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1.
Comp Immunol Microbiol Infect Dis ; 107: 102138, 2024 Apr.
Article in English | MEDLINE | ID: mdl-38367297

ABSTRACT

Leptospirosis is a bacterial zoonosis that affects both humans and animals worldwide. Currently, it is known that cats may be susceptible to infection. This study aims to investigate the presence of anti-Leptospira spp. antibodies and leptospiruria in cats, using Microscopic Agglutination Test (MAT) and Real-time Polymerase Chain Reaction (PCR) techniques, respectively. A total of 76 cats, undergoing comprehensive anamnesis, general physical examination, and complementary exams were included in the investigation. Among the 76 cats tested, 9.2% (7/76) exhibited the presence of anti-Leptospira spp. antibodies, while Leptospira spp. DNA was detected in at 1.3% (1/76) of the evaluated urine samples. No significant associations were observed between the serological and molecular diagnostic results and the assessed variables, including clinical data and laboratory results of cats testing positive. This study provides insight into the occurrence of Leptospira spp. infection and leptospiruria in cats treated at a veterinary teaching hospital in southern Brazil.


Subject(s)
Leptospira , Leptospirosis , Humans , Cats , Animals , Leptospira/genetics , Hospitals, Animal , Brazil/epidemiology , Hospitals, Teaching , Leptospirosis/diagnosis , Leptospirosis/epidemiology , Leptospirosis/veterinary , Agglutination Tests/veterinary , Antibodies, Bacterial
2.
JBI Evid Implement ; 21(4): 409-431, 2023 Dec 01.
Article in English | MEDLINE | ID: mdl-37975298

ABSTRACT

INTRODUCTION: Facilitation is a key element of evidence implementation. Although quantitative systematic reviews have been undertaken to examine its components and effectiveness, no attempt has been made to synthesize qualitative evidence examining the experiences of facilitators on how facilitation is operationalized, the challenges associated with it, and the factors that can influence its perceived effectiveness. METHODS: A systematic review of qualitative studies was conducted using the JBI methodology. RESULTS: A total of 36 qualitative studies was included in the systematic review, with the majority being assessed as high quality following critical appraisal. The findings were extracted and further synthesized, highlighting that facilitation involves providing technical and non-technical support to health professionals, as well as high-intensity collaborations and relationship building. Determinants of perceived effectiveness of facilitation include facilitators' access to resources and learning support; their skills, traits/attitudes, and approach to facilitation; and the context of the organization where the implementation occurs. Work demands, emotional stress, and lack of clarity in roles and career development can pose challenges for facilitators. CONCLUSION: To maximize the outcomes of facilitation in evidence implementation, the team of facilitators should be carefully selected to ensure they have the right skills, traits/attitudes, and approach to facilitation. They should also be provided with dedicated time to conduct the facilitation and have access to resources, training, and mentoring support. Future research should aim to examine the perspectives of the "implementers" who received support from facilitators to gain a better understanding of which facilitation strategies have an impact on clinical practice behavior. REVIEW REGISTRATION NUMBER: PROSPERO CRD42023402496.


Subject(s)
Attitude , Health Personnel , Humans , Health Personnel/education , Learning , Qualitative Research , Mentors
3.
Front Public Health ; 11: 1224332, 2023.
Article in English | MEDLINE | ID: mdl-37780429

