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1.
Rev Esp Enferm Dig ; 2024 May 20.
Article in English | MEDLINE | ID: mdl-38767014

ABSTRACT

Intestinal malrotation is the most common congenital anomaly of the small intestine. However, it is associated with delayed diagnosis due to the lack of specificity of its symptoms, which can lead to devastating consequences such as intestinal volvulus or massive intestinal necrosis. We present a clinical case in which we highlight the importance of abdominal computed tomography and the detection of its characteristic signs for the early identification of this pathology.

2.
Int Nurs Rev ; 2024 Apr 25.
Article in English | MEDLINE | ID: mdl-38661539

ABSTRACT

AIMS: To describe the development process of a device from the conception of the idea to the first contact with the commercial environment, and to demonstrate its practical application through an interdisciplinary collaboration between nursing and engineering for the design of a protective device for peripheral venous catheters. BACKGROUND: Nurses are key agents for identifying unresolved needs or problems related to nursing care. To address these needs, creative ideation processes are often triggered among nurses to seek technological answers to these challenges. RESULTS: The ten steps to develop a device are presented: (1) detecting an unsatisfied clinical need; (2) searching for preexisting marketed products; (3) searching for patents; (4) maintaining confidentiality throughout the process; (5) obtaining institutional support; (6) forming a multidisciplinary team; (7) developing the idea; (8) applying for a patent; (9) building the prototype; (10) marketing the device. This methodology was applied to design a protective device for peripheral venous catheters in hospitalized patients. CONCLUSIONS: Nurses can play a key role in the promotion of healthcare innovation in their field to improve procedures, thanks to their direct contact with patients, and by providing their insight on devices that can enhance patient care. The successful interdisciplinary collaboration between nurses and engineers can provide a response to relevant clinical problems such as the manipulation or removal of peripheral venous catheters. IMPLICATIONS FOR NURSING AND/OR HEALTH POLICY: A hospital policy is required to encourage the participation of nurses in innovative actions. Furthermore, it is important to support nurse leaders who can play a pivotal role in incorporating creativity into work environments and empowering other nurses to innovatively address clinical issues. NO PATIENT OR PUBLIC CONTRIBUTION: This article describes the process for developing a health device.

4.
J Clin Med ; 13(3)2024 Jan 29.
Article in English | MEDLINE | ID: mdl-38337464

ABSTRACT

Previous studies have compared levobupivacaine versus ropivacaine in various peripheral nerve blocks in terms of block duration, quality of analgesia, and onset time, but this has not occurred in the PENG block. Here, a single-center, randomized, and controlled clinical trial is presented. One hundred and twenty patients older than 65 years suffering from hip fractures and surgically treated at our institution under spinal anesthesia were eligible for participation; of them, one hundred and eight were analyzed. Patients were randomized to receive ultrasound-guided PENG blocks using 20 mL of either 0.25% levobupivacaine or 0.375% ropivacaine (both of which are equipotent concentrations). The primary endpoint was to compare the analgesic duration (time to first rescue) and analgesic quality (pain scores using the VAS, PAINAD, and AlgoPlus scales) between the groups. Secondary endpoints included comparing the onset time, describing the need for and type of rescue analgesics, and possible associated adverse effects. There were no statistically significant differences in analgesic duration between levobupivacaine (median 861.0, IQR 960) and ropivacaine (median 1205.0, IQR 1379; p = 0.069). Likewise, the quality of analgesia and onset time were comparable among the groups. A small number of patients required opioids as rescue analgesics (4.6%). The possible associated adverse effects included postoperative infection (11.1%) and delirium (2.8%).

