Inflammation of the Human Dental Pulp Induces Phosphorylation of eNOS at Thr495 in Blood Vessels.

The activity of endothelial nitric oxide synthase (eNOS) in endothelial cells increased with the phosphorylation of the enzyme at Ser1177 and decreased at Thr495. The regulation of the phosphorylation sites of eNOS at Ser1177 and Thr495 in blood vessels of the healthy and inflamed human dental pulp is unknown. To investigate this, healthy and carious human third molars were immersion-fixed and decalcified. The localization of eNOS, Ser1177, and Thr495 in healthy and inflamed blood vessels was examined in consecutive cryo-sections using quantitative immunohistochemical methods. We found that the staining intensity of Ser1177 in healthy blood vessels decreased in inflamed blood vessels, whereas the weak staining intensity of Thr495 in healthy blood vessels strongly increased in inflamed blood vessels. In blood vessels of the healthy pulp, eNOS is active with phosphorylation of the enzyme at Ser1177. The phosphorylation of eNOS at Thr495 in inflamed blood vessels leads to a decrease in eNOS activity, contributing to eNOS uncoupling and giving evidence for a decrease in NO and an increase in O2- production. Since the formation of the tertiary dentin matrix depends on intact pulp circulation, eNOS uncoupling and phosphorylation of eNOS at Thr495 in the inflamed pulp blood vessels should be considered during caries therapy.

Longitudinal craze line propagation in human root dentin after instrumentation with NiTi rotary files of different instrument tapers after long-term chewing simulation.

OBJECTIVES: The aim of this study was to investigate whether file design and taper significantly influence microcrack initiation during machine preparation. MATERIALS AND METHODS: Sixty extracted teeth with straight single canals were selected. The teeth were randomly assigned to four groups based on their root canal anatomy and the corresponding NiTi rotary file system (I, Mtwo; II, ProTaper Universal; III, F6 SkyTaper; control, no preparation and filling). The root canals of the experimental groups were filled using the single-cone technique. The tested teeth were all subjected to a mechanical chewing simulation with flat lead loading over a period of 3 years (corresponding to 150,000 cycles). The teeth were checked for dentinal defects (accumulative crack growth in length) under the digital microscope (Keyence VHX-5000) at time 0 (baseline prior to chewing simulation) and after 3, 6, 12, 24, and 36 months of loading. The cumulative crack increase was statistically analyzed using the Kruskal-Wallis test, Jonckheere-Terpstra test, and the Wilcoxon rank-sum test. The significance was set at p < 0.05. RESULTS: In contrast to preparation with greater-tapered instruments, ProTaper Universal (group II) and F6 SkyTaper (group III) instrumentation with the smaller tapered Mtwo files (group I) showed less accumulative propagation of craze lines (p < 0.05) at all time points. CONCLUSION: Instruments with greater taper for root canal instrumentation should be used with care to avoid negative long-term effects in the form of propagation of dentinal defects over time. A positive cutting-edge angle and a smaller taper have a positive effect on a lower craze line development. CLINICAL RELEVANCE: Instruments with a positive cutting-edge angle and a smaller taper are beneficial for the long-term preservation of dentinal tooth structure.

Seven-year clinical performance of CEREC-2 all-ceramic CAD/CAM restorations placed within deeply destroyed teeth.

OBJECTIVES: Adhesively luted all-ceramic restorations represent a promising way to preserve and stabilize weakened tooth substance, but little information is published about the clinical performance of extensive all-ceramic restorations. MATERIALS AND METHODS: A total of 78 large CEREC 2™ single-tooth all-ceramic restorations had been placed in 35 patients. After 7 years, 59 teeth in 25 patients were reevaluated according to USPHS or modified USPHS criteria regarding aesthetic properties, e.g., "anatomic form," "color match," and "marginal discoloration"; functional properties, e.g., "marginal integrity," wear expressed by the criteria "proximal contact" and "static/dynamic occlusal relationship"; and biological properties, e.g., "tooth vitality" and "secondary caries". Additionally, the "proportion of margin below/above cemento-enamel junction" was included. RESULTS: Two restorations had failed prior to the 7-year recall, one due to a bulk fracture of the restoration and one due to poor marginal integrity (rated "Charlie") after 4 years. Other six restorations were rated as failure at the 7-year evaluation (three restorations revealed secondary caries, one was bulk fracture of the Cerec 2 restoration, and two failures were related to endodontic problems resulting in extraction or amputation of one root, respectively), resulting in a failure rate of 13.1% after 7 years. A total of 96.4% of the restorations revealed sufficient ratings for esthetic properties "anatomic form," "color match," "marginal discoloration," and "marginal integrity". CONCLUSIONS: The survival rate of 86.9% at the 7-year recall demonstrates that adhesively luted all-ceramic CAD/CAM-generated restorations are suitable for restoration of extended coronal defects. CLINICAL RELEVANCE: CAD/CAM-generated all-ceramic restorations facilitate the reconstruction of deeply destroyed teeth irrespectively of the location of the cavity margins.