ABSTRACT
Quantum memories are vital to the scalability of photonic quantum information processing (PQIP), since the storage of photons enables repeat-until-success strategies. On the other hand, the key element of all PQIP architectures is the beam splitter, which allows us to coherently couple optical modes. Here, we show how to combine these crucial functionalities by addressing a Raman quantum memory with multiple control pulses. The result is a coherent optical storage device with an extremely large time bandwidth product, that functions as an array of dynamically configurable beam splitters, and that can be read out with arbitrarily high efficiency. Networks of such devices would allow fully scalable PQIP, with applications in quantum computation, long distance quantum communications and quantum metrology.
ABSTRACT
Quantum entanglement in the motion of macroscopic solid bodies has implications both for quantum technologies and foundational studies of the boundary between the quantum and classical worlds. Entanglement is usually fragile in room-temperature solids, owing to strong interactions both internally and with the noisy environment. We generated motional entanglement between vibrational states of two spatially separated, millimeter-sized diamonds at room temperature. By measuring strong nonclassical correlations between Raman-scattered photons, we showed that the quantum state of the diamonds has positive concurrence with 98% probability. Our results show that entanglement can persist in the classical context of moving macroscopic solids in ambient conditions.
ABSTRACT
Room-temperature, easy-to-operate quantum memories are essential building blocks for future long distance quantum information networks operating on an intercontinental scale, because devices like quantum repeaters, based on quantum memories, will have to be deployed in potentially remote, inaccessible locations. Here we demonstrate controllable, broadband and efficient storage and retrieval of weak coherent light pulses at the single-photon level in warm atomic cesium vapor using the robust far off-resonant Raman memory scheme. We show that the unconditional noise floor of this technically simple quantum memory is low enough to operate in the quantum regime, even in a room-temperature environment.
ABSTRACT
Schizophrenia is a devastating disease that affects approximately 1% of the population across cultures. Its neurobiological underpinnings are still unknown. Accordingly, animal models of schizophrenia often lack construct validity. As concordance rate in monozygotic twins amounts to only 50%, environmental risk factors (e.g. neurotrauma, drug abuse, psychotrauma) likely act as necessary 'second hit' to trigger/drive the disease process in a genetically predisposed individual. Valid animal models would have to consider this genetic-environmental interaction. Based on this concept, we designed an experimental approach for modeling a schizophrenia-like phenotype in mice. As dysfunction in synaptic transmission plays a key role in schizophrenia, and complexin2 (CPLX2) gene expression is reduced in hippocampus of schizophrenic patients, we developed a mouse model with Cplx2 null mutation as genetic risk factor and a mild parietal neurotrauma, applied during puberty, as environmental 'second hit'. Several months after lesion, Cplx2 null mutants showed reduced pre-pulse inhibition, deficit of spatial learning and loss of inhibition after MK-801 challenge. These abnormalities were largely absent in lesioned wild-type mice and non-lesioned Cplx2 null mutants. Forced alternation in T-maze, object recognition, social interaction and elevated plus maze tests were unaltered in all groups. The previously reported mild motor phenotype of Cplx2 null mutants was accentuated upon lesion. MRI volumetrical analysis showed a decrease of hippocampal volume exclusively in lesioned Cplx2 null mutants. These findings provide suggestive evidence for the 'second hit' hypothesis of schizophrenia and may offer new tools for the development of advanced treatment strategies.
Subject(s)
Adaptor Proteins, Vesicular Transport/deficiency , Adaptor Proteins, Vesicular Transport/genetics , Nerve Tissue Proteins/deficiency , Nerve Tissue Proteins/genetics , Parietal Lobe/injuries , Schizophrenia/etiology , Schizophrenia/genetics , Animals , Disease Models, Animal , Dizocilpine Maleate/pharmacology , Humans , Male , Maze Learning/physiology , Mice , Mice, Inbred C57BL , Mice, Knockout , Phenotype , Recognition, Psychology/physiology , Reflex, Startle/physiology , Risk Factors , Social Behavior , Space Perception/physiologyABSTRACT
The short-term dynamics of synaptic communication between neurons provides neural networks with specific frequency-filter characteristics for information transfer. The direction of short-term synaptic plasticity, that is, facilitation versus depression, is highly dependent on and inversely correlated to the basal release probability of a synapse. Amongst the processes implicated in shaping the release probability, proteins that regulate the docking and priming of synaptic vesicles at the active zone are of special importance. Here, we found that a member of the Munc13 protein family of priming proteins, namely Munc13-2, is essential for normal release probability at hippocampal mossy fiber synapses. Paired pulse and frequency facilitation were strongly increased, whereas mossy fiber long-term potentiation was unaffected in the absence of Munc13-2. In contrast, transmission at 3 other types of hippocampal synapses, Schaffer-collateral, associational-commissural, as well as inhibitory synapses onto CA3 pyramidal neurons was unaffected by the loss of Munc13-2.
