ABSTRACT
The elaboration of distinct cell types during development is dependent on a small number of inductive molecules. Among these inducers is Sonic hedgehog (Shh), which, in combination with other factors, patterns the dorsoventral (DV) axis of the nervous system. The response of a cell is dependent in part on its complement of cyclic nucleotides. cAMP antagonizes Shh signaling, and we examined the influence of cGMP on the Shh response. Cells in chick neural plate explants respond to Shh by differentiating into ventral neural-cell types. Exposure of intermediate-zone explants to cGMP analogs enhanced their response to Shh in a dose-dependent manner. The Shh response was also enhanced in dorsal-zone explants exposed to chick natriuretic peptide (chNP), which stimulates cGMP production by membrane-bound guanylate cyclase (mGC). Addition of chNP to intermediate-zone explants did not enhance the Shh response, consistent with a reported lack of mGC in this region of the neural tube. Finally, the presence of a nitric oxide (NO)-sensitive guanylate cyclase (GC) was established by demonstrating cGMP immunoreactivity in neural tissue following NO stimulation of whole chick embryos. Intracellular levels of cGMP and cAMP may thus provide a mechanism through which other factors modulate the Shh response during neural development.
Subject(s)
Cyclic GMP/metabolism , Neural Crest/cytology , Neural Crest/embryology , Trans-Activators/metabolism , Animals , Cell Differentiation , Cell Lineage , Chick Embryo , Cyclic AMP/metabolism , Dose-Response Relationship, Drug , Gene Expression Regulation, Developmental , Guanylate Cyclase/metabolism , Hedgehog Proteins , Humans , Ligands , Neural Crest/metabolism , Neurons/metabolism , Nitric Oxide/metabolism , Phosphorylation , Protein Binding , Spinal Cord/embryologyABSTRACT
Holoprosencephaly, or an undivided forebrain, is a complex brain malformation associated with Sonic hedgehog (Shh) mutations. Other causes of holoprosencephaly have converged upon the Shh signaling pathway: genetic and pharmacologic impairment of cholesterol synthesis, and the action of the steroidal alkaloid cyclopamine. This review focuses on recent studies aimed at determining how Shh signaling is affected by these causes of holoprosencephaly, whether they involve a common mechanism and the role played by cholesterol. Cholesterol is potentially important for both biogenesis of Shh and in signal transduction in Shh-responsive cells. Teratogens that induce holoprosencephaly appear to affect Shh signal transduction rather than Shh biogenesis. Analysis of these agents and other compounds that affect various aspects of cellular cholesterol distribution indicates that the role of cholesterol in Shh signal transduction is novel and complicated. The similarity of the Shh receptor, Patched (Ptc), to the Niemann-Pick Cl protein, which is involved in the vesicular trafficking of cholesterol, provides insight into the role of cholesterol and the action of compounds like cyclopamine.
Subject(s)
Carrier Proteins , Cholesterol/physiology , Holoprosencephaly/etiology , Membrane Glycoproteins , Proteins/physiology , Trans-Activators , Animals , Biological Transport, Active , Hedgehog Proteins , Holoprosencephaly/genetics , Intracellular Signaling Peptides and Proteins , Membrane Proteins/physiology , Models, Biological , Mutation , Niemann-Pick C1 Protein , Patched Receptors , Proteins/genetics , Receptors, Cell Surface , Signal Transduction/drug effects , Teratogens/toxicity , Veratrum Alkaloids/pharmacology , trans-1,4-Bis(2-chlorobenzaminomethyl)cyclohexane Dihydrochloride/pharmacologyABSTRACT
Patched (Ptc) is the ligand-binding component of the Hedgehog (Hh) receptor complex. In the Drosophila embryo, Ptc and Hh colocalize in vesicular punctate structures. However, receptor-mediated endocytosis of Hh proteins has not been demonstrated. By using chick neural plate explants, we show that Sonic hedgehog (Shh)-responsive neural precursor cells internalize recombinant and endogenous Shh and provide direct evidence for a gradient of endogenous Shh in the ventral neural tube. Shh internalization is blocked by a monoclonal antibody whose epitope overlaps the Ptc-binding site of Shh. These findings suggest that Shh internalization is mediated by Ptc-1 and may be linked to signaling. Concordantly, transfection of mammalian cell lines with a Ptc-1 cDNA confers the ability to internalize multiple forms of Shh, including transmembrane-anchored Shh, by a dynamin-dependent process.