ABSTRACT

Background: A large number of workers attend work despite being ill. Attending work during sickness can have a number of consequences for the worker (e.g., worsening of physical and mental condition), for co-workers, and for the company, and for service users. Objectives: The aim of this study was to assess the factors influencing presenteeism and mental health of workers during the COVID-19 pandemic. Methods: A systematic review following the PRISMA format was conducted in the PubMed, Scopus, Web of Science (WoS), Cumulative Index to Nursing and Allied Health Literature (CINAHL), PsycInfo, and ScienceDirect electronic databases in January 2023, using the following key words: Presenteeism, Mental Health, and COVID-19. The eligibility criteria applied were original articles published in English, Spanish, French, German, and Portuguese, workers during the COVID-19 pandemic (data collection date: January 01, 2020 - January 01, 2023), and articles assessing at least one measure of presenteeism and mental health status. Methodological quality was assessed using the critical appraisal tools of the Joanna Briggs Institute. The followed protocol is listed in the International Prospective Register of Systematic Reviews (PROSPERO) with code CRD42023391409. Results: A total of 25 studies were included in this review recruiting a total of 164,274 participants. A number of factors influencing mental health and sickness presenteeism were identified: (1) mental health-related factors (burnout [in 4 studies], stress [in 9 studies], depression [in 1 study], fear of COVID-19 [in 1 study], no well-being [in 2 studies], etc.); (2) individual factors (health status [in 1 study], being young [in 1 study], workers who experienced interrupted medical care [in 2 studies], having a chronic disease [in 1 study], etc.); (3) factors related to the situation caused by COVID-19 (confinement, symptoms, loss of contract, risk of bankruptcy, etc. [in 1 study each one]); and (4) factors derived from working conditions (organisational support [in 1 study], patient care [in 1 study], work functioning or task performance impairment [in 4 studies], work fatigue [in 2 studies], safety climate [in 1 study], workload [in 1 study], etc.). Conclusion: Identifying the key determinants of presenteeism and understanding the phenomena and origins of sickness presenteeism will help to create a safe working environment and optimal organisational systems to protect vulnerable workers in a pandemic context. Systematic review registration: The unique identifier is CRD42023391409.


Subject(s)
COVID-19 , Humans , COVID-19/epidemiology , Mental Health , Pandemics , Presenteeism , Workload
4.
Comp Immunol Microbiol Infect Dis ; 102: 102063, 2023 Nov.
Article in English | MEDLINE | ID: mdl-37757627

ABSTRACT

Surveillance of bovine tuberculosis (bTB) lesions in animals at slaughterhouses is useful for controlling and eradicating the disease, besides providing epidemiological information. This study aimed to identify risk factors for bovine tuberculosis (bTB) condemnation in cattle at slaughterhouses in Rio Grande do Sul, Brazil. A logistic regression analysis was conducted using data on bTB-related condemnations. Variables examined included animal origin, number of slaughtered animals, season, inspection level (state or municipality), animal sex, and slaughterhouse location. A total of 297,817 Animal Transport Guides were evaluated, representing the transportation of 3497,521 animals. Among these, 6097 (2.05%) had at least one animal condemned due to bTB lesions. Risk factors for condemnation included larger batch sizes, female animals, slaughterhouses, and animal origin. The higher condemnation frequency in females and regions with dairy farms suggests links to milk production. Variation in condemnation rates by inspection level and slaughterhouse highlights the need for standardized procedures in identifying bTB lesions. Identifying these risk factors enables targeted interventions to enhance disease control and eradication efforts.


Subject(s)
Cattle Diseases , Tuberculosis, Bovine , Tuberculosis , Cattle , Female , Animals , Tuberculosis, Bovine/epidemiology , Brazil/epidemiology , Tuberculosis/veterinary , Risk Factors , Abattoirs , Cattle Diseases/epidemiology
5.
JBI Evid Synth ; 21(12): 2422-2428, 2023 Dec 01.
Article in English | MEDLINE | ID: mdl-37747432