Subject(s)
Hippocampus/cytology , Intracellular Signaling Peptides and Proteins/metabolism , Nerve Tissue Proteins/metabolism , Neuronal Plasticity/genetics , Neuronal Plasticity/physiology , Synapses/physiology , Animals , Animals, Newborn , Calcium/metabolism , Cyclopropanes/pharmacology , Dipeptides/pharmacology , Electric Stimulation/methods , Excitatory Amino Acid Antagonists/pharmacology , Excitatory Postsynaptic Potentials/drug effects , Excitatory Postsynaptic Potentials/genetics , GABA Antagonists/pharmacology , Glycine/analogs & derivatives , Glycine/pharmacology , In Vitro Techniques , Intracellular Signaling Peptides and Proteins/deficiency , Long-Term Potentiation/drug effects , Long-Term Potentiation/genetics , Mice , Mice, Knockout , Mossy Fibers, Hippocampal/drug effects , Mossy Fibers, Hippocampal/physiology , Nerve Tissue Proteins/deficiency , Neuronal Plasticity/drug effects , Patch-Clamp Techniques/methods , Pyridazines/pharmacology , Quinoxalines/pharmacology , Synapses/drug effectsABSTRACT
The ability to store multiple optical modes in a quantum memory allows for increased efficiency of quantum communication and computation. Here we compute the multimode capacity of a variety of quantum memory protocols based on light storage in ensembles of atoms. We find that adding a controlled inhomogeneous broadening improves this capacity significantly.
ABSTRACT
Detailed measurements of spontaneous magnetic reconnection are presented. The experimental data, which were obtained in the new closed Versatile Toroidal Facility magnetic configuration, document the profile evolution of the plasma density, magnetic flux function, reconnection rate, and the current density during a spontaneous reconnection event in the presence of a strong guide magnetic field. The reconnection process is at first slow, which allows magnetic stress to build in the system while the current channel becomes increasingly narrow and intense. The onset of a fast reconnection event occurs as the width of the current channel approaches the ion-sound-Larmor radius rho s. During the reconnection event magnetically stored energy is channeled into energetic ion outflows and a rapid increase in the electron temperature.
ABSTRACT
The testis-specific proacrosin gene encodes for a fertilization-promoting protein. In mouse and rat it is first transcribed in late pachytene spermatocytes and revealed to be translationally regulated. Former proacrosin promoter studies demonstrated that elements necessary for conducting a stage and temporal-specific expression of the gene are located within 0.9 kb upstream of the translational start codon. In the present study we analyzed putative cis-acting elements located in this promoter region for their specific binding properties to nuclear factors assumed to be involved in proacrosin gene regulation. Supplement of specific antibodies in electrophoretic mobility shift assays (EMSA) revealed that two Y-box proteins and the transcription factors CREM and YY1 interact with proacrosin promoter elements. The Y-box proteins, antigenically related to the frog Y-box proteins FRGY1 and FRGY2, bound to the Y-box (55-66 bp upstream of the ATG initiation codon) in brain and testis nuclear extracts, respectively. CREM bound to three elements (30-37, 252-259, and 717-724 bp upstream of ATG). The ubiquitous transcription factor YY1 bound to a conserved element in the central proacrosin promoter (457-473 bp upstream of ATG) and showed almost germ cell-specific truncates in EMSA. These results suggest that the identified factors are involved in proacrosin gene regulation.
Subject(s)
Acrosin/genetics , Enzyme Precursors/genetics , Germ Cells/metabolism , Promoter Regions, Genetic/genetics , Repressor Proteins , Animals , Base Sequence , Binding Sites , Cyclic AMP Response Element Modulator , DNA/genetics , DNA/metabolism , DNA Footprinting , DNA-Binding Proteins/metabolism , Electrophoresis, Polyacrylamide Gel , Erythroid-Specific DNA-Binding Factors , Mice , Molecular Sequence Data , Nuclear Proteins/metabolism , Organ Specificity , Rats , Response Elements/genetics , Transcription Factors/metabolism , YY1 Transcription FactorABSTRACT
Synaptic vesicle fusion at synapses is triggered by increases in cytosolic Ca2+ levels. However, the identity of the Ca2+ sensor and the transduction mechanism of the Ca2+ trigger are unknown. We show that Complexins, stoichiometric components of the exocytotic core complex, are important regulators of transmitter release at a step immediately preceding vesicle fusion. Neurons lacking Complexins show a dramatically reduced transmitter release efficiency due to decreased Ca2+ sensitivity of the synaptic secretion process. Analyses of mutant neurons demonstrate that Complexins are acting at or following the Ca2+-triggering step of fast synchronous transmitter release by regulating the exocytotic Ca2+ sensor, its interaction with the core complex fusion machinery, or the efficiency of the fusion apparatus itself.