5.
Rev Esp Cardiol (Engl Ed) ; 77(1): 39-49, 2024 Jan.
Article in English, Spanish | MEDLINE | ID: mdl-37217134

ABSTRACT

INTRODUCTION AND OBJECTIVES: We aimed to describe the clinical outcomes of the use of the CentriMag acute circulatory support system as a bridge to emergency heart transplantation (HTx). METHODS: We conducted a descriptive analysis of the clinical outcomes of consecutive HTx candidates included in a multicenter retrospective registry who were treated with the CentriMag device, configured either for left ventricular support (LVS) or biventricular support (BVS). All patients were listed for high-priority HTx. The study assessed the period 2010 to 2020 and involved 16 transplant centers around Spain. We excluded patients treated with isolated right ventricular support or venoarterial extracorporeal membrane oxygenation without LVS. The primary endpoint was 1-year post-HTx survival. RESULTS: The study population comprised 213 emergency HTx candidates bridged on CentriMag LVS and 145 on CentriMag BVS. Overall, 303 (84.6%) patients received a transplant and 53 (14.8%) died without having an organ donor during the index hospitalization. Median time on the device was 15 days, with 66 (18.6%) patients being supported for> 30 days. One-year posttransplant survival was 77.6%. Univariable and multivariable analyses showed no statistically significant differences in pre- or post-HTx survival in patients managed with BVS vs LVS. Patients managed with BVS had higher rates of bleeding, need for transfusion, hemolysis and renal failure than patients managed with LVS, while the latter group showed a higher incidence of ischemic stroke. CONCLUSIONS: In a setting of candidate prioritization with short waiting list times, bridging to HTx with the CentriMag system was feasible and resulted in acceptable on-support and posttransplant outcomes.


Subject(s)
Heart Failure , Heart Transplantation , Heart-Assist Devices , Humans , Heart Failure/epidemiology , Heart Failure/surgery , Retrospective Studies , Heart-Assist Devices/adverse effects , Heart Transplantation/adverse effects , Time Factors , Treatment Outcome
6.
Vox Sang ; 119(1): 27-33, 2024 Jan.
Article in English | MEDLINE | ID: mdl-37986640

ABSTRACT

BACKGROUND AND OBJECTIVES: The efficacy of COVID-19 convalescent plasma (CP) associates with high titres of antibodies. ConPlas-19 clinical trial showed that CP reduces the risk of progression to severe COVID-19 at 28 days. Here, we aim to study ConPlas-19 donors and characteristics that associate with high anti-SARS-CoV-2 antibody levels. MATERIALS AND METHODS: Four-hundred donors were enrolled in ConPlas-19. The presence and titres of anti-SARS-CoV-2 antibodies were evaluated by EUROIMMUN anti-SARS-CoV-2 S1 IgG ELISA. RESULTS: A majority of 80.3% of ConPlas-19 donor candidates had positive EUROIMMUN test results (ratio ≥1.1), and of these, 51.4% had high antibody titres (ratio ≥3.5). Antibody levels decline over time, but nevertheless, out of 37 donors tested for an intended second CP donation, over 90% were still EUROIMMUN positive, and nearly 75% of those with high titres maintained high titres in the second sample. Donors with a greater probability of developing high titres of anti-SARS-CoV-2 antibodies include those older than 40 years of age (RR 2.06; 95% CI 1.24-3.42), with more than 7 days of COVID-19 symptoms (RR 1.89; 95% CI 1.05-3.43) and collected within 4 months from infection (RR 2.61; 95% CI 1.16-5.90). Male donors had a trend towards higher titres compared with women (RR 1.67; 95% CI 0.91-3.06). CONCLUSION: SARS-CoV-2 CP candidate donors' age, duration of COVID-19 symptoms and time from infection to donation associate with the collection of CP with high antibody levels. Beyond COVID-19, these data are relevant to inform decisions to optimize the CP donor selection process in potential future outbreaks.


Subject(s)
COVID-19 , SARS-CoV-2 , Female , Humans , Male , Antibodies, Neutralizing , Antibodies, Viral , Blood Donors , COVID-19/therapy , COVID-19 Serotherapy , Immunization, Passive/methods , Immunoglobulin G , Clinical Trials as Topic
7.
Ann Hematol ; 103(2): 583-591, 2024 Feb.
Article in English | MEDLINE | ID: mdl-37923805