ABSTRACT

OBJECTIVE: This systematic review will assess the effectiveness of school-based sleep promotion programs on sleep knowledge, hygiene, quality, behavior, and efficiency in adolescents. INTRODUCTION: Adolescents need to sleep between 8 and 10 hours per night; however, most sleep fewer hours due to peer and school pressure, stress, and excessive use of electronic devices at night. Sleep deprivation can lead to cardiovascular problems, issues with stress management, and suicidal ideation. Schools are an ideal space to implement intervention programs, providing the opportunity to reach a large number of adolescents. Considering the negative impacts of poor sleep, it is essential to assess the effectiveness of school-based sleep promotion programs on sleep knowledge, hygiene, quality, behavior, and efficiency. INCLUSION CRITERIA: Studies with adolescents (10-19 years old) exposed to school-based sleep promotion programs will be included. Studies in English, Spanish, and Portuguese will be considered, with no time limits. METHODS: Nine databases, CINAHL, Cochrane Central Register of Controlled Trials, ERIC (all via EBSCOhost), MEDLINE (PubMed), LILACS, SciELO, Psychology and Behavioral Sciences Collection, Web of Science Core Collection, and Scopus, will be searched from inception to identify relevant studies. Two independent reviewers will perform the study selection, critical appraisal using JBI tools, and data extraction. Studies will, where possible, be pooled in meta-analysis. Heterogeneity will be assessed using the standard χ 2 and I2 tests. Statistical analyses will be performed using the random effects model. If fewer than 5 studies are included, the fixed effects model will be used. The Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach will be used to grade the certainty of evidence and a Summary of Findings will be created. REVIEW REGISTRATION: PROSPERO CRD42023374838.


Subject(s)
Sleep , Humans , Adolescent , Child , Young Adult , Adult , Systematic Reviews as Topic , Meta-Analysis as Topic
6.
JBI Evid Implement ; 2023 Jul 22.
Article in English | MEDLINE | ID: mdl-37477193

ABSTRACT

INTRODUCTION AND OBJECTIVES: Sexually transmitted infections are a public health problem affecting 45% of adolescents and young adults worldwide. The evidence suggests that primary care settings are uniquely positioned to provide an opportunity for these preventive interventions. The aim of this project is to improve nurses' interventions for preventing risky sexual behaviors in adolescents attending nursing consultations in a primary healthcare unit. METHODS: An audit and feedback were conducted by the JBI Model and Implementation Framework. Five audit criteria representing best practice recommendations for preventing risky sexual behaviors in adolescents were used. Barriers to compliance with the best practices were identified, and strategies were adopted to overcome them. A follow-up audit was conducted using the same approach as the baseline audit. RESULTS: Compliance rates improved in four criteria from baseline audit to follow-up audit. CONCLUSION: Through auditing and feedback, evidence-based interventions were implemented to prevent sexual risk behavior in adolescents in primary care settings. Further best practice implementation projects should be conducted to improve adolescent health outcomes.

7.
Am J Primatol ; 85(4): e23472, 2023 04.
Article in English | MEDLINE | ID: mdl-36814095

ABSTRACT

The environments in which neotropical primates live have been undergoing an intense fragmentation process, constituting a major threat to the species' survival and causing resource scarcity, social isolation, and difficulty in dispersal, leaving populations increasingly vulnerable. Moreover, the proximity of wild environments to anthropized landscapes can change the dynamics of pathogens and the parasite-host-environment relationship, creating conditions that favor exposure to different pathogens. To investigate the previous exposure of free-living primates in Rio Grande do Sul State (RS), southern Brazil, to the bacterial agents Leptospira spp. and Brucella abortus, we investigated agglutinating antibodies against 23 serovars of Leptospira spp. using the microscopic agglutination test and B. abortus acidified antigen test in primate serum samples; 101 samples from primates captured between 2002 and 2016 in different forest fragments were used: 63 Alouatta caraya, 36 Alouatta guariba clamitans, and 02 Sapajus nigritus cucullatus. In addition, the forest remnants where the primates were sampled were characterized in a multiscale approach in radii ranging from 200 to 1400 m to investigate the potential relationship of previous exposure to the agent with the elements that make up the landscape structure. The serological investigation indicated the presence of antibodies for at least one of the 23 serovars of Leptospira spp. in 36.6% (37/101) of the samples analyzed, with titers ranging from 100 to 1600. The most observed serovars were Panama (17.8%), Ballum (5.9%), Butembo (5.9%), Canicola (5.9%), Hardjo (4.9%), and Tarassovi (3.9%); no samples were seropositive for Brucella abortus. Decreased forest cover and edge density were the landscape factors that had a significant relationship with Leptospira spp. exposure, indicating that habitat fragmentation may influence contact with the pathogen. The data generated in this study demonstrate the importance of understanding how changes in landscape structure affect exposure to pathogenic microorganisms of zoonotic relevance. Hence, improving epidemiological research and understanding primates' ecological role in these settings can help improve environmental surveillance and conservation strategies for primate populations in different landscapes.


