicity of cadmium and copper to three bacteria was investigated in the new medium and compared with three other media commonly used to study the effect of the toxic metals. Based on speciation data arrived at using ion-selective electrodes, the available free-metal concentration in solution was highest in the MES-buffered medium. This finding was strongly supported by the estimated EC(50) values for the metals tested based on the toxicity bioassays. The free-ionic cadmium and copper concentrations in the medium provide more accurate determination of metal concentrations that affects the bacteria, than with most of other existing media. This will avoid doubts on other media and misleading conclusions relevant to the toxicity of heavy metals to bacteria and provides a better option for the study of metal-bacteria interactions.