ABSTRACT

Although it is known that increasing age is associated with increased morbidity and mortality in allogeneic transplantation (allo-HSCT), individualization of the process may allow to perform it in progressively older patients.This study analyzed the outcome of 97 patients older than 60 years with a first allo-HSCT performed at our institution between 2011 and 2019.Median age was 66 years (range 60-79) and 15.4% were older than 70 years. The most frequent diagnosis was acute leukemia (50.5%), and 58.8% received a myeloablative conditioning. With a median follow-up of 33.9 months (range 7.9-111.5), at 3-years overall survival (OS) was 50%; progression-free survival (PFS), 46%; cumulative incidence of relapse, 22%; and non-relapse mortality (NRM), 32%. There were no significant differences in OS (p = 0.415), PFS (p = 0.691), cumulative incidence of relapse (p = 0.357) or NRM (p = 0.658) between patients of 60-64 years (n = 37), 65-69 (n = 45) and ≥ 70 years (n = 15). No differences were observed either depending on the intensity of the conditioning regimen in terms of OS (p = 0.858), PFS (p = 0.729), cumulative incidence of relapse (p = 0.416) or NRM (p = 0.270).In conclusion, older adults can safely and effectively undergo allo-HSCT with proper patient selection and individualized transplantation procedures.


Subject(s)
Graft vs Host Disease , Hematopoietic Stem Cell Transplantation , Leukemia, Myeloid, Acute , Humans , Aged , Middle Aged , Feasibility Studies , Retrospective Studies , Leukemia, Myeloid, Acute/complications , Hematopoietic Stem Cell Transplantation/adverse effects , Recurrence , Transplantation Conditioning/methods , Graft vs Host Disease/etiology
8.
Plants (Basel) ; 12(22)2023 Nov 13.
Article in English | MEDLINE | ID: mdl-38005736

ABSTRACT

Anthropogenic disturbance of tropical humid forests leads to habitat loss, biodiversity decline, landscape fragmentation, altered nutrient cycling and carbon sequestration, soil erosion, pest/pathogen outbreaks, among others. Nevertheless, the impact of these alterations in multitrophic interactions, including host-pathogen and vector-pathogen dynamics, is still not well understood in wild plants. This study aimed to provide insights into the main drivers for the incidence of herbivory and plant pathogen damage, specifically, into how vegetation traits at the local and landscape scale modulate such interactions. For this purpose, in the tropical forest of Calakmul (Campeche, Mexico), we characterised the foliar damage caused by herbivores and pathogens in woody vegetation of 13 sampling sites representing a gradient of forest disturbance and fragmentation in an anthropogenic landscape from well preserved to highly disturbed and fragmented areas. We also evaluated how the incidence of such damage was modulated by the vegetation and landscape attributes. We found that the incidence of damage caused by larger, mobile, generalist herbivores, was more sensitive to changes in landscape configuration, while the incidence of damage caused by small and specialised herbivores with low dispersal capacity was more influenced by vegetation and landscape composition. In relation to pathogen symptoms, the herbivore-induced foliar damage seems to be the main factor related to their incidence, indicating the enormous importance of herbivorous insects in the modulation of disease dynamics across tropical vegetation, as they could be acting as vectors and/or facilitating the entry of pathogens by breaking the foliar tissue and the plant defensive barriers. The incidence of pathogen damage also responded to vegetation structure and landscape configuration; the incidence of anthracnose, black spot, and chlorosis, for example, were favoured in sites surrounded by smaller patches and a higher edge density, as well as those with a greater aggregation of semi-evergreen forest patches. Fungal pathogens were shown to be an important cause of foliar damage for many woody species. Our results indicate that an increasing transformation and fragmentation of the tropical forest of southern Mexico could reduce the degree of specialisation in plant-herbivore interactions and enhance the proliferation of generalist herbivores (chewers and scrapers) and of mobile leaf suckers, and consequently, the proliferation of some symptoms associated with fungal pathogens such as fungus black spots and anthracnose. The symptoms associated with viral and bacterial diseases and to nutrient deficiency, such as chlorosis, could also increase in the vegetation in fragmented landscapes with important consequences in the health and productivity of wild and cultivated plant species. This is a pioneering study evaluating the effect of disturbances on multitrophic interactions, offering key insights on the main drivers of the changes in herbivory interactions and incidence of plant pathogens in tropical forests.

