Effects of two protocols of ovulation synchronization on corpus luteum size and blood flow, progesterone concentration, and pregnancy rate in beef heifers.

The objective of this study was to evaluate the effect of 7-day estradiol-progesterone-based [Treat(C)] and 5-day Co-Synch plus progesterone [Treat(Co-Sy)] protocols on ovulation time, pre-ovulatory follicle diameter, corpus luteum (CL) size and blood flow, progesterone (P4) concentration and pregnancy rate (PR) in beef heifers. In Experiment 1, a crossover design was applied (n = 9). For Treat(C), a progesterone intravaginal (PI) device was inserted, plus 2 mg of estradiol benzoate (day 0). On day 7, 500 µg of cloprostenol plus 0.5 mg of estradiol cypionate were administered, and PI was removed. For Treat(Co-Sy), on day 0, a PI was inserted plus 100 µg gonadotropin-releasing hormone (GnRH). On day 5, PI was removed, plus 500 µg of cloprostenol and 100 µg of GnRH were administered at 69-70 h later. From day one to ovulation day, dominant follicle was evaluated by ultrasonography. On days 4 and 8 post-ovulation, CL was evaluated by color Doppler, and P4 concentration was determined by chemiluminescence. In Experiment 2, a split-plot experimental design was used. Protocols followed were the same as in Experiment 1 [Treat(C); n = 310 and Treat(Co-Sy); n = 314]. Heifers were fixed-time artificially inseminated. Pregnancy was determined on day 41. In Experiment 1, the interval between PI removal and ovulation time was different between protocols (P < 0.01). In addition, P4 concentration was related to the CL size (P < 0.001), CL blood flow (P < 0.01) and protocols (P < 0.03). In Experiment 2, PR did not differ between protocols.

Follicular wave dynamics and Growth factors gene expression in Braford heifers.

The objectives of the present study were (experiment 1) to characterized development and dynamics of the dominant follicles (DF) and the corpus luteum (CL) to determine patterns of two (W2) and three (W3) follicular waves in beef heifers, and (experiment 2) to determine gene expression of growth factors gene expression in follicular cells of W2 and W3 heifer. Twenty-eight Braford heifers were used. Dominant follicular and CL were monitored daily by ultrasonography to identify the development W2 and W3 in heifers. Pre-ovulatory DF were aspirated on day 19 in W2 and on day 22 in W3 heifers. In W2 and W3, follicular cells (FC) of gene expression of growth differentiation factor 9, bone morphogenetic protein 15 (BMP15), fibroblast growth factor basic, transforming growth factor beta receptor 1, bone morphogenetic protein receptor type IB and fibroblast growth factor receptor 2 were evaluated. The regression of the DF of the first follicular wave and the emergency of the DF of the second follicular wave began later in the heifers W2 than in W3 (p = .02 and p < .01). The regression of the CL began earlier in the W2 than in W3 group (p < .01). Gene expression of growth factors and receptors was similar between groups. However, higher relative levels of BMP15 was observed in W2 group (p = .07). Results propose that wave patterns were regulated by the development time of the DF in the first wave and the life of the CL. Furthermore, higher levels of BMP15 could produce shorter life of CL. The present work suggest that ultrasonography associated with molecular assays could be used as an easy and effective tool to characterize follicular wave patterns.

Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers.

OBJECTIVE: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. METHODS: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. RESULTS: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). CONCLUSION: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.

Hormones and monensin use to improve pregnancy rates in grazing lactating beef cows in the semiarid region of Argentina.

The objectives of this study were 1) to determine the effect of monensin treatment, alone or combined with a hormonal estrus synchronization treatment, on the pregnancy rate of lactating beef cows, and 2) to evaluate the effect of monensin capsule administration on ruminal metabolism in steers. In experiment 1, ninety-four cows were selected from a 300 cow herd. The experimental design used was a 2 x 2 factorial with the administration of monensin capsule as first factor (M1 = with monensin vs. M0 = without monensin) and hormonal treatment as second factor (H1 = with hormonal treatment vs. H0 = no hormonal treatment). Thirty-eight days before the beginning of the breeding season, cows were randomly assigned to the first factor, and thirty days later to the second factor, resulting in four treatments: M1H0, M1H1, M0H0 and M0H1. Cow were exposed to bull (bull/cow ratio 1:20) from day 0 (day 0 = start of the breeding season and 38 days after monensin capsule administration) to day 50. Pregnancy diagnosis was performed at 30, 60 and 80 days after start breeding season by ultrasonography. In experiment 2, eight ruminally cannulated crossbred beef steers were randomly assigned to two treatments (M1 and M0). To determine proportion of volatile fatty acids (VFA), ruminal fluid samples were taken on days 0, 40 and 77 of the experimental period, at 0, 4 and 12 h after grazing. In experiment 1, treatments whit monensin did not improve pregnancy rate (P = 0.95), however, hormonal treatment resulted in grater pregnancy rates (P = 0.03). In experiment 2, the proportion of VFA in ruminal fluid of steers was significantly different between treatments. The highest proportion of propionate was found in ruminal fluid from M1 treatment at 12 h after grazing (P = 0.04). In conclusion, the treatment with monensin increased the proportion of propionate. The result might suggest that energy balance was improved in steers, without improvement in cow´s pregnancy rates. Treatment with monensin alone did not improve pregnancy rate, nor did treatment with monensin enhance the pregnancy rate when a hormonal synchronisation treatment was given. Nevertheless, the use of a hormonal treatment increased pregnancy rate, suggesting that it could be used as a suitable tool to enhance the productivity in cows with marginal body condition score.