Subject(s)
Drosophila Proteins , Endocytosis , Insect Proteins/metabolism , Membrane Proteins/metabolism , Proteins/metabolism , Receptors, Cell Surface/metabolism , Trans-Activators , Animals , Cell Line , Dynamins , GTP Phosphohydrolases/metabolism , Hedgehog Proteins , Humans , Nervous System/cytology , Nervous System/embryology , Nervous System/metabolism , Signal TransductionABSTRACT
Cyclopamine is a teratogenic steroidal alkaloid that causes cyclopia by blocking Sonic hedgehog (Shh) signal transduction. We have tested whether this activity of cyclopamine is related to disruption of cellular cholesterol transport and putative secondary effects on the Shh receptor, Patched (Ptc). First, we report that the potent antagonism of Shh signaling by cyclopamine is not a general property of steroidal alkaloids with similar structure. The structural features of steroidal alkaloids previously associated with the induction of holoprosencephaly in whole animals are also associated with inhibition of Shh signaling in vitro. Second, by comparing the effects of cyclopamine on Shh signaling with those of compounds known to block cholesterol transport, we show that the action of cyclopamine cannot be explained by inhibition of intracellular cholesterol transport. However, compounds that block cholesterol transport by affecting the vesicular trafficking of the Niemann-Pick C1 protein (NPC1), which is structurally similar to Ptc, are weak Shh antagonists. Rather than supporting a direct link between cholesterol homeostasis and Shh signaling, our findings suggest that the functions of both NPC1 and Ptc involve a common vesicular transport pathway. Consistent with this model, we find that Ptc and NPC1 colocalize extensively in a vesicular compartment in cotransfected cells.
Subject(s)
Carrier Proteins , Cholesterol/metabolism , Membrane Glycoproteins , Proteins/metabolism , Signal Transduction/drug effects , Trans-Activators , Veratrum Alkaloids/pharmacology , Androstenes/pharmacology , Animals , Biological Transport/drug effects , COS Cells , Chick Embryo , Hedgehog Proteins , Intracellular Signaling Peptides and Proteins , Niemann-Pick C1 Protein , Oncogene Proteins/metabolism , Progesterone/pharmacology , Recombinant Proteins/metabolism , TransfectionABSTRACT
Recent genetic studies have shown that the signalling factor Wnt3a is required for formation of the hippocampus; the developmental consequences of Wnt signalling in the hippocampus are mediated by multiple HMG-box transcription factors, with LEF-1 being required just for formation of the dentate gyrus.
Subject(s)
DNA-Binding Proteins/genetics , High Mobility Group Proteins/genetics , Hippocampus/embryology , Proteins/genetics , Transcription Factors/genetics , Animals , Embryonic Induction , Lymphoid Enhancer-Binding Factor 1 , Mice , Mice, Mutant Strains , Wnt Proteins , Wnt3 Protein , Wnt3A ProteinABSTRACT
Numerous naturally-occurring and synthetic compounds that were discovered initially because of their toxic properties, were later shown to possess biological activities beneficial to humans that enabled them to serve as templates for the development of useful medicinal agents. A prominent example is thalidomide, a synthetic drug that gained notoriety originally due to its catastrophic teratogenicity in humans. The discovery of thalidomide's efficacy in treating several diseases has resulted in the recrudescence of the drug to society's usage. A current example of this phenomenon is the plant teratogen cyclopamine (11-deoxojervine), whose deleterious terata-inducing effects were restricted to grazing animals, but whose recently discovered inhibition of Sonic hedgehog signal transduction has provided both the potential to increase our understanding of organogenesis and to serve as a lead compound in drug development.
