ABSTRACT
Engagement of MHC class I-specific inhibitory receptors regulates natural killer (NK) cell development and function. Using both new and previously characterized anti-Ly49 monoclonal antibodies, we comprehensively determined expression and co-expression frequencies of four Ly49 receptors by NK cells from MHC-congenic, MHC class I-deficient, and Ly49A-transgenic mice to study mechanisms that shape the inhibitory Ly49 repertoire. All Ly49 receptors were expressed on partially overlapping subsets. Significantly, in the absence of class I MHC, several receptor pairs were co-expressed more frequently than predicted from a purely random expression model, indicating that biases independent of MHC class I underlie receptor co-expression in some cases. MHC interactions were found to inhibit Ly49 co-expression variably depending on the MHC allele and the receptor pair examined. These data extend previous evidence that interactions with MHC shape the repertoire. It was previously proposed that developing NK cells express Ly49 receptors sequentially and cumulatively, until self-MHC specific receptors are expressed and inhibit new receptor expression. Fulfilling a major prediction of this model, we found that class I recognition by a Ly49A transgene expressed by all developing NK cells equivalently inhibited expression of endogenous self-specific and nonself-specific Ly49 receptors.
Subject(s)
Antigens, Ly , Histocompatibility Antigens Class I/physiology , Killer Cells, Natural/metabolism , Membrane Glycoproteins/analysis , Animals , Lectins, C-Type , Membrane Glycoproteins/genetics , Membrane Glycoproteins/physiology , Mice , Mice, Inbred C57BL , Mice, Transgenic , Models, Biological , NK Cell Lectin-Like Receptor Subfamily A , Receptors, NK Cell Lectin-LikeABSTRACT
Viral infections are often accompanied by extensive proliferation of reactive CD8 T cells. After a defined number of divisions, normal somatic cells enter a nonreplicative stage termed senescence. In the present study we have identified the inhibitory killer cell lectin-like receptor G1 (KLRG1) as a unique marker for replicative senescence of murine CD8 T cells. KLRG1 expression was induced in a substantial portion (30-60%) of CD8 T cells in C57BL/6 mice infected with lymphocytic choriomeningitis virus (LCMV), vesicular stomatitis virus, or vaccinia virus. Similarly, KLRG1 was found on a large fraction of LCMV gp33 peptide-specific TCR-transgenic (tg) effector and memory cells activated in vivo using an adoptive transfer model. Transfer experiments with CFSE-labeled TCR-tg cells into LCMV-infected hosts further indicated that induction of KLRG1 expression required an extensive number of cell divisions. Most importantly, KLRG1(+) TCR-tg effector/memory cells could efficiently lyse target cells and secrete cytokines, but were severely impaired in their ability to proliferate after Ag stimulation. Thus, this study demonstrates that senescent CD8 T cells are induced in abundant numbers during viral infections in vivo.
Subject(s)
CD8-Positive T-Lymphocytes/physiology , Cellular Senescence , Lectins, C-Type , Membrane Glycoproteins , Virus Diseases/immunology , Aging/immunology , Animals , Cell Division , Lymphocytic Choriomeningitis/immunology , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Receptors, Immunologic/analysis , Vaccinia virus , Vesicular stomatitis Indiana virusABSTRACT
Natural killer (NK) cells attack many tumour cell lines, and are thought to have a critical role in anti-tumour immunity; however, the interaction between NK cells and tumour targets is poorly understood. The stimulatory lectin-like NKG2D receptor is expressed by NK cells, activated CD8+ T cells and by activated macrophages in mice. Several distinct cell-surface ligands that are related to class I major histocompatibility complex molecules have been identified, some of which are expressed at high levels by tumour cells but not by normal cells in adults. However, no direct evidence links the expression of these 'induced self' ligands with tumour cell rejection. Here we demonstrate that ectopic expression of the murine NKG2D ligands Rae1beta or H60 in several tumour cell lines results in potent rejection of the tumour cells by syngeneic mice. Rejection is mediated by NK cells and/or CD8+ T cells. The ligand-expressing tumour cells induce potent priming of cytotoxic T cells and sensitization of NK cells in vivo. Mice that are exposed to live or irradiated tumour cells expressing Rae1 or H60 are specifically immune to subsequent challenge with tumour cells that lack NKG2D ligands, suggesting application of the ligands in the design of tumour vaccines.
