Cobalt complexes modulate plasmid conjugation in Escherichia coli and Klebsiella pneumoniae.

Antimicrobial resistance genes (ARG), such as extended-spectrum ß-lactamase (ESBL) and carbapenemase genes, are commonly carried on plasmids. Plasmids can transmit between bacteria, disseminate globally, and cause clinically important resistance. Therefore, targeting plasmids could reduce ARG prevalence, and restore the efficacy of existing antibiotics. Cobalt complexes possess diverse biological activities, including antimicrobial and anticancer properties. However, their effect on plasmid conjugation has not been explored yet. Here, we assessed the effect of four previously characterised bis(N-picolinamido)cobalt(II) complexes lacking antibacterial activity on plasmid conjugation in Escherichia coli and Klebsiella pneumoniae. Antimicrobial susceptibility testing of these cobalt complexes confirmed the lack of antibacterial activity in E. coli and K. pneumoniae. Liquid broth and solid agar conjugation assays were used to screen the activity of the complexes on four archetypical plasmids in E. coli J53. The cobalt complexes significantly reduced the conjugation of RP4, R6K, and R388 plasmids, but not pKM101, on solid agar in E. coli J53. Owing to their promising activity, the impact of cobalt complexes was tested on the conjugation of fluorescently tagged extended-spectrum ß-lactamase encoding pCTgfp plasmid in E. coli and carbapenemase encoding pKpQILgfp plasmid in K. pneumoniae, using flow cytometry. The complexes significantly reduced the conjugation of pKpQILgfp in K. pneumoniae but had no impact on pCTgfp conjugation in E. coli. The cobalt complexes did not have plasmid-curing activity, suggesting that they target conjugation rather than plasmid stability. To our knowledge, this is the first study to report reduced conjugation of clinically relevant plasmids with cobalt complexes. These cobalt complexes are not cytotoxic towards mammalian cells and are not antibacterial, therefore they could be optimised and employed as inhibitors of plasmid conjugation.

Tuning the Aggregates of Thiophene-based Trimers by Methyl Side-chain Engineering for Photocatalytic Hydrogen Evolution.

Organic π-conjugated semiconductors (OCSs) have recently emerged as a promising alternative to traditional inorganic materials for photocatalysis. However, the aggregation of OCSs in photocatalytic aqueous solution caused by self-assembly, which closely relates to the photocatalytic activity, has not yet been studied. Here, the relationship between the aggregation of 4,7-Bis(thiophen-2-yl) benzothiadiazole (TBT) and the photocatalytic activity was systematically investigated by introducing and varying the position of methyl side chains on the two peripheral thiophene units. Experimental and theoretical results indicated that the introduction of -CH3 group at the 3-position of TBT resulted in the smallest size and best crystallinity of aggregates compared to that of TBT, 4- and 5-positions. As a result, TBT-3 exhibited an excellent photocatalytic activity towards H2 evolution, ascribed to the shorten charge carrier transport distance and solid long-range order. These results suggest the important role of aggregation behavior of OCSs for efficient photocatalysis.

Analysing the impact of eriosematin E from Eriosema chinense Vogel. against different diarrhoeagenic pathovars of Escherichia coli using in silico and in vitro approach.

Since diarrhoea is reportedly the third largest cause of fatality among kids, therefore it is considered to be one of the major areas of concerns among developing nations. The main causative agents of diarrhoea include Escherichia coli, Vibrio cholera, and Shigella spp where E. coli shares the maximum contribution. The roots of the plant Eriosema chinense Vogel. (Fabaceae) are traditionally used by the native tribes of Meghalaya, India to treat diarrhoea. From previous reports, the plant and its marker eriosematin E have been reported to have antidiarrhoeal potential against pathogenic and nonpathogenic diarrhoea. Therefore, the objective of the current investigation was to use in silico studies to determine the efficacy of eriosematin E against different diarrhoeagenic strains of E. coli. Six different pathovars of E. coli i.e. enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enterohaemorrhagic E. coli (EHEC), enteroaggregative E. coli (EAEC), uropathogenic E. coli (UPEC) and enteroinvasive E. coli (EIEC) were subjected to docking simulation studies utilizing Glide module of Schrodinger Maestro 2018-1 MM Share Version. Based on the obtained binding energy and balance between H-bonding, hydrophobic, and salt bridge interactions eriosematin E was found to be most effective against EPEC followed by EAEC and ETEC, while UPEC and EHEC were moderately affected. The molecular dynamics studies suggested a higher affinity of eriosematin E towards heat-labile enterotoxin b-pentamer from ETEC. The in vitro antibacterial studies against the universal strain S. aureus 12981 and E. coli 10418 revealed the effectiveness of eriosematin E showing MIC values of ≥256 µg/mL.Communicated by Ramaswamy H. Sarma.