9.
JMIR Med Inform ; 11: e45315, 2023 Sep 19.
Article in English | MEDLINE | ID: mdl-37787663

ABSTRACT

Background: The creation of computer-supported collaborative clinical cases is an area of educational research that has been widely studied. However, the reuse of cases and their sharing with other platforms is a problem, as it encapsulates knowledge in isolated platforms without interoperability. This paper proposed a workflow ecosystem for the collaborative design and distribution of clinical cases through web-based computing platforms that (1) allow medical students to create clinical cases collaboratively in a dedicated environment; (2) make it possible to export these clinical cases in terms of the Health Level 7 (HL7) Fast Healthcare Interoperability Resources (FHIR) interoperability standard; (3) provide support to transform imported cases into learning object repositories; and (4) use e-learning standards (eg, Instructional Management Systems Content Packaging [IMS-CP] or Sharable Content Object Reference Model [SCORM]) to incorporate this content into widely-used learning management systems (LMSs), letting medical students democratize a valuable knowledge that would otherwise be confined within proprietary platforms. Objective: This study aimed to demonstrate the feasibility of developing a workflow ecosystem based on IT platforms to enable the collaborative creation, export, and deployment of clinical cases. Methods: The ecosystem infrastructure for computer-supported collaborative design of standardized clinical cases consists of three platforms: (1) Mosaico, a platform used in the design of clinical cases; (2) Clavy, a tool for the flexible management of learning object repositories, which is used to orchestrate the transformation and processing of these clinical cases; and (3) Moodle, an LMS that is geared toward publishing the processed clinical cases and delivering their course deployment stages in IMS-CP or SCORM format. The generation of cases in Mosaico is exported in the HL7 FHIR interoperability standard to Clavy, which is then responsible for creating and deploying a learning object in Moodle. Results: The main result was an interoperable ecosystem that demonstrates the feasibility of automating the stages of collaborative clinical case creation, export through HL7 FHIR standards, and deployment in an LMS. This ecosystem enables the generation of IMS-CPs associated with the original Mosaico clinical cases that can be deployed in conventional third-party LMSs, thus allowing the democratization and sharing of clinical cases to different platforms in standard and interoperable formats. Conclusions: In this paper, we proposed, implemented, and demonstrated the feasibility of developing a standards-based workflow that interoperates multiple platforms with heterogeneous technologies to create, transform, and deploy clinical cases on the web. This achieves the objective of transforming the created cases into a platform for web-based deployment in an LMS.

10.
Sensors (Basel) ; 23(19)2023 Sep 28.
Article in English | MEDLINE | ID: mdl-37836987

ABSTRACT

In this paper, a novel concept of a three-dimensional full metal system including a Dual-Mode Converter (DMC) network integrated with a high-gain Conical Horn Antenna (CHA) is presented. This system is designed for 5G millimeter wave applications requiring monopulse operation at K-band (37.5-39 GHz). The DMC integrates two mode converters. They excite either the TE11cir or the TE01cir modes of the circular waveguide of the CHA. The input of the mode converters is the TE10rec mode of two independent WR-28 standard rectangular waveguide ports. By integrating the DMC with the CHA, the whole system, called a Dual-Mode Conical Horn Antenna (DM-CHA), is formed, radiating the sum (Σ) and difference (Δ) patterns associated to the monopulse operation. To adequately prevent the propagation of higher order modes and mode mutual coupling, this integration procedure is carefully designed and fabricated. To prove the performance of the design, the DMC network was fabricated using subtractive manufacturing by Computer Numerical Control (CNC) technology. The CHA was fabricated using additive manufacturing by Direct Metal Laser Sintering (DLMS) technology. Finally, the simulation and measurement results were exhaustively compared, including return loss, isolation, radiation pattern, and gain of the full DM-CHA structure. It is noteworthy that this system provided up to ±11° per beam in the angular of arrival detection to support the high data rate operation for 5G satellite communications in the millimeter-wave band.

11.
Toxics ; 11(10)2023 Oct 05.
Article in English | MEDLINE | ID: mdl-37888690

ABSTRACT

Triazole fungicides are widely used in the world, mainly in agriculture, but their abuse and possible toxic effects are being reported in some in vivo and in vitro studies that have demonstrated their danger to human health. This in vitro study evaluated the cytotoxicity, oxidative stress and proinflammation of EA.hy926 endothelial cells in response to ipconazole exposure. Using the MTT assay, ipconazole was found to produce a dose-dependent reduction (*** p < 0.001; concentrations of 20, 50 and 100 µM) of cell viability in EA.hy926 with an IC50 of 29 µM. Also, ipconazole induced a significant increase in ROS generation (** p < 0.01), caspase 3/7 (** p < 0.01), cell death (BAX, APAF1, BNIP3, CASP3 and AKT1) and proinflammatory (NLRP3, CASP1, IL1ß, NFκB, IL6 and TNFα) biomarkers, as well as a reduction in antioxidant (NRF2 and GPx) biomarkers. These results demonstrated that oxidative stress, proinflammatory activity and cell death could be responsible for the cytotoxic effect produced by the fungicide ipconazole, such that this triazole compound should be considered as a possible risk factor in the development of alterations in cellular homeostasis.

