ABSTRACT
A novel PCR method was developed to discriminate amongst genotypes A-C of the rotavirus non-structural protein 4 (NSP4). Genotype-specific primers were designed that correctly identified the NSP4 genotype when evaluated as a multiplex PCR with cell culture adapted rotavirus strains. Rotavirus strains B223 SGIG6P6[1], NCDV SGIG6P6[1] and SA11 SGIG3P5B[2] were used as control for NSP4 genotype A; A34 SGIG5P14[23], Gottfried SGIIG4P2B[6] and Wa SGIIG1P1A[8] for NSP4 genotype B; RRV SGIG3P5B[3] for NSP4 genotype C. Subsequently, the same set of specific primers was used to genotype a set of 77 Swedish clinical samples. The results showed that all human clinical samples analyzed belong to the NSP4 genotype B and the VP6 subgroup II.
Subject(s)
Glycoproteins/genetics , Polymerase Chain Reaction/methods , Rotavirus Infections/virology , Rotavirus/classification , Rotavirus/genetics , Toxins, Biological/genetics , Viral Nonstructural Proteins/genetics , Adolescent , Adult , Antigens, Viral/genetics , Base Sequence , Capsid Proteins/genetics , Child , DNA Primers , Genotype , Humans , Molecular Sequence Data , Rotavirus/isolation & purification , Sequence Alignment , SwedenABSTRACT
Rotavirus is a major cause of acute gastroenteritis. By examining 1,517 stool samples collected in 2001 and 2002 from Swedish adults with acute diarrhea, rotavirus was found in 3.2%, with the emerging G9P[8] serotype being the one most commonly identified (42.9%). This is the first documentation of G9 infections in adults in Europe.