The epistemologies of research on the survival of consciousness after death in the golden era of the Society for Psychical Research (1882-1930).

The Society for Psychical Research (SPR) of London was founded in 1882 with the purpose of investigating psychical phenomena, especially the theme of survival, with scientific rigour. Despite the recognized importance of the SPR for dynamic psychiatry in the nineteenth and twentieth centuries, there are few studies of its epistemological contributions to the theme of survival and its implications to science. In order to fill this gap, we have consulted the main journals of the SPR in its golden period, and highlight the epistemologies of Sidgwick, Myers, James, Podmore, Schiller, Lodge and Richet. We conclude that the authors, whether for or against survival, argued in defence of an expanded science, and looked forward to understanding the complexity of human experience.

LY2183240 regioisomers act as competitive and selective inhibitors of class C ß-lactamases.

The regioisomers of the anandamide-acting drug LY2183240 exhibited specific potent and competitive inhibitory activities against class C ß-lactamases. More explicitly, the 1,5- and 2,5-regioisomers inhibited AmpC from Enterobacter hormaechei (formerly Enterobacter cloacae) with inhibitor binding affinity values of 1.8 µM and 2.45 µM, respectively. Structural molecular modelling studies revealed the interaction of the regioisomers with the relevant residues of the catalytic site of cephalosporinase from E. hormaechei P99, which included Tyr150, Lys315 and Thr316.

Antimicrobial and anthelmintic activities of aryl urea agents.

OBJECTIVES: This study aimed to characterise compounds with activity against carbapenemase-expressing Gram-negative bacteria and nematodes and evaluate their cytotoxicity to non-cancerous human cells. METHODS: The antimicrobial activity and toxicity of a series of phenyl-substituted urea derivatives were evaluated using broth microdilution, chitinase, and resazurin reduction assays. RESULTS: The effects of different substitutions present on the nitrogen atoms of the urea backbone were investigated. Several compounds were active against Staphylococcus aureus and Escherichia coli control strains. Specifically, derivatives 7b, 11b, and 67d exhibited antimicrobial activity against Klebsiella pneumoniae 16, a carbapenemase-producing Enterobacteriaceae species, with minimum inhibitory concentration (MIC) values of 100, 50, and 72 µM (32, 64, and 32 mg/L), respectively. In addition, the MICs obtained against a multidrug-resistant E. coli strain were 100, 50, and 36 µM (32, 16, and 16 mg/L) for the same compounds, respectively. Furthermore, the urea derivatives 18b, 29b, 50c, 51c, 52c, 55c-59c, and 62c were very active towards the nematode Caenorhabditis elegans. CONCLUSIONS: Testing on non-cancerous human cell lines suggested that some of the compounds have the potential to affect bacteria, especially helminths, with limited cytotoxicity to humans. Given the simplicity of synthesis for this class of compounds and their potency against Gram-negative, carbapenemase-expressing K. pneumoniae, aryl ureas possessing the 3,5-dichloro-phenyl group certainly warrant further investigation to exploit their selectivity.

The Thermoelectric Properties of Spongy PEDOT Films and 3D-Nanonetworks by Electropolymerization.

Recently, polymers have been attracted great attention because of their thermoelectric materials' excellent mechanical properties, specifically their cost-effectiveness and scalability at the industrial level. In this study, the electropolymerization conditions (applied potential and deposition time) of PEDOT films were investigated to improve their thermoelectric properties. The morphology and Raman spectroscopy of the PEDOT films were analyzed according to their applied potential and deposition time. The best thermoelectric properties were found in films grown at 1.3 V for 10 min, with an electrical conductivity of 158 ± 8 S/cm, a Seebeck coefficient of 33 ± 1 µV/K, and a power factor of 17 ± 2 µW/K·m2. This power factor value is three times higher than the value reported in the literature for electropolymerized PEDOT films in acetonitrile using lithium perchlorate as a counter-ion. The thermal conductivity was found to be (1.3 ± 0.3) × 10-1 W/m·K. The highest figure of merit obtained at room temperature was (3.9 ± 1.0) × 10-2 using lithium perchlorate as a counter-ion. In addition, three-dimensional (3D) PEDOT nanonetworks were electropolymerized inside 3D anodic aluminum oxide (3D AAO), obtaining lower values in their thermoelectric properties.

