Application of bacteriophages in biopolymer-based functional food packaging films.

Recently, food spoilage caused by pathogens has been increasing. Therefore, applying control strategies is essential. Bacteriophages can potentially reduce this problem due to their host specificity, ability to inhibit bacterial growth, and extend the shelf life of food. When bacteriophages are applied directly to food, their antibacterial activity is lost. In this regard, bacteriophage-loaded biopolymers offer an excellent option to improve food safety by extending their shelf life. Applying bacteriophages in food preservation requires comprehensive and structured information on their isolation, culturing, storage, and encapsulation in biopolymers for active food packaging applications. This review focuses on using bacteriophages in food packaging and preservation. It discusses the methods for phage application on food, their use for polymer formulation and functionalization, and their effect in enhancing food matrix properties to obtain maximum antibacterial activity in food model systems.

Recent Progress of Carrageenan-Based Composite Films in Active and Intelligent Food Packaging Applications.

Recently, as concerns about petrochemical-derived polymers increase, interest in biopolymer-based materials is increasing. Undoubtedly, biopolymers are a better alternative to solve the problem of synthetic polymer-based plastics for packaging purposes. There are various types of biopolymers in nature, and mostly polysaccharides are used in this regard. Carrageenan is a hydrophilic polysaccharide extracted from red algae and has recently attracted great interest in the development of food packaging films. Carrageenan is known for its excellent film-forming properties, high compatibility and good carrier properties. Carrageenan is readily available and low cost, making it a good candidate as a polymer matrix base material for active and intelligent food packaging films. The carrageenan-based packaging film lacks mechanical, barrier, and functional properties. Thus, the physical and functional properties of carrageenan-based films can be enhanced by blending this biopolymer with functional compounds and nanofillers. Various types of bioactive ingredients, such as nanoparticles, natural extracts, colorants, and essential oils, have been incorporated into the carrageenan-based film. Carrageenan-based functional packaging film was found to be useful for extending the shelf life of packaged foods and tracking spoilage. Recently, there has been plenty of research work published on the potential of carrageenan-based packaging film. Therefore, this review discusses recent advances in carrageenan-based films for applications in food packaging. The preparation and properties of carrageenan-based packaging films were discussed, as well as their application in real-time food packaging. The latest discussion on the potential of carrageenan as an alternative to traditionally used synthetic plastics may be helpful for further research in this field.

Active Fish Gelatin/Chitosan Blend Film Incorporated with Guava Leaf Powder Carbon Dots: Properties, Release and Antioxidant Activity.

Active packaging is an innovative approach to prolonge the shelf-life of food products while ensuring their quality and safety. Carbon dots (CDs) from biomass as active fillers for biopolymer films have been introduced to improve their bioactivities as well as properties. Gelatin/chitosan (G/C) blend films containing active guava leaf powder carbon dots (GL-CDs) at various levels (0-3%, w/w) were prepared by the solvent casting method and characterized. Thickness of the control increased from 0.033 to 0.041 mm when 3% GL-CDs were added (G/C-CD-3%). Young's modulus of the resulting films increased (485.67-759.00 MPa), whereas the tensile strength (26.92-17.77 MPa) and elongation at break decreased (14.89-5.48%) as the GL-CDs' level upsurged (p < 0.05). Water vapor barrier property and water contact angle of the film were enhanced when incorporated with GL-CDs (p < 0.05). GL-CDs had a negligible impact on film microstructure, while GL-CDs interacted with gelatin or chitosan, as determined by FTIR. The release of GL-CDs from blend films was more pronounced in water than in alcoholic solutions (10-95% ethanol). The addition of GL-CDs improved the UV light barrier properties and antioxidant activities of the resultant films in a dose-dependent manner. Thus, GL-CD-added gelatin/chitosan blend films with antioxidant activities could be employed as potential active packaging for the food industry.

Storage stability of Asian seabass oil-in-water Pickering emulsion packed in pouches made from electrospun and solvent casted bilayer films from poly lactic acid/chitosan-gelatin blend containing epigallocatechin gallate.