Subject(s)
Calcium/metabolism , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/metabolism , Neurotransmitter Agents/metabolism , Synaptic Transmission/physiology , Adaptor Proteins, Vesicular Transport , Animals , Calcimycin/pharmacology , Cells, Cultured , Excitatory Postsynaptic Potentials/drug effects , Excitatory Postsynaptic Potentials/physiology , Gene Deletion , Hippocampus/cytology , Ionophores/pharmacology , Mice , Mice, Mutant Strains , Microscopy, Electron , Neuronal Plasticity/physiology , Neurons/physiology , Neurons/ultrastructure , Patch-Clamp Techniques , Synaptic Transmission/drug effects , Synaptic Vesicles/physiologyABSTRACT
The synthesis of transition protein (TNP) 2, one of the predominant nuclear proteins of mammalian spermatids, was shown to be posttranscriptionally regulated, by storing the untranslated mRNA for about 3-5 days in the cytoplasm of differentiating spermatids. It has been proposed that binding of a cytoplasmic protein to a conserved motif of 8 nucleotides (nt) in the 3' untranslated region (3'UTR) of TNP2 mRNA is involved in this translational control mechanism. In this report, we show that deletion or variation of the conserved 8-nt motif (GCCAT-CAC) in rat TNP2-3'UTR abolishes the capacity of the in vitro-transcribed RNA to reconstitute specific RNA-protein complexes in RNA bandshift assays. Using Northwestern analysis, we identified specific binding to the TNP2-3'UTR of four proteins of 45, 47, 49, and 60 kDa, all of which are stage-specifically regulated in male germ cell differentiation. Deletion of the 8-nt motif in the 3'UTR specifically prevented binding of the 47-kDa protein, the interaction of which is thought to be mediated by the RNA secondary structure. Analysis of the RNA secondary structure revealed that the 8-nt motif is an essential element of a specific stem-loop structure that is predicted for rat wild-type TNP2-3'UTR. Therefore we assume that the 47-kDa protein plays an important role in specific RNA-protein complex formation of rat TNP2-3'UTR that may be a central event in the translational control of rat TNP2 mRNA.
Subject(s)
Nuclear Proteins/biosynthesis , Protein Processing, Post-Translational/physiology , RNA, Messenger/metabolism , RNA-Binding Proteins/physiology , Ammonium Sulfate/chemistry , Animals , Blotting, Northern , Blotting, Western , Chromosomal Proteins, Non-Histone/biosynthesis , Cytoplasm/metabolism , DNA-Binding Proteins , Germ Cells/physiology , Male , Molecular Weight , Nucleic Acid Conformation , Protein Binding , RNA-Binding Proteins/metabolism , Rats , Transcription, Genetic/physiologyABSTRACT
Spermatogenesis is a complex developmental process which involves amplification of germinal stem cells, their differentiation into spermatocytes, meiotic division and finally transformation into mature spermatozoa. Therefore, spermatogenesis provides an interesting system for examining the regulation of gene expression during development and differentiation. The genes expressed during spermatogenesis can be divided into two main groups: diploid and haploid expressed genes. In this review, we report about the regulation of expression of a diploid expressed gene, namely the proacrosin gene, and that of a haploid expressed gene, the transition protein 2 gene.
Subject(s)
Gene Expression Regulation, Developmental , Spermatogenesis/genetics , Acrosin/genetics , Animals , Base Sequence , Chromosomal Proteins, Non-Histone/genetics , DNA, Complementary/genetics , Diploidy , Enzyme Precursors/genetics , Haploidy , Humans , Male , Protamines/genetics , Protein BiosynthesisABSTRACT
Acrosin, a sperm acrosomal serine protease has been implicated in the recognition, binding and penetration of the zona pellucida of the ovum. Biosynthesis of acrosin was found to start in early round spermatids which are haploid germ cells. Here, we report that acrosin gene transcription occurs as early as at day 19 of rat spermatogenesis which contains diploid but not haploid spermatogenic cells. Translational control of the acrosin gene may be due to cytoplasmic protein factors which through RNA-bandshift experiments were found to bind to the 5'UTR of the acrosin mRNA. In order to differentiate between diploid and haploid spermatogenic cells at the molecular level, transcription of the protamine 2 gene during rat testicular development was evaluated. Protamine 2 transcripts could be demonstrated for the first time in 25-day-old testes which contain diploid as well as haploid spermatogenic cells.