Subject(s)
Alouatta caraya , Brucellosis , Cebinae , Leptospira , Leptospirosis , Animals , Brucella abortus , Leptospirosis/epidemiology , Leptospirosis/veterinary , Brucellosis/epidemiology , Brucellosis/veterinary , Brucellosis/microbiology , Brazil/epidemiology , Antibodies, Bacterial
8.
Nurs Rep ; 13(1): 148-156, 2023 Jan 31.
Article in English | MEDLINE | ID: mdl-36810267

ABSTRACT

Oral hygiene has been shown to reduce adverse events and promote the quality of life of patients with stroke. However, a stroke can result in the impairment of physical, sensory, and cognitive abilities, and comprise self-care. Although nurses recognize its benefits, there are areas for improvement in the implementation of the best evidence-based recommendations. The aim is to promote compliance with the best evidence-based recommendations on oral hygiene in patients with stroke. This project will follow the JBI Evidence Implementation approach. The JBI Practical Application of Clinical Evidence System (JBI PACES) and the Getting Research into Practice (GRiP) audit and feedback tool will be used. The implementation process will be divided into three phases: (i) establishing a project team and undertaking the baseline audit; (ii) providing feedback to the healthcare team, identifying barriers to the implementation of best practices, and co-designing and implementing strategies using GRIP, and (iii) undertaking a follow-up audit to assess the outcomes and plan for sustainability. So, the successful adoption of the best evidence-based recommendations on oral hygiene in patients with stroke will reduce the adverse events related to poor oral care and may improve patients' quality of care. This implementation project has great transferability potential to other contexts.

9.
Trab. Educ. Saúde (Online) ; 21: e02338223, 2023.
Article in Portuguese | LILACS | ID: biblio-1450604

ABSTRACT

RESUMO O objetivo desta nota é colocar em evidência alguns apontamentos para se pensar a educação escolar em tempo de pós-pandemia. As interfaces entre o processo formativo do sujeito e a educação escolar em tempo de pós-pandemia se encontram em um paradoxo: por um lado, a dificuldade na construção, pelo viés democrático, da proposta de ensino da unidade escolar como lugar para se fazer o espaço do comum, na partilha da cultura escolar; por outro lado, a exclusão que se encontra instituída no sistema educacional e que restringe o direito à educação.


ABSTRACT The aim of this article is to highlight some notes to think about school education in post-pandemic times. The interfaces between the subject's formative process and school education in post-pandemic times are in a paradox: on the one hand, the difficulty building, through democratic bias, the proposal of teaching the school unit as a place to make the space of the common, in sharing the school culture; on the other hand, the exclusion that is established in the educational system and that restricts the right to education.


RESUMEN El objetivo de esta nota es poner en evidencia algunos apuntes para pensar en la educación escolar en el tiempo post-pandemia. Las interfaces entre el proceso formativo del sujeto y la educación escolar en tiempo de post-pandemia se encuentran en una paradoja: por un lado, la dificultad de construir, por el sesgo democrático, la propuesta de enseñar la unidad escolar como lugar para hacer el espacio de lo común, compartir la cultura escolar; por otra parte, la exclusión que se ha establecido en el sistema educativo y que limita el derecho a la educación.