Bilayer pouches were fabricated with chitosan (CS)-fish gelatin (FG) mixture containing epigallocatechin gallate (EGCG) deposited over the poly lactic acid (PLA) film through solvent casting and electrospinning techniques. Pickering emulsions (PE) of Asian seabass depot fat oil stabilized by zein colloidal particles were packed in bilayer pouches and stored at 28 ± 2 °C. The PE packed in pouch containing EGCG had higher emulsion and oxidative stability after 30 days of storage as witnessed by the smaller droplet size and lower values of thiobarbituric acid reactive substances, peroxide, conjugated diene and volatile compounds in comparison with control (PE packed in monolayer PLA pouch) (P < 0.05). EGCG incorporated pouch retained more linoleic acid (C18:2 n-6) and linolenic acid (C18:3 n-9) in emulsion than PLA pouch. Therefore, pouch from bilayer PLA/CS-FG films comprising EGCG could serve as active packaging and extended the shelf life of Pickering emulsion.

Sulfur quantum dots as sustainable materials for biomedical applications: Current trends and future perspectives.

Discovered over a decade ago, sulfur quantum dots (SQDs) have rapidly emerged as a sustainable, safe, and inexpensive quantum material. Sustainably synthesizing SQDs using sublimed sulfur powders, typically produced as waste in industrial petrochemical refining processes, has attracted researchers to use these functional quantum materials in various research fields. SQDs quickly found applications in various research fields, such as electronics, environmental sensing, food packaging, and biomedical engineering. Although low production yields, time-consuming and energy-intensive synthetic methods, and low photoluminescence quantum yield (PLQY) have been some problems, researchers have found ways to improve synthetic methods, develop passivating agents, and systematically modify reaction schemes and energy sources to achieve large-scale synthesis of stable SQDs with high PLQY. Nonetheless, SQDs have succeeded tremendously in biomedical and related applications due to their low toxicity, antibacterial and antioxidant properties, biocompatibility, appropriate cellular uptake, and photoluminescent properties. Although the bioimaging applications of SQDs have been extensively studied, their other reported properties indicate their suitability for use as antimicrobial agents, free radical scavengers, and drug carriers in other biomedical applications, such as tissue regeneration, wound healing, and targeted drug delivery.

In-package cold plasma treatment to extend the shelf life of food.

Conventional food preservation methods such as heat treatment, irradiation, chemical treatment, refrigeration, and coating have various disadvantages, like loss of food quality, nutrition, and cost-effectiveness. Accordingly, cold plasma is one of the new technologies for food processing and has played an important role in preventing food spoilage. Specifically, in-package cold plasma has become a modern trend to decontaminate, process, and package food simultaneously. This strategy has proven successful in processing various fresh food ingredients, including spinach, fruits, vegetables, and meat. In particular, cold plasma treatment within the package reduces the risk of post-processing contamination. Cryoplasm decontamination within packaging has been reported to reduce significantly the microbial load of many foods' spoilage-causing pathogens. However, studies are needed to focus more on the effects of in-package treatments on endogenous enzyme activity, pest control, and removal of toxic pesticide residues. In this review, we comprehensively evaluated the efficacy of in-package low-temperature plasma treatment to extend the shelf life of various foods. The mechanisms by which cold plasma interacts with food were investigated, emphasizing its effects on pathogen reduction, spoilage mitigation, and surface modification. The review also critically assessed the effects of the treatments on food quality, regulatory considerations, and their potential as viable technologies to improve food safety and packaging life. In-package cold plasma treatment could revolutionize food storage when combined with other sophisticated technologies such as nanotechnology.

Advances in sustainable food packaging applications of chitosan/polyvinyl alcohol blend films.