Economic impact of pharmaceutical services on polymedicated patients: A systematic review.

BACKGROUND: Polypharmacy is commonly related to poor drug adherence, decreased quality of life and inappropriate prescribing in eldery. Furthermore, this condition also leads to a higher utilization of health services resources, due to the increased risk of adverse drug events, length of stays in hospitals and readmissions rates after discharge. OBJECTIVE: This Systematic Review aimed to synthesize the current evidence that evaluates pharmaceutical services on polymedicated patients, from an economic perspective. METHODS: Systematic searches were conducted in MEDLINE, SCOPUS and Cochrane Library databases to identify studies that were published until January 2021. Experimental and observational studies were included in this review, using strict inclusion/exclusion criteria and were assessed for quality using the following tools: RoB and ROBINS-I. Two independent reviewers selected the articles and extracted the data. RESULTS: 3,662 articles were retrieved from the databases. After the screening, 18 studies were included: 9 experimental and 9 observational studies. The studies reported that the integration of the pharmacist as a member of the healthcare team provides an optimized use of pharmacotherapy to polymedicated patients and contributes to health promotion, providing reduction of spending on medication, reduction of expenses related to emergency care and hospitalizations and other medical expenses. The ECRs made cost-effectiveness or cost-benefit analysis, and most of the Non Randomized studies had statistically significant cost savings even considering the expenses of pharmaceutical assistance. Experimental studies reported a cost reduction varying between US$ 193 to US$ 4,966 per patient per year. Furthermore, observational studies estimated a cost reduction of varying from US$ 3 to US$ 2,505 per patient per year. The cost savings are related to decrease in emergency visits and hospitalizations, through pharmacist intervention (medication review and pharmacotherapy follow-up). CONCLUSIONS: Considering the set of studies included, pharmaceutical care services directed to polymedicated patients may cooperate to save financial resources. Most of the interventions showed positive economic trends and also contributed to improving clinical parameters and quality of life. However, due to the majority of the studies having exploratory or qualitative methodology, it is essential to carry out more robust studies, based on full economic evaluation.

Experiências mediúnicas e dissociativas em Fernando Pessoa e C. G. Jung à luz da psicologia analítica e da psicologia anomalística / Mediumistic and dissociative experiences in Fernando Pessoa and C. G. Jung in light of analytical psychology and anomalistic psychology / Expériences médiumniques et dissociatives chez Fernando Pessoa et C. G. Jung à la lumière de la psychologie analytique et de la psychologie anomalistique / Experiencias mediúmnicas y disociativas en Fernando Pessoa y C. G. Jung a la luz de la psicología analítica y de la psicología anómala

Resumo O poeta Fernando Pessoa e o psiquiatra C. G. Jung desenvolveram suas obras em consonância com suas biografias. Ambos descreveram episódios que podem ser interpretados como mediúnicos, buscando, também, compreendê-los psicologicamente. Na literatura psicológica atual, a mediunidade pode ser entendida por meio do conceito de dissociação da personalidade. Dessa maneira, este artigo buscou examinar as relações entre dissociação e mediunidade a partir de episódios biográficos de Pessoa e de Jung, à luz da psicologia analítica e da psicologia anomalística. Hipotetiza-se que eles vivenciaram experiências dissociativas criativas e não patológicas, embora algum nível de sofrimento estivesse envolvido. Em Pessoa, as experiências dissociativas são relacionadas com sua produção hetoronímica, enquanto em Jung e suas investigações, com o desenvolvimento de seu modelo psicológico, que oferece elementos para um estudo psicobiográfico e artístico do poeta.