Subject(s)
Education , Pandemics , COVID-19
10.
JBI Evid Implement ; 20(3): 180-188, 2022 Sep 01.
Article in English | MEDLINE | ID: mdl-36373356

ABSTRACT

BACKGROUND: Facilitation is a key component of JBI's approach to evidence implementation along with context analysis and evaluation of process and outcomes. Although the role of facilitation is recognized as a critical component of evidence implementation, what constitutes effective facilitation is poorly understood. AIM: This article presents a descriptive exploration of facilitation as it occurs in evidence implementation initiatives conducted in various healthcare and geographical contexts. All projects used the JBI approach to evidence implementation. METHODS: To provide a multinational perspective on how facilitation was operationalized to promote positive changes in clinical practice and health outcomes, five case studies of evidence implementation projects are presented. RESULTS: The cases highlighted that facilitation is a multifaceted process that can be met through a variety of roles that address aspects of education and capacity building, partnerships, action planning, problem solving and evaluation. Facilitation in all cases appeared to be collaborative, with multiple 'players' within and outside of the health organization being involved in the process. Although there are similarities in activities, facilitation involved some level of local contextualization where there were unique or additional activities performed to accommodate the local needs and requirements of the health organization involved in each case. Numerous contextual factors influenced the success of the implementation initiative. CONCLUSION: The cases emphasized the complex nature of facilitation as a strategy for evidence implementation, indicating that contextual attributes and features define the range of knowledge, skills, and activities that should take place in order for facilitation to be effective. Although there appears to be some core components, tailoring and adaptation of the facilitation process (or roles) is required.


Subject(s)
Internationality
11.
Nurs Rep ; 12(3): 520-527, 2022 Jul 13.
Article in English | MEDLINE | ID: mdl-35894040

ABSTRACT

Telehealth is increasingly taking place to support the transition of care and self-management of people living with cancer in outpatient oncology settings. Despite its recognised value, the scientific evidence points to disparities with regard to implementation of telehealth that might compromise the equity of access. Following the Joanna Briggs Institute (JBI) implementation approach, this project aims to promote the implementation of best practice recommendations for telehealth adoption in an outpatient oncology setting. Assisted by the Practical Application of Clinical Evidence System (PACES), the implementation process comprises three phases of (i) a baseline audit, (ii) feedback to the healthcare team and establishment of implementation strategies with the Getting Research into Practice (GRiP) tool, and (iii) a follow-up audit. The project is expected to allow the identification of barriers and facilitators for the implementation of telehealth in outpatient oncology and develop a strategy plan for its adoption, with the involvement of end-users and stakeholders. The successful adoption of telehealth according to the best available evidence will likely enhance equity of access to healthcare and quality of care at a distance.

12.
Prev Vet Med ; 206: 105698, 2022 Sep.
Article in English | MEDLINE | ID: mdl-35809462

ABSTRACT

Wild boar (Sus scrofa) is an exotic invasive species in Brazil and may be a reservoir for several pathogens, including those related to the porcine respiratory disease complex (PRDC), a critical infectious disease in pig production. The objective of this study was to investigate viral and bacterial pathogens related to PRDC in free-living wild boars from Brazil. Eighty animals were examined in search of genomes of porcine circovirus 2 (PCV2), Torque teno Sus virus 1a (TTSuV1a) and 1b (TTSuV1b), Influenza A virus (IAV), Actinobacillus pleuropneumoniae, Glaesserella parasuis, Pasteurella multocida, and Mycoplasma hyopneumoniae. The results demonstrated that 57.5% (46/80) of the animals had at least one detected pathogen, and 11.3% of them (9/80) were co-infected. TTSuV1a was the most prevalent genome, for which risk factors were associated with increased contact between wild boars and other animals. The other pathogens were detected at much lower frequencies or not detected (M. hyopneumoniae and IAV). An additional IAV serology search identified H1N1pdm09 antibodies in 35.5% (16/45) of the wild boars, bringing concern related to public health. In conclusion, wild boars are infected with pathogens that cause swine diseases, so their eventual contact with domestic pigs might risk animal production in Brazil.