Researchers are addressing environmental concerns related to petroleum-based plastic packaging by exploring biopolymers from natural sources, chemical synthesis, and microbial fermentation. Despite the potential of individual biopolymers, they often exhibit limitations like low water resistance and poor mechanical properties. Blending polymers emerges as a promising strategy to overcome these challenges, creating films with enhanced performance. This review focuses on recent advancements in chitosan/polyvinyl alcohol (PVA) blend food packaging films. It covers molecular structure, properties, strategies for performance improvement, and applications in food preservation. The blend's excellent compatibility and intermolecular interactions make it a promising candidate for biodegradable films. Future research should explore large-scale thermoplastic technologies and investigate the incorporation of additives like natural extracts and nanoparticles to enhance film properties. Chitosan/PVA blend films offer a sustainable alternative to petroleum-based plastic packaging, with potential applications in practical food preservation.

Carrageenan-based active and intelligent packaging films integrated with anthocyanin and TiO2-doped carbon dots derived from sweet potato peels.

Carrageenan-based sustainable active and pH-dependent color-changing composite films were fabricated by blending anthocyanin extracted from sweet potato peel (SPA) with TiO2-doped carbon dots (Ti-CDs) prepared using the biowaste of SPA extraction. The SPA and Ti-CDs were compatible with the carrageenan matrix and were uniformly dispersed in the used polymer to form a homogeneous film with increased mechanical properties. The composite film added with SPA and 3 wt% Ti-CD showed 100 % UV protection, superb antioxidant (100 % DPPH and ABTS scavenging assay), and potent antibacterial activity (complete eradication of foodborne L.monocytogenes and E. coli strains after 3 h incubation). Additionally, the composite films showed distinguishable colorimetric responses to pH 7-12 buffers and volatile ammonia. The intelligent sensing ability of the composite film was assessed through shrimp freshness monitoring, and the film's hue shifted from pink (fresh shrimp) to yellow/brown (inedible shrimp) during storage. Shrimp packaging studies have shown that composite films retard the rate of food quality change during storage and are a good indicator of shrimp spoilage. Therefore, the designed film is expected to have high applicability as a chip, and quick on-site sensor that detects seafood quality in real-time, and a highly effective multifunctional film for better product quality preservation.

Sustainable packaging film based on cellulose nanofibres/pullulan impregnated with zinc-doped carbon dots derived from avocado peel to extend the shelf life of chicken and tofu.

A cellulose nanofiber (CNF)/pullulan (PUL) based multifunctional composite film was developed for active packaging applications by incorporating Zn-doped avocado-derived carbon dots (Zn-ACDs). The incorporation of Zn-ACDs improved the interfacial compatibility and produced a dense cross-sectional structure of the composite films. The Zn-ACDs added film showed no significant difference in water vapor permeability and surface hydrophilicity compared to the neat CNF/PUL film, but the tensile strength and elongation at break increased by ~45.4 % and ~64.1 %, respectively. The addition of 5 wt% Zn-ACDs to the CNF/PUL matrix resulted in 100.0 % UV blocking properties, excellent antioxidant activity (100.0 % for ABTS and 68.0 % for DPPH), and complete eradication of foodborne pathogens such as Listeria monocytogenes ATCC 15313 and Escherichia coli O157:H7 after 3 h of exposure. The CNF/PUL composite film with Zn-ACDs applied to the active packaging of chicken and tofu significantly reduced the total growth of aerobic microorganisms without significantly changing the actual color of the packaged chicken and tofu for 9 days at 10°C. This study demonstrates that CNF/PUL composite films with Zn-ACDs are a sustainable and environmentally friendly option for protecting food from microbial contamination.

Anti-inflammatory and antioxidant activity of elemental sulfur-derived sustainable sulfur quantum dots.