Abstract Poet Fernando Pessoa and psychiatrist C. G. Jung developed their works consonant with their biographies. Both described episodes that can be interpreted as mediumistic, seeking to understand them psychologically. In current psychological literature, mediumship is associated to the concept of personality dissociation. In this regard, this article examines the relationship between dissociation and mediumship based on Pessoa's and Jung's biographical episodes, in light of analytical psychology and anomalistic psychology. It is hypothesized that they experienced creative, non-pathological dissociations, although some level of suffering was involved. Pessoa's experiences are related to his hetoronymic production, whereas Jung's are tied with the development of his psychological model, which offers elements for a psychobiographical and artistic study of the poet.

Resumé Le poète Fernando Pessoa et le psychiatre C. G. Jung ont développé leurs œuvres en accord avec leurs biographies. Tous deux ont décrit des épisodes qui peuvent être interprétés comme médiumniques, en cherchant à les comprendre psychologiquement. Dans la littérature psychologique actuelle, la médiumnité est associée au concept de dissociation de la personnalité. À cet égard, cet article examine la relation entre dissociation et médiumnité à partir des épisodes biographiques de Pessoa et de Jung, à la lumière de la psychologie analytique et de la psychologie anomalistique. L'hypothèse est qu'ils ont fait l'expérience de dissociations créatives et non pathologiques, bien qu'un certain niveau de souffrance y ait été impliqué. Chez Pessoa, ces expériences sont liées à sa production hétéronymique, tandis que chez Jung, elles sont liées au développement de son modèle psychologique, ce qui offre des éléments pour une étude psychobiographique et artistic du poète.

Resumen El poeta Fernando Pessoa y el psiquiatra C. G. Jung desarrollaron sus obras en consonancia con sus biografías. Ambos describieron episodios que pueden interpretarse como mediúmnicos, también buscando comprenderlos psicológicamente. En la literatura de la psicología actual, la mediumnidad puede entenderse bajo el concepto de disociación de la personalidad. Así, este artículo buscó examinar la relación entre disociación y mediumnidad a partir de los episodios biográficos de Pessoa y Jung, a la luz de la psicología analítica y la psicología anomalística. La hipótesis supone que estos autores tuvieron experiencias disociativas creativas y no patológicas, aunque estas involucraron cierto nivel de sufrimiento. En Pessoa, las experiencias se relacionan con su producción de la heteronimia; y en Jung, con el desarrollo de su modelo psicológico, que ofrece elementos para un estudio psicobiográfico y artístico del poeta.

Diálogos entre C. G. Jung e William James: as pesquisas psíquicas / Diálogos entre C. G. Jung e William James: las investigaciones psíquicas / Dialogues between C. G. Jung e William James: the psychical researches

RESUMO. C. G. Jung e William James compartilhavam uma série de interesses de pesquisa. Por ocasião da Conferência realizada na Universidade de Clark, no ano de 1909, os dois autores tiveram a oportunidade de se encontrar e conversar. Os debates abordaram temas que não estavam na pauta da conferência, especialmente pesquisas psíquicas, também chamadas modernamente de experiências anomalísticas ou relacionadas à 'psi'. Desde seu período como estudante de medicina, Jung se interessou pelos fenômenos anômalos da consciência, tendo pesquisado os principais autores associados ao espiritualismo dos séculos XVIII e XIX. William James foi pesquisador reconhecido dos chamados fenômenos psíquicos, tendo participado de sociedades como a Society for Psychical Research e a American Society for Psychical Research. Através de seus estudos, James e Jung buscavam contribuir para a psicologia dinâmica, também chamada de psicologia profunda. O objetivo deste artigo foi ampliar os diálogos estabelecidos na universidade de Clark, resgatando informações importantes acerca da teoria dos dois autores.

RESUMEN. C. G. Jung y William James compartían una serie de intereses de investigación. En la conferencia celebrada en la Universidad de Clark, en 1909, los dos autores tuvieron la oportunidad de encontrarse y conversar. Los debates se centraron en temas que no estaban en la agenda de la conferencia, especialmente en la investigación psíquica, también llamada en la actualidad experiencia anomalística o relacionada con la psi. Jung de su tiempo como estudiante de medicina se interesó por fenómenos anómalos de conciencia, después de habiendo investigado los autores principales asociados con el espiritualismo de los siglos XVIII y XIX. William James era conocido investigador de los llamados fenómenos psíquicos, y participó en las sociedades como la Society for Psychical Research y la American Society for Psychical Research. James y Jung a través de sus estudios trataron de contribuir a la psicología dinámica, también llamada psicología profunda. El propósito de este artículo es ampliar el diálogo establecido en la Universidad de Clark, rescatando la información importante acerca de la teoría de los dos autores.

