ABSTRACT
LF82, an adherent invasive Escherichia coli pathobiont, is associated with ileal Crohn's disease, an inflammatory bowel disease of unknown etiology. Although LF82 contains no virulence genes, it carries several genetic differences, including single nucleotide polymorphisms (SNPs), that distinguish it from nonpathogenic E. coli. We have identified and investigated an extremely rare SNP that is within the highly conserved rpoD gene, encoding σ70, the primary sigma factor for RNA polymerase. We demonstrate that this single residue change (D445V) results in specific transcriptome and phenotypic changes that are consistent with multiple phenotypes observed in LF82, including increased antibiotic resistance and biofilm formation, modulation of motility, and increased capacity for methionine biosynthesis. Our work demonstrates that a single residue change within the bacterial primary sigma factor can lead to multiple alterations in gene expression and phenotypic changes, suggesting an underrecognized mechanism by which pathobionts and other strain variants with new phenotypes can emerge.
ABSTRACT
La presente investigación se centra en el análisis sociedadnaturaleza y los rasgos culturales y tradicionales en el municipio de Cuetzalan, Puebla, particularmente, en el uso de plantas (herbolaria de la región) y prácticas tradicionales con fines curativos, por lo tanto, una investigación de este tipo puede contribuir a conseguir una estrategia de rescate cultural ligado a la conservación de la biodiversidad.