Sulfur quantum dots (SQDs) are attracting increasing attention in the biomedical field due to their unique properties, such as antibacterial activity, free radical scavenging potential, optical properties, biocompatibility, and non-toxicity. Ethylenediamine passivated SQDs (ED-SQDs) were synthesized using a hydrothermal method. Cytotoxicity evaluation of ED-SQDs on RAW264.7 cells showed more than 90% cell viability even at 500 µg/mL of ED-SQDs, with an established IC50 value of 880.9 µg/mL. In addition, ED-SQDs showed potent antioxidant activity in vitro, effectively scavenging ABTS and DPPH free radicals at concentrations below 100 µg/mL, comparable to ascorbic acid. ED-SQD reduced lipopolysaccharide (LPS)-induced nitric oxide and reactive oxygen species in macrophages, lowered pro-inflammatory cytokines, and inactivated LPS-activated STAT3. In addition, ED-SQD increased nuclear NRF2 and the expression of genes encoding antioxidant enzymes in LPS-stimulated cells. These results reveal the antioxidant and anti-inflammatory potential of ED-SQDs at non-toxic concentrations, providing evidence for their potential anti-inflammatory applications.

Carboxymethylcellulose/Agar-Based Multifunctional Films Incorporated with Zn-Doped SnO2 Nanoparticles for Active Food Packaging Application.

SnO2 and Zn-SnO2 nanoparticles were prepared by chemical precipitation, and the rutile phase of SnO2 was confirmed through X-ray diffraction studies. X-ray photoelectron spectroscopy (XPS) confirmed the doping of SnO2 with Zn and elucidated the surface chemistry before and after doping. The average sizes of SnO2 and Zn-SnO2 nanoparticles determined using TEM were 3.96 ± 0.85 and 3.72 ± 0.9 nm, respectively. UV-visible and photoluminescence spectrophotometry were used to evaluate the optical properties of SnO2 and Zn-SnO2 nanoparticles, and their energy gaps (Eg) were 3.8 and 3.9 eV, respectively. The antibacterial activity of these nanoparticles against Salmonella enterica and Staphylococcus aureus was evaluated under dark and light conditions. Antibacterial activity was higher in light, showing the highest activity (99.5%) against S. enterica. Carboxymethylcellulose (CMC)/agar-based functional composite films were prepared by adding different amounts of SnO2 and Zn-SnO2 nanoparticles (1 and 3 wt % of polymers). The composite film showed significantly increased UV barrier properties while maintaining the mechanical properties, water vapor barrier, and transparency compared to the neat CMC/agar film. These composite films showed significant antibacterial activity; however, the Zn-SnO2-added film showed stronger antibacterial activity (99.2%) than the SnO2-added film (15%).

Effects of packaging and temperature abuse on the quality of red pepper (Capsicum annuum L.) powder.

Storage stability of pepper (Capsicum annuum L.) powder packaged using 2 different film pouches of Ny/PE and PET/Al/PE inserted with moisture absorbent and oxygen scavenger was investigated during storage at 25 °C for 5 months and at 40 °C for 14 days. The moisture content of red pepper powder did not change significantly in PET/Al/PE packaging but decreased significantly in Ny/PE packaging after the abuse of storage temperature. The color of red pepper powder was quite stable in all packaging treatments. Other quality characteristics of all packaged pepper powder, including microbial cell count, capsaicinoids, ascorbic acid, and free sugar content, were also maintained near their initial levels with no appreciable changes during storage. Red pepper powder with a moisture content of 13-14% and packaged with a film with high gas-barrier properties can be stored for more than 5 months even if there is an unexpected temperature abuse during storage.

Gelatin/poly(vinyl alcohol)-based dual functional composite films integrated with metal-organic frameworks and anthocyanin for active and intelligent food packaging.

Innovative active and pH-colorimetric composite films were fabricated from gelatin/poly(vinyl alcohol) (Gel/PVA) integrated with copper-based metal-organic frameworks (Cu-MOFs) and red cabbage anthocyanin (RCA). The incorporation of Cu-MOFs improved the tensile strength, water resistance, and UV shielding properties of the developed composite films. The addition of anthocyanins and 3 wt% Cu-MOFs endowed the polymer matrix with excellent antioxidant (100 % against ABTS and DPPH radicals) and antibacterial (against Gram-positive and Gram-negative foodborne pathogenic bacteria) functions. The fabricated composite films exhibited significant color change at alkaline conditions of pH 7-12 and a marked color change upon exposure to ammonia. The designed indicator films used for shrimp freshness tracking and a visual color change from pink (for fresh shrimp) to green (for spoiled shrimp) was observed during storage at 28 °C for 24 h. The potential applications of the engineered composite films were studied by shrimp packaging, and the quality parameters of packaged samples were monitored during storage. The synergistic effects of adding anthocyanins and MOF nanostructures works for better product freshness preservation and responds well to shrimp spoilage level, introducing novel active and intelligent packaging options for practical smart packaging applications.

