Estimation of leaf area index using PROSAIL based LUT inversion, MLRA-GPR and empirical models: Case study of tropical deciduous forest plantation, North India.

Forests play a vital role in biological cycles and environmental regulation. To understand the key processes of forest canopies (e.g., photosynthesis, respiration and transpiration), reliable and accurate information on spatial variability of Leaf Area Index (LAI), and its seasonal dynamics is essential. In the present study, we assessed the performance of biophysical parameter (LAI) retrieval methods viz. Look-Up Table (LUT)-inversion, MLRA-GPR (Machine Learning Regression Algorithm-Gaussian Processes Regression) and empirical models, for estimating the LAI of tropical deciduous plantation using ARTMO (Automated Radiative Transfer Models Operator) tool and Sentinel-2 satellite images. The study was conducted in Central Tarai Forest Division, Haldwani, located in the Uttarakhand state, India. A total of 49 ESUs (Elementary Sampling Unit) of 30m×30m size were established based on variability in composition and age of plantation stands. In-situ LAI was recorded using plant canopy imager during the leaf growing, peak and senescence seasons. The PROSAIL model was calibrated with site-specific biophysical and biochemical parameters before used to the predicted LAI. The plantation LAI was also predicted by an empirical approach using optimally chosen Sentinel-2 vegetation indices. In addition, Sentinel-2 and MODIS LAI products were evaluated with respect to LAI measurements. MLRA-GPR offered best results for predicting LAI of leaf growing (R2 = 0.9, RMSE = 0.14), peak (R2 = 0.87, RMSE = 0.21) and senescence (R2 = 0.86, RMSE = 0.31) seasons while LUT inverted model outperformed VI's based parametric regression model. Vegetation indices (VIs) derived from 740 nm, 783 nm and 2190 nm band combinations of Sentinel-2 offered the best prediction of LAI.

Retrieval of canopy water content of different crop types with two new hyperspectral indices: Water Absorption Area Index and Depth Water Index.

Crop canopy water content (CWC) is an essential indicator of the crop's physiological state. While a diverse range of vegetation indices have earlier been developed for the remote estimation of CWC, most of them are defined for specific crop types and areas, making them less universally applicable. We propose two new water content indices applicable to a wide variety of crop types, allowing to derive CWC maps at a large spatial scale. These indices were developed based on PROSAIL simulations and then optimized with an experimental dataset (SPARC03; Barrax, Spain). This dataset consists of water content and other biophysical variables for five common crop types (lucerne, corn, potato, sugar beet and onion) and corresponding top-of-canopy (TOC) reflectance spectra acquired by the hyperspectral HyMap airborne sensor. First, commonly used water content index formulations were analysed and validated for the variety of crops, overall resulting in a R2 lower than 0.6. In an attempt to move towards more generically applicable indices, the two new CWC indices exploit the principal water absorption features in the near-infrared by using multiple bands sensitive to water content. We propose the Water Absorption Area Index (WAAI) as the difference between the area under the null water content of TOC reflectance (reference line) simulated with PROSAIL and the area under measured TOC reflectance between 911 and 1271 nm. We also propose the Depth Water Index (DWI), a simplified four-band index based on the spectral depths produced by the water absorption at 970 and 1200 nm and two reference bands. Both the WAAI and DWI outperform established indices in predicting CWC when applied to heterogeneous croplands, with a R2 of 0.8 and 0.7, respectively, using an exponential fit. However, these indices did not perform well for species with a low fractional vegetation cover (< 30%). HyMap CWC maps calculated with both indices are shown for the Barrax region. The results confirmed the potential of using generically applicable indices for calculating CWC over a great variety of crops.

Stability of a Parenteral Formulation of Betamethasone and Levobupivacaine.

Background: The therapeutic management of syndromes presenting simultaneously pain and inflammation often requires the administration of anesthetic and corticosteroid drugs by epidural administration. In this article, we studied a mixture that combines betamethasone and levobupivacaine, which demonstrates prolonged analgesic effects. To our knowledge, the stability of such a mixture in epidural solution has not been examined. Objective: To evaluate the chemical, physical, and microbiological stability of an extemporaneously prepared mixture. Methods: A solution of betamethasone acetate 1 mg/mL, betamethasone phosphate 1 mg/mL, and levobupivacaine hydrochloride 0.83 mg/mL was prepared in saline. The components were analyzed by high-performance liquid chromatography for up to 270 days of storage, protected and exposed to light, at room temperature, and stored in the refrigerator and at 45°C. In addition, sterility, organoleptic properties, and pH of the admixture were monitored. Results: There are no significant differences between drug concentrations obtained at room temperature and at refrigerated temperature. The accelerated conditions (45°C) demonstrated different results among the actives: betamethasone acetate and levobupivacaine hydrochloride are affected while betamethasone phosphate remains stable. The stability of the mixture does not depend on light exposure. The validity period of the different components in the mixture was estimated as 120 days for betamethasone phosphate and 163 days for levobupivacaine hydrochloride; betamethasone acetate remained unchanged during 155 days. Conclusion: Analgesic mixtures of betamethasone and levobupivacaine can be stored at ambient temperature in polypropylene vials for up to 120 days at the studied concentrations. These data enable the rationalization of the centralized preparation in the hospital pharmacy.

Gaussian processes retrieval of leaf parameters from a multi-species reflectance, absorbance and fluorescence dataset.

Biochemical and structural leaf properties such as chlorophyll content (Chl), nitrogen content (N), leaf water content (LWC), and specific leaf area (SLA) have the benefit to be estimated through nondestructive spectral measurements. Current practices, however, mainly focus on a limited amount of wavelength bands while more information could be extracted from other wavelengths in the full range (400-2500nm) spectrum. In this research, leaf characteristics were estimated from a field-based multi-species dataset, covering a wide range in leaf structures and Chl concentrations. The dataset contains leaves with extremely high Chl concentrations (>100µgcm(-2)), which are seldom estimated. Parameter retrieval was conducted with the machine learning regression algorithm Gaussian Processes (GP), which is able to perform adaptive, nonlinear data fitting for complex datasets. Moreover, insight in relevant bands is provided during the development of a regression model. Consequently, the physical meaning of the model can be explored. Best estimates of SLA, LWC and Chl yielded a best obtained normalized root mean square error of 6.0%, 7.7%, 9.1%, respectively. Several distinct wavebands were chosen across the whole spectrum. A band in the red edge (710nm) appeared to be most important for the estimation of Chl. Interestingly, spectral features related to biochemicals with a structural or carbon storage function (e.g. 1090, 1550, 1670, 1730nm) were found important not only for estimation of SLA, but also for LWC, Chl or N estimation. Similar, Chl estimation was also helped by some wavebands related to water content (950, 1430nm) due to correlation between the parameters. It is shown that leaf parameter retrieval by GP regression is successful, and able to cope with large structural differences between leaves.