Subject(s)
Proteins/metabolism , Teratogens/pharmacology , Thalidomide/pharmacology , Trans-Activators , Veratrum Alkaloids/pharmacology , Abnormalities, Drug-Induced/etiology , Animals , Bioethics , Hedgehog Proteins , Humans , Hypnotics and Sedatives/chemistry , Hypnotics and Sedatives/pharmacology , Proteins/drug effects , Proteins/genetics , Signal Transduction , Teratogens/chemistry , Thalidomide/chemistry , Veratrum Alkaloids/chemistryABSTRACT
We used a degenerate polymerase chain reaction (PCR) strategy to search for Wnt RNA in developing ocular tissues. We isolated a Macaca monkey Wnt-13 PCR fragment, orthologous to the human and murine Wnt-13 and Xenopus Wnt-2b, and a chick Wnt13 cDNA. Wnt-13 is a member of the Wnt-1 class of transforming Wnt molecules. In situ RNA hybridization revealed a dynamic Wnt-13 expression pattern in numerous developing tissues. Within the eye, Wnt-13 is expressed in the proliferative epithelium of the lens and both pigmented and non-pigmented layers of the ciliary margin. In vitro BrdU incorporation studies coupled with in situ hybridization showed that cWnt-13 expression domains in the lens were coincident with cell division. In addition to the eye, cWnt-13 was expressed in head ectoderm, prospective forelimb mesenchyme, lung bud, pharyngeal arches, the brain, as well as the otic vesicle. Our data are consistent with previous observations linking transforming Wnts with cell division and implicate a cascade of events involving cWnt-13 first in dorsoventral patterning and later in cell proliferation regulation associated with lens development. Dev Dyn 1999;215:215-224.
Subject(s)
Chickens/genetics , Embryonic Induction/physiology , Eye Proteins/biosynthesis , Gene Expression Regulation, Developmental , Glycoproteins/biosynthesis , Intercellular Signaling Peptides and Proteins , Amino Acid Sequence , Animals , Cell Division , Chick Embryo , Eye Proteins/genetics , Glycoproteins/genetics , Humans , In Situ Hybridization , Lens, Crystalline/embryology , Macaca nemestrina , Mice , Molecular Sequence Data , Morphogenesis/genetics , Organ Specificity , Pigment Epithelium of Eye/metabolism , Polymerase Chain Reaction , RNA, Messenger/biosynthesis , Sequence Alignment , Sequence Homology, Amino Acid , Wnt Proteins , Xenopus laevisABSTRACT
Characterization of the molecular pathways controlling differentiation and proliferation in mammalian hair follicles is central to our understanding of the regulation of normal hair growth, the basis of hereditary hair loss diseases, and the origin of follicle-based tumors. We demonstrate that the proto-oncogene Wnt3, which encodes a secreted paracrine signaling molecule, is expressed in developing and mature hair follicles and that its overexpression in transgenic mouse skin causes a short-hair phenotype due to altered differentiation of hair shaft precursor cells, and cyclical balding resulting from hair shaft structural defects and associated with an abnormal profile of protein expression in the hair shaft. A putative effector molecule for WNT3 signaling, the cytoplasmic protein Dishevelled 2 (DVL2), is normally present at high levels in a subset of cells in the outer root sheath and in precursor cells of the hair shaft cortex and cuticle which lie immediately adjacent to Wnt3-expressing cells. Overexpression of Dvl2 in the outer root sheath mimics the short-hair phenotype produced by overexpression of Wnt3, supporting the hypothesis that Wnt3 and Dvl2 have the potential to act in the same pathway in the regulation of hair growth. These experiments demonstrate a previously unrecognized role for WNT signaling in the control of hair growth and structure, as well as presenting the first example of a mammalian phenotype resulting from overexpression of a Dvl gene and providing an accessible in vivo system for analysis of mammalian WNT signaling pathways.