Subject(s)
Circovirus , Mycoplasma hyopneumoniae , Swine Diseases , Animals , Antibodies, Viral , Brazil/epidemiology , Sus scrofa , Swine , Swine Diseases/microbiology
13.
Nurs Rep ; 12(2): 365-370, 2022 May 07.
Article in English | MEDLINE | ID: mdl-35645361

ABSTRACT

BACKGROUND: In palliative care, symptoms are multiple and combined, evolving and changing, with a multidimensional character and multifactorial causes, and a high prevalence, negatively influencing the patient's and family's quality of life. Nurses who provide palliative care need to recognize and respond effectively to their patients' symptoms. METHODS: A project will be applied to implement the best practice in monitoring and managing palliative care patients' symptoms. The Joanna Briggs Institute Practical Application of Clinical Evidence System (JBI PACES) and Getting Research into Practice (GRiP) audit and feedback tool will be used. The JBI PACES and GRiP framework for promoting evidence-based healthcare involves three phases of activity. First, a baseline audit. In a second phase, feedback will be given to the project team after the conclusion of the baseline audit report. Then, a third phase will be conducted as a follow-up audit. CONCLUSIONS: This project will improve the practice of the nursing team in monitoring and managing the symptoms of palliative care patients, positively influencing the quality of life of the patient and his family. The implementation and dissemination of this project could boost its replication in other centres.

14.
Zoonoses Public Health ; 69(6): 682-693, 2022 09.
Article in English | MEDLINE | ID: mdl-35569138

ABSTRACT

Bacterial resistance is a public and one health problem. Free-living birds can be reservoirs of multidrug-resistant bacteria and resistance genes. This study aimed to characterize the antimicrobial resistance of Escherichia coli isolated from free-living urban pigeons (Columba livia) in South Brazil. Ninety-two animals were sampled, and one isolate was obtained from each one. The isolates were characterized, and the antimicrobial resistance profile and beta-lactam and colistin resistance genes were investigated. The isolates were classified as phylogroups B1 (35%), B2 (33%), A (16%) and D (16%), and 14% of the strains had the eae virulence gene. All isolates were resistant to at least one antimicrobial, and 63% of them were multidrug-resistant. Geographical location where the pigeons were captured and presence of the eae gene were associated with multidrug resistance. blaVIM and mcr-1 genes were detected in one and two isolates, respectively. This is the first report of these genes in E. coli of pigeons. The blaVIM -positive isolate was classified as Shiga toxin-producing E. coli, and the isolates with mcr-1 were classified as Enterohaemorrhagic E. coli and Enteropathogenic E. coli, which raise additional concerns related to public health since these are zoonotic pathotypes. The results reveal that pigeons carry multidrug-resistant pathogenic E. coli, which may interest public health. Nonetheless, further studies on whether these animals are sources of contamination for humans must be performed to understand their role in spreading antimicrobial resistance.


Subject(s)
Anti-Infective Agents , Enteropathogenic Escherichia coli , Escherichia coli Infections , Escherichia coli Proteins , Animals , Anti-Bacterial Agents/pharmacology , Columbidae/microbiology , Escherichia coli Infections/epidemiology , Escherichia coli Infections/microbiology , Escherichia coli Infections/veterinary , Escherichia coli Proteins/genetics , Humans , Microbial Sensitivity Tests/veterinary
15.
Anat Histol Embryol ; 51(3): 390-399, 2022 May.
Article in English | MEDLINE | ID: mdl-35218238

ABSTRACT

Eira barbara, popularly known as irara, is a medium-sized carnivore member of the Mustelidae family. Despite its important role in the ecosystems in which its lives, data on the internal morphology of E. barbara remains scarce. Therefore, this study aimed to provide knowledge regarding the anatomy of the male reproductive system of this species to improve understanding of its reproduction to inform its conservation. We studied seven specimens who had died after being run over. The specimens were dissected for the evaluation of the reproductive system, which comprised a globular scrotum; a small pendulum covered with light-coloured hair; a pair of testicles of firm consistency and ellipsoid shape and suspended in the scrotum by the spermatic cord; a paired duct system; an ampoule of the deferens duct and prostate constituting the set of attached glands; a urethra divided into pelvic and penile portions; a penis with a baculum having a novel "C" shaped apex; and a prepuce. Microscopically, the testicular parenchyma consisted of seminiferous tubules separated by intertubular spaces formed by loose connective tissue, fibrocytes, Leydig cells, and blood and lymph vessels. The epididymis was surrounded by a capsule of dense connective tissue and extended to form septa. The baculum was microscopically classified as a compact bone containing several bony lamellae with osteocytes and osteoblasts. The macro and microscopic findings were generally similar to those of domestic carnivores, with some notable differences.


