1.
J Clin Microbiol
; 39(10): 3709-11, 2001 Oct.
Article
in English
| MEDLINE
| ID: mdl-11574598
ABSTRACT
Restriction fragment length polymorphism (RFLP) analysis of IS6110 is commonly used to DNA fingerprint Mycobacterium tuberculosis. However, low-copy (< or =5) IS6110 M. tuberculosis strains are poorly differentiated, requiring secondary typing. When spoligotyping was used as the secondary method, only 13% of Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered strains had epidemiologic linkages to another patient, compared to 48% of those with high-copy strains clustered by IS6110 alone (P < 0.01). Spoligotyping did not improve a population-based molecular epidemiologic study of recent TB transmission.
Subject(s)
DNA Fingerprinting , DNA Transposable Elements/genetics , Gene Dosage , Mycobacterium tuberculosis/classification , Tuberculosis, Pulmonary/epidemiology , Aged , Bacterial Typing Techniques , Humans , Mycobacterium tuberculosis/genetics , Oligonucleotides/analysis , Polymorphism, Restriction Fragment Length , Tuberculosis, Pulmonary/microbiology
2.
J Clin Microbiol
; 38(11): 4300, 2000 Nov.
Article
in English
| MEDLINE
| ID: mdl-11185066
3.
Emerg Infect Dis
; 5(6): 838-9, 1999.
Article
in English
| MEDLINE
| ID: mdl-10603227
4.
Clin Infect Dis
; 29(3): 700, 1999 Sep.
Article
in English
| MEDLINE
| ID: mdl-10530482