12.
J Cancer Res Clin Oncol ; 149(16): 15085-15090, 2023 Nov.
Article in English | MEDLINE | ID: mdl-37615820

ABSTRACT

PURPOSE: Our study aims to determine whether there are differences in the degree of detection of prostate cancer (PCa) and CsPCa between fusion prostate biopsy (FPB), cognitive biopsy (PCB), and randomized, systematic biopsy (SB). METHODS: A retrospective analysis was carried out of 195 patients with suspected PCa at the San Cecilio University Clinical Hospital in Granada who underwent a prostate biopsy between January and December 2021. Patients were divided into three groups: group 1, patients undergoing FPB transperineally with ultrasound BK 3000 (N = 87); group 2, PCB (N = 59) transperineally; and group 3, transrectal SB (N = 49), the latter two, with an ultrasound BK Specto. RESULTS: We found differences in favor of image-directed biopsies (FPB and PCB) with a percentage of positive biopsies of 52.8% and 50%, respectively, compared to 41.4% with SB, but without these differences being significant. Given the controversy in performing prostate biopsies in PI-RADS 3 lesions reported in the literature, a subanalysis was performed excluding the FPB performed for PI-RADS 3 lesions (PI-RADS 4 and 5 are included), finding significant differences when comparing FPB with PCB and SB (group 1, 64% vs group 2, 45.8%; p = 0.05) (group 1, 64% vs group 3, 42.9%; p = 0.035). CONCLUSION: With the results obtained in our series, we conclude that the finding of a PI-RADS 3 lesion in mpMRI should not be an absolute criterion to indicate prostate biopsy. On the other hand, for PI-RADS 4 and 5 lesions, FPB is recommended, which in this case turns out to be superior to PCB and SB.


Subject(s)
Prostate , Prostatic Neoplasms , Male , Humans , Prostate/diagnostic imaging , Prostate/pathology , Prostatic Neoplasms/diagnostic imaging , Prostatic Neoplasms/pathology , Magnetic Resonance Imaging/methods , Retrospective Studies , Biopsy , Image-Guided Biopsy/methods , Cognition
13.
Toxics ; 11(7)2023 Jun 30.
Article in English | MEDLINE | ID: mdl-37505534

ABSTRACT

Ipconazole is an antifungal agrochemical widely used in agriculture against seed diseases of rice, vegetables, and other crops; due to its easy accumulation in the environment, it poses a hazard to human, animal, and environmental health. Therefore, we investigated the cytotoxic effect of ipconazole on SH-SY5Y neuroblastoma cells using cell viability tests (MTT), ROS production, caspase3/7 activity, and molecular assays of the biomarkers of cell death (Bax, Casp3, APAF1, BNIP3, and Bcl2); inflammasome (NLRP3, Casp1, and IL1ß); inflammation (NFκB, TNFα, and IL6); and antioxidants (NRF2, SOD, and GPx). SH-SY5Y cells were exposed to ipconazole (1, 5, 10, 20, 50, and 100 µM) for 24 h. The ipconazole, in a dose-dependent manner, reduced cell viability and produced an IC50 of 32.3 µM; it also produced an increase in ROS production and caspase3/7 enzyme activity in SH-SY5Y cells. In addition, ipconazole at 50 µM induced an overexpression of Bax, Casp3, APAF1, and BNIP3 (cell death genes); NLRP3, Casp1, and IL1B (inflammasome complex genes); and NFκB, TNFα, and IL6 (inflammation genes); it also reduced the expression of NRF2, SOD, and GPx (antioxidant genes). Our results show that ipconazole produces cytotoxic effects by reducing cell viability, generating oxidative stress, and inducing cell death in SH-SY5Y cells, so ipconazole exposure should be considered as a factor in the presentation of neurotoxicity or neurodegeneration.