Subject(s)
Gene Expression Regulation, Developmental/genetics , Hair/growth & development , Proteins/genetics , Proto-Oncogene Proteins/genetics , Signal Transduction/genetics , Adaptor Proteins, Signal Transducing , Animals , Cell Differentiation/genetics , Dishevelled Proteins , Electrophoresis, Gel, Two-Dimensional , Epidermis/embryology , Fluorescent Antibody Technique , Hair/cytology , Hair/ultrastructure , Hair Follicle/cytology , Hair Follicle/growth & development , Histocytochemistry , In Situ Hybridization , Mice , Mice, Transgenic , Microscopy, Electron , Phenotype , Phosphoproteins , RNA, Messenger/metabolism , Transgenes/genetics , Wnt Proteins , Wnt3 ProteinABSTRACT
The steroidal alkaloid cyclopamine produces cyclopia and holoprosencephaly when administered to gastrulation-stage amniote embryos. Cyclopamine-induced malformations in chick embryos are associated with interruption of Sonic hedgehog (Shh)-mediated dorsoventral patterning of the neural tube and somites. Cell types normally induced in the ventral neural tube by Shh are either absent or appear aberrantly at the ventral midline after cyclopamine treatment, while dorsal cell types normally repressed by Shh appear ventrally. Somites in cyclopamine-treated embryos show Pax7 expression throughout, indicating failure of sclerotome induction. Cyclopamine at concentrations of 20-100 nM blocks the response of neural plate explants to recombinant Shh-N in a dose-dependent manner. Similar concentrations have no effect on the post-translational modification of Shh by cholesterol in transfected COS-1 cells. Comparison of the effects of cyclopamine to those of the holoprosencephaly-inducing cholesterol synthesis inhibitor AY-9944 shows that cyclopamine does not induce malformations by interfering with cholesterol metabolism. Although AY-9944 does not interrupt Shh signaling in ovo, it blocks the response to Shh-N in explants cultured without an exogenous cholesterol source. As predicted by current models of the regulation of cholesterol metabolism, the response to Shh-N in AY-9944-treated explants is restored by providing exogenous cholesterol. However, exogenous cholesterol does not restore Shh signaling in cyclopamine-treated explants. These findings suggest that cyclopamine-induced teratogenesis is due to a more direct antagonism of Shh signal transduction.
Subject(s)
Antihypertensive Agents/pharmacology , Homeodomain Proteins , Proteins/antagonists & inhibitors , Signal Transduction/drug effects , Teratogens/pharmacology , Trans-Activators , Veratrum Alkaloids/pharmacology , Animals , Anticholesteremic Agents/pharmacology , Body Patterning , COS Cells , Central Nervous System/embryology , Chick Embryo , Cholesterol/metabolism , Hedgehog Proteins , Muscle Proteins/biosynthesis , Nerve Tissue Proteins/biosynthesis , PAX7 Transcription Factor , Proteins/genetics , Transfection , trans-1,4-Bis(2-chlorobenzaminomethyl)cyclohexane Dihydrochloride/pharmacologyABSTRACT
The hedgehog gene family encodes secreted proteins important in many developmental patterning events in both vertebrates and invertebrates. In the Drosophila eye disk, hedgehog controls the progression of photoreceptor differentiation in the morphogenetic furrow. To investigate whether hedgehog proteins are also involved in the development of the vertebrate retina at stages of photoreceptor differentiation, we analyzed expression of the three known vertebrate hedgehog genes. We found that Sonic hedgehog and Desert hedgehog are expressed in the developing retina, albeit at very low levels, whereas Indian hedgehog (Ihh) is expressed in the developing and mature retinal pigmented epithelium, beginning at embryonic day 13. To determine whether hedgehog proteins have activities on developing retinal cells, we used an in vitro system in which much of retinal histogenesis is recapitulated. N-terminal recombinant Sonic Hedgehog protein (SHH-N) was added to rat retinal cultures for 3-12 d, and the numbers of retinal cells of various phenotypes were analyzed by immunohistochemistry. We found that SHH-N caused a transient increase in the number of retinal progenitor cells, and a 2- to 10-fold increase in the number of photoreceptors differentiating in the cultures when analyzed with three different photoreceptor-specific antigens. In contrast, the numbers of retinal ganglion cells and amacrine cells were similar to those in control cultures. These results show that Hedgehog proteins can regulate mitogenesis and photoreceptor differentiation in the vertebrate retina, and Ihh is a candidate factor from the pigmented epithelium to promote retinal progenitor proliferation and photoreceptor differentiation.
Subject(s)
Drosophila Proteins , Proteins/pharmacology , Retinal Rod Photoreceptor Cells/cytology , Trans-Activators , Animals , Animals, Newborn , Cell Differentiation/drug effects , Cells, Cultured , Embryonic Induction , Eye/embryology , Female , Fetus/cytology , Hedgehog Proteins , Insect Proteins/genetics , Mammals , Pregnancy , Proteins/genetics , RNA, Messenger/analysis , Rats , Rats, Sprague-Dawley , Recombinant Proteins/pharmacology , Retinal Rod Photoreceptor Cells/chemistry , Retinal Rod Photoreceptor Cells/physiology , Stem Cells/cytology , Stem Cells/drug effectsABSTRACT
Antibodies that block Sonic Hedgehog (SHH) signaling have been used to show that SHH activity is required for the induction of floor plate differentiation by the notochord and independently for the induction of motor neurons by both the notochord and midline neural cells. Motor neuron generation depends on two critical periods of SHH signaling: an early period during which naive neural plate cells are converted into ventralized progenitors and a late period that extends well into S phase of the final progenitor cell division, during which SHH drives the differentiation of ventralized progenitors into motor neurons. The ambient SHH concentration during the late period determines whether ventralized progenitors differentiate into motor neurons or interneurons, thus defining the pattern of neuronal cell types generated in the neural tube.