Encapsulation and delivery systems of cinnamon essential oil for food preservation applications.

Food safety threats and deterioration due to the invasion of microorganisms has led to economic losses and food-borne diseases in the food industry; so, development of natural food preservatives is urgently needed when considering the safety of chemically synthesized preservatives. Because of its outstanding antioxidant and antibacterial properties, cinnamon essential oil (CEO) is considered a promising natural preservative. However, CEO's low solubility and easy degradability limits its application in food products. Therefore, some encapsulation and delivery systems have been developed to improve CEO efficiency in food preservation applications. This work discusses the chemical and techno-functional properties of CEO, including its key components and antioxidant/antibacterial properties, and summarizes recent developments on encapsulation and delivery systems for CEO in food preservation applications. Since CEO is currently added to most biopolymeric films/coatings (BFCs) for food preservation, most studies have shown that encapsulation systems can improve the food preservation performance of BFCs containing CEOs. It has been confirmed that various delivery systems could improve the stability and controlled-release properties of CEO, thereby enhancing its ability to extend the shelf life of foods. These encapsulation techniques include spray drying, emulsion systems, complex coacervation (nanoprecipitation), ionic gelation, liposomes, inclusion complexation (cyclodextrins, silica), and electrospinning.

Facile Synthesis of Multifunctional Carbon Dots from Spent Gromwell Roots and Their Application as Coating Agents.

Spent Gromwell root-based multifunctional carbon dots (g-CDs) and sulfur-functionalized g-CDs (g-SCDs) were synthesized using a hydrothermal method. The mean particle size of g-CDs was confirmed to be 9.1 nm by TEM (transmission electron microscopy) analysis. The zeta potentials of g-CDs and g-SCDs were mostly negative with a value of -12.5 mV, indicating their stability in colloidal dispersion. Antioxidant activities were 76.9 ± 1.6% and 58.9 ± 0.8% for g-CDs, and 99.0 ± 0.1% and 62.5 ± 0.5% for g-SCDs by 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging tests, respectively. In addition, the bathochromic shift of g-CDs is observed when their emission peaks appear at a higher wavelength than the excitation peaks. The prepared g-CDs and g-SCDs solutions were used as a coating agent for potato slices. The browning index of the control potato slices increased significantly from 5.0 to 33.5% during 24 to 72 h storage. However, the sample potato slices coated with g-CDs or g-SCDs suppressed the increase in the browning index. In particular, the browning index of the potato slices coated with g-SCDs ranged from 1.4 to 5.5%, whereas the potato slices coated with g-CDs had a browning index ranging from 3.5 to 26.1%. The g-SCDs were more effective in delaying oxidation or browning in foods. The g-CDs and g-SCDs also played a catalytic role in the Rhodamine B dye degradation activity. This activity will be useful in the future to break down toxins and adulterants in food commodities.

Preparation of chitosan/gelatin-based functional films integrated with carbon dots from banana peel for active packaging application.

Carbon dots (CDs) were manufactured with banana peels using a hydrothermal method (200 °C for 6 h). The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface. CDs have been impregnated into chitosan/gelatin films to synthesize multifunctional packaging films. The composite film showed a slight decrease in transparency but a significant increase in UV protection properties. The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential. The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure. The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C. The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.

Preparation of milk-based probiotic lactic acid bacteria biofilms: A new generation of probiotics.