14.
Molecules ; 28(11)2023 May 25.
Article in English | MEDLINE | ID: mdl-37298802

ABSTRACT

An important biomarker of oxidative damage in cellular DNA is the formation of 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-oxodG). Although several methods are available for the biochemical analysis of this molecule, its determination at the single cell level may provide significant advantages when investigating the influence of cell heterogeneity and cell type in the DNA damage response. to. For this purpose, antibodies recognizing 8-oxodG are available; however, detection with the glycoprotein avidin has also been proposed because of a structural similarity between its natural ligand biotin and 8-oxodG. Whether the two procedures are equivalent in terms of reliability and sensitivity is not clear. In this study, we compared the immunofluorescence determination of 8-oxodG in cellular DNA using the monoclonal antibody N45.1 and labeling using avidin conjugated with the fluorochrome Alexa Fluor488 (AF488). Oxidative DNA damage was induced in different cell types by treatment with potassium bromate (KBrO3), a chemical inducer of reactive oxygen species (ROS). By using increasing concentrations of KBrO3, as well as different reaction conditions, our results indicate that the monoclonal antibody N45.1 provides a specificity of 8-oxodG labeling greater than that attained with avidin-AF488. These findings suggest that immunofluorescence techniques are best suited to the in situ analysis of 8-oxodG as a biomarker of oxidative DNA damage.


Subject(s)
Avidin , Deoxyguanosine , 8-Hydroxy-2'-Deoxyguanosine/metabolism , Deoxyguanosine/metabolism , Reproducibility of Results , DNA Damage , Oxidative Stress , Biomarkers/metabolism , Antibodies, Monoclonal/metabolism , DNA/chemistry
15.
Front Neuroinform ; 17: 1092967, 2023.
Article in English | MEDLINE | ID: mdl-36938360

ABSTRACT

Nowadays, an enormous amount of high dimensional data is available in the field of neuroscience. Handling these data is complex and requires the use of efficient tools to transform them into useful knowledge. In this work we present NeuroSuites, an easy-access web platform with its own architecture. We compare our platform with other software currently available, highlighting its main strengths. Thanks to its defined architecture, it is able to handle large-scale problems common in some neuroscience fields. NeuroSuites has different neuroscience-oriented applications and tools to integrate statistical data analysis and machine learning algorithms commonly used in this field. As future work, we want to further expand the list of available software tools as well as improve the platform interface according to user demands.

17.
Nutrients ; 15(3)2023 Feb 01.
Article in English | MEDLINE | ID: mdl-36771451

ABSTRACT

It has been proposed that oxidative stress is a pathogenic mechanism to induce cytotoxicity and to cause cardiovascular and neuronal diseases. At present, natural compounds such as plant extracts have been used to reduce the cytotoxic effects produced by agents that induce oxidative stress. Our study aimed to evaluate the antioxidant and cytoprotective capacity of Desmodium tortuosum (D. tortuosum) extract in the co- and pre-treatment in EA.hy926 and SH-SY5Y cell lines subjected to oxidative stress induced by tert-butylhydroperoxide (t-BOOH). Cell viability, reactive oxygen species (ROS), nitric oxide (NO), caspase 3/7 activity, reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GR), and molecular expression of oxidative stress biomarkers (SOD2, NRF2 and NFκB1) and cell death (APAF1, BAX, Caspase3) were all evaluated. It was observed that the D. tortuosum extract, in a dose-dependent manner, was able to reduce the oxidative and cytotoxicity effects induced by t-BOOH, even normalized to a dose of 200 µg/mL, which would be due to the high content of phenolic compounds mainly phenolic acids, flavonoids, carotenoids and other antioxidant compounds. Finally, these results are indicators that the extract of D. tortuosum could be a natural alternative against the cytotoxic exposure to stressful and cytotoxic chemical agents.