Subject(s)
Ecosystem , Mustelidae , Animals , Epididymis/anatomy & histology , Genitalia, Male/anatomy & histology , Male , Penis/anatomy & histology
16.
An Acad Bras Cienc ; 93(suppl 4): e20201682, 2021.
Article in English | MEDLINE | ID: mdl-34878047

ABSTRACT

In canine visceral leishmaniasis, coinfections can aggravate the disease. Our aim was to investigate Brucella canis in dogs infected with Leishmania infantum. One hundred and six L. infantum-seropositive dogs were submitted to serology for B. canis, PCR for B. canis and L. infantum, and histopathological analysis of the genital tract. Anti-B. canis antibodies were detected in seven dogs whose clinical signs, L. infantum load and histological alterations were similar to those of seronegative animals. The circulation of anti-B. canis antibodies was low but demonstrates the exposure of dogs to this bacterium in a visceral leishmaniasis-endemic area.


Subject(s)
Dog Diseases , Leishmania infantum , Leishmaniasis, Visceral , Animals , Dogs , Genitalia , Leishmania infantum/genetics , Leishmaniasis, Visceral/veterinary , Polymerase Chain Reaction
17.
J Equine Vet Sci ; 107: 103779, 2021 12.
Article in English | MEDLINE | ID: mdl-34802621

ABSTRACT

Antimicrobial resistance (AMR) is a global concern that must be addressed from a one health perspective. Horses are companion animals and their contact with humans facilitates exchange of resistant bacteria. This study aimed to evaluate AMR of coagulase-positive Staphylococcus (CoPS), including Staphylococcus aureus, isolated from healthy Crioulo horses. Swab samples from nostrils (n = 214) and skin (n = 107) of 107 horses from Porto Alegre, South Brazil, were used for CoPS isolation. The isolates were evaluated for AMR and a multivariate logistic regression was applied to identify the risk factors associated to this outcome, using information on horses' management and installations where they were maintained. A total of 143 CoPS were isolated from 79 horses (73.8%), of which 8 (5.6%) were S. aureus. The isolates showed resistance to seven of 10 tested antimicrobials and 38.5% (55/143) of them were resistant to at least one antimicrobial. One isolate (0.7%; 1/143) was classified as multidrug-resistant. Regarding S. aureus, 62.5 % (5/8) showed AMR, but none were methicillin-resistant. The risk factors associated with CoPS' antimicrobial resistance were lower frequency of bed changing (OR = 6.40; P = .001) and nonaccumulation of bed materials (OR = 3.47; P = .002). The results point that healthy horses have antimicrobial-resistant CoPS and S. aureus in their microbiota, which may be of concern for animal and human health. Moreover, bed management was associated with AMR, which can serve as a guide for best practices to be adopted to avoid the occurrence of resistant bacteria in these animals.


Subject(s)
Anti-Infective Agents , Coagulase , Animals , Anti-Bacterial Agents/pharmacology , Drug Resistance, Bacterial , Horses , Risk Factors , Staphylococcus , Staphylococcus aureus
18.
Rev Esc Enferm USP ; 55: e20210180, 2021.
Article in English, Portuguese | MEDLINE | ID: mdl-34807227

ABSTRACT

OBJECTIVE: To report the experience of the Portugal Centre For Evidence Based Practice (PCEBP): a JBI Centre of Excellence in the training of health professionals, researchers, and professors in the Comprehensive Systematic Review Training Program, a course on Evidence Synthesis, specifically on Systematic Literature Reviews. METHOD: This article aims to report the experience of the Portugal Centre For Evidence Based Practice: a JBI Centre of Excellence in the implementation of the Comprehensive Systematic Review Training Program that trains health professionals, researchers, and teachers to develop Systematic Reviews, according to the JBI approach. RESULTS: By the end of 2020, 11 editions of the course had been developed with 136 participants from different educational and health institutions, from different countries. As a result of the training of these participants, 13 systematic reviews were published in JBI Evidence Synthesis and 10 reviews were published in other journals. CONCLUSION: The reported results and the students' satisfaction evaluation allow us to emphasize the relevance of the course for health professionals training on evidence synthesis.


