ABSTRACT
Foaming is a key issue should be solved in the process of photo-fermentation biohydrogen production (PFHP), since it has negative influence on the hydrogen yield potential, especially when taken straw as substrate. Appropriate foam control measures must be considered for industrialization. Hence, in this work, foam height and biohydrogen yield were selected as index, the effect of defoamer addition on PFHP was investigated. The defoamer has no negative effect on bacterial growth. In the addition range of 0-1 mL/L, the higher addition amount, indicates better foam control effect. The maximum foam height could be reduced by 55% and the foam existence time by 36 h. The reduction of foam was beneficial to biohydrogen production, and the highest cumulative hydrogen yield was increased 23% at the addition level of 0.125 mL/L.
Subject(s)
Hydrogen , Fermentation , Hydrogen-Ion ConcentrationABSTRACT
Paulownia biomass is rich in carbohydrates, making which a potential feedstock for biohydrogen production. In the study, different parts and varieties of Paulownia were chose as substrates to evaluate hydrogen production potential of paulownia lignocellulose via biohydrogen production by photo fermentation (BHPPF) and energy conversion efficiency (ECE). Results showed the highest cumulative hydrogen yield (CHY) of 67.11 mL/g total solids (TS) and ECE of 4.74% were obtained from leaves of Paulownia, which were 121.06% and 115.45% higher than those of the branches. Moreover, Paulownia jianshiensis leaves were found to be the best variety for BHPPF, with the maximum CHY of 98.83 mL/g TS and ECE of 7.18%. Using Paulownia waste as the substrate to produce hydrogen helps broaden the range of raw materials for BHPPF and improve the economic utilization of forestry waste.
Subject(s)
Hydrogen , Lignin , Biomass , Fermentation , Hydrogen/analysisABSTRACT
In the paper, Use Chlorella as raw material, HAU-M1 Photosynthetic bacteria (PSB) as hydrogen-producing bacteria, the influence of initial pH on bio-hydrogen by photosynthetic organisms from Chlorella vulgaris with diverse enzyme addition was studied. The results showed that when using cellulase as hydrolase, the optimum initial pH was 7.0 and highest bio-hydrogen was 25.99 mL/g dry cell weight. Using neutral protease as hydrolase, the optimum initial pH was 8.0 and highest bio-hydrogen was 16.47 mL/g dry cell weight. Using mixed enzyme of cellulase and protease as hydrolase, the optimal initial pH was 7.0 and highest bio-hydrogen was 27.43 mL/g dry cell weight. The bio-hydrogen from Chlorella after mixed enzymatic hydrolysis is better than that of single enzymatic hydrolysis, we think the mixed enzymatic hydrolysis of cellulase and protease was superior to the single enzymatic hydrolysis of the two enzymes, which provides a scientific reference and low-cost bio-hydrogen technology by microalgae.