Selective distribution and quantitation of T-lymphocyte subsets in germinal centers of human tonsils. Definition by use of monoclonal antibodies.

The distribution and quantitation of T-lymphocyte subpopulations of human tonsils were studied in situ by the avidinbiotin-peroxidase technique, using monoclonal antibodies to total, helper, and suppressor T cells. Primary follicles contained few T lymphocytes. The germinal centers of secondary follicles contained numerous T cells: in the periphery, 14.2% +/- 1.7% (mean +/- SD), and in the central area, 2.4% +/- 0.7%, of follicular lymphocytes counted were T lymphocytes. The mantle zone had fewer T lymphocytes (7% +/- 0.9%). Most of the T lymphocytes found in the germinal centers had the helper-cell phenotype. There were fewer than 1% of suppressor T lymphocytes in the germinal centers. An occasional secondary follicle could contain up to 10% of suppressor cells (range, 0% to 9.7%). The interfollicular areas contained preponderantly T lymphocytes, a majority of which reacted with monoclonal antibodies to helper cells. The distribution and enumeration of T lymphocytes in normal reactive lymphoid tissue provide a basis for studies of pathological specimens.

Prosthetic rehabilitation following major nasal resection.

Treatment of extensive carcinoma of the external nose has as an objective the eradication of disease and rehabilitation of the patient. We review our experience with 15 patients treated with total and subtotal rhinectomy. Prosthetic rehabilitation offers prompt recovery of both cosmesis and function without morbidity. We conclude that prosthetic reconstruction of major nasal defects deserves consideration when rehabilitation following rhinectomy is required.