ABSTRACT. C.G. Jung and William James shared several research interests. At the conference held at Clark University in 1909, the two authors could meet and talk. The debates were especially on topics regarding psychical research, contemporarily also called anomalistic or psi-related experiences, which were not considered on the conference schedule. Since his period as a medical student, Jung has been interested in anomalous phenomena of consciousness, having researched the prominent authors associated with the spiritualism of the 18th and 19th centuries. William James was a recognized researcher of the so-called psychic phenomena, participating in societies such as Society for Psychical Research and the American Society for Psychical Research. Through their studies, James and Jung aspired to contribute to dynamic psychology, also called depth psychology. This article aimed to broaden the dialogues established at Clark University, rescuing important information about the theory of the two authors.

Avaliação da resistência à deformação plástica dos fios redondos de níquel-titânio / Evaluation of plastic deformation resistance of nickeltitanium round wires

Resumo As ligas de níquel-titânio (NiTi) possuem elasticidade elevada sem sofrer deformações plásticas permanentes, sendo amplamente indicadas nas primeiras fases do tratamento ortodôntico. O Objetivo deste estudo é avaliar a resistência de fios ortodônticos de NiTi termoativados em relação à deformação plástica. Foram avaliados fios de 6 marcas comerciais (GAC®, Morelli®, American Orthodontics®, Infinity®e 3M®). Primeiramente a partir de modelos prototipados foi simulada a relação entre deformação plástica e deflexão pela distância interbráquetes em 4 níveis de força a 37°C por 30 dias. Um segundo experimento foi realizado através da análise de fotografias padronizadas com diagramas observando o antes e depois de aplicada uma deformação ao fio a diferentes temperaturas. Os resultados do primeiro teste mostraram que todas as marcas comerciais testadas retornaram a sua forma original após sofrer deflexão por 30 dias. O segundo experimento mostrou que os fios da marca GAC® e American Orthodontics® não apresentaram diferenças estatisticamente significativas comparando as medidas do fio antes da deformação, deformado e após aquecimento (fio aquecido). Já os fios das marcas 3M®, Orthometric® e Infinity®, Morelli apresentaram diferenças significantes entre os fios novos e os fios deformados, e entre os fios deformados e os fios aquecidos, porém, não houve diferenças significantes entre os fios novos e aquecidos. Concluiu-se que nenhum fio, em nenhuma magnitude de deflexão aplicada, sofreu deformação plástica (permanente). Assim, todos os fios das marcas comerciais testadas foram considerados satisfatórios para a prática clínica da Ortodontia no que diz respeito à resistência a deformação plástica. (AU)

Abstract Nickel-titanium (NiTi) alloys have high elasticity without suffering permanent plastic deformations, widely indicated in the early stages of orthodontic treatment. This study aims to evaluate the heat-activated NiTi orthodontic wires' resistance to plastic deformation. The wire from 6 commercial brands (GAC®, Morelli®, American Orthodontics®, Infinity®, and 3M®) was evaluated first from prototype models, simulating the relationship between plastic deformation and deflection by the inter bracket distance at four levels of force at 37° C for 30 days. A second experiment was carried out by analyzing standardized photographs with diagrams observing the before and after deformation was applied to the wire at different temperatures. The first test showed that all tested trademarks returned to their original shape after deflecting for 30 days. The second experiment showed that GAC® and American Orthodontics® wires did not present statistically significant differences comparing the wire measurements before deformed deformation and after heating (heated wire). On the other hand, 3M®, Orthometric® and Infinity®, Morelli wires showed significant differences between new and deformed wires and between twisted and heated wires; however, there were no significant differences between new and heated wires. It was concluded that no wire, at any magnitude of applied deflection, suffered plastic (permanent) deformation. Thus, all wires of the commercial brands tested were considered satisfactory for the clinical practice of Orthodontics in terms of resistance to plastic deformation.(AU)

Synthesis and in Silico Modelling of the Potential Dual Mechanistic Activity of Small Cationic Peptides Potentiating the Antibiotic Novobiocin against Susceptible and Multi-Drug Resistant Escherichia coli.