Biofilm is considered as a community of microorganisms in which cells adhere to each other on surfaces in a self-produced matrix of extracellular polymer compounds. In recent years, efforts to use the beneficial aspects of biofilm in probiotic research have intensified. In this study, probiotic biofilms of Lactiplantibacillus plantarum and Lacticaseibacillus rhamnosus were manufactured using milk and transferred to yogurt in whole and pulverized forms to test in real food conditions. Survival was assessed during 21 days of storage time as well as gastrointestinal conditions. The results indicated that Lp. plantarum and Lc. rhamnosus can form a very desirable and strong biofilm that can have a good protective effect on the survival of these bacteria in probiotic yogurt during processing, storage, and gastrointestinal conditions, in a way that, after 120 min of treatment in high acidic gastrointestinal conditions (pH 2.0), the survival rate decreased by only 0.5 and 1.1 log CFU/ml. Probiotic biofilm can be used as a natural way of utilizing bacteria in biotechnology and fermentation, which is an excellent way to increase the utility of probiotics.

Recent progress in pectin extraction, characterization, and pectin-based films for active food packaging applications: A review.

Pectin is an abundant complex polysaccharide obtained from various plants. Safe, biodegradable, and edible pectin has been extensively utilized in the food industry as a gelling agent, thickener, and colloid stabilizer. Pectin can be extracted in a variety of ways, thus affecting its structure and properties. Pectin's excellent physicochemical properties make it suitable for many applications, including food packaging. Recently, pectin has been spotlighted as a promising biomaterial for manufacturing bio-based sustainable packaging films and coatings. Functional pectin-based composite films and coatings are useful for active food packaging applications. This review discusses pectin and its use in active food packaging applications. First, basic information and characteristics of pectin, such as the source, extraction method, and structural characteristics, were described. Then, various methods of pectin modification were discussed, and the following section briefly described pectin's physicochemical properties and applications in the food sector. Finally, the recent development of pectin-based food packaging films and coatings and their use in food packaging were comprehensively discussed.

New opportunities and advances in quercetin-added functional packaging films for sustainable packaging applications: a mini-review.

Recently, research on functional packaging films and their application to food preservation has been actively conducted. This review discusses recent advances and opportunities for using quercetin in developing bio-based packaging films for active food packaging. Quercetin is a plant-based yellow pigment flavonoid with many useful biological properties. Quercetin is also a GRAS food additive approved by the US FDA. Adding quercetin to the packaging system improves the physical performance as well as the functional properties of the film. Therefore, this review focused on quercetin's effect on the various packaging film properties, such as mechanical, barrier, thermal, optical, antioxidant, antimicrobial, and so on. The properties of films containing quercetin depend on the type of polymer and the interaction between the polymer and quercetin. Films functionalized with quercetin are useful in extending shelf life and maintaining the quality of fresh foods. Quercetin-added packaging systems can be very promising for sustainable active packaging applications.

Chitosan/Starch-Based Active Packaging Film with N, P-Doped Carbon Dots for Meat Packaging.

Nitrogen, phosphorus-doped green-tea-derived carbon dots (NP-CDs) incorporated chitosan/starch (Chi/St) based multifunctional nanocomposite films were prepared. FE-SEM images verified a homogeneous distribution of CDs with minimum aggregation in the fabricated films. Incorporating NP-CDs led to enhanced UV-light blocking (93.1% of UV-A and ∼99.7% of UV-B) without significantly affecting the films' water transparency and water vapor permeability. Besides, incorporating NP-CDs into the Chi/St films enhanced antioxidant activity (98.0% for ABTS and 71.4% for DPPH) and displayed strong antibacterial activity against L. monocytogenes, E. coli, and S. aureus. Wrapping the meat in the prepared film and storing it at 20 °C has been shown to reduce bacterial growth (less than 2.5 Log CFU/g after 48 h) without significantly altering the actual color of the wrapped meat. The Chi/St film loaded with NP-CD has high potential as an active packaging material to ensure safety and extend the shelf life of meat products.