Subject(s)
CD8-Positive T-Lymphocytes/immunology , Killer Cells, Natural/immunology , Membrane Proteins/metabolism , Minor Histocompatibility Antigens/metabolism , Neoplasms/immunology , Receptors, Immunologic/metabolism , Animals , Cytotoxicity, Immunologic , Immunity , Ligands , Mice , Mice, Inbred C57BL , NK Cell Lectin-Like Receptor Subfamily K , Receptors, Natural Killer Cell , Recombinant Proteins , T-Lymphocytes, Cytotoxic/immunology , Tumor Cells, CulturedABSTRACT
Stimulation of natural killer (NK) cells is regulated by a complex balance of inhibitory and stimulatory receptors expressed by NK cells. However, the interaction of stimulatory receptors and their ligands is poorly understood. One stimulatory receptor, NKG2D, is expressed by all NK cells, stimulated CD8+ T cells, gammadelta T cells and macrophages. Recently, progress has been made in defining cellular ligands for NKG2D. Four different families of ligands have been identified in mice and humans, all of which are distantly related to MHC class I molecules. Some of the ligands are upregulated in transformed and infected cells, provoking an attack by the innate and adaptive immune systems. It appears that these "induced-self" ligands recognized by the NKG2D receptor may be a precedent for a new strategy of target cell recognition by the immune system.
Subject(s)
Killer Cells, Natural/immunology , Receptors, Immunologic/metabolism , Animals , CD8-Positive T-Lymphocytes/immunology , Histocompatibility Antigens Class I/metabolism , Humans , Ligands , Macrophages/immunology , Membrane Proteins/metabolism , Mice , Minor Histocompatibility Antigens/metabolism , NK Cell Lectin-Like Receptor Subfamily K , Rats , Receptors, Natural Killer CellABSTRACT
A wide variety of inhibitory and stimulatory NK cell receptors are expressed by some CD8+ cytotoxic T lymphocytes in mice and humans. Recent data address the induction of these receptors on activated or memory CD8+ T cells and have led to hypotheses addressing their function in the CD8+ T cell response.
Subject(s)
Antigens, Ly , CD8-Positive T-Lymphocytes/immunology , Killer Cells, Natural/immunology , Receptors, Immunologic/genetics , Receptors, Immunologic/physiology , Animals , Carrier Proteins/genetics , Carrier Proteins/physiology , Cytotoxicity, Immunologic , Gene Expression , Histocompatibility Antigens Class I/metabolism , Humans , Infections/immunology , Lectins, C-Type , Ligands , Membrane Proteins/genetics , Membrane Proteins/physiology , Mice , Receptors, Immunologic/immunology , Receptors, KIR , Receptors, NK Cell Lectin-Like , Receptors, Natural Killer CellABSTRACT
Two types of T cells, alphabeta and gammadelta, develop in vertebrates. How these two T cell lineages arise from a common thymic T progenitor is poorly understood. Differentiation of alphabeta lineage T cells requires the surrogate alpha chain (pTalpha), which associates with the T cell receptor (TCR) beta chain to form the pre-TCR. gammadelta lineage development does not appear to involve an obligatory surrogate chain, but instead requires productive rearrangement and expression of both TCR gamma and delta genes. It has been proposed that the quality of signals transmitted by the pre-TCR and gammadelta TCR are distinct and that these "instructive" signals determine the lineage fate of an uncommitted progenitor cell. Here we show that the thymic T progenitor cells (CD25(+)CD44(+)c-kit(+)CD3(-)CD4(-)CD8(-) thymocytes, termed pro-T cells) from young adult mice that have yet to express TCRs can be subdivided based on interleukin 7 receptor (IL-7R) expression. These subsets exhibit differential potential to develop into gammadelta versus alphabeta lineage (CD4+CD8+ cells) in the thymus. Upon intrathymic injection, IL-7R(neg-lo) pro-T cells generated a 13-fold higher ratio of alphabeta lineage to gammadelta lineage cells than did IL-7R(+) pro-T cells. Much of this difference was due to a fivefold greater potential of IL-7R(+) pro-T cells to develop into TCR-gammadelta T cells. Evidence indicates that this biased developmental potential is not a result of enhanced TCR-gamma gene rearrangement/expression in IL-7R(+) pro-T cells. These results indicate that the pro-T cells are heterogeneous in developmental potential before TCR gene rearrangement and suggest that in some precursor cells the initial lineage commitment is independent of TCR-mediated signals.