Cationic antimicrobial peptides have attracted interest, both as antimicrobial agents and for their ability to increase cell permeability to potentiate other antibiotics. However, toxicity to mammalian cells and complexity have hindered development for clinical use. We present the design and synthesis of very short cationic peptides (3-9 residues) with potential dual bacterial membrane permeation and efflux pump inhibition functionality. Peptides were designed based upon in silico similarity to known active peptides and efflux pump inhibitors. A number of these peptides potentiate the activity of the antibiotic novobiocin against susceptible Escherichia coli and restore antibiotic activity against a multi-drug resistant E. coli strain, despite having minimal or no intrinsic antimicrobial activity. Molecular modelling studies, via docking studies and short molecular dynamics simulations, indicate two potential mechanisms of potentiating activity; increasing antibiotic cell permeation via complexation with novobiocin to enable self-promoted uptake, and binding the E. coli RND efflux pump. These peptides demonstrate potential for restoring the activity of hydrophobic drugs.

A katábasis de C. G. Jung: dos mitos antigos às experiências visionárias modernas / C. G. Jung's katabasis: from ancient myths to modern visionary experiences / La catábasis de C. G. Jung: de los mitos antiguos a las experiencias visionarias modernas

Este artigo buscou revisitar as experiências de katábasis de C.G. Jung, ou, em outras palavras, as experiências de descida ao submundo, ou mundo dos mortos, seguidas pelo retorno ao mundo dos vivos, a anábasis. Em termos psicológicos, essas experiências significam o confronto com o inconsciente e a subsequente ampliação da consciência. Para revisitar as experiências de katábasis de C.G. Jung, resgatou-se, historicamente, a katábasis (1) na antiguidade clássica através da mitologia grega, (2) no período medieval e moderno, por meio das obras de Dante Alighieri, Emmanuel Swedenborg e William Blake, e (3) finalmente, na própria vida de Jung, com ênfase na constituição de O Livro Vermelho. As experiências de katábasis foram de vital importância para Jung e culminaram na gênese da psicologia analítica. ■

This article aimed to revisit C.G. Jung experiences of katabasis, or in other words the experiences of descending to the underworld, or world of the dead, followed by the return to the world of the living, the anabasis. In psychological terms, these experiences mean confronting the unconscious and the subsequent expansion of consciousness. To revisit C.G. Jung's experiences of katabasis, it was rescued historically (1) in classical antiquity through greek mythology, (2) in the medieval and modern period, through the works of Dante Alighieri, Emmanuel Swedenborg and William Blake, and (3) finally in Jung's own life with an emphasis on the constitution of The Red Book. The katabasis experiences were of vital importance to Jung and culminated in the genesis of analytical psychology. ■

Este artículo buscó volver a examinar las experiencias de catábasis de C.G. Jung, o, en otras palabras, las experiencias de descender al inframundo, el mundo de los muertos, seguido por el retorno al mundo de los vivos, la anábasis. En términos psicológicos, estas experiencias suponen la confrontación con el inconsciente y la posterior expansión de la conciencia. Para volver a examinar las experiencias de catábasis de C.G. Jung, fue rescatada, históricamente, la catábasis (1) en la antigüedad clásica a través de la mitología griega, (2) en el período medieval y moderno, a través de las obras de Dante Alighieri, Emmanuel Swedenborg y William Blake, y (3) finalmente, en la propia vida de Jung, con énfasis en la constitución del Libro Rojo. Las experiencias de catábasis fueron de vital importancia para Jung y culminaron en la génesis de la psicología analítica. ■

Mps1-mediated release of Mad1 from nuclear pores ensures the fidelity of chromosome segregation.

The spindle assembly checkpoint (SAC) relies on the recruitment of Mad1-C-Mad2 to unattached kinetochores but also on its binding to Megator/Tpr at nuclear pore complexes (NPCs) during interphase. However, the molecular underpinnings controlling the spatiotemporal redistribution of Mad1-C-Mad2 as cells progress into mitosis remain elusive. Here, we show that activation of Mps1 during prophase triggers Mad1 release from NPCs and that this is required for kinetochore localization of Mad1-C-Mad2 and robust SAC signaling. We find that Mps1 phosphorylates Megator/Tpr to reduce its interaction with Mad1 in vitro and in Drosophila cells. Importantly, preventing Mad1 from binding to Megator/Tpr restores Mad1 accumulation at kinetochores, the fidelity of chromosome segregation, and genome stability in larval neuroblasts of mps1-null mutants. Our findings demonstrate that the subcellular localization of Mad1 is tightly coordinated with cell cycle progression by kinetochore-extrinsic activity of Mps1. This ensures that both NPCs in interphase and kinetochores in mitosis can generate anaphase inhibitors to efficiently preserve genomic stability.