Subject(s)
Central Nervous System/embryology , Embryonic Induction , Motor Neurons , Notochord/metabolism , Proteins/metabolism , Trans-Activators , Animals , Body Patterning , Cell Differentiation , Cell Division , Central Nervous System/cytology , Chick Embryo , Hedgehog Proteins , Interneurons , Models, Biological , Stem Cells , Time FactorsABSTRACT
Near the floor plate of the embryonic neural tube there is a group of neuroepithelial precursor cells that are specialized for production of the oligodendrocyte lineage. We performed experiments to test whether specification of these neuroepithelial oligodendrocyte precursors, like other ventral neural cell types, depends on signals from the notochord and/or floor plate. We analyzed heterozygous Danforth's short tail (Sd/+) mutant mice, which lack a notochord and floor plate in caudal regions of the neural tube, and found that oligodendrocyte precursors did not appear at the ventricular surface where there was no floor plate. Moreover, oligodendrocytes did not develop in explant cultures of Sd/+ spinal cord in the absence of a floor plate. When a second notochord was grafted into an ectopic position dorsolateral to the endogenous notochord of a chicken embryo, an additional floor plate was induced along with an ectopic focus of oligodendrocyte precursors at the ventricular surface. Oligodendrocytes developed in explants of intermediate neural tube only when they were cocultured with fragments of notochord or in the presence of purified Sonic hedgehog (Shh) protein. Thus, signals from the notochord/floor plate, possibly involving Shh, are necessary and sufficient to induce the development of ventrally derived oligodendroglia. These signals appear to act by specifying the future fate(s) of neuroepithelial cells at the ventricular surface rather than by influencing the proliferation or differentiation of prespecified progenitor cells in the parenchyma of the cord.
Subject(s)
Embryonic Induction/genetics , Nervous System/embryology , Notochord/embryology , Oligodendroglia/physiology , Proteins/genetics , Stem Cells/physiology , Trans-Activators , Animals , Cell Lineage/physiology , Cell Movement , Cells, Cultured , Chick Embryo , Epithelial Cells , Epithelium/embryology , Female , Fetal Tissue Transplantation , Hedgehog Proteins , In Situ Hybridization , Mice , Mice, Mutant Strains , Microscopy, Confocal , Nervous System/cytology , Notochord/transplantation , Oligodendroglia/cytology , Pregnancy , Spinal Cord/embryologyABSTRACT
A central issue in embryonic development is the resolution of how groups of equivalent cells are transformed into orderly and patterned arrays of distinct cell types. Recent studies suggest the involvement of the Hedgehog, Wnt and bone morphogenetic protein families in the patterning of different tissue types in vertebrate embryos. The integrated actions of members of these three families of signaling proteins appear to have been recruited in the patterning of neural tissue in addition to several different tissues. Over the past year, a clearer picture of the diverse roles of these signaling proteins in embryonic development has begun to emerge.
Subject(s)
Neurons/physiology , Signal Transduction/physiology , Vertebrates/growth & development , Animals , Hedgehog Proteins/metabolism , Models, NeurologicalABSTRACT
The cellular interactions that control the differentiation of dorsal cell types from neural progenitors have been examined in neural plate explants. Certain genes that are expressed in the dorsal neural tube are initially expressed uniformly within the neural plate and appear to achieve their dorsal restriction through a Sonic hedgehog (SHH)-mediated repressive signal from the notochord. The acquisition of definitive dorsal cell fates, however, requires a contact-dependent signal from the epidermal ectoderm. BMP4 and BMP7 are expressed in the epidermal ectoderm, and both proteins mimic its inductive activity. BMP4 and a related gene, DSL1, are subsequently expressed by cells in the dorsal neural tube. The differentiation of dorsal cell types, therefore, appears to be initiated at the neural plate stage and to involve the opponent activities of a BMP-mediated dorsalizing signal from the epidermal ectoderm and a SHH-mediated ventralizing signal from the notochord.