Subject(s)
Fabaceae , Neuroblastoma , Humans , Antioxidants/pharmacology , Antioxidants/metabolism , Fabaceae/chemistry , Oxidative Stress , Reactive Oxygen Species/metabolism , Glutathione/metabolism , Plant Extracts/pharmacology , Plant Extracts/chemistry , South America
18.
Trop Anim Health Prod ; 55(2): 66, 2023 Feb 04.
Article in English | MEDLINE | ID: mdl-36738381

ABSTRACT

Sarcocystis masoni n. sp. (known as "S. lamacanis") infects alpacas affecting their productivity and can cause a food poisoning syndrome in humans by consuming contaminated, undercooked cardiac muscle. There are few studies estimating the prevalence of this parasite in alpacas, although this information is crucial for the control and prevention of sarcocystosis. This study aimed to determine the frequency and density of Sarcocystis masoni n. sp. in the heart of alpacas in Huancavelica, a province of the Andean region of Peru. Heart samples were taken for histopathology from 104 alpacas slaughtered at the municipal slaughterhouse of Huancavelica, the official abattoir in the Huancavelica district. No macroscopic sarcocysts were observed. All alpacas (100%) had microscopic sarcocysts of Sarcocystis masoni n. sp., with no inflammatory reactions. The alpacas showed an average sarcocyst density of 60.8 ± 23.3/mm2. Sarcocysts density was significantly higher (p < 0.05) as the age of the animals increased. In addition, sarcocysts density was significantly higher (p < 0.05) in male animals aged 4 and 5 years compared to females of the same age. These results confirmed that heart sarcocystosis is highly endemic in Peruvian alpacas. Therefore, it is recommended that alpaca hearts be well-cooked at the time of consumption. The present study showed current data and contributes to the knowledge of this parasitosis. Studies of this nature are necessary because they are the basis for developing animal health programs.


Subject(s)
Camelids, New World , Sarcocystis , Sarcocystosis , Humans , Female , Male , Animals , Sarcocystosis/epidemiology , Sarcocystosis/veterinary , Camelids, New World/parasitology , Peru/epidemiology , Phylogeny , Myocardium , Risk Factors
19.
Cir. Esp. (Ed. impr.) ; 101(1): 29-34, en. 2023. tab
Article in Spanish | IBECS | ID: ibc-226684

ABSTRACT

Introducción: La pandemia de COVID-19 ha afectado a las oportunidades de formación de los profesionales sanitarios, en parte porque se anularon muchos cursos presenciales. En este estudio se analizan los resultados de participación y satisfacción de las sesiones del Aula Virtual AEC durante su primer año. Métodos: El Aula Virtual AEC incluye un formato combinado de seminarios semanales emitidos en directo que pueden ser visionados en diferido. En este estudio se evalúan los resultados en sus primeros 12 meses, considerando el número de participantes en directo, el número de visualizaciones en diferido y el alcance global, así como los resultados de la encuesta de satisfacción realizada en cada una de las sesiones (1-10) Resultados: Desde el 16 de abril de 2020 hasta el 15 de abril de 2021 se realizaron 50 sesiones del Aula Virtual AEC. El alcance medio de las sesiones ha sido de 509 ± 288 visualizaciones con un rango entre 196 y 1490. En los picos de la pandemia se observó un descenso de los participantes en directo: 275 ± 135 vs. 391 ± 233 (p = 0,032) La puntuación media sobre el formato fue 8,46 ± 0,31/10. Las sesiones mejor puntuadas fueron las de temática relacionada con coloproctología con una diferencia estadísticamente significativa en la puntuación media 8,79 ± 0,42 vs. 8,39 ± 0,27 (p = 0,035). Un 90,76% de usuarios consideraron las sesiones útiles. Un 97,2% consideraban que deben mantenerse tras la pandemia. Conclusiones: El Aula Virtual AEC ha tenido muy buenos resultados en los primeros 12 meses de desarrollo, resultando ser una herramienta útil de docencia quirúrgica que previsiblemente sobrevivirá a la época de pandemia. (AU)


Introduction: The SARS-CoV-2 pandemic has affected training opportunities for healthcare professionals partly because face to face courses were cancelled. This study analyzes the results of participation and satisfaction of the AEC Virtual Classroom sessions during the first year. Methods: The AEC Virtual Classroom includes a combined format of weekly Webinar broadcast live that can be viewed on a delayed basis in a virtual platform. In this study, the results in its first year are evaluated considering the number of live participants, the delayed views and the global reach; as well as the results of the satisfaction survey in each of the sessions (0–10). Results: From 16/04/2020 to 15/04/2021, 50 sessions of the Virtual Classroom AEC were held. The average scope of the sessions was 509 ± 288 views with a range between 196 and 149. At the times of highest incidence of cases during the pandemic, a decrease in live participants was observed 275 ± 135 vs. 391 ± 233 (p = 0.032). The mean score on the format was 8.46 ± 0.31/10. The best-scored sessions were those of the subject related to coloproctology with a statistically significant difference in the mean score 8.79 ± 0.42 vs. 8.39 ± 0.27 (p = 0.035). 90% of users considered the sessions useful. 97.2% of respondents believe that the sessions should be maintained after the pandemic. Conclusions: The AEC Virtual Classroom has a very good results in the first year, proving to be a useful surgical teaching tool that will foreseeably survive once the pandemic is over. (AU)


Subject(s)
Humans , Pandemics , Coronavirus Infections/epidemiology , Education/trends , Education, Distance
20.
Cir. Esp. (Ed. impr.) ; 101(1): 29-34, en. 2023. tab
Article in Spanish | IBECS | ID: ibc-EMG-424

ABSTRACT

Introducción: La pandemia de COVID-19 ha afectado a las oportunidades de formación de los profesionales sanitarios, en parte porque se anularon muchos cursos presenciales. En este estudio se analizan los resultados de participación y satisfacción de las sesiones del Aula Virtual AEC durante su primer año. Métodos: El Aula Virtual AEC incluye un formato combinado de seminarios semanales emitidos en directo que pueden ser visionados en diferido. En este estudio se evalúan los resultados en sus primeros 12 meses, considerando el número de participantes en directo, el número de visualizaciones en diferido y el alcance global, así como los resultados de la encuesta de satisfacción realizada en cada una de las sesiones (1-10) Resultados: Desde el 16 de abril de 2020 hasta el 15 de abril de 2021 se realizaron 50 sesiones del Aula Virtual AEC. El alcance medio de las sesiones ha sido de 509 ± 288 visualizaciones con un rango entre 196 y 1490. En los picos de la pandemia se observó un descenso de los participantes en directo: 275 ± 135 vs. 391 ± 233 (p = 0,032) La puntuación media sobre el formato fue 8,46 ± 0,31/10. Las sesiones mejor puntuadas fueron las de temática relacionada con coloproctología con una diferencia estadísticamente significativa en la puntuación media 8,79 ± 0,42 vs. 8,39 ± 0,27 (p = 0,035). Un 90,76% de usuarios consideraron las sesiones útiles. Un 97,2% consideraban que deben mantenerse tras la pandemia. Conclusiones: El Aula Virtual AEC ha tenido muy buenos resultados en los primeros 12 meses de desarrollo, resultando ser una herramienta útil de docencia quirúrgica que previsiblemente sobrevivirá a la época de pandemia. (AU)


Introduction: The SARS-CoV-2 pandemic has affected training opportunities for healthcare professionals partly because face to face courses were cancelled. This study analyzes the results of participation and satisfaction of the AEC Virtual Classroom sessions during the first year. Methods: The AEC Virtual Classroom includes a combined format of weekly Webinar broadcast live that can be viewed on a delayed basis in a virtual platform. In this study, the results in its first year are evaluated considering the number of live participants, the delayed views and the global reach; as well as the results of the satisfaction survey in each of the sessions (0–10). Results: From 16/04/2020 to 15/04/2021, 50 sessions of the Virtual Classroom AEC were held. The average scope of the sessions was 509 ± 288 views with a range between 196 and 149. At the times of highest incidence of cases during the pandemic, a decrease in live participants was observed 275 ± 135 vs. 391 ± 233 (p = 0.032). The mean score on the format was 8.46 ± 0.31/10. The best-scored sessions were those of the subject related to coloproctology with a statistically significant difference in the mean score 8.79 ± 0.42 vs. 8.39 ± 0.27 (p = 0.035). 90% of users considered the sessions useful. 97.2% of respondents believe that the sessions should be maintained after the pandemic. Conclusions: The AEC Virtual Classroom has a very good results in the first year, proving to be a useful surgical teaching tool that will foreseeably survive once the pandemic is over. (AU)


Subject(s)
Humans , Pandemics , Coronavirus Infections/epidemiology , Education/trends , Severe acute respiratory syndrome-related coronavirus , Education, Distance
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