The impact of James's Varieties of Religious Experience on Jung's work.

William James (1842-1910) is recognized as one of the main proponents of the then-emergent field of scientific psychology in the 19th century, and Carl Gustav Jung (1875-1961) is regarded as one of the most prolific authors within the fields of psychiatry and psychology of the 20th century. Previous studies have highlighted the practical and theoretical impact of James on the work of Jung. The present article makes use of detailed research for a deeper look at their theoretical relationship, with a focus on James's (1902/2010) Varieties of Religious Experience. This text, written in 1902, was particularly important for Jung's emphasis on fundamental subjective experience, which he evaluated in the book Psychology and Religion (Jung, 1938/1973). Moreover, we investigate important aspects of dynamic psychology developed by James, which Jung advanced in some of his works, particularly in "On the Nature of the Psyche" (Jung, 1946/1975a), an essay included in Structure & Dynamics of the Psyche. We focus on the idea that Jung's acquaintance with James led him to move away from psychoanalysis. In addition, their meeting shaped Jung's view regarding religious experience and influenced the formulation of his concept of the unconscious. (PsycINFO Database Record (c) 2020 APA, all rights reserved).

Comparative analysis of torque and apical force to assess the cutting behaviour of ProTaper Next and ProTaper Universal endodontic instruments.

This study assessed the cutting properties of two Nickel Titanium file systems with different designs and manufacturing materials. ProTaper Next X1 and X2 (PTN; Dentsply Sirona) and ProTaper Universal S2 and F1 (PTU; Dentsply Sirona) instruments were employed. The cutting parameters, that is torque and apical forces, were determined using a specially designed bench-testing machine. Dimensional and geometric parameters were considered to evaluate the results. The average maximum torque values showed statistically significant differences (P < 0.05) among all instruments. Apical force for PTN X2 and PTU F1 instruments initially decreased and then experienced a rapid increase in the last 2 mm of the canal. For PTN X1 and PTU S2, apical force increased during the entire test. When compared to PTU files, PTN required higher torque and apical forces during the shaping procedure. The results lead us to conclude that PTN instruments demonstrated a lower cutting efficiency than PTU.

Structural Characteristics and Torsional Resistance Evaluation of WaveOne and WaveOne Gold Instruments after Simulated Clinical Use.

INTRODUCTION: The aim of this study was to compare 2 reciprocating nickel-titanium systems with different manufacturing characteristics and to evaluate the influence of simulated clinical use on their torsional resistance. METHODS: New 25/.08 WaveOne Primary (WO; Dentsply Sirona, York, PA) and 25/.07 WaveOne Gold Primary (Dentsply Sirona) files were used in this study. The diameter and cross-sectional area at 3 mm from the tip were measured using image analyses. The alloy microstructure was evaluated by X-ray diffraction and the phase transformation temperatures by differential scanning calorimetry analyses. Forty files were divided into 4 groups: control group 1 and control group 2, which were submitted to torsion tests until rupture, and experimental group 1 and experimental group 2, in which instruments were used to shape 2 mesial canals of an extracted mandibular molar and then submitted to torsion tests until rupture. RESULTS: WO had higher values of diameter and area at 3 mm from the tip. X-ray diffraction analysis presented R-phase and austenite for both systems. Austenite finish temperatures, determined by differential scanning calorimetry, were above room temperature. WO showed higher values of torque until rupture, and WaveOne Gold showed higher values of angular deflexion (P > .05). No differences were found between the same type of instrument in the control and experimental groups. CONCLUSIONS: The higher torsional resistance of WO can be attributed to geometric characteristics. The use in 1 tooth with curved canals is safe and does not affect the torsional resistance of the instruments analyzed.