Subject(s)
Ectoderm/cytology , Embryonic Induction/physiology , Neural Crest/cytology , Proteins/physiology , Transforming Growth Factor beta/physiology , Animals , Biomarkers , Bone Morphogenetic Proteins , Cell Communication/physiology , Cell Differentiation/physiology , Cell Line/physiology , Chick Embryo , Epidermal Cells , Gene Expression/physiology , Neural Crest/embryologyABSTRACT
BACKGROUND: The differentiation of floor plate cells and motor neurons in the vertebrate neural tube appears to be induced by signals from the notochord. The secreted protein encoded by the Sonic hedgehog (Shh) gene is expressed by axial midline cells and can induce floor plate cells in vivo and in vitro. Motor neurons can also be induced in vitro by cells that synthesize Sonic hedgehog protein (Shh). It remains unclear, however, if the motor-neuron-inducing activity of Shh depends on the synthesis of a distinct signaling molecule by floor plate cells. To resolve this issue, we have developed an in vitro assay which uncouples the notochord-mediated induction of motor neurons from floor plate differentiation, and have used this assay to examine whether Shh induces motor neurons in the absence of floor plate differentiation. RESULTS: Floor plate cells and motor neurons were induced in neural plate explants grown in contact with the notochord, but only motor neurons were induced when explants were separated from the notochord. COS cells transfected with Shh induced both floor plate cells and motor neurons when grown in contact with neural plate explants, whereas only motor neurons were induced when the explants were grown at a distance from Shh-transfected COS cells. Direct transfection of neural plate cells with an Shh-expression construct induced both floor plate cells and motor neurons, with motor neuron differentiation occurring prior to, or coincidentally with, floor plate differentiation. The induction of motor neurons appears, therefore, not to depend on floor plate differentiation. CONCLUSIONS: The induction of motor neurons by Shh does not depend on distinct floor-plate-derived signaling molecules. Shh can, therefore, initiate the differentiation of two cell types that are generated in the ventral region of the neural tube. These results show that the early development of motor neurons involves the inductive action of Shh, whereas the survival of motor neurons at later stages of embryonic development requires neurotrophic factors.
Subject(s)
Central Nervous System/embryology , Embryonic Induction/physiology , Motor Neurons/physiology , Proteins/physiology , Trans-Activators , Animals , Apoptosis , Base Sequence , Cell Differentiation , Cells, Cultured , Central Nervous System/physiology , Chlorocebus aethiops , Hedgehog Proteins , Molecular Sequence Data , Notochord/physiology , Signal Transduction/physiology , TransfectionABSTRACT
The differentiation of floor plate cells and motor neurons can be induced by Sonic hedgehog (SHH), a secreted signaling protein that undergoes autoproteolytic cleavage to generate amino- and carboxy-terminal products. We have found that both floor plate cells and motor neurons are induced by the amino-terminal cleavage product of SHH (SHH-N). The threshold concentration of SHH-N required for motor neuron induction is about 5-fold lower than that required for floor plate induction. Higher concentrations of SHH-N can induce floor plate cells at the expense of motor neuron differentiation. Our results suggest that the induction of floor plate cells and motor neurons by the notochord in vivo is mediated by exposure of neural plate cells to different concentrations of the amino-terminal product of SHH autoproteolytic cleavage.
Subject(s)
Embryonic Induction , Motor Neurons/physiology , Nervous System/embryology , Peptide Fragments/physiology , Proteins/physiology , Trans-Activators , Animals , Cell Differentiation , Cells, Cultured , Chick Embryo , Dose-Response Relationship, Drug , Hedgehog Proteins , Immunohistochemistry , In Vitro Techniques , Mice , Models, Biological , Nervous System/cytology , Notochord/embryology , Peptide Fragments/metabolism , Proteins/immunology , Proteins/metabolism , Rats , Recombinant Proteins/metabolism , Tissue DistributionABSTRACT
Intercellular signaling molecules of the vertebrate hedgehog family and transcription factors of the winged-helix family have been implicated in floor plate development. We have examined the consequences of misexpressing the vertebrate hedgehog gene vhh-1 (sonic hedgehog, shh) and the winged-helix gene HNF-3 beta in the neural plate and neural tube of frog embryos. Misexpression of either of these genes induces floor plate differentiation at ectopic locations. However, ectopic floor plate induction in response to both vhh-1 and HNF-3 beta was temporally and spatially restricted. At neural plate stages, ectopic floor plate differentiation was not detected. After neural tube closure, ectopic floor plate differentiation was detected but was restricted predominantly to the dorsal region of the neural tube. The ability of winged-helix and vertebrate hedgehog genes to induce floor plate differentiation in vivo may, therefore, be constrained by additional signals that specify the time and position of floor plate differentiation.