Subject(s)
Hematopoietic Stem Cells/cytology , Receptors, Antigen, T-Cell, alpha-beta , Receptors, Antigen, T-Cell, gamma-delta , Receptors, Interleukin-7/biosynthesis , Signal Transduction/physiology , T-Lymphocytes/cytology , Animals , Cell Differentiation , Cell Lineage , Hematopoietic Stem Cells/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Receptors, Antigen, T-Cell , T-Lymphocytes/metabolismABSTRACT
Natural killer cells express inhibitory receptors specific for MHC class I proteins and stimulatory receptors with diverse specificities. The MHC-specific receptors discriminate among different MHC class I alleles and are expressed in a variegated, overlapping fashion, such that each NK cell expresses several inhibitory and stimulatory receptors. Evidence suggests that individual developing NK cells initiate expression of inhibitory receptor genes in a sequential, cumulative, and stochastic fashion. Superimposed on the receptor acquisition process are multiple education mechanisms, which act to coordinate the stimulatory and inhibitory specificities of developing NK cells. One process influences the complement of receptors expressed by individual NK cells. Other mechanisms may prevent NK cell autoaggression even when the developing NK cell fails to express self-MHC-specific inhibitory receptors. Together, these mechanisms ensure a self-tolerant and maximally discriminating NK cell population. Like NK cells, a fraction of memory phenotype CD8(+) T cells, as well as other T cell subsets, express inhibitory class I--specific receptors in a variegated, overlapping fashion. The characteristics of these cells suggest that inhibitory receptor expression may be a response to prior antigenic stimulation as well as to poorly defined additional signals. A unifying hypothesis is that both NK cells and certain T cell subsets initiate expression of inhibitory receptors in response to stimulation.
Subject(s)
Antigens, Ly , Gene Expression Regulation , Killer Cells, Natural/metabolism , Lectins, C-Type , Receptors, Immunologic/biosynthesis , Animals , Antigens, CD/immunology , Chimera/immunology , Cytotoxicity, Immunologic , Gene Expression Regulation, Developmental , Genes, MHC Class I , Genomic Imprinting , Histocompatibility Antigens Class I/immunology , Histocompatibility Antigens Class II/immunology , Humans , Killer Cells, Natural/classification , Killer Cells, Natural/immunology , Macromolecular Substances , Membrane Glycoproteins/immunology , Mice , Mice, Knockout , Models, Immunological , NK Cell Lectin-Like Receptor Subfamily D , Receptors, Immunologic/genetics , Receptors, Immunologic/immunology , Receptors, KIR , Receptors, NK Cell Lectin-Like , Self Tolerance/immunology , Stochastic Processes , T-Lymphocyte Subsets/immunologyABSTRACT
Inhibitory receptors specific for MHC class I molecules are expressed on partially overlapping subpopulations of NK cells and memory T cells. A central question pertinent to NK cell development and function is how the combinatorial expression of different receptors with distinct class I specificities affects functional recognition. We therefore studied the quantitative effects resulting from class I engagement of multiple inhibitory Ly49 receptors. We used a transgenic mouse model in which all NK cells and T cells express two different Ly49 receptors with shared class I specificity. Comparisons of cells from these mice with cells from single transgenic mice and wild-type mice revealed that Ly49 receptors cumulatively inhibit lymphocyte effector functions. Multiple Ly49 interactions also had a cumulative impact on NK cell development. The findings suggest that the interactions of inhibitory receptors with class I are interpreted quantitatively rather than as on/off switches. They have intriguing implications concerning NK cell tolerance and reactivity toward cells with extinguished expression of a limited number of class I molecules.