William James e Carl Gustav Jung na conferência em Clark: repercussões teóricas / William James and Carl Gustav Jung in the conference at Clark: theoretical repercussions / William James y Carl Gustav Jung en la conferencia de Clark: repercusiones teóricas

A obra de Carl Gustav Jung ainda é pouco pesquisada no contexto acadêmico. Devido à escassez de estudos sobre a fundamentação teórica do referido autor, muitos consideram seu trabalho como influenciado somente pela psicanálise freudiana. No entanto o próprio Jung, ao longo de suas obras coligidas, destaca a figura de William James como o autor que mais o incentivou em suas pesquisas. Em 1909, ainda inserido no movimento psicanalítico, o autor suíço viaja para a América, onde apresenta, na Conferência da Universidade de Clark, seu conhecido estudo sobre associações de palavras. Na referida conferência, Jung conhece William James. A partir desse encontro histórico, as referências ao pensador americano se intensificam. O objetivo desse trabalho é destacar os principais temas da teoria jameseana que repercutem significativamente sobre a psicologia de C. G. Jung, especialmente, seus estudos psiquiátricos, sua avaliação do pragmatismo, sua teoria do inconsciente, teoria das emoções e experiência religiosa.

The work of Carl Gustav Jung is still poorly researched in the academic context. Due to the lack of studies on the author’s theoretical foundation, many consider his work as influenced only by Freudian psychoanalysis. However, Jung himself, along his collected works, points the figure of William James as the author who most encouraged him in his researches. In 1909, still inserted in the psychoanalytic movement, the Swiss author travels to America, where he presents, in the Clark University Conference, his well-known study of word associations. In this conference, Jung meets William James. From that historic meeting the references to the American thinker intensify. The aim of this study is to highlight the main themes of Jamesian theory that impact significantly on the C. G. Jung’s psychology, especially his psychiatric studies, review of pragmatism, his theory of the unconscious, theory of emotions and religious experience.

La obra de Carl Gustav Jung es poco investigada en el contexto académico. Debido a la falta de estudios sobre el fundamento teórico de este autor, muchos consideran su trabajo como influenciado solamente por el psicoanálisis de Freud. Sin embargo, el propio Jung, en sus obras completas, enfatiza la figura de William James como el autor que más le animó en sus investigaciones. En 1909, aún insertado en el movimiento psicoanalítico, el autor suizo viaja a Estados Unidos donde presenta, en la Conferencia de la Universidad de Clark, su conocido estudio de asociaciones de palabras. En esta conferencia, Jung conoce a William James. A partir de este encuentro histórico, las referencias al pensador americano se intensifican. El objetivo de este trabajo es poner de relieve los principales temas de la teoría jameseana que inciden significativamente en la psicología de C. G. Jung, en especial, sus estudios psiquiátricos, su revisión del pragmatismo, su teoría del inconsciente, la teoría de las emociones y la experiencia religiosa.

Cyclic flexural fatigue resistance of NiTi Controlled Memory and Blue Technology instruments after torsional preloading.

Objective The aim of this study was to evaluate the influence of torsional preloading on the cyclic flexural fatigue resistance of thermally treated NiTi instruments. Material and Methods Ten new instruments New Hyflex CM (HF 30.06; Coltene/Whaladent Inc.), Typhoon CM (TYP 30.06; Clinician's Choice Dental Products) and Vortex Blue (VB 30.06; Dentsply Tulsa Dental) were chosen, based on geometry and specific characteristics of the manufacturing process. The new instruments of each system were tested in a bench device to determine their fatigue resistance through mean value of number of cycles to failure (Nf) (Control Group - CG). Another group of 10 new HF, TYP and VB instruments were submitted to 20 cycles of torsional straining between 0° and 180° (Experimental Group - EG) and then submitted to fatigue until rupture under the same conditions of the CG. Tested instruments were examined by scanning electron microscopy (SEM). Data were analyzed using one-way analysis of variance and post hoc Tukey's test (α=.05). Results Higher fatigue resistance was accomplished by HF instruments, followed by VB and TYP (p<0.05). During the torsional preloading, the lowest mean torque value was observed for TYP instruments (p<0.05). The torsional preload caused a significant reduction in the Nf values (p<0.05) of about 20%, 39% and 45% for instruments HF, VB and TYP, respectively. Longitudinal cracks, generated during the torsional preloading, were present in VB files, but were not observed in the CM instruments (HF and TYP). Conclusions In conclusion, the flexural fatigue resistance of thermally treated instruments is diminished after cyclic torsional loading.