Subject(s)
Anura/embryology , Anura/genetics , DNA-Binding Proteins/genetics , Embryonic Induction/genetics , Nuclear Proteins/genetics , Proteins/genetics , Trans-Activators , Transcription Factors/genetics , Animals , Brain/embryology , Cell Differentiation/genetics , Female , Gastrula/physiology , Gene Expression/physiology , Hedgehog Proteins , Hepatocyte Nuclear Factor 3-beta , Immunohistochemistry , In Situ Hybridization , Microinjections , Neurons/physiology , Notochord/physiology , Plasmids , Spinal Cord/embryology , Time FactorsABSTRACT
The differentiation of distinct cell types in the ventral neural tube depends on local inductive signals from the notochord. We have isolated a vertebrate homolog of the Drosophila segment polarity gene hedgehog (hh) from zebrafish and rat, termed vhh-1. vhh-1 is expressed in the node, notochord, floor plate, and posterior limb bud mesenchyme. Each of these cell groups has floor plate inducing activity, suggesting that the vhh-1 gene may encode a floor plate-inducing molecule. Widespread expression of rat vhh-1 in frog embryos leads to ectopic floor plate differentiation in the neural tube. In vitro tests for the signaling functions of vhh-1 demonstrate that COS cells expressing the rat vhh-1 gene induce floor plate and motor neuron differentiation in neural plate explants. vhh-1 may, therefore, contribute to the floor plate and motor neuron inducing activities of the notochord.
Subject(s)
Drosophila Proteins , Embryonic Induction , Nervous System/embryology , Proteins/genetics , Trans-Activators , Amino Acid Sequence , Animals , Base Sequence , Cell Differentiation , Chick Embryo , DNA Primers/chemistry , Extremities/embryology , Gene Expression , Hedgehog Proteins , In Situ Hybridization , Molecular Sequence Data , Motor Neurons/cytology , Proteins/chemistry , RNA, Messenger/genetics , Rats , Sequence Alignment , Sequence Homology, Amino Acid , Solubility , Xenopus laevis , Zebrafish/genetics , Zebrafish ProteinsABSTRACT
In mouse mammary tumors, the Wnt-3 gene can be activated by proviral insertion. Here we report on the isolation of a human homolog, WNT3. A genomic clone was isolated by use of mouse Wnt-3 sequences as a probe, after which cDNA containing most of the protein-encoding domain of the human gene was obtained by PCR. Comparison between the deduced mouse and human WNT-3 protein sequences showed four changes in 333 amino acids. WNT3 is located on chromosome 17q21. The gene was not found to be amplified or rearranged in a collection of human breast tumors.
Subject(s)
Chromosomes, Human, Pair 17 , Proto-Oncogenes , Amino Acid Sequence , Animals , Base Sequence , Breast Neoplasms/genetics , Chromosome Mapping , Cloning, Molecular , Conserved Sequence , DNA, Complementary/genetics , Female , Humans , Hybrid Cells , Mice , Molecular Sequence Data , Proteins/genetics , Sequence Homology, Amino Acid , Species Specificity , Wnt Proteins , Wnt3 ProteinABSTRACT
Mammary tumors in the GR strain are caused by a dominant locus containing an endogenous mouse mammary tumor provirus. Expression of this locus results in high virus titers, inducing tumors that progress from a hormone-dependent to a hormone-independent tumor state. We previously studied the activation of the Wnt-1 and int-2 oncogenes in several series of transplanted GR tumors and found that hormone-dependent early passages are generally oligoclonal for proviral integration at these genes. We have now re-examined several such tumor series for activation of other Wnt genes. In one series, the transition to hormone-independent growth was marked by the loss of the oligoclonal genotype and outgrowth of a hormone-independent cell population, clonal for the activation of Wnt-3. We show two examples of series of transplanted tumors that in later hormone-independent passages contain an amplified and overexpressed Wnt-2 gene, a novel mode of activation of these genes.