Subject(s)
Antigens, Ly , Killer Cells, Natural/immunology , Membrane Glycoproteins/metabolism , Receptors, Immunologic/metabolism , T-Lymphocytes/immunology , Animals , Carrier Proteins/biosynthesis , Carrier Proteins/genetics , Carrier Proteins/physiology , Cells, Cultured , Cytotoxicity, Immunologic/genetics , H-2 Antigens/genetics , H-2 Antigens/immunology , Histocompatibility Antigen H-2D , Immune Tolerance/genetics , Killer Cells, Lymphokine-Activated/immunology , Killer Cells, Lymphokine-Activated/metabolism , Killer Cells, Natural/metabolism , Lectins, C-Type , Lymphocyte Activation/genetics , Lymphocyte Culture Test, Mixed , Membrane Glycoproteins/biosynthesis , Membrane Glycoproteins/genetics , Membrane Glycoproteins/physiology , Membrane Proteins/biosynthesis , Membrane Proteins/genetics , Membrane Proteins/physiology , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Mice, Transgenic , Receptors, Immunologic/biosynthesis , Receptors, Immunologic/genetics , Receptors, NK Cell Lectin-Like , T-Lymphocytes/metabolism , Tumor Cells, Cultured , Wasting Syndrome/genetics , Wasting Syndrome/immunologyABSTRACT
The major histocompatibility complex class Ib protein, Qa-1(b), serves as a ligand for murine CD94/NKG2A natural killer (NK) cell inhibitory receptors. The Qa-1(b) peptide-binding site is predominantly occupied by a single nonameric peptide, Qa-1 determinant modifier (Qdm), derived from the leader sequence of H-2D and L molecules. Five anchor residues were identified in this study by measuring the peptide-binding affinities of substituted Qdm peptides in experiments with purified recombinant Qa-1(b). A candidate peptide-binding motif was determined by sequence analysis of peptides eluted from Qa-1 that had been folded in the presence of random peptide libraries or pools of Qdm derivatives randomized at specific anchor positions. The results indicate that Qa-1(b) can bind a diverse repertoire of peptides but that Qdm has an optimal primary structure for binding Qa-1(b). Flow cytometry experiments with Qa-1(b) tetramers and NK target cell lysis assays demonstrated that CD94/NKG2A discriminates between Qa-1(b) complexes containing peptides with substitutions at nonanchor positions P4, P5, or P8. Our findings suggest that it may be difficult for viruses to generate decoy peptides that mimic Qdm and raise the possibility that competitive replacement of Qdm with other peptides may provide a novel mechanism for activation of NK cells.
Subject(s)
Antigens, CD/metabolism , Histocompatibility Antigens Class I/metabolism , Killer Cells, Natural/immunology , Lectins, C-Type , Membrane Glycoproteins/metabolism , Receptors, Immunologic/metabolism , Animals , Binding Sites , H-2 Antigens/physiology , Histocompatibility Antigen H-2D , Mice , Mice, Inbred C57BL , NK Cell Lectin-Like Receptor Subfamily C , NK Cell Lectin-Like Receptor Subfamily D , Protein Sorting Signals/physiology , Receptors, Natural Killer CellABSTRACT
We report an in vitro stroma-dependent system for the clonal growth and differentiation of natural killer (NK) cells from lymphoid-restricted bone marrow progenitors or bone marrow NK1.1+ cells. Strikingly, the potential to initiate expression of specific Ly49 receptors becomes increasingly restricted as NK cells develop. Moreover, when NK cells express a Ly49 receptor specific for stromal cell class I MHC, they are less likely to initiate expression of another Ly49 receptor in the clonal culture system. The results indicate multiple roles for stromal cells in NK cell development, in supporting clonal growth, in initiation of Ly49 receptor expression, and in formation of the NK cell receptor repertoire.
Subject(s)
Antigens, Ly/metabolism , Histocompatibility Antigens Class I/metabolism , Killer Cells, Natural/metabolism , Membrane Glycoproteins/metabolism , Receptors, Immunologic/metabolism , Animals , Antigens , Antigens, Ly/genetics , Antigens, Surface , Cell Differentiation , Cells, Cultured , Killer Cells, Natural/cytology , Killer Cells, Natural/immunology , Lectins, C-Type , Membrane Glycoproteins/genetics , Membrane Proteins/analysis , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , NK Cell Lectin-Like Receptor Subfamily B , Proteins , Proto-Oncogene Proteins c-kit/analysis , Receptors, Immunologic/biosynthesis , Receptors, Immunologic/genetics , Receptors, NK Cell Lectin-Like , Time Factors , beta 2-Microglobulin/geneticsABSTRACT
Using a new mAb, 2F1, we characterize a mouse natural killer (NK) cell antigen termed 'killer cell lectin-like receptor G1' (KLRG1; formerly mouse MAFA or 2F1-Ag). KLRG1 is expressed on 30-60% of murine NK cells, and a small fraction of T cells, and is composed of a homodimer of glycosylated 30-38-kDa subunits. Strikingly, cell surface expression of KLRG1 by NK cells was substantially down-regulated in mice deficient for expression of class I molecules, in contrast to the Ly49 lectin-like NK receptors, which are up-regulated in class I-deficient mice. We could not demonstrate binding of KLRG1 to class I molecules in a cell-cell adhesion assay. Transgenic expression of KLRG1 under heterologous transcription elements was unaffected by class I deficiency, indicating that class I molecules do not affect the KLRG1 protein directly, and suggesting that regulation is at the level of expression of the endogenous KLRG1 gene. Evidence is presented that class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Thus, although KLRG1 and Ly49 molecules are both lectin-like inhibitory receptors that are regulated by class I expression, the effects of class I on the cell surface expression of the molecules are opposing, and the underlying regulatory mechanisms are distinct.
Subject(s)
Antigens, Ly , Histocompatibility Antigens Class I/metabolism , Killer Cells, Natural/immunology , Receptors, Mitogen/metabolism , Animals , Antibodies, Monoclonal , Chimera , Down-Regulation , Gene Expression , Lectins, C-Type , Membrane Glycoproteins/genetics , Membrane Glycoproteins/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Mutant Strains , Mice, Transgenic , Receptors, Mitogen/chemistry , Receptors, Mitogen/genetics , Receptors, NK Cell Lectin-Like , Signal TransductionABSTRACT
NK1.1+ T cells represent a specialized T cell subset specific for CD1d, a nonclassical MHC class I-restricting element. They are believed to function as regulatory T cells. NK1.1+ T cell development depends on interactions with CD1d molecules presented by hematopoietic cells rather than thymic epithelial cells. NK1.1+ T cells are found in the thymus as well as in peripheral organs such as the liver, spleen, and bone marrow. The site of development of peripheral NK1.1+ T cells is controversial, as is the nature of the CD1d-expressing cell that selects them. With the use of nude mice, thymectomized mice reconstituted with fetal liver cells, and thymus-grafted mice, we provide direct evidence that NK1.1+ T cells in the liver are thymus dependent and can arise in the thymus from fetal liver precursor cells. We show that the class I+ (CD1d+) cell type necessary to select NK1.1+ T cells can originate from TCRalpha-/- precursors but not from TCRbeta-/- precursors, indicating that the selecting cell is a CD4+CD8+ thymocyte. 5-Bromo-2'-deoxyuridine-labeling experiments suggest that the thymic NK1.1+ T cell population arises from proliferating precursor cells, but is a mostly sessile population that turns over very slowly. Since liver NK1.1+ T cells incorporate 5-bromo-2'-deoxyuridine more rapidly than thymic NK1.1+ T cells, it appears that liver NK1.1+ T cells either represent a subset of thymic NK1.1+ T cells or are induced to proliferate after having left the thymus. The results indicate that NK1.1+ T cells, like conventional T cells, arise in the thymus where they are selected by interactions with restricting molecules.
Subject(s)
Antigens/biosynthesis , CD4 Antigens/biosynthesis , CD8 Antigens/biosynthesis , Histocompatibility Antigens Class I/metabolism , Liver/immunology , Protein Biosynthesis , Proteins , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/metabolism , Thymus Gland/immunology , Animals , Antigens, Ly , Antigens, Surface , Bromodeoxyuridine/metabolism , Cell Differentiation/genetics , Cell Differentiation/immunology , Flow Cytometry , Histocompatibility Antigens Class I/genetics , Kinetics , Lectins, C-Type , Liver/cytology , Liver/metabolism , Mice , Mice, Inbred C57BL , Mice, Nude , NK Cell Lectin-Like Receptor Subfamily B , Receptors, Antigen, T-Cell, alpha-beta/deficiency , Receptors, Antigen, T-Cell, alpha-beta/genetics , Receptors, Antigen, T-Cell, alpha-beta/immunology , T-Lymphocyte Subsets/cytology , Thymus Gland/cytology , Thymus Gland/metabolism , beta 2-Microglobulin/deficiency , beta 2-Microglobulin/genetics , beta 2-Microglobulin/immunologyABSTRACT
Natural killer (NK) cells attack tumor and infected cells, but the receptors and ligands that stimulate them are poorly understood. Here we report the expression cloning of two murine ligands for the lectin-like receptor NKG2D. The two ligands, H-60 and Rae1 beta, are distant relatives of major histocompatibility complex class I molecules. NKG2D ligands are not expressed by most normal cells but are up-regulated on numerous tumor cells. We show that mouse NKG2D is expressed by NK cells, activated CD8+ T cells and activated macrophages. Expression of either NKG2D ligand by target cells triggers NK cell cytotoxicity and interferon-gamma secretion by NK cells, as well as nitric oxide release and tumor necrosis factor alpha transcription by macrophages. Thus, through their interaction with NKG2D, H-60 and Rae1 beta are newly identified potent stimulators of innate immunity.
Subject(s)
Killer Cells, Natural/immunology , Macrophages, Peritoneal/immunology , Membrane Proteins/immunology , Minor Histocompatibility Antigens/immunology , Receptors, Immunologic/metabolism , Animals , CHO Cells , COS Cells , Chlorocebus aethiops , Cloning, Molecular , Cricetinae , Humans , Killer Cells, Natural/metabolism , Ligands , Lymphocyte Activation , Macrophage Activation , Macrophages, Peritoneal/metabolism , Membrane Proteins/genetics , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Minor Histocompatibility Antigens/genetics , NK Cell Lectin-Like Receptor Subfamily K , Receptors, Immunologic/genetics , Receptors, Natural Killer Cell , Tumor Cells, CulturedABSTRACT
Natural killer (NK) cells survey potential targets using an array of receptors specific for major histocompatibility complex class I molecules. In mice, members of the Ly49 receptor gene family are expressed on overlapping subsets of NK cells and on CD1-restricted NK1 T cells. Here we characterize a population of memory cytotoxic (CD8(+)) T lymphocytes which also express inhibitory Ly49 family members. This cell population increases steadily with age; by 11 months, over one third of memory CD8(+) T cells express Ly49 molecules. These cells appear to express a normal TCR repertoire, and share several traits with previously activated T cells. Analysis of mutant mouse strains reveals that normal development of these cells depends upon the presence of the transporter associated with antigen presentation (TAP), classical class I molecules, and class II molecules. As a functional consequence of Ly49 expression, we demonstrate that T cell receptor-mediated activation of CD8(+) T cells is inhibited by Ly49 interactions with cognate class I molecules. We hypothesize that conventional memory CD8(+) T cells initiate Ly49 expression as a means of dampening an immune response and / or inhibiting T cell autoreactivity.
Subject(s)
Antigens, Ly , CD8-Positive T-Lymphocytes/chemistry , Histocompatibility Antigens Class I/physiology , Immunologic Memory , Receptors, Immunologic/analysis , Age Factors , Animals , CD8-Positive T-Lymphocytes/immunology , Lectins, C-Type , Lymphocyte Activation , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Receptors, Antigen, T-Cell, alpha-beta/analysis , Receptors, NK Cell Lectin-LikeABSTRACT
The heterodimeric CD94/NKG2A receptor, expressed by mouse natural killer (NK) cells, transduces inhibitory signals upon recognition of its ligand, Qa-1(b), a nonclassical major histocompatibility complex class Ib molecule. Here we clone and express two additional receptors, CD94/NKG2C and CD94/NKG2E, which we show also bind to Qa-1(b). Within their extracellular carbohydrate recognition domains, NKG2C and NKG2E share extensive homology with NKG2A (93-95% amino acid similarity); however, NKG2C/E receptors differ from NKG2A in their cytoplasmic domains (only 33% similarity) and contain features that suggest that CD94/NKG2C and CD94/NKG2E may be activating receptors. We employ a novel blocking anti-NKG2 monoclonal antibody to provide the first direct evidence that CD94/NKG2 molecules are the only Qa-1(b) receptors on NK cells. Molecular analysis reveals that NKG2C and NKG2E messages are extensively alternatively spliced and approximately 20-fold less abundant than NKG2A message in NK cells. The organization of the mouse Cd94/Nkg2 gene cluster, presented here, shows striking similarity with that of the human, arguing that the entire CD94/NKG2 receptor system is relatively primitive in origin. Analysis of synonymous substitution frequencies suggests that within a species, NKG2 genes may maintain similarities with each other by concerted evolution, possibly involving gene conversion-like events. These findings have implications for understanding NK cells and also raise new possibilities for the role of Qa-1 in immune responses.
Subject(s)
Antigens, CD/physiology , Histocompatibility Antigens Class I/physiology , Killer Cells, Natural/physiology , Lectins, C-Type , Membrane Glycoproteins/physiology , Receptors, Immunologic/physiology , Amino Acid Sequence , Animals , CHO Cells , Cloning, Molecular , Cricetinae , Humans , Ligands , Mice , Molecular Sequence Data , NK Cell Lectin-Like Receptor Subfamily C , NK Cell Lectin-Like Receptor Subfamily D , Receptors, Natural Killer Cell , Sequence AlignmentABSTRACT
Inhibitory receptors specific for alleles of MHC class I proteins play an important role in determining the reactivity and specificity of NK cells. To determine whether these receptors are also able to regulate T cell functions, we have studied anti-viral immune responses in mice transgenic for a class I-specific inhibitory receptor, Ly49A. Although nontransgenic mice express Ly49A primarily on NK cells and some T cells, the Ly49A transgenic mice express Ly49A on all lymphocytes, including T cells. We have assessed the activation, expansion, cytokine production, and cytotoxic activity of CD8 T cells in both transgenic and nontransgenic mice following infection with lymphocytic choriomeningitis virus. As expected, nontransgenic mice made a potent virus-specific CD8 T cell response following virus infection. However, as measured in cytolysis assays and by cytokine production, virus-specific CD8 T cell activity was reduced in Ly49A transgenic mice. This inhibition was largely, but not always exclusively, dependent upon the presence, either in vivo or in vitro, of the Ly49A ligand, H-2Dd. Strikingly Ly49A transgenic mice have reduced capacity to control infection with the virulent lymphocytic choriomeningitis virus variant clone 13. Overall, these studies demonstrate that expression of killer inhibitory receptors can modulate anti-viral T cell responses in vivo and in vitro.
Subject(s)
Antigens, Ly , Carrier Proteins/biosynthesis , Lymphocytic choriomeningitis virus/immunology , Membrane Proteins/biosynthesis , Receptors, Immunologic/biosynthesis , T-Lymphocytes/immunology , T-Lymphocytes/virology , Animals , CD8-Positive T-Lymphocytes/cytology , CD8-Positive T-Lymphocytes/immunology , Cell Division/immunology , Cytokines/biosynthesis , H-2 Antigens/genetics , Histocompatibility Antigen H-2D , Lectins, C-Type , Lymphocyte Activation , Lymphocytic Choriomeningitis/genetics , Lymphocytic Choriomeningitis/immunology , Lymphocytic Choriomeningitis/virology , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Transgenic , NK Cell Lectin-Like Receptor Subfamily A , Receptors, Antigen, T-Cell, alpha-beta/biosynthesis , Receptors, Antigen, T-Cell, alpha-beta/genetics , Receptors, NK Cell Lectin-Like , T-Lymphocytes/metabolism , T-Lymphocytes, Cytotoxic/immunology , T-Lymphocytes, Cytotoxic/virologyABSTRACT
Natural killer cells attack tumor cells, infected cells and some normal cells, but the basis of their specificity is not completely understood. Recent studies indicate that epithelial tumor cells upregulate a stress-induced MHC class-I-like protein termed MICA, triggering NK cells via a recently described receptor called NKG2D.
Subject(s)
Carcinoma/immunology , Heat-Shock Proteins/immunology , Histocompatibility Antigens Class I/immunology , Killer Cells, Natural/immunology , Membrane Proteins/immunology , Models, Immunological , Neoplasm Proteins/immunology , Receptors, Immunologic/immunology , Animals , Humans , Lymphocyte Activation , Membrane Proteins/chemistry , Mice , NK Cell Lectin-Like Receptor Subfamily K , Receptors, Immunologic/chemistry , Receptors, Natural Killer Cell , T-Lymphocytes/immunologyABSTRACT
We produced a novel hamster monoclonal antibody (MAb), 14B11, that recognizes the majority of mouse natural-killer (NK) cells. Transfection studies demonstrated that 14B11 MAb binds a subset of Ly49 receptors, including three putative inhibitory receptors, Ly49F, I, and C. No binding to Ly49A, B, D, or G was detected. In addition, 14B11 was shown to bind the putative activating receptor Ly49H, which required co-transfection of the signaling molecule DAP12 for detectable cell surface expression. Thus, 14B11 is the first reported MAb to bind Ly49H and F. At the functional level, 14B11 MAb enhanced the lysis by IL-2 activated NK cells of an FcR+ target cell line (Daudi), but not an FcR- target cell (EL-4). Because F(ab')2 fragments of 14B11 failed to enhance lytic activity, the enhancement of lysis by intact antibody is apparently due to "redirected lysis," in which stimulatory receptors on the NK cell are bridged by antibody to Fc receptors on the target cell. Cell separation experiments demonstrated that the 14B11-dependent redirected lysis was markedly increased using NK cell populations that had been depleted of Ly49F,+ I,+ or C+ NK cells. Because such depletions are expected to enrich for Ly49H+ NK cells, these results suggest that the enhancement of lysis mediated by 14B11 MAb may be due to stimulation of the activating Ly49H receptor. In conjunction with other anti-Ly49 MAbs, the 14B11 MAb will be useful in further studies of Ly49 receptor function and specificity.
Subject(s)
Antibodies, Monoclonal/biosynthesis , Antibodies, Monoclonal/metabolism , Cytotoxicity, Immunologic , Killer Cells, Natural/chemistry , Killer Cells, Natural/immunology , Receptors, Immunologic/immunology , Animals , Antibodies, Monoclonal/immunology , Antibody Specificity , Antigens/analysis , COS Cells , Cricetinae , Killer Cells, Lymphokine-Activated/chemistry , Killer Cells, Lymphokine-Activated/immunology , Lymphocyte Subsets/immunology , Mesocricetus , Mice , Mice, Inbred BALB C , Mice, Inbred C3H , Mice, Inbred C57BL , Mice, Inbred Strains , Receptors, Immunologic/analysis , Species Specificity , Tumor Cells, CulturedABSTRACT
Mice lacking the interleukin 7 receptor (IL-7R) generate alpha/beta T cells at a detectable but greatly reduced rate, but gamma/delta T cells are completely absent. The special role of IL-7R signaling in gamma/delta T cell development has remained unclear. IL-7Ralpha(-/-) mice exhibit a paucity of gamma gene rearrangements. This striking observation can be explained by a defect in T cell receptor (TCR)-gamma gene rearrangement, a defect in TCR-gamma gene transcription leading to death of gamma/delta lineage cells, and/or a requirement for IL-7R in commitment of cells to the gamma/delta lineage. To determine the role of IL-7R signaling in gamma/delta T cell development, we examined transcription of a prerearranged TCR-gamma transgene in IL-7Ralpha(-/-) mice, as well as the effects of IL-7 on transcription of endogenous, rearranged TCR-gamma genes in alpha/beta lineage cells. The results demonstrate that IL-7R-mediated signals are necessary for the normal expression of rearranged TCR-gamma genes. Equally significant, the results show that the poor expression of TCR-gamma genes in IL-7Ralpha(-/-) mice is responsible for the selective deficit in gamma/delta cells in these mice, since a high copy TCR-gamma transgene exhibited sufficient residual expression in IL-7Ralpha(-/-) mice to drive gamma/delta cell development. The results indicate that the absence of gamma/delta T cells in IL-7Ralpha(-/-) mice is due to insufficient TCR-gamma gene expression.