Subject(s)
Evidence-Based Practice , Health Personnel , Humans , Portugal , Systematic Reviews as Topic
19.
Comp Immunol Microbiol Infect Dis ; 79: 101716, 2021 Dec.
Article in English | MEDLINE | ID: mdl-34715476

ABSTRACT

The expansion of urbanization on natural areas is increasing contact between human populations with wild animals. Wild carnivores can act as sentinel hosts or environmental health indicators. Thus, the aim of this work was to investigate the exposure of two major species of wild canids from Southern Brazil to selected pathogens. For that, we live-trapped free-ranging Cerdocyon thous and Lycalopex gymnocercus in five localities and determined the frequency of animals with antibodies against Toxoplasma gondii, Trypanosoma cruzi, Leishmania infantum, Neospora caninum, and Leptospira spp. Among the canids sampled, 23% (12/52) (95%CI: 13-36%) had antibodies against T. gondii, with titers ranging from 64 to 512. For T. cruzi, 28% (15/52) (95%CI: 18-42%) of sampled canids were seropositive, with titers ranging from 8 to 64. Concerning the protozoan pathogen N. caninum, a total of 5% (3/52) (95%CI: 2-15%) of wild canids had antibodies against it. None of the sampled canids showed the presence of antibodies against L. infantum. On the other hand, 44% (23/52) (95%CI: 31-57%) of the wild canids showed antibodies against Leptospira spp. The set of results presented here, show that free-ranging neotropical wild canids are exposed and have antibodies against to T. gondii, T. cruzi, Leptospira spp., and to a lesser degree to N. caninum. We found no evidence of L. infantum circulation among the studied populations. These results highlight some of the major pathogens which may represent risks for populations of these wild canids. DATA AVAILABILITY STATEMENT: The data that support the findings of this study are available from the corresponding author upon reasonable request.


Subject(s)
Canidae , Coccidiosis , Neospora , Toxoplasma , Toxoplasmosis, Animal , Animals , Antibodies, Protozoan , Brazil/epidemiology , Coccidiosis/veterinary , Seroepidemiologic Studies , Toxoplasmosis, Animal/epidemiology
20.
Hist Cienc Saude Manguinhos ; 28(4): 1281-1286, 2021.
Article in Portuguese | MEDLINE | ID: mdl-34346999

ABSTRACT

Scientific policy in Brazil has neglected the human and social sciences, especially in terms of investing in research. With the arrival of covid-19, efforts have focused on minimizing the impact of this health crisis on the country's social and economic life. In the scientific arena, this involved research financing and professional qualification grants that prioritized technological areas while restricting opportunities for researchers from the social, human, and artistic sciences. Here we maintain that Brazil's scientific policy is out of step with international scientific development programs, and has a limited view of what science is, as well as its role in the nation's development.


A política científica no Brasil tem negligenciado o papel das áreas humanas e sociais, sobretudo quanto a investimento em pesquisa. Com a pandemia de covid-19, as atenções se voltaram para minimizar o impacto da crise sanitária na vida social e econômica do país. No universo científico, isso se efetivou por meio de editais de fomento a pesquisa e bolsas de qualificação profissional que estabeleceram como prioritárias as áreas tecnológicas, restringindo as oportunidades de pesquisadores de ciências sociais, humanas e artísticas. Argumenta-se aqui que a política científica desenvolvida no Brasil está em descompasso com programas internacionais de desenvolvimento científico, demonstrando uma visão limitada do que é ciência, bem como de seu papel no desenvolvimento de uma nação.


Subject(s)
COVID-19 , Brazil , Humans , Policy , SARS-CoV-